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Correction to: ITR-Seq, a next-generation sequencing assay, identifies genome-wide DNA editing sites in vivo following adeno-associated viral vector-mediated genome editing
BMC Genomics volume 21, Article number: 810 (2020)
Correction to: BMC Genomics 21, 239 (2020)
Following publication of the original article , we noticed that incorrect datasets were used for some analyses. We have corrected Figs. 2, 3, S1, and S2, Table S2, and dataset S1. Access to additional supporting data files via the webpage has also been restored. Importantly, the conclusions of the manuscript are not affected by this error.
The corrected figures, data files and a summary of the corrections (Additional file 5) to the text have been included with this Correction article.
Breton C, Clark PM, Wang L, et al. ITR-Seq, a next-generation sequencing assay, identifies genome-wide DNA editing sites in vivo following adeno-associated viral vector-mediated genome editing. BMC Genomics. 2020;21:239. https://doi.org/10.1186/s12864-020-6655-4.
Frequency of AAV integration in the on- and off-target sites. The number of ITR-Seq reads for the on and off-target sites are shown as a percentage of the total number of ITR-Seq reads before the filtering step (see Methods). Analysis was performed on the ITR-Seq results for liver biopsies at d17/d18 and d128/d129 from non-human primates treated with the indicated nuclease and AAV dose.
Distribution of mismatches between the target sequence and identified off-target sequences. Off-targets sequences were extracted from the ITR-Seq results for AAV-M1PCSK9 (at a dose of 3 × 1013 or 6 × 1012 GC/kg, panels a and b) and AAV-M2PCSK9 (6 × 1012 GC/kg dose, panels c and d) groups at d17/d18. Thirty-one top-ranked (according to the number of ITR-Seq reads) off-target sequences, with a length of 22 bp and with no more than 10 mismatches, were retained for analysis. Location of the off-target sites are shown on the left and mismatches between the off- and on-target sequences are highlighted. The data to generate the WebLogo (43) shown on top were the selected off-target sequences for each group multiplied by the reported number of ITR-Seq reads (Dataset S1).
. ITR-Seq rank of GUIDE-Seq-identified off-target events.
Summary of Corrections.
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Breton, C., Clark, P.M., Wang, L. et al. Correction to: ITR-Seq, a next-generation sequencing assay, identifies genome-wide DNA editing sites in vivo following adeno-associated viral vector-mediated genome editing. BMC Genomics 21, 810 (2020). https://doi.org/10.1186/s12864-020-07039-2