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Table 2 Summary of Illumina and PacBio output data quality and assembled sequences of libraries of neem

From: Multi-tissue transcriptome analysis using hybrid-sequencing reveals potential genes and biological pathways associated with azadirachtin A biosynthesis in neem (azadirachta indica)

Sequencing Platform Sample Reads Number (M) Clean Bases (G) Q20 (%) Q30 (%) Total number of unigene Total length of unigene (bp) Mean length of unigene (bp) N50 GC (%)
Ilumina HiSeq Leaf 41.14 6.17 96.95 92.30 50394 82508501 1380 2201 40.64
Flower 41.35 6.20 97.29 93.06 62426 95564612 1530 2335 40.13
Stem 40.60 6.09 97.29 93.11 65762 98612970 1499 2372 40.39
Fruit 40.59 6.09 97.45 93.39 66668 81799852 1226 2077 41.93
Root 40.64 6.10 97.44 93.43 45459 57083335 1255 2100 41.10
Hiseq summary 113008 175268545 1550 2599 40.99
PacBio SMRT Mixed tissue 6.75 11.28 22884 82035635 3584 5068 43.55
Calibrated PacBio by Illumina   20201 72872459 3607 5076 43.55
  1. Q20 and Q30 on Illumina platform correspond to the predicted base call error rate of 1% and 0.1%, respectively
  2. N50: The minimum contig length needed to cover 50% of the transcriptome