Fig. 5

Spatiotemporal expression of BmSuv4–20 and BmDNMT2. a Relative expression of BmSuv4–20 and BmDNMT2 in the 3rd-d of fifth instar stage of larvae of Bombyx mandaina and B. mori. b, c Temporal expression profiling of these genes during testis (b) and ovary (c) development. BmSuv4–20 and BmDNMT2 are highly expressed during the meiotic division of spermatocytes, from the start of the fifth instar stage of the larvae to the start of wandering (L500–W0) (b) and the meiotic division of oocytes, from the 7th d of pupa to 2 h after laying eggs (P7–E2h) (c). L500–L506: The start to the 6th d of the fifth instar stage of larvae, W0–W2: The start to the 2rd d of the wandering stage of larvae, P1–P9: The 1st d to the 9th d of the pupa, M0: The start of moth, and E10m–E2h: 10 min to 2 h after laying eggs. Sw22934 was used as a reference; three replicates were used per time point