Fig. 2

Cyclic genes in each organ: Circadian genes within each organ were identified by rain algorithm (p-value < 0.01). Each heatmap represents the circadian gene expression patterns of all genes in that organ over a 24-h period. Lists of circadian gene names and expression levels for each time point are provided in Supplemental Table 3). Gene expression read counts were first normalized to library size to generate CPM values. Mean of CPM values between biological replicates were subsequently calculated to represent expression level of a gene in an organ at a particular Zt, followed by scaling of gene expression at each Zt to the mean expression of a gene throughout a day. The scaled expression levels were represented by color (i.e., red: higher expression; green: lower expression). Circadian genes were ordered by peak expression time and length of time for a gene to reach the minimum expression