Fig. 7

Yeast two-hybrid and BiFC assays of interactions between candidate Md14–3-3 proteins and MdTFL1/MdFT. a Yeast two-hybrid assays. MdTFL1–1, MdFT, and Md14–3-3 proteins were fused to the GAL4 DNA-binding domain (BD) or activation domain (AD) to generate the bait constructs or prey constructs. The empty pGADT7 vector was used as control. -LT, yeast medium lacking leucine and tryptophan. -LTAH, yeast medium lacking leucine, tryptophan, adenine and histidine. b, c BiFC assays in the Nicotiana benthamiana leaves. Interactions between Md14–3-3 s and MdTFL1–1 (b), and MdFT (c), respectively. MdTFL1–1 and MdFT coding regions were cloned into pSPYNE respectively, and MdGF14a, MdGF14d, MdGF14i, and MdGF14j coding regions were independently cloned into the pSPYCE vector. The empty pSPYCE vector served as the control. The negative control of empty pSPYNE vector was shown in Additional file 7: Figure S3. Fluorescence was imaged by laser scanning confocal microscopy (LEICA TCS SP8, Germany). The YFP fluorescence, chlorophyll autofluorescence (CHl), and bright-field images were merged. Bar = 25 μm