From methylglyoxal to pyruvate: a genome-wide study for the identification of glyoxalases and D-lactate dehydrogenases in Sorghum bicolor

Table 3 Information on domain organisation of SbGLYI proteins for the prediction of enzymatic activity and metal ion dependency

Protein	Protein domain (PF00903)			Presence of conserved metal binding sites^*				Predicted enzymatic activity	Metal ion dependency Ni²⁺/Zn²⁺
Protein	Start	End	Length	H/Q	E	H/Q	E	Predicted enzymatic activity	Metal ion dependency Ni²⁺/Zn²⁺
SbGLYI-1.1	5	133	128	+	–	+	–	Absent	–
SbGLYI-1.2	7	124	117	+	–	+	–	Absent	–
SbGLYI-2	9	133	124	–	+	–	–	Absent	–
SbGLYI-3	273	394	129	+	–	–	–	Absent	–
SbGLYI-4	52	174	123	+	–	+	+	Absent	–
SbGLYI-5	21	140	120	+	–	+	+	Absent	–
SbGLYI-6	215	371	157	+	–	–	–	Absent	–
SbGLYI-7	85	205	121	+	+	+	+	Present	Ni²⁺
SbGLYI-7	216	335	120	+	+	+	+	Present	Ni²⁺
SbGLYI-8.2	72	214	144	+	+	+	+	Present	Zn²⁺
SbGLYI-8.1	57	201	144	–	+	+	+	Present	Zn²⁺
SbGLYI-9	19	142	123	+	–	+	+	Absent	–
SbGLYI-10	25	144	121	+	+	+	–	Present	Ni²⁺
SbGLYI-10	161	277	124	+	+	+	+	Present	Ni²⁺
SbGLYI-11	29	149	121	+	+	+	+	Present	Ni²⁺
SbGLYI-11	161	281	124	+	+	+	+	Present	Ni²⁺
SbGLYI-12	70	185	116	+	+	+	+	Absent	–
SbGLYI-13	26	144	119	+	–	+	–	Absent	–
SbGLYI-14	92	212	128	+	+	+	+	Present	Ni²⁺
SbGLYI-14	224	339	128	+	+	+	+	Present	Ni²⁺
SbGLYI-15	7	121	114	–	+	–	–	Absent	–

* ‘-’ indicates absence of the corresponding residue and ‘+’ indicates its presence

ISSN: 1471-2164