Fig. 4

Putative cis-elements in RcAP2/ERF promoters. The promoter region of RcAP2/ERF genes shows cis-elements (1500 bp upstream). The assessment was done using PlantCARE, a nucleotide sequence pattern database for plant cis-acting regulatory DNA components. Symbols show binding sites. The left panel showed the different AP2/ERF subgroup members. Heatmap represented different cis-elements of different RcAP2/ERFs promoter regions. MBS I, MYB binding site involved in flavonoid biosynthetic genes regulation; E2F, cis-acting element involved in the cell proliferation, differentiation, and endocycle; MSA-like-element, a cell cycle-related element; P-Box, gibberellin-responsive element; TCA-element, cis-acting element involved in salicylic acid responsiveness; AT-rich element, the essential elements of replication origins of bacterial replicons; ABRE, cis-acting element involved in the abscisic acid responsiveness; CGTCA-motif, cis-acting regulatory element involved in the MeJA-responsiveness; TGACG-motif, cis-acting element involved in the abscisic acid responsiveness; AuxRR-core, cis-acting element involved in the auxin responsiveness; TGA-element, auxin-responsive element; TATC-box, gibberellin-responsive element; MBS, MYB binding site involved in drought-inducibility; TC-rich repeats, cis-acting element involved in defense and stress responsiveness; LTR, cis-acting element involved in low-temperature responsiveness; DRE, cis-acting element involved in dehydration, high salinity, or low temperature; WUN-motif, wound-responsive element; MYC, cis-acting element involved in the dehydration and abscisic acid responsiveness; W box, WRKY binding site; MYB, cis-acting element involved in the dehydration and abscisic acid responsiveness; STRE-element, stress-responsive element; WRE3-element, cis-acting regulatory element involved in high temperature. Three different types of cis-acting elements (plant growth and development, phytohormone responses, and abiotic and biotic stresses) are represented by different colors, as shown on the right