Fig. 8

Expression analysis of osmotic and ABA-related genes in VC and RcDREB2B overexpressed lines. Analysis of the osmotic stress-responsive genes and ABA-related genes in VC and RcDREB2B transgenic lines was performed using RT-qPCR. Total RNA extraction was carried out from 3-week-old seedlings grown under normal growth conditions. Three separate replicates were carried out for RT-qPCR analysis. The relative expression of individual genes of VC was set to 1.0, and AtACTIN2 was used as an internal control gene. An additional file (Additional file 2: Table S2) lists the primers used. Different letters indicate significant differences in three independent experiments at P < 0.05 using one-way ANOVA analysis