Fig. 10From: Transcriptome analysis and molecular mechanism of linseed (Linum usitatissimum L.) drought tolerance under repeated drought using single-molecule long-read sequencingValidation of differentially expressed genes by RT-PCR. a-d Gene expression changes of GAPDH, DNA repair, MAPK signalling pathway, proline biosynthesis and photosynthesis genes under CK, DS, RW and RD in Z141 and NY-17. M indicates the DL2000 DNA marker; NC indicates negative control and CK, DS, RW, and RD indicate control check, drought stress, re-watering and repeat drought, respectively. Note: This image has been optimized by reasonable cropping, the original and full-length gel images please see additional file 24Back to article page