Fig. 7
From: Transcriptome analysis of the transdifferentiation of canine BMSCs into insulin producing cells
a The verification of the 20 key genes by qRT-PCR. b-d Overexpression of VIP, SSTR2 and RPS6KA6 respectively, 48 h after transfection. e-g Knockdown of VIP, SSTR2 and RPS6KA6, the bright fields were merged with fluorescence images. (scale bar = 50 μm) h qRT-PCR of OE-VIP and KD-VIP after induction, “*“means 0.01 < p value < 0.05, “**” 0.001 < means p value < 0.01, “***” means 0.0001 < p value < 0.001, “****” means p value < 0.0001. i qRT-PCR of OE-SSTR2 and KD-SSTR2 after induction. j qRT-PCR of OE-RPS6KA6 and KD-RPS6KA6 after induction. k The GSIS results of overexpression and knockdown of VIP, SSTR2 and RPS6KA6 and normal induction groups are control samples