Fig. 3

A much greater number of protein-coding genes are differentially expressed during infection with the highly virulent Toxoplasma RH strain than with the less virulent PTG strain. Wild type BMDM were infected with either the highly virulent RH strain or the less-virulent PTG strain, and 6 h later RNA was isolated for sequencing. Differentially expressed mouse transcripts were identified based on statistical significance (PPDE greater than 0.95) and a fold change of greater or less than 2. a Classification of differentially expressed mouse transcripts as either protein-coding, non-coding, pseudogene, or TEC (To be Experimentally Confirmed). b Total number of protein-coding transcripts of higher or lower abundance during infection. c Venn diagrams of differentially expressed protein-coding transcripts showing shared and unique expression patterns between infection strains. d Heat maps displaying trends among functionally related genes. Experiments were performed in at least triplicate with BMDM preparations from separate mice