Table 5 Proteins of E. nipponicum participating in the haem synthesis pathway
Enzyme | Transcripts/TPM | Function |
|---|---|---|
5-Aminolevulinic acid synthase (ALAS) | No hit | Formation of 5-aminolevulinic acid (ALA) by condensation of succinyl-CoA and glycine in the mitochondria |
5-Aminolevulinic acid dehydratase (ALAD) | 2/31.61 | Formation of porphobilinogen (PBG) from ALA after exporting to the cytosol |
Porphobilinogen deaminase (PBGD) | 1/8.90 | Condensation of four PBGs to the unstable hydroxymethylbilane (HMB) |
Uroporphyrinogen III synthase (UROS) | 1/13.46 | Cyclisation of HMB to uroporphyrinogen III (URO III) |
Uroporphyrinogen III decarboxylase (UROD) | 3/55.85 | Decarboxylation of four acetic side chains to methyl groups to form coproporphyrinogen III (COPRO III) |
Coproporphyrinogen III oxidase (CPO) | 1/12.32 | Formation of protoporphyrinogen IX (PP’gen IX) back in the mitochondria |
Protoporphyrinogen oxidase (PPO) | 2/14.95 | Oxidation of PP’gen IX yielding protoporphyrin IX (PP IX) |
Ferrochelatase (FC) | 2/5.11 | Catalyse the insertion of a ferrous ion into PP IX, creation of the final product (the haem) |