Fig. 5

Phylogenetic analysis of 4141 single-copy BUSCO orthologs from F. poae isolates investigated in this study including Belgian isolate 2516. F. venenatum assembly GCA_900,007,375.1 was included as an outgroup to determine the true location of root (double slashes indicate truncated branch leading to outgroup). Evolutionary histories were inferred using the Maximum Likelihood (ML) method. Branch lengths are measured in number of substitutions per site, and node values indicate Ultrafast bootstrapping values (n = 1000). Biosynthetic gene annotations: the FpAPS cluster and the disrupted FpNRPS4 (Ψ) synthetase are associated with accessory chromosome sequences in the Fp157 assembly, whereas the disrupted FpPKS2 (Ψ) synthase was assembled to a core chromosome in Fp157. Purple and green pie charts represent size of fragments detected relative to concatenated Fp157 FpNRPS4 (Ψ) or PKS2 (Ψ) sequences. Empty pie charts indicate absence of FpNRPS4 (Ψ) detection