Fig. 5

Heat map of hierarchically clustered floral development unigenes. Absolute values of transcript per million normalized gene expression ranged from a minimum of two-fold to > 8000-fold change in expression relative to controls (FDR p < 0.05). Columns represent log₂-fold expression values of all biological replicates in inflorescences at 120 cm of development (120P), developing boot stage florets (FT), pre-anthesis florets (PAF), stamens (ST), and florets at anthesis (ANT) (Table 5). Clustering utilized transcript per million (TPM) normalized expression values to iteratively calculate pair-wise Manhattan distances between all clusters and joining clusters of proximity. Branch length represents the distance between clusters and reflects the similarity of expression profiles for co-expressed genes within the two clusters