Fig. 7

Heat map of hierarchically clustered seed development unigenes. Absolute values of transcript per million normalized gene expression ranged from a minimum of two-fold to > 8000-fold change in expression relative to controls (FDR p < 0.05). Columns represent log₂-fold expression values of all biological replicates of developing florets at anthesis (ANT), immature seed (IS), and mature seed (MS) (Table 5). Clustering utilized transcript per million (TPM) normalized expression values to iteratively calculate pair-wise manhattan distances between all clusters and joining clusters of proximity. Branch length represents the distance between clusters and reflects the similarity of expression profiles for co-expressed genes within the two clusters