Fig. 8
From: Reproductive developmental transcriptome analysis of Tripidium ravennae (Poaceae)
Validation of RNA-Seq expression levels with qPCR. Black and red lines represent relative gene expression levels observed in the RNA-Seq and qPCR respective datasets. RNA-seq data are transcript per million (TPM) normalized means ± SEM. Relative gene expression of qPCR data utilized using BI1-like protein gene and translationally controlled tumor protein homolog TCTPH as reference genes and calculated with the 2(-ΔΔCt) algorithm. a Trav0002740 (caffeic acid 3-O-methyltransferase). b Trav0032568 (NUCLEAR FUSION DEFECTIVE 4 isoform X2). c Trav0016008 (PARTING DANCERS isoform X1). d Trav0008006 (CHROMOSOME TRANSMISSION FIDELITY 7). e Trav0015779 (RNA-DIRECTED DNA METHYLATION 4). f Trav0014920 (POLLEN DEFECTIVE IN GUIDANCE 1)