Fig. 6

Sodalis transcriptomic profiles within teneral tsetse flies. A Scatterplot with TPM distribution within each tsetse midgut library; x axis is in log₁₀ scale, only genes with TPM > 0 are plotted (left). Dots around the median indicate TPM values of individual genes. Bar graphs partition the percentage of the total number of genes according to their expression levels (low expression < 100 TPM, high expression > 100 TPM or no expression with TPM = 0) with corresponding percentage of genes with > 100 TPM indicated by the call-outs on the bottom of each corresponding bar (right). Across midgut libraries of both tsetse species, the Sodalis isolates exhibit low gene expression. B PCA analysis indicates that 41.1% of the variability across Sodalis expression may be accounted for by tsetse host species. C Output of differential expression analyses to obtain the consensus set of DE genes between tsetse species isolates. The intersect at the center of the Venn diagram contains the genes found to be differentially expressed by the four informatic approaches indicated on each oval (n = 235). D-E Average TPM from three gut RNASeq libraries of selected Sodalis genes involved in the metabolism of chitin; asterisks indicate significant differential expression (*; p < 0.0001)