Fig. 3

Identification of key module based on WGCNA. a Correlation between co-expressed WGCNA module eigengenes and phenotypic traits. Depth of color corresponds to depth of correlation. Positive correlation indicated in red and negative correlation indicated in blue. Significance (P-value) of each module to each external factor presented in parentheses (). b Enrichment map of 438 genes. c Modules found by MCODE in the network related to erythropoiesis. The edge width was proportional to the score of protein-protein interaction based on the STRING database, The color of edge was weight acquired from WGCNA. d The heatmap of erythropoiesis-related gene expression in RNA-seq. e qRT-PCR analysis. The mRNA expression levels of 10 selected genes were normalized with the external control gene (Gapdh) and were calculated with 2^−ΔΔCt