Table 1 Number of reads assigned using BLAST with cockatiel samples and control sample

Total reads

8,842,628

8,160,360

8,503,438

14,041,743

18,929,276

Eukaryota

(% total reads)

8,420,210

(95.2)

8,148,391

(99.9)

8,354,957

(98.3)

13,951,068

(99.4)

18,839,539

(99.5)

Bacteria

(% total reads)

76,253

(0.9)

1,614

(<0.1)

144,494

(1.7)

80,294

(0.9)

57,770

(0.3)

Chlamydia psittaci

(% bacteria)

51,147

(67.1)

109

(6.8)

142,521

(98.6)

-

-

Campylobacter jejuni

(% bacteria)

24,072

(31.6)

1,387

(85.9)

1,158

(0.8)

78,015

(97.2)

-

Enterobacteriaceae

-

32

-

-

2,823

Clostridium colinum

149

-

-

-

-

Salmonella enterica

-

-

-

-

19,573

Raoultella ornithinolytica

-

-

-

-

14,815

Citrobacter sp.

-

-

-

-

10,401

Escherichia coli

-

-

-

-

6,158

Methylophaga sp.

-

-

-

-

1,179

Lysinibacillus sphaericus

-

-

-

-

1,045

etc.

-

-

-

-

1,776

unassigned

885

118

815

2,279

3

Virus

(% total reads)

2,582

(<0.1)

3,530

(<0.1)

809

(<0.1)

-

13,145

(<0.1)

retroviridae

2,514

3,420

752

-

-

hepatitis B virus

4

-

57

-

-

herpesvirus

-

110

-

-

-

Escherichia phage

-

-

-

-

11,275

Enterobacteria phage

-

-

-

-

1,617

Streptococcus phage

-

-

-

-

222

Rous sarcoma virus

-

-

-

-

31

Unidentified

(% total reads)

343,083

(3.9)

6,825

(0.1)

3,178

(<0.1)

10,381

(0.1)

31,967

(0.17)