Table 1 Lambs generated with MSTN knockout after optimization of the CRISPR/Cas9 system in vitro
No. of donor ewes | 33 |
No. of collected embryos | 358 |
Cas9 mRNA:sgRNA | |
 No. of microinjected embryos | 350 |
 No. of transferred embryos | 345 |
 No. of recipient ewes | 58 |
 No. of pregnancies | 14 |
Newborns | 16 |
 No. of homozygous knockouts | 4 |
 No. of non-homozygous knockouts | 4 |
 No. of wild-type lambs | 8 |