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Table 1 Lambs generated with MSTN knockout after optimization of the CRISPR/Cas9 system in vitro

From: Optimized Cas9:sgRNA delivery efficiently generates biallelic MSTN knockout sheep without affecting meat quality

No. of donor ewes 33
No. of collected embryos 358
Cas9 mRNA:sgRNA
 No. of microinjected embryos 350
 No. of transferred embryos 345
 No. of recipient ewes 58
 No. of pregnancies 14
Newborns 16
 No. of homozygous knockouts 4
 No. of non-homozygous knockouts 4
 No. of wild-type lambs 8