Fig. 4

Enrichment analysis using the DAVID bioinformatics tool was focused on genes differentially methylated in sperm from low- versus high-fertility bulls. As a reference, the list of all genes covered by reduced representative bisulphite sequencing (19,962 genes) was used. (A) The first diagram (EASE score 2.07) represents genes clustering across categories related to Pleckstrin homology-like domain and Rho guanine nucleotide exchange factor (B) The second diagram (EASE score = 1.89) represents a cluster of genes in categories related to ATP and nucleotide binding activity. (C) The third diagram (EASE score = 1.85) represents a cluster of genes in categories related to lipid metabolism and degradation (D) The fourth diagram (EASE score = 1.78) represents a cluster of genes in categories polymorphisms and splicing. Default settings of the DAVID bioinformatics tool were applied and clusters of genes with EASE enrichment score > 1.3 were considered as significant. The blue colour illustrates that the listed gene occurred within each category