Fig. 8

Flowering time phenotypes of the VrCCA1L26 transgenic Arabidopsis plants. a Flowering time of VrCCA1L26 transgenic and wild-type Arabidopsis plants. Arabidopsis plants were grown in the growth chamber under 16 h 23 °C light/8 h 21 °C dark cycle conditions. Bars = 4 cm. b The number of rosette leaves in VrCCA1L26 transgenic and wild-type plants. The rosette leaf numbers of VrCCA1L26 transgenic and wild-type plants were recorded after the bolting of Arabidopsis plants. c The expression levels of VrCCA1L26 in VrCCA1L26 transgenic and wild-type plants. The expression level of VrCCA1L26 in transgenic line 1 was set to 1, and the values for other transgenic and wild-type plants were adjusted accordingly. Significant differences relative to the corresponding wild-type plants are indicated with asterisks (**P < 0.01). d-f The expression levels of CO (d), FT (e), and SOC1 (f) in VrCCA1L26 transgenic and wild-type plants. The x-axis indicates the light and dark cycle throughout the day. The expression levels of CO, FT, and SOC1 were normalized to AtActin. Open boxes indicate the time of lights-on, and black boxes indicate the time of darkness. ZT, Zeitgeber Time (ZT)