Table 3 RT-qPCR primers of miRNA for cumulus cells and Granulosa cells
Types | Transcript ID | Primer sequences (5′-3′) |
|---|---|---|
| Â | miR-2431-y miR-7977-X | F: CACCCCCACTTGCATGACCCTGA F: TTCCCGGCCAACGCACCA |
miRNA | miR-2284-Z miR-365-y miR-7862-y miR-342-y miR-195-X miR-574-X U6(XM_021614042.1) | F: AAAAAGTTCGTTTGGGTTTTCT F: TAATGCCCCTAAAAATCCTTAT F: TGGTGCTCCCTGGAGCTGAGC F:TCTCACACAGAAATCGCACCCATC F: TAGCAGCACAGAAATATTGGCA F: TGAGTGTGTGTGTGTGAGTGTGT F: GGAACGATACAGAGAAGATTAGC R: TGGAACGCTTCACGAATTTGCG |