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Fig. 3 | BMC Genomics

Fig. 3

From: Profiling the transcriptomic signatures and identifying the patterns of zygotic genome activation – a comparative analysis between early porcine embryos and their counterparts in other three mammalian species

Fig. 3

Comparative analysis of transcriptome activity between porcine SCNT and IVV embryos. A Schematic showing the preparation of porcine SCNT embryos for RNA-seq. For 2-cell, 4-cell and 8-cell embryos were collected by SCNT. RNA sequencing was performed using pools of 10 oocytes/embryos (3 replicates per group). B Principal component analysis was performed for all developmental stages with their replicates. Each point represents a replicate. C, D Heatmaps and cluster analysis showing the global gene expression levels between SCNT and IVV embryos at the 2-cell, 4-cell and 8-cell stages. E Volcano plot of differentially expressed genes (DEGs) between porcine IVV 2-cell and MII oocytes stage, 4-cell and 2-cell stage embryos, 8-cell and 4-cell stage embryos, and blastocyst and 8-cell stage embryos (FC > 2, FDR < 0.05)

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BMC Genomics

ISSN: 1471-2164

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