Gene expressions between obligate bamboo-eating pandas and non-herbivorous mammals reveal converged specialized bamboo diet adaptation

Table 3 Primers for the PCR amplifications of NR3C1 protein coding region

Primer	Primer sequence	Length	Anneal temperature	Extension time
GR-head-F	5’CTGATGATAATCTCTGATGGACTC3’	197 bp	55℃	12 s
GR-head-R	5’TTTGCTGCTTGGAATCTGACTG3’	197 bp	55℃	12 s
GR-F	5’ATGGACTCCAAGGAATCACTAAG3’	1048 bp	54℃	1 min
GR-1R	5’GTTGTTGAGAAAGGGATGCTGTATT3’	1048 bp	54℃	1 min
GR-3F	5’CAGAGAAAGAGGCGAGTGAGAGTC3’	705 bp	58℃	42 s
GR-3R	5’TCCCACAAGTCAAGACCCCGTAAT3’	705 bp	58℃	42 s
GR-6F	5’CTCAGGGTGTCATTACGGGGTCT3’	963 bp	55℃	1 min
GR-8R	5’TCAATACTCATGGTCTTATCCAA3’	963 bp	55℃	1 min
GR-9F	5’TCTGTTCATGGTGTGAGTACCTC3’	690 bp	57℃	42 s
GR-9R	5’GTCATACCCTGCGTATAACACC3’	690 bp	57℃	42 s
GR-11F	5’AAAACCTTACTGCTTCTCTCTTC3’	578 bp	56℃	36 s
GR-11R	5’TATCACTCTCTACTCTTCCCACC3’	578 bp	56℃	36 s

ISSN: 1471-2164