Fig. 1
Validation of putative genes predicted to be directly regulated by GlnR. A The relative binding levels of GlnR on the upstream regions of putative genes determined by ChIP-qPCR. The binding levels in ΔglnR were arbitrarily set to 1.0. N+ and N- indicate nitrogen excess and limited condition, respectively. Error bars indicate SD from three independent experiments. **p < 0.01; * < 0.05. B EMSA of upstream regions of selected genes. Each lane contained 0.3 nM labeled probe. Lanes 2 to 5 contained 0.5, 1, 1, and 1 μM His6-GlnR respectively. Lanes -: EMSAs without His6-GlnR. A 200-fold excess of unlabelled specific probe (lanes S) or nonspecific competitor DNA (Probe 1) (lanes N) was used in competition assays. The original blots are shown in Fig. S1