Fig. 4

Different nitrogen-metabolism-involved gene expression and nitrogen source utilization of WT and ΔglnR. A Differential expression of genes involved in nitrogen metabolism determined by KEGG enrichment. B Growth curve of WT and ΔglnR. Solid line represents the growth under nitrogen-excess condition; dash line represents the growth under nitrogen-limited condition. Error bars indicate SD from three independent experiments. C Growth status of WT and ΔglnR in minimal medium with 30 mM NH₄Cl, glutamate, glutamine, urea, NaNO₂, or NaNO₃ as sole nitrogen source. Error bars indicate SD from three independent experiments. **p < 0.01; *p < 0.05