Fig. 3

Phylogenetic tree (a), conserved domain (b), conserved motifs (c), and exon/intron organization (d) of BBX gene family from S. spontaneum. Protein sequences were aligned by ClustalW and the tree was constructed by MEGA X software using the neighbor-joining method, with 1000 bootstrap replicates. Bootstrap values below 70% are not shown. Black solid triangles are the new BBXs found in Saccharum. The B-box domains and CCT domain are highlighted by green and yellow boxes, respectively. Motifs of each of the SsBBXs, and 10 different motifs, are each denoted by different colored boxes. Exons and introns are represented by yellow boxes and black lines, and untranslated (UTR) 5′ - and 3′ -regions are indicated by green boxes, respectively