Table 1 List of primers for quantifying microbial genes from endometrial fluid samples
Primer Set | Primers | Sequence | Application |
|---|---|---|---|
16 S rRNA | Forward | 5′-GTGCCAGCMGCNGCGG-3′ | qRT- PCR |
Reverse | 5′-GGGTTNCGNTCGTTG-3′ | ||
Enterococcus | Forward | 5′-GGATTGCCTCAAGAAGCGACTG-3′ | qRT- PCR |
Reverse | 5′-AAGCGATCACTGCGTTCATAGAC-3′ | ||
Salmonella enterica | Forward | 5′-CCTGAATCACTTACCCGTCAAACTG-3′ | qRT- PCR |
Reverse | 5′-ACGCCATTCGCCATAATCTCAAC-3′ | ||
Rickettsiales | Forward | 5′-GTTGGTTGTACTGCTGGTCCTAAG-3′ | qRT- PCR |
Reverse | 5′-ACTCTTCATTACCTTGTAGCCATCC-3′ |