Fig. 6

Expression analysis of GhUSPs in leaves under salt treatment via RT–qPCR. The samples were collected at 0 h, 1 h, 3 h, 6 h, 9 h, 12 and 24 h after salt treatment on TM-1 cotton seedlings. GhActin7 was used as an internal reference. Error bars represent the standard deviation of three independent biological replicates. Statistically significant difference was evaluated by one-way ANOVA analysis (*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001). The primers used are listed in Table S3