- Research article
- Open Access
Conserved loci of leaf and stem rust fungi of wheat share synteny interrupted by lineage-specific influx of repeat elements
BMC Genomicsvolume 14, Article number: 60 (2013)
Wheat leaf rust (Puccinia triticina Eriks; Pt) and stem rust fungi (P. graminis f.sp. tritici; Pgt) are significant economic pathogens having similar host ranges and life cycles, but different alternate hosts. The Pt genome, currently estimated at 135 Mb, is significantly larger than Pgt, at 88 Mb, but the reason for the expansion is unknown. Three genomic loci of Pt conserved proteins were characterized to gain insight into gene content, genome complexity and expansion.
A bacterial artificial chromosome (BAC) library was made from P. triticina race 1, BBBD and probed with Pt homologs of genes encoding two predicted Pgt secreted effectors and a DNA marker mapping to a region of avirulence. Three BACs, 103 Kb, 112 Kb, and 166 Kb, were sequenced, assembled, and open reading frames were identified. Orthologous genes were identified in Pgt and local conservation of gene order (microsynteny) was observed. Pairwise protein identities ranged from 26 to 99%. One Pt BAC, containing a RAD18 ortholog, shares syntenic regions with two Pgt scaffolds, which could represent both haplotypes of Pgt. Gene sequence is diverged between the species as well as within the two haplotypes. In all three BAC clones, gene order is locally conserved, however, gene shuffling has occurred relative to Pgt. These regions are further diverged by differing insertion loci of LTR-retrotransposon, Gypsy, Copia, Mutator, and Harbinger mobile elements. Uncharacterized Pt open reading frames were also found; these proteins are high in lysine and similar to multiple proteins in Pgt.
The three Pt loci are conserved in gene order, with a range of gene sequence divergence. Conservation of predicted haustoria expressed secreted protein genes between Pt and Pgt is extended to the more distant poplar rust, Melampsora larici-populina. The loci also reveal that genome expansion in Pt is in part due to higher occurrence of repeat-elements in this species.
Plants and pathogens are in a constant struggle as each co-evolves to adapt to genomic changes. Plant genomes are adapting to different modes of infection by pathogens while pathogens are evolving different avenues to circumvent defense systems of their respective hosts. Rust fungi are among the most economically important pathogens, yet are part of elusive host-pathogen systems. The order Pucciniales (formerly Uredinales or Urediniomycetes) contains over 7,000 different species from 100 genera . Adding to the complexity, individual cereal crops can be infected by several rust fungi adapted to the specific crop.
Cereal rust fungi are obligate biotrophs and have alternate hosts where sexual recombination takes place, allowing for diversification of the population . The life cycle of cereal rust fungi begins with a urediniospore landing on a leaf surface and germinating in the presence of adequate humidity. A germtube emerges and moves towards a stomate via a thigmotrophic response and probable chemical clues  where an appressorium will form. A hypha grows inside the substomatal space until a mesophyll cell is encountered. The fungus will penetrate the cell wall and produce a haustorium by invagination of the plasma membrane [4, 5] At each stage of infection, the fungus is postulated to secrete effectors to inhibit cell defenses and reprogram cells to redirect nutrients. Though some candidate effectors are shared among the rust fungi, most are specific to their host and include transcription factors, zinc finger proteins, small secreted proteins and cysteine-rich proteins . Certain classes of effectors, such as ones modulating host immunity, are believed to rapidly change to overcome resistance, however, the mechanisms generating this variation are not known. In several studied pathogens, certain classes of predicted effectors are found in variable and highly mutagable regions of the genome. Mobile elements induced mutations in effectors in Phytophthora, Magnaporthe, and Leptosphaeria while Fusarium oxysporum has a specialized chromosome with effectors [10, 11]. Effectors can be clustered in the genome (Ustilago;) including at telomeres (Fusarium,; Magnaporthe;). Avirulence genes from the flax rust fungus, Melampsora lini are all small secreted proteins [15, 16]. Currently, two effectors have been identified in urediniospores of Puccinia graminis f.sp. tritici (Pgt) that induce the in vivo phosphorylation and degradation of the barley resistance protein, RPG1 .
Sequencing technology has made significant advancements in recent years. Complete genomes of more species, including fungi, are being sequenced. Comprehensive catalogs of genes can be generated, annotated, and comparisons made to other genomes. Core sets of genes needed for function, adaptations for life cycle, and host specificity can now be found. Comparisons of several obligate fungal plant parasites have identified common losses of genes involved in nitrate and sulfur metabolism [6, 18]. Melampsora larici-populina (Mlp) and Pgt have approximately 8,000 orthologous genes which could be suggested as a core set needed for biotrophism. However, 74% and 84% of the secreted proteins, respectively, are lineage specific  suggesting proteins that are needed for the individual life cycle. Corn pathogens, U. maydis and S. reilianum are also closely related and share 71% of effector genes in so-called divergence clusters. However, 10% are U. maydis specific while 19% are specific to S. reilianum.
Puccinia triticina (Pt) is the causal agent of wheat leaf rust and new races emerge each year aided by a crop monoculture placing a strong selection pressure on the pathogen. Genetic variation is generally believed to increase through sexual recombination to generate new allele combinations. Two related wheat rust fungi, Pgt and P. striiformis f.sp. tritici (Pst), causing stripe rust, have a sexual cycle on North American Berberis spp. and have a greater race variability where the alternate host is present . The Pt aeciospore stage is on Thalictrum spp. and Isopyrum, found mainly in the Mediterranean region, however, other Thalictrum species present in North America can support a reduced level of infection  but are generally resistant to Pt[22, 23]. Populations are essentially asexual; supported by the lack of recombination found in numerous North American races [24–28]. A parasexual cycle may exist allowing recombination since germtube fusion, nuclear migration, and bridging structures between nuclei have been observed in Pt.
The obligate biotrophic nature of cereal rusts makes experimental manipulation difficult, however, genomics provides a means of studying evolution and gene function. We set out to understand the genome variation of two rust fungi at three regions. A Pt bacterial artificial chromosome (BAC) library was made and clones were identified using three probes that would isolate regions of predicted secreted proteins and avirulence. Sequenced DNA regions of Pt were compared to syntenic regions in two rust species with complete genome sequences, Pgt, and Mlp, and evaluated for genomic conservation, expansion and mutations.
BAC library construction
Urediniospores harbor two haploid nuclei with an estimated total genome complexity for Pt of approximately 135 Mb, based on comparative DNA fluorescence (L. Szabo, unpublished) and the current total size of the genome assembly (http://www.broadinstitute.org/annotation/genome/puccinia_group/GenomeStats.html). The generated P. triticina BAC library contained 15,360 clones arrayed in 384 well plates with an average insert size of 105 kb representing an estimated 10 to 12 genome equivalents. A single-copy probe identified nine positive clones on high density filters, and assuming fragments were randomly cloned during library construction, this is in agreement with the estimated genome coverage.
BAC clone selection, sequencing and characterization
Three genomic regions were targeted for comparison. Previous work had mapped a Pgt RAD18 homolog in a genomic region harboring an avirulence gene . Using PgtRAD18 as a reference, PT0313.J16.C21 (GenBank accession number GR497566) was identified from a Pt EST database using TBLASTN (E value = e-107; [31, 32]) and used as a probe. Nine positive BAC clones were found and clone 1F16 (Pt1F16) was selected for sequencing because of its longer length and the centralized location of PtRAD18 within the BAC clone. Sequences from Pt1F16 were assembled into two contiguous sequences of 39,219 and 63,874 bp, totaling 103,093 bp (GenBank JX489506). The GC content of these sequences was 47%. Subclones were generated spanning the gap for orientating and ordering of the two contigs. However, due to a region of 60 near-perfect 46 bp repeats of ACCAGCCCGCCGAGAGGAAGCCCTCTCGGCGAGCTGGTGTGTGTAT, the gap could not be closed. FGENESH, with gene models from the Puccinia group genome project (http://www.broadinstitute.org/annotation/genome/puccinia_group/), predicted 30 open reading frames (ORFs) ranging from 210 to 4,077 bp in length.
In a functional screen of Uromyces viciae-fabae, secreted peptide effector protein UF5 was related to the flax rust Melampsora lini haustorial-expressed secreted protein HESP-379 . A Pgt genome search revealed several predicted secreted protein homologs in close proximity, suggesting the presence of small clusters of predicted secreted proteins. UF5 (Genbank ES608162) aligned with two predicted secreted proteins, PGTG_03708 (E score 5 e-33) and PGTG_03709 (E score 0), both transcribed and located 513 bp apart on the Pgt contig. Using these Pgt sequences, PtContig18 (Genbank accession HP451841) and PtContig7347 (Genbank accession HP458556) were identified by a BLASTN Pt EST database search. A PCR product from the cDNA clone, Pt EST PT0061b.D10.TB that aligned to Contig18 (GenBank accession EC400508), was used as a probe to identify Pt BAC PtHSP02. Sequencing of this BAC resulted in four assembled contigs. Gaps could be spanned and thus the contigs could be ordered and oriented. Sizes of the contigs in bp were 16,991, 30,055, 5,014, and 60,277 for a total of 112,337 bp (GenBank JX489507). Gaps were present in regions of repeated DNA and could not be assembled. GC content was 46.3% and FGENESH predicted 31 ORFs in the contig ranging from 174 bp to 7,167 bp in length. The smaller ORFs were generally within repeated elements.
The bean rust effector UfHSP42c Uf011 (GenBank ES608167; ) matched three predicted protein sequences in Pgt, PGTG_17547 (E Score 0), PGTG_17548 (E Score 1 e-21) and PGTG_17549 (E Score 1 e-4). UfHSP42c matched five Pt ESTs, including clone PT0131d.B10.BR (GenBank accession EC414978) from which probes were derived to identify Pt BAC clone HSP04. Sequencing of HSP04 produced two contiguous sequences of 9,276 bp and 157,027 bp for a total of 166,303 bp (GenBank JX489508). GC content was 46.3% and 61 ORFs were predicted ranging from 120 bp to 5,214 bp in length.
The predicted ORFs from each BAC clone were aligned using BLASTN to the Pgt genome, Pgt predicted transcripts and Pt ESTs, and using BLASTX, to the Pgt, Mlp, and U. maydis (Um) predicted proteomes (Table 1). Pt1F16 had nine ORFs with synteny in Pgt. Identity across the protein sequences ranged from 37-87% in these alignments and putative annotations could be assigned to five of the proteins. Pt1F16-4 contained many gaps when compared to PGTG_13013. Proteins Pt1F16-5, 6, 7, 8 and 9 aligned with two proteins each from Pgt. Pt1F16-7 aligned with PgtRAD18, which has one copy in each of the Pgt haplotype genomes. All but one homolog could also be found in Mlp and four were represented in Um (Table 1).
Nine predicted proteins in PtHSP02 were confirmed through EST sequence alignment  and a putative function could be assigned to eight of them. Alignment identity ranged from 30-100% in PtHSP02. Eight homologs could be found in both Mlp and Um in PtHSP02. The most highly conserved protein is PtHSP02-6, a G-protein ß-subunit containing a conserved WD-40 repeat motif. The first 343 amino acids were 100% identical to PGTG_03727 and 99% to Mlp accession GL883091 (Table 1). Conversely, PtHSP02-3 was only 30% identical to PGTG_3706 and had no homologs in the other two fungi. PtHSP02-4 and PtHSP02-5 aligned with Mlp HESP-379, the haustorial expressed predicted secreted protein homolog from M. lini, and a homolog was found for each in Pgt (Table 1, Figure 1). Two insertions/deletions were found in PtHSP02-4 and PGTG_3708 (Figure 1A). PtHSP02-5 and PGTG_3709 aligned to homologs from M. lini, Mlp, M. medusae deltoidis, and U. maydis. The N-terminal half of the protein was conserved between Puccinia and Melampsora (Figure 1B). There appeared to be 48 genus-specific amino acid changes across the protein. Um was the most diverged with only a few conserved motifs.
Fourteen predicted proteins were identified in PtHSP04 and could be supported through EST sequence alignment. Every protein had a homolog in Pgt with protein identities ranging from 26-95% (Table 1); nine could be assigned a putative function. Eight PtHSP04 proteins had homologs in Mlp and five in Um. PtHSP04-1, 5, and 14 appeared to be unique to Pt with little homology to Pgt. The predicted transcripts of PtHSP04-6, 7, 8 and 9 aligned to a single EST of P. striiformis predicted to encode a secreted protein (ADA54575; ) at scores of 4 e-5, 2 e-8, 6 e-48, and 3 e-9, respectively. PtHSP04-6 and 7 aligned both to PGTG_17549, though revealing 26 and 60% identity, respectively. The predicted HSP04-7 ORF is 1,095 bp in length and contains a 3’ in-frame repeat of nine nucleotides, GG(C/T) AC(T/A) AC(T/A), translating to 30, three amino acid repeats of Gly-Thr-Thr. Without the repeat, PtHSP04-7 is a homolog to PGTG_17549, while PtHSP04-6 is unique to Pt. PtHSP04-8 and 9 are responsible for the homology to Uf-HSP42c and isolation of the BAC clone (Figure 2, Table 1). They are very highly identical except for the C-terminal 18 amino acids, where PtHSP04-9 has a five amino acid deletion and only four identities (Figure 2). Each aligned to PGTG_17547 and PGTG_17548, adjacent proteins which themselves are 100% identical. PtHSP04-8 and 9 are 76% and 71% identical to PGTG_17547, respectively (Figure 2, Table 1).
Repetitive elements and repeated sequences
Each BAC was evaluated for repeat elements by using REPBASE against Pgt, Pt and Pst genomes. Complete and incomplete terminal inverted repeats (TIR), LTRs, Copia, Gypsy, Mariner, Mutator, Harbinger, Helitron, hAT, and DNA transposons were found. (Additional file 1: Tables S1 and S2). Major insertions are represented in Figure 1. Copia elements were found inserted within Gypsy elements in Pt1F16 and PtHSP02. PtHSP02 and PtHSP04 also had localization of LTRs.
To investigate whether the high number of candidate orthologs with Pgt maintained the same gene order, the Pt BAC sequences were aligned to the available Pgt contig sequences. Figure 3 graphically represents the location along each BAC clone of Pt ORFs with EST sequence or protein homology support. The majority of Pt1F16 aligned to the 325,000 bp to 415,000 bp region of Pgt scaffold (SC) 40 but also to the 5,000 to 65,000 bp region of PgtSC110. PgtSC40 and PgtSC110 could either represent the two Pgt haplotypes or a duplication of this region in the genome. Overall, gene order was maintained in both scaffolds. As previously noted, eight of the Pt1F16 ORFs aligned to homologs in Pgt but Pt1F16-1 to 3 were found only on PgtSC40 (Table 1, Figure 3A). Pt1F16-1 aligned to PGTG_12990 85 kb upstream in SC40 of PGTG_13012 whereas Pt1F16-2 and 3 were similarly spaced as their counterparts on this Pgt SC. Between Pt1F16-4 and 5, four retrotransposons were found, of which one was similar to a retroelement in PgtSC110. No mobile elements were found in this region on PgtSC40. PtRAD18 (Pt1F16-7) is a single ORF while Pt1F16-8 aligned to an ORF corresponding a cysteine rich SCP family protein in both SCs of Pgt.
PtHSP02 aligned to a single scaffold, PgtSC7 (Figure 3B). A second haplotype was not detected as the Pgt assembly represents most loci with a single sequence . Nine Pt ORFs could be aligned to homologs on PgtSC7 (position 1,135,000 to 1,280,000). As with the other BAC clones, gene order was generally maintained. However, PtHSP02-1 and PtHSP02-2 were found embedded between retroelements and LTRs. While PtHSP02-1 aligned to two fragments on PgtSC7, PtHSP02-2 was 48% homologous to a gene on PgtSC15 elsewhere in the genome. The remaining genes in PtHSP02 were in the same order as on PgtSC7, except a large insertion of approximately 70 kB of DNA, including sequence similar to mobile elements, was found between PGTG_03709 and PGTG_03708 on PgtSC7. Additional PgtSC7 DNA insertions were evident within this gene cluster whereas the Pt homologs were packed in a tighter arrangement. Across this region, a higher number of retrotransposon elements were found on PtHSP02 (Additional file 1: Table S1).
PtHSP04 aligned to at least six regions within the Pgt genome and represents the least syntenic sequence amongst the three BAC clones (Figure 3C). PtHSP04-1 and 2 were found on PgtSC84, however, there were several repeat elements within both the Pt and Pgt regions. PtHSP04-3 appeared to be a fragmented ORF because a single homologous ORF was found on PgtSC35. PtHSP04-4 and 5 were found on two separate scaffolds, PgtSC4 and PgtSC48, respectively. PtHSP-6, 7, 8, and 9 have homologs on Pgt SC89 in the same order and similar gene distance (conserved micro-synteny). PtHSP04-10, flanked by an LTR and Harbinger element, does not have a homolog on PgtSC89, but on PgtSC13. Microsynteny of PtHSP04-11, 12, and 13 to Pgt is maintained. PtHSP04-14 is a single copy gene in Pt but is repeated in Pgt. between BAC positions 60,000 and 125,000 there are a high number repeat elements.
One of the most interesting sets of sequences were Pt ORFs for which numerous homologous copies were found in the Pgt genome but were not classified as typical mobile elements (identified by small letters in Figure 3; Table 2). Twenty of these ORFs had repeats in the Pgt genome numbering from 19 to 474. Table 2 lists the conserved amino acid domains, if present, in each of the ORFs and the percent identity, which. ranged from 34-74%. Each ORF was compared to an RNAseq cDNA library of Pt-infected leaf tissue (Fellers and Bruce, unpublished) and nine aligned to the experimental cDNA sequences. The predicted proteins were analyzed for peptide content and most had an abundance of Lys, which is suggestive of helical structures. Each of the proteins was also compared to the PHYRE 2.0 structural data base  resulting in seven that revealed regions that aligned, with confidence, to known structures. The first 191 peptides of PtHSP04-j had a structure similar to RAD54, with 99.7% confidence. Of note, PtHSP04-e was expressed and was 51% identical to a protein in Mlp.
This study was performed to look at three regions of the Pt genome that were hypothesized to be under selection pressure because of the presence of putative secreted proteins or loci associated with avirulence. To begin with, gene order is conserved between the Pt BACs and Pgt. However, there is a wide range of protein conservation. A previous comparison of ESTs of Pt and Pgt found a similar level of variation in sequence, but only 40% of the Pt EST unigenes had orthologs in Pgt. Many genes were likely missing in the unigene set because of the difficulty of sampling other Pt life stages to sufficient depth, affecting the percentage. Nevertheless, within the BAC clones, many protein identities were supported by ESTs and similar sequence variation was present . Some proteins were highly conserved between the two wheat rust fungi and had homologs in Mlp and Um.
The three genes used for identifying the BACs were of most interest, in particular, the amount of variation within the sequence. PgtRAD18 had been associated with an avirulence locus in Pgt. PtRAD18 protein length is relatively similar but the sequence has diverged from the PgtRAD18 with only 56% identity. Structurally, PtRAD18 is still closely associated with a predicted secreted protein. Pt has two genes similar to HESP-379 from M. lini. Two indels in PtHSP02-4 suggest a recombination event or splicing difference evolved since the two species diverged, while the sequence differences in the C-terminus of PtHSP02-5 suggest that this region could be very variable. PtHSP04 contained a four-gene locus predicted to code for secreted proteins. Two of them are unique while two are recently duplicated paralogs. Secreted proteins are believed to be most variable amongst fungal proteins because they are under the highest selection pressure to avoid recognition by the host [16, 19, 37]. At least with these examples, It can be said that sequence variation, recombination, and duplication are driving the changes in these proteins.
Numerous fungal genomes have recently been generated, analyzed, and published. Now comparisons can be made to find core gene families associated with specific life styles and cycles. In an extensive comparison, Duplessis et al.  identified core conserved genes needed for biotrophic life in both rust species. It appears that PtHSP02-6 may be one of those genes. PtHSP02-6 aligns with a G-protein beta subunit (GPBS) and no peptide differences were found between Pt and Pgt. Furthermore, there is little difference between Pt and Mlp suggesting that this protein is under strong purifying selection in rusts. Yet, the genes flanking PtHSP02-6 are relatively conserved indicating strong selection and the importance of this gene. In Verticillium dahliae, mutations in GPBS had reduced virulence, increased microsclerotia and conidiation and decreased ethylene production . GPBS is also involved in similar functions in F. oxysporum. In M. grisea, GPBS mutants could not form appresorium, and hyphae could not penetrate and grow in rice leaves . The authors also showed that by over expressing GPBS in the fungus, appressorium could form on a hydrophillic surfaces suggesting that GPBS is necessary for control of surface recognition, growth and appressorium formation . Surface recognition and appressorium formation are the key to rust fungal establishment. This suggests that PtHSP02-6 is indispensable for the biotrophic lifecycle and could be a regulating link in pathogenicity.
A strong correlation between genome size and repetitive element content has been found for many fungal genomes. Genome expansion is significant between Pt and Pgt, even though they are both closely related and are both dikaryotic. The assembled genome for Pgt is 89 Mb  while Pt is currently estimated to be 135 Mb (Broad Institute). The sequence analysis of the three BAC clones gives some indication on why the Pt genome may be larger than the Pgt genome. Pt1F16 had the least mobile element complexity, but had Gypsy elements within Copia elements, as did PtHSP02. PtHSP02 also harbored numerous TEs and LTRs in the region between PtHSP02-1 and 3. Meanwhile, PtHSP04 contains more non-TE repeat ORFs, its homologous genes are scattered across Pgt scaffolds, and its sequence reveals recombination and/or transposition events disrupting syntenic genes. There is also evidence of gene movement by active elements. PtHSP02-2 was directly flanked by LTRs and was not found in PgtSC7, PtHSP04-5 was also flanked by LTRs and could be found in PgtSC48, and PtHSP04-10 only had a single LTR flanking it, but was flanked on the opposite side by a partial Harbinger element. It is possible that since these regions are in repetitive sequence there are assembly errors in Pgt, however, each Pgt homolog are in high confidence scaffolds.
Most surprising are the non-transposable element, repeated sequences found in the Pt BACs (Table 2). Each had homologs throughout the Pgt genome. Most had conserved domains that were maintained, while flanking sequences were greatly diverged. Many were high in Lys suggesting a helix protein structure. Some are expressed, based on the presence of an aligning EST, and have homologs in Mlp, suggesting an importance. The helical nature of these proteins would suggest their involvement as nucleotide binding elements. Pt has five different spore types in its lifecycle involving two different hosts requiring a significant level of cell modifications and cell types. Sequences like these have not been described before and could represent undiscovered elements in the disease cycle.
This work has shown significant genome synteny between two closely related wheat rust fungi. Gene sequences confirmed previous findings of the existence of EST sequence variation between Pt and Pgt. Various levels of homologies are present, but many of the genes are diverging in a manner that is species specific . Both genomes have a significant amount of mobile elements. Some TE copies are conserved between the two species suggesting ancestral insertion. The insertion of TE sequences helps explain genome expansion, and their insertion near secreted protein genes may alter their regulation or cause their duplication and spread or deletion. Most surprising was the presence of small predicted non-TE genes with numerous homologs in Pgt. As many of the small repeated sequences are highly helical in predicted structure, one could suggest they are involved in DNA binding and regulation. Further work is needed to determine when they are expressed and at what stage of the life cycle. When analysis of the Pt and Pst genomes has been concluded, it can be determined if the repeated nature of these predicted genes is maintained within the wheat rust fungi.
Pt BAC library
Total genomic DNA for the BAC library construction was isolated from P. triticina (Pt) Race1, BBBD  urediniospores collected from susceptible wheat (Triticum aestivum L.) cultivar Thatcher. Spores were increased on plants spray-inoculated with a urediniospore suspension in light mineral oil (Soltrol 170 isoparaffin, Conoco-Phillips Chemical Co, Borger TX). The oil was allowed to evaporate for 30 min, then plants were moved to a dark dew chamber at 20°C and 100% relative humidity for 24 hrs for urediniospore germination and appressorium formation. Plants were grown in a growth chamber under 16-hour day at 20°C. After 10 days, urediniospores were collected and germinated by densely dusting them over sterile water in dishes for 8 hrs using a volatile nonanol solution (1.56 μl nonanol (Sigma-Aldrich, St. Louis MO), 1 ml acetone, 19 ml of ddH2O) spotted on filter paper which was suspended in the lids to stimulate urediniospore germination under crowded conditions. The BAC library was constructed by BioS&T (Montreal, Quebec, Canada; http://www.biost.com). In brief, nuclei were isolated from collected germinated urediniospores and embedded in 1% low melting point agarose plugs. Total genomic DNA embedded in the plugs was partially digested with Hind III, separated by electrophoresis by pulse field gel electrophoresis, and the 100–200 kb region was isolated. After electro-elution and dialysis, the DNA fragments were cloned into the Hind III site of BAC vector pIndogoBAC5 (Epicenter Technologies, Madison, WI) and propagated in E. coli DH10B (Life Technologies, Grand Island, NY).
BAC clone selection and sequencing
The resulting BAC library of 15,360 individual clones was arrayed on nylon membranes. After colony lysis, DNA was bound to the membranes using standard procedures . BAC filters were probed to identify clones for sequencing. Several candidate fragments were selected as probes. The Sfi 1 insert from a Pt cDNA clone, PT0313.J16.C21 (GenBank accession GR497566; ) was labeled with αP32-dCTP using a random primer labeling kit (GE Heathcare, Pittsburg, PA). Positive BAC clones were verified by PCR using primers Forward 5’-AGCTCTTCACACGATTCC and Reverse 5’-ATCTTGGCATTGAGCATC. The second probe, SP02, was amplified from Pt cDNA clone PT0061b.D10.TB (GenBank accession EC400508) by PCR using primers Forward 5’- CTTTCTAGACCTAGGCAACTTAACAC and Reverse 5’- GCGCCATGGACTAGTTGAAGAGGGA. The third probe, SP04 was amplified from cDNA clone, PT0131d.B10.BR (GenBank accession EC414978) using PCR primers Forward 5’-CACGAGGGGAACCGATGGGGGT and Reverse 5’-TGGGTTGGTAAACTATTAATGTGCAC. Southern hybridizations were as described 
Selected BAC clones were sent as a stab culture to the Genome Center at Washington University, St. Louis, MO. BAC clones were cultured, subcloned, shot gun sequenced, and assembled (Washington University Genome Center, St. Louis, MO). Gene calls were made using FGENESH with gene models specific to Puccinia (http://linux1.softberry.com/berry.phtml;). BAC clone gene predictions were compared to Pgt, Mlp and Um genomic resources (http://www.broadinstitute.org/annotation/genome/puccinia_group/Blast.html; http://genomeportal.jgi-psf.org/Mellp1/Mellp1.home.html verified May 7, 2012 and http://www.broadinstitute.org/annotation/genome/ustilago_maydis) using the BLASTN and BLASTX algorithms with settings of E value = 1e-3, Matrix = BLOSUM62, and gapped alignment. Repeats were identified using fungaldb of RepBase 17.04 , containing the repeats of Pt, Pgt and Pst. Long terminal repeats (LTR) were determined by LTR_Finder  (e.g., red arrows in Figure 3).
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Bacterial artificial chromosome
- Pt :
- Pgt :
P. graminis tritici
- Pst :
P. stiiformis f.sp tritici
- Mlp :
Haustoria expressed secreted protein.
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The authors would like to thank Drs. Robert Bowden and Randy Dinkens for their comments in the preparation of the manuscript. JPF is supported by USDA-ARS CRIS 5430-21000-005-00.
The authors declare that they have no competing interests.
GB and JPF conceived the study. GB provided the DNA and constructed the BAC library. BMS and RL designed the primers and isolated the BACs. JPF sequenced the BACs, analyzed the sequence and made gene calls along with MB. CAC analyzed the sequence for repetitive sequence. LS provided the probes for RAD18. JPF, GB, and CAC wrote the manuscript. All authors read and approved the final manuscript.