The diversity of small non-coding RNAs in the diatom Phaeodactylum tricornutum
BMC Genomics volume 15, Article number: 698 (2014)
Marine diatoms constitute a major component of eukaryotic phytoplankton and stand at the crossroads of several evolutionary lineages. These microalgae possess peculiar genomic features and novel combinations of genes acquired from bacterial, animal and plant ancestors. Furthermore, they display both DNA methylation and gene silencing activities. Yet, the biogenesis and regulatory function of small RNAs (sRNAs) remain ill defined in diatoms.
Here we report the first comprehensive characterization of the sRNA landscape and its correlation with genomic and epigenomic information in Phaeodactylum tricornutum. The majority of sRNAs is 25 to 30 nt-long and maps to repetitive and silenced Transposable Elements marked by DNA methylation. A subset of this population also targets DNA methylated protein-coding genes, suggesting that gene body methylation might be sRNA-driven in diatoms. Remarkably, 25-30 nt sRNAs display a well-defined and unprecedented 180 nt-long periodic distribution at several highly methylated regions that awaits characterization. While canonical miRNAs are not detectable, other 21-25 nt sRNAs of unknown origin are highly expressed. Besides, non-coding RNAs with well-described function, namely tRNAs and U2 snRNA, constitute a major source of 21-25 nt sRNAs and likely play important roles under stressful environmental conditions.
P. tricornutum has evolved diversified sRNA pathways, likely implicated in the regulation of largely still uncharacterized genetic and epigenetic processes. These results uncover an unexpected complexity of diatom sRNA population and previously unappreciated features, providing new insights into the diversification of sRNA-based processes in eukaryotes.
Non-protein coding RNAs (ncRNAs) play a critical role in maintaining cellular activity and are engaged in a wide variety of molecular tasks and functions in all studied eukaryotic organisms. For instance, transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs) are essential actors of protein translation, small nuclear RNAs (snRNAs) are involved in the pre-mRNA splicing, nucleolar RNAs (snoRNAs) direct the processing and modification of ribosomal RNAs. In addition, a plethora of other types of ncRNAs of variable size (small RNAs of 20–32 nucleotides up to heterogeneous long ncRNAs of several kilobases) has been reported in different kingdoms of life [1–7]. Although some of these ncRNA species show overlapping features in plants and animals, the information concerning their evolution and functionality is still limited . Until now, the most extensively characterized endogenous ncRNAs are short interfering RNAs (siRNAs), also referred to as endo-siRNAs, and microRNAs (miRNAs), which are generated by the processing of double stranded RNAs (dsRNAs) by a Dicer-class RNase III enzyme [1, 9, 10]. These small RNAs are loaded into RNA-induced silencing complex (RISC) containing Argonaute (AGO) proteins, and guide these protein complexes onto sequence complementary mRNA targets to direct their silencing at the post-transcriptional level through mRNA degradation and/or translation inhibition. Small RNAs, and particularly siRNAs, can also guide AGO-RISC onto sequence complementary DNA targets to direct cytosine DNA methylation and/or chromatin modifications resulting in their transcriptional gene silencing . Besides these small RNA species, a variety of new small RNA classes, involved in novel biological functions such as DNA elimination and DNA repair [12–15], continues to be discovered in different organisms. A class of animal small RNA species that has been recently extensively characterized are PIWI-interacting small RNAs (piRNAs) . These DICER-independent small RNA species are 24-32 nt in length and are derived from long precursor transcripts that originate from distinct genomic regions referred to as piRNA clusters. Mature piRNAs bind specifically to the PIWI subfamily of AGO proteins to promote silencing of transposable elements (TEs) -but also of some protein-coding genes- at the transcriptional and post-transcriptional levels . In mice, this pathway has been mostly characterized in the male germ line, where piRNAs presumably direct transcriptional silencing of TEs through DNA methylation [17, 18], a mechanism that is thus analogous to RNA-directed DNA methylation (RdDM) initially described in plants .
The existence of a sophisticated level of regulation of gene expression involving interactions and cross-talk between different small RNAs, generated by different RNA silencing pathways, becomes more and more apparent . Furthermore, several recent discoveries (e.g., the identification of snoRNAs with microRNA-like function , the finding of small RNAs derived from tRNAs (tRFs) , the co-regulation of miRNA biogenesis and splicing from the same host mRNA ) shed light on even more complex and unanticipated RNA regulatory networks involving various ncRNA populations with different origins and functions.
Marine diatoms represent a valuable biological system to address questions about the evolution and diversification of gene regulatory mechanisms in eukaryotes. This hugely diverse phytoplanktonic group of the Stramenopiles derives from a secondary endosymbiosis event and shows a peculiar genetic makeup [22, 23]. Most significantly, almost half of the diatom proteins have similar alignment scores to their closest homologs in plants and animals and at least 5% of their gene repertoire has been recruited by horizontal gene transfer from bacteria [23, 24]. As a consequence, diatoms possess a unique mix of bacterial, animal and plant metabolic features. About half of the diatom genes encodes for proteins of unknown function, suggesting that many regulators of central processes in diatom biology remain to be identified. Nevertheless, the ecological success of diatoms in the contemporary oceans and their capability to grow in very different environments imply that these organisms evolved sophisticated mechanisms to efficiently regulate genome structure and expression, mechanisms that still await characterization. Comparative analyses of the diatom genomes have indicated transposable elements as key contributing factors to genome diversification [23, 25]. Moreover, the first genome-wide DNA methylation map in the model diatom Phaeodactylum tricornutum has revealed the potential impact of DNA cytosine methylation for the control of transcriptional or post-transcriptional silencing and of differential gene expression under specific growth conditions. The data indicated that around 6% of the genome displays detectable DNA methylation, with extensive methylation being localized over transposable elements . A few studies have also provided an initial characterization of small RNAs in the marine diatoms Thalassiosira pseudonana and P. tricornutum[27–29]. Putative miRNAs have been predicted in these two species, but none of them has so far been experimentally validated. Broadly speaking, the potential regulatory role of small RNAs in diatoms still awaits to be demonstrated. Interestingly enough, we have shown that the expression of anti-sense or inverted repeat sequences of selected target genes can trigger efficient gene silencing in P. tricornutum, a clear indication for the existence of RNA interference-like processes in diatoms. Moreover, we have also described the presence of genes encoding a predicted Dicer-like protein, an Argonaute-like protein and a potential RNA-dependent RNA polymerase (RdRP) in the P. tricornutum genome . RNAi-related proteins have been identified in T. pseudonana as well . Although distantly related to plant or animal RNAi machinery components, these diatom proteins possess conserved structural and functional domains including key amino acid residues that support their potential role in the small RNA pathways described above.
To gain insight into the complexity of small ncRNA biogenesis and function in diatoms, we combined computational and experimental analyses to generate a comprehensive characterization of P. tricornutum small RNA populations. In particular, we carried out a high-throughput sequencing of short RNA fragments extracted from cells grown under different conditions of light and iron, two abiotic factors highly relevant for diatom growth in the oceans [31–33]. The characterization of the sRNA transcriptomes revealed a small ncRNA landscape in diatoms that is much more complex than anticipated. We identified and characterized different functional categories of small RNAs with different sizes, suggesting the presence of distinct biosynthetic pathways. Our study also questioned the occurrence of miRNAs, which were not detected. Instead, it identified highly expressed 21-25 nt small RNAs derived from longer non-coding RNAs such as tRNAs and U2 snRNA, or of unknown origin. The majority of small RNAs correspond to TEs, in particular to the Copia-like diatom-specific retrotransposon family [23, 25]. Finally, a clear correlation between the presence of small RNA/DNA methylation and the silencing of TEs and coding genes was established, suggesting that a small RNA-dependent DNA methylation pathway is operational in diatoms as in other plant and animal organisms. An unprecedented periodic pattern of small RNAs on TEs and coding genes, related to the methylation, is also unveiled by our genome-wide analysis, opening a completely novel line of investigation on the molecular determinants establishing sRNA distribution.
Genome-wide distribution and length distribution of small RNA populations
To characterize the population of P. tricornutum small RNAs (sRNAs), we generated five small RNA libraries within the 18 to 36 nt RNA fraction extracted from cells grown under different conditions: Normal Light (NL), High Light (HL), Low Light (LL), Dark (D), and Iron starvation (−Fe). Their sequencing yielded a total of 8,103,030 reads, which were aligned to the P. tricornutum genome  and quality filtered, resulting in 7,235,310 reads (89%) aligned to at least one genomic location (Table 1). While the conditions we selected were chosen to represent environmental conditions relevant for photosynthetic growth, the agreement of the sequence pool between any two conditions is high (average Pearson correlation of 0.86 on counts per aligned position). We thus decided to focus on sRNAs that were recovered across multiple conditions.
Almost half of the aligned reads (49%) overlaps with repeat regions, followed by a significant fraction located on non-repeat intergenic regions (17%) and coding regions (13%). Notably, 8% of the reads overlap with annotated ncRNAs (Table 1 and Figure 1).
The size distribution of the aligned reads is enriched in three specific lengths, suggesting that distinct sRNA biosynthetic pathways generate the P. tricornutum sRNA populations. The three characteristic lengths mainly correspond to tRNA genes, intergenic regions and repeat elements (Figure 1, top). By assessing the distribution on these 3 types of annotations, we observed: 1. a very abundant RNA population of 24-25 nt in length originating from intergenic regions and, in particular, from a unique 80 bp region on chromosome 2 (see below); 2. tRNA fragments mainly enriched in the 19-20 nt fraction and, to a minor extent, in the 30-33 nt fraction; 3. repeat and coding regions enriched in 25-30 nt fragments (see below TE and methylation section).
An unbiased search for sRNAs reveals diverse classes of novel sRNAs in P. tricornutum
Guided by the three populations that have been highlighted by the analysis of read sizes, we explored the landscape of sRNAs with respect to two kinds of sRNA distribution (see workflow description in Figure 1 and Additional file 1: Table S1). Namely, we aligned reads on the P. tricornutum genome and observed two behaviors. On the one hand, some reads match very specific genome locations and form, after alignment, characteristic piles of thousands of copies of the same sequence, accumulated on a single strand. On the other hand, there are regions, of a few thousand bases in length, that are covered by overlapping reads, accumulated on both strands and, at times, forming several piles distributed with a periodic pattern (see section on transposons).
For the first distribution type (Figure 1A), after a filtering step eliminating low complexity sequences, strand-specific sequences, potential degradation products and other sequences possibly leading to erroneous sRNA prediction (see Methods) [34, 35], we characterized 50 candidate regions that appeared to be specific to sRNAs lying in tRNAs and in intergenic regions. These are organized in three groups. Thirty regions correspond to already annotated non-coding RNA structures, including an highly abundant candidate (representing 0.4% of all aligned reads) that overlaps the U2 snRNA gene located on chromosome 5, and the 5S ribosomal RNA on chromosome 3, as previously noted for human . The majority of the candidate regions overlap with 28 different tRNA sequences (corresponding to 22 different codons), suggesting that they may represent tRNA-related small RNAs (tRFs), similarly to observations made in a few other organisms [37, 38]. The remaining 20 candidates (Additional file 2: Table S2) do not overlap with regions related to non coding RNAs. Two of them resemble miRNA-like molecules, supported by a stable precursor structure, and 18 of them have no particular associated structure. These predictions were well supported across different libraries (28 regions detected in at least two libraries) and on an independent sequencing experiment published by Huang and coworkers  that we integrated in our pipeline (24 regions are also detected).The second kind of sRNAs distribution appeared to be specific to transposable elements and to coding genes (Figure 1B). Three major observations were made: 1. reads overlap over relatively long regions that are typically methylated, 2. the sRNAs accumulation correlates with repression of transcription, and 3. the sRNA profile displays periodic patterns at a distance varying within the 180-200 nt interval.
This analysis highlights that 93.5% of sequence specific reads can be explained by their accumulation in well classified loci covering 8% of the whole genome (Additional file 1: Table S1 reports precise values found in normal light, but the same holds for different environmental conditions). Several of the sRNAs found in our analysis have been selected for experimental validation (Figure 1 and below).
The annotation of processed reads yields two miRNA-like sequences
By combining sequencing data and precursor structure prediction, we predicted only two putative miRNA sequences (see Additional file 3: Figure S1 and Additional file 4: Figure S2 for the predicted structures). To do so, we first assessed the existence of a significantly stable precursor secondary structure (see Methods) around an abundant fragment covered by reads, and then verified that this putative miRNA locus was indeed located over a stem together with a proper star sequence, also represented by read accumulation (Methods and Additional file 3: Figure S1 and Additional file 4: Figure S2).
The most represented putative miRNA locus on chomosome 10 (chr10: 42.6924-42.7420) shows a read profile strikingly consistent with the expectations. Precisely, the precursor sequence is predicted to fold in 3 stems (Additional file 3: Figure S1), suggesting the presence of a polycistronic miRNA cluster as previously reported in other organisms . The first stem contains the main miRNA locus, a sequence that is expressed in all five experimental conditions, and the second stem shows another putative miRNA locus, only expressed under iron starvation. It is noteworthy that both of these putative miRNAs occur together with a star sequence. However, they show 2 nucleotide 3′ overhangs only in one side, which is not compatible with a Dicer processing that normally result in RNA duplexes with 2 nucleotides 3′ overhangs at both 3′ ends , and therefore we named them miRNA-like. A second miRNA-like locus on chromosome 1 (chr1: 2.453.906-2.454.049) is highly overrepresented in all experimental conditions and is supported by a hairpin shaped precursor structure (Additional file 4: Figure S2). However, the read accumulation did not allow the identification of a precise star sequence position. We carried out Northern blot analysis of the identified miRNA-like sequences, but we were unable to detect their expression (data not shown).
In conclusion, although we have predicted two over-represented sequences that may correspond to putative miRNA genes, the general evidence is relatively weak for this class of non-coding RNA, being outnumbered by tRFs (see below) or having only a mild support from RNA structure predictions. Our other attempts to identify bona fide miRNAs from genome-wide analyses were unsuccessful: would it be predictions from sequencing data (MIReNA was used in sequencing data mode ), or sequences alignment to miRBase v.17 . In the latter case, only 0.02% of the sequences could be aligned and they were distributed across a highly diverse set of mature sequences (data not shown). Moreover, none of the miRNAs previously identified in P. tricornotum could be confirmed by our predictions (Additional file 5: Supplementary data). Altogether these results suggest that there might be only very few miRNAs in P. tricornutum, and none of them possess known homologues in other species.
Small RNAs localized in intergenic regions
Among the P. tricornutum sRNAs localized in intergenic regions, the most abundant population of reads (>100,000 reads) is located on chromosome 2 (chr2: 29.039-29.123) (Additional file 2: Table S2). These sRNAs align to a 85 nt-long region that displays a very clear distribution of two overlapping 24 and 25 nt-long fragments, with a plateau at the center of the region (Figure 2A). This specific region concentrates around 8% of all the reads and 55% of the sRNA population matching intergenic regions. These abundant sRNAs have been detected in our five experimental libraries and in the independent dataset by Huang . We can therefore exclude artifactual biases generated during library preparations or sequencing. Furthermore, their expression in vivo was validated by low molecular weight Northern-blot analysis using a 24 nt-long oligonucleotide complementary probe. In agreement with the sequencing data, the analysis detected 22-25 nt-long sRNAs from cells grown under different light conditions and iron starvation that appear as a smear in our analysis. A higher molecular weight band was also detected (Figure 2B) and presumably corresponds to the sRNA precursor. Therefore, like for the miRNAs, we made the hypothesis that these sRNAs could be the result of transcription and precise processing of a longer precursor. However, we failed to amplify this long transcript and to validate its sequence by 5′ and 3′-RACE PCR. Moreover, we did not succeed to predict any stable RNA secondary structure associated to a putative precursor sequence that might be processed to generate the smaller product. Therefore, the biogenesis of the most abundant P. tricornutum sRNAs remains to be determined. Interestingly, a conserved 25 nt-long fragment could also be recovered when analyzing the transcriptome of T. pseudonana (Additional file 6: Figure S3A). As for P. tricornutum, this fragment aligns to an intergenic region of T. pseudonana genome on chromosome 7, suggesting the conservation of this novel small RNA population in diatoms.
Novel populations of U2 snRNA-derived sRNAs
Another sRNA population abundantly represented in our five libraries displayed a very clear distribution over a single 226 nt-long region (chr5: 696.774–696.999) that corresponds to the U2 snRNA gene (Figure 3). U2s are core components of the spliceosomes and are required for accurate pre-mRNA splicing in eukaryotes . We found sRNAs along all the sequence, with two peaks corresponding to two different sRNA populations at the 3′ and 5′ ends of the region: one with a coverage of more than 26,000 reads, localized in the 3′ region of the U2 snRNA (named U2-3′), and another, with a much lower coverage of about 1,200 reads, at the 5′ end (named U2-5′) (Figure 3A). In order to validate their expression, we performed Northern blot analyses. An oligonucleotide recognizing the U2-3′ allowed confirming the existence of two sRNA populations of different sizes (23 and 25 nt), which were clearly detectable in cells grown in High Light (HL) and Low Light (LL). In the other tested conditions, Normal Light (NL) and Dark (D), only the upper 25 nt band was observed. Interestingly, in the iron starvation sample (−Fe) a clear reduction of both bands was observed compared to the other tested conditions (Figure 3B). Finally, the differential expression of the sRNAs matching the 3′ region of the U2 snRNA was further confirmed by stem-loop qPCR  (Figure 3C).
The U2-5′ sRNAs were not detected by Northern blot analysis, probably because of their low abundance compared to U2-3′ (Figure 3A) but their existence and their expression status were confirmed by stem-loop qPCR. The analysis revealed a reduced content of the U2-5′ expression under iron starvation, similar to what was observed for U2-3′ (Figure 3C). Interestingly, qRT-PCR and Northern blot analyses showed a reciprocal upregulation of the U2 snRNA in cells grown under iron starvation condition (Figure 3D). Taken together, these results strongly suggest that the U2-5′ and U2-3′ have a common biogenesis and their content is strongly reduced under iron condition, potentially through the inhibition of the processing of a U2 snRNA common precursor. Neither differences in the U6 snRNA expression nor generation of smaller RNAs associated to this other spliceosome core component were detected, strongly supporting the specificity of the processing generating U2-associated sRNAs. We then mapped the U2-5′ and U2-3′ sRNAs on the U2 snRNA secondary structure (Figure 3E). The U2-5′ is predicted to be part of stem-bulge structure, whereas the U2-3′ matches a hairpin-shaped stem loop-like secondary structure. This latter type of structure might be more efficiently processed to generate the highly expressed U2-3′ products. In order to test this hypothesis, we generated P. tricornutum transgenic lines over-expressing the U2 snRNA (see Methods). An increase in the U2-3′ steady-state level was observed in independent transgenic lines grown under normal light condition, providing a direct link between U2 gene expression and U2-3′ accumulation (Figure 3F). Remarkably, highly expressed small RNAs are also associated to the 3′ end of the U2 snRNA in T. pseudonana (Additional file 6: Figure S3B). This further supports the possibility that U2-3′ sRNAs are generated through the processing of the U2 snRNA precursor transcript through an unknown mechanism possibly conserved in different diatom species.
Small RNAs associated to mature tRNAs
A novel and abundant class of regulatory sRNAs derived from the precise processing of mature tRNAs has been described in organisms as different as human [12, 38], mouse , protozoa [37, 45] and plant . The sequencing of the P. tricornutum small RNA libraries performed in this study revealed a variety of sRNAs related to tRNAs in this microalga as well (Figures 1 and 4; Additional file 7: Table S3). In particular, different sRNA classes of different sizes were detected, likely resulting from different tRNA processing mechanisms: 1. Short fragments of around 19 nt, representing the majority of the P. tricornutum tRFs and found in all the libraries associated to the tRNA 3′ ends (3′-tRFs, 24 cases); 2. short tRFs of around 19 nt matching the tRNA D loop and the anticodon sequences, that do not fit in the general classification scheme of small RNA tRFs  (D loop/anticodon, 9 cases); 3. short tRFs associated to both regions, tRNA 3′ end and D loop/anticodon; 4. longer tRFs (30–35 nt), that were mostly detected under iron starvation (3′-tRFs: 12 cases, to 5′-tRFs: 6 cases) (Additional file 7: Table S3). A fragment matching the full length GluGAG tRNA from the 5′ end to the anticodon loop was also observed, and likely represents a putative half tRNA . Interestingly, the 19 nt-long 3′-tRFs possess the CCA sequence that is added to the 3′ end of the trimmed tRNA intermediates in most eukaryotic organisms . On the contrary, the longer 3′-tRFs do not present the CCA sequence, hinting at the fact that the two 3′ tRFs may originate from different RNA processing mechanisms. tRNA-associated small RNAs have also been detected with a similar proportion (2-4%) in the T. pseudonana transcriptome and with a particular enrichment in 19 and 25 nt-long fragments (Additional file 6: Figure S3C).
We experimentally validated the expression of a total of four P. tricornutum 3′- and 5′-tRFs that showed the highest coverage (Figure 4 and Additional file 7: Table S3) using probes that cover both the short and the long fragments. For all the tested tRNA fragments, we detected by Northern blot analysis the tRFs of 30–35 nt and longer 80–100 nt fragments that may likely represent the mature tRNAs. For the tRF-AspCAG and tRF-ProCCT, a slightly increased expression in cells grown under iron starvation was also observed (Figure 4), which is consistent with the transcriptome analyses. Contrariwise, we had difficulties to detect the shorter tRFs for the tested tRNAs, that become visible only after long exposure of the membranes.
Small RNA-directed control of transposable elements expression
The majority of small RNA fragments correspond to repetitive elements (Table 1) and 98% of the reads that align to repeat regions cover diatom LTR retrotransposons (LTR-RTs) , the most abundant TE family in diatom genomes. These fragments tend to have a length between 25 and 30 nt, cover the entire TE sequence, and are equally distributed along both strands. We observed a consistent coverage of the TE regions across all five libraries (Spearman correlation > 0.89 between any 2 libraries). The sRNA coverage was thus summarized on a region using RPKM , a metric normalization for region lengths and sequencing depth. Most of the regions covered above background level were Copia-like or PiggyBac like TEs (Figure 5A). Nucleotide composition of the reads appears biased at their 5′end, with U being the first nucleotide on almost 60% of the reads (Figure 5B).
Various classes of siRNAs and piRNAs have been reported to regulate the expression of TEs in eukaryotes, either post-transcriptionally or through cytosine DNA methylation and/or other chromatin modifications . To assess if the population of P. tricornutum sRNAs covering the TEs may also have a regulatory role, sRNA coverage was compared to the expression level of the autonomous TEs . Information regarding TE expression was obtained from two independent sources, a set of EST libraries  and a recently published RNA-Seq dataset  both obtained from cells grown under normal light conditions. Therefore, to facilitate further comparisons, we only considered in the following analyses our sRNAs dataset obtained from cells grown under Normal Light condition. For both RNA-seq and EST datasets, the small RNA coverage is significantly higher on TEs with undetectable or low expression (Mann-Withney one sided test, RNA-seq: pvalue < 4e-5, EST: pvalue < 5e-3) (Figure 5C). This suggests that sRNA abundance correlates with TEs characterized by low or no expression, possibly triggered by efficient repression or silencing. This hypothesis was tested by RT-qPCR using primers specific to 7 selected transposons with high (3), medium (1), or low (3) sRNA coverage, respectively. This revealed that autonomous TEs with medium or high sRNA coverage have an expression level at least 1,000 folds lower than the ones with low or no coverage (Figure 5D). This inverse correlation further supports the hypothesis that sRNAs may contribute to the silencing of these TEs. Furthermore, two out of the three TE transcripts with high sRNA coverage could not be amplified, and may therefore be fully silenced.
The distribution of sRNAs associates with DNA methylation in P. tricornutum
Recently, a reference methylome has been established in P. tricornutum by genome-wide profiling of 5-methyl-cytosine . The analysis revealed extensive DNA methylation over transposable elements, which correlated with low expression levels . We made use of this resource to untangle a possible link between the presence of sRNAs and DNA methylation over transposable elements (TEs), as previously described in other organisms . A total of 359 sRNA producing regions overlap methylated TEs, altogether corresponding to 6% genome coverage. These regions are on average longer than 4,000 bp and are covered by 56% of the uniquely mapping reads (Additional file 1 Table S1). Correlation analyses further show that high DNA methylation levels associate with elevated sRNA coverage on autonomous TEs (Mann-Withney one sided test, pvalue < 8e-3 in Figure 5C; Spearman rank correlation = 0.41 in Figure 5E), suggesting that sRNAs could direct DNA methylation at TEs as previously reported in other organisms  (Figure 5E). To further test this hypothesis at the genome-wide level, we further searched for a possible link between TE-associated sRNAs and DNA methylation of all annotated Copia-type transposons on assembled chromosomes (1544 TEs of length > 50). This revealed that the large majority (74%) of the methylated transposons has an RPKM coverage of sRNAs higher than 10 (Figure 5E). The effect is significantly more pronounced on long TEs, with 95% of methylated TEs longer than 1,000 nt (338 TEs) having a RPKM higher than 10. Taken together, these data are fully supportive of a model in which sRNAs direct DNA methylation at many TE loci in P. tricornutum.
Methylated DNA is mainly found on transposable elements in the P. tricornutum genome . However, high DNA methylation levels could also be observed on some coding genes, possibly associated with their transcriptional repression . By comparing the methylation signals encompassing genes with the sRNAs coverage, we also observed a significant positive correlation between methylation levels and sRNAs abundance (Pearson correlation = 0.24) (Additional file 8: Figure S4 and Table 1). This observation further supports a possible role of sRNAs in determining the methylation state of a DNA region on both TE and gene loci. Two main points can be remarked: 1. about 83% of the 3380 Highly Methylated Regions (HMR) overlap with a sRNA-producing locus (76% overlap on more than 75% of their length) and, 2. there is a striking periodic pattern of read placement on the HMRs (Figure 1, right bottom). Fast Fourier Transform analysis was realized on HMRs and a significant period of 180 nt (at times of 90 nt) was revealed (Additional file 9: Figure S5 and Additional file 1: Table S1). Conversely, we did an unconstrained search for regions where the read coverage would have at least three significant periods within a 5000 bp window. We detected 270 regions, which are mostly overlapping HMR (86% or 233 regions) and with an average periodicity between 175 and 225 nt (72% or 196 regions). In the analysis performed, this periodic signal was significantly more pronounced on the HMRs associated with coding genes than on TE regions (Additional file 10: Figure S6A). This first observation rules out the possibility that we observed an artifact generated by repeated regions. As a matter of fact, TE repeats are located in different genomic positions. There, ambiguous mapping of sRNA reads, not necessarily lying on phase, would blur the periodic patterns in coverage signal (see for example Additional file 10: Figure S6B). In conclusion, these analyses permitted to uncover an unprecedented periodic organization of sRNA distribution over multiple genes and TEs heavily targeted for DNA methylation.
Although the annotation of sRNAs in almost all studied organisms is becoming more and more comprehensive, these regulatory elements have only recently been identified in unicellular and multicellular algae belonging to different lineages [53–58]. In this work, we have significantly extended the characterization of the sRNAs from a model Stramenopile species, the marine diatom P. tricornutum, providing new information regarding diversification and function of these elements in eukaryotes. The genome-wide analysis of sRNAs, obtained from cells grown under different light and nutrient stress conditions, revealed the existence of a variety of sRNA populations with characteristic lengths and specific distribution patterns on diverse genomic locations (Figure 1). The functional and in silico characterization of these elements allowed us to reach several novel conclusions.
We questioned the presence of canonical miRNAs in diatoms, previously reported in P. tricornutum. We used a computational approach to look for putative miRNAs in our small RNA dataset, but none of our attempts revealed canonical pre-miRNA-like hairpins that could be substrates for a Dicer-like enzyme. Moreover, none of the sRNAs we identified share significant sequence similarity when compared with microRNA sequences from miRBAse  and miRNAs from Ectocarpus siliculosus, a Stramenopile multicellular brown alga that represents the only example reported for this lineage in the literature with experimentally validated microRNAs [55, 56]. These results strongly indicate that canonical miRNAs, such as those characterized in different eukaryotic organisms so far, are not major regulators of gene expression in P. tricornutum. However, at this stage, we do not rule out the possibility that canonical miRNAs could be identified in response to other environmental cues and/or through a more important small RNA sequencing coverage than the one used in the present study. Nevertheless, we were able to identify some sRNAs located on stable precursor secondary structures, which we designated as miRNA-like because they possess only a part of canonical miRNA features. In particular, an interesting polycistronic miRNA-like cluster has been identified on chromosome 10, with a RNA stem containing a predicted miRNA locus, consistently detected in all our libraries, and another miRNA-like, on another RNA stem, that is observed only in cells under iron starvation conditions. The data suggests that under different environmental conditions, primary transcripts might be differentially processed to generate different miRNA-like elements with different function. Similar examples have been already reported in human . Interestingly, even if these miRNA-like are well represented and predicted to originate from stable structures, we identified 2 nucleotide 3′ overhangs at only one strand of the putative dsRNAs. This observation suggests that this type of small RNAs might be generated via a Dicer-independent mechanism [59, 60]. Alternatively, the P. tricornutum Dicer-like enzyme, which shows a partial sequence conservation with plant and animal Dicer homologues and possesses only a dsRBD domain followed by 2 RNAs III domains  might bind and cleave the opposite strands of a dsRNA in an atypical way to generate the miRNA-like molecules identified in this study. Therefore, further characterization of these miRNA-like elements will likely help identifying novel sRNA biogenesis pathways in diatoms.
Our dataset allowed us to identify the most abundant population of sRNAs in P. tricornutum, showing a clear distribution peak of 24-25 nt in length, localized on an intergenic region of chromosome 2 (chr2: 29.039-29.123). Northern blot analyses experimentally demonstrated the existence of sRNAs in P. tricornutum for the first time (Figure 2B). Their detection in cells grown under different conditions excludes a non-specific RNA degradation origin. Similarly to the miRNAs, these sRNAs may result from the processing of longer RNA precursors, which could successfully be detected. However, despite numerous attempts, we did not succeed to amplify and validate the primary transcript and to identify the dsRNA structure that may generate these products. We can thus hypothesize that, differently from the miRNAs, the precursor is not transcribed by the RNA polymerase II  or is subjected to other 5′ or 3′ end modifications (e.g., decapping and subsequent 5′-3′ degradation), that preclude their amplification using standard RACE. Interestingly, these sRNAs share sequence similarities with sRNAs matching an intergenic region of the T. pseudonana genome. This suggests that novel sRNA populations of unknown origin are conserved between different diatom species and likely evolved to play similar regulatory functions in these microalgae.
Novel results derived by next-generation sequencing experiments have provided some indications about the existence of sRNAs associated to spliceosomal snRNAs  and in mRNA length regulation (telescripting) . The results presented in this study unveiled a previously unknown regulatory network involving U2 snRNA-derived sRNAs. Two different sRNA populations at the 5′ and 3′ ends of the U2 snRNA (the U2-5′ and U2-3′ sRNAs) were identified. We validated the expression of both U2-5′ and U2-3′ and provided functional evidences that the full length U2 RNA represents the primary precursor of the sRNA populations. Namely, the reported increase in U2-3′ content in transgenic lines over-expressing U2 snRNA robustly supports this possibility. Of particular interest, the expression of both U2-5′ and U2-3′ is strongly repressed in cells grown under iron starvation condition. Interestingly, an induction in the U2 snRNA transcript was observed in the same condition. This negative correlation supports the existence of a regulatory network balancing the reciprocal expression of the U2 snRNA and the U2-derived sRNAs. An increase in the U2 snRNA might be needed to control the cellular responses to deficiency of iron, a critical nutrient for life in the ocean [32, 33, 64, 65]. Likewise, we can also hypothesize a regulatory role played by the U2-derived sRNAs in controlling P. tricornutum’s response to iron availability. Finally, a feedback loop controlling the expression of U2 snRNA precursors by the associated sRNAs might also occur. Interestingly, highly expressed U2-3′ smallRNAs have been detected also in T. pseudonana, strongly supporting a conserved biogenesis of these smallRNA populations in diatoms. The characterization of this complex regulatory process, the identification of U2-3′ mode of action possibly involving sequence-specific targeting, the putative interaction between these sRNAs and the splicing machinery, all represent important questions to address in the future.
Similarly to what was reported in the last years in many organisms [38, 66], we identified a variety of sRNAs associated to tRNAs also in P. tricornutum and T. pseudonana (Additional file 6: Figure S3C and Additional file 7: Table S3). These sRNAs possibly derive from different processing mechanisms and endorse different cellular functions. In P. tricornutum, we found putative tRFs of around 19 nt, mostly corresponding to the tRNA 3′ ends and carrying the trinucleotide CCA at the acceptor stem, which likely derive from tRNA cleavage in or at the T loop . We also detected short fragments associated to the D loop and to the anticodon of 9 different P. tricornutum tRNAs (Additional file 7: Table S3). Because they do not fit into the general classification scheme , we are not able to anticipate the processing events generating these short tRFs from precursor or mature tRNAs. Moreover, we detected several longer small RNAs (30-35 nt) that correspond to both 5′ and 3′ parts of full length tRNAs, and probably derive from cleavages at different positions (Figure 4). Some of these long fragments might be classified as half tRNAs (e.g., GluGAG tRNA). In other species, such tRNA fragments, usually referred to as ‘tRNA, stress-induced small RNAs’, derive from the tRNA cleavage in the anticodon loop, and have been shown to regulate a wide range of cellular processes (e.g., translational efficiency and mitochondrial-mediated apoptosis [48, 66–69] in response to nutritional, biological, or physicochemical stresses). Interestingly, our data also unveil an enrichment of the long tRNA fragments in P. tricornutum cells grown under iron starvation (Additional file 7: Table S3). The data suggest that cells exposed to iron deficiency may differently modulate the processing of specific tRNAs, thereby generating fragments of different sizes that may play different roles in the control of diatom responses to nutrient limitation. We validated the expression of selected sRNAs associated to tRNAs by Northern blot and in agreement with the sequencing data, we observed different populations of tRNA fragments (Figure 4). In general, the detection of the shorter tRFs appeared more problematic in Northern blot analyses as compared to the longer tRFs and to mature tRNAs, likely due to the different stability of these products.
In this study, we also dedicated particular attention to the characterization of sRNAs matching TEs and repeats, which represent almost half of the P. tricornutum small RNA repertoire. TEs are considered an important driving force for genome evolution by inserting at various genomic locations and altering gene expression and regulatory functions [70, 71]. A significant part of diatom genomes is devoted to TEs and repeat sequences, particularly in P. tricornutum. Moreover, a comparative genomic analysis between P. tricornutum and the centric diatom T. pseudonana revealed that TEs have been important contributors to the genome divergence between the two diatom species, with P. tricornutum having undergone a significant amplification of TE sequences as compared to T. pseudonana, respectively 6.4% and 1.9% of the genomes [23, 25]. Interestingly, 7 to 15% of the sRNAs are matching repetitive sequences in T. pseudonana, a proportion significantly lower than in P. tricornutum. These observations suggest that the increased amplification of TEs in P. tricornutum is likely responsible for the over-representation of endogenous sRNAs associated to TEs in this species. Such a mechanism would be consistent with recent findings in Arabidopsis whereby gradual enhanced accumulation of siRNAs derived from the retrotransposon Evadé were also observed upon progressive amplification of this TE in the genome . TE-derived sRNA species likely regulate the expression/transposition of these mutagenic sequences in normal growth conditions but may also control the transposition events under selective conditions thereby contributing to genome evolution and adaptation. Consistently with this concept, expression of some LTR retrotransposons (LTR-RTs) can be modulated by different stresses in P. tricornutum, suggesting that the mobilization of some TEs may contribute to genome rearrangements . The likely essential role of TEs in genome evolution and adaptation, and the high representation of TE-derived sRNAs in P. tricornutum therefore prompted us to explore the possible regulatory role of these sRNA species. Similarly to other organisms, our data clearly indicated that a high sRNA coverage correlates with low TE expression (Figure 5), suggesting that the sRNAs indeed direct TE silencing. We gained more insights into the mechanisms by which sRNAs could direct TE silencing by comparing our small RNA profiling with the methylated regions obtained from a recent genome-wide analysis generated in the same species . This actually confirmed that most of the TEs targeted by sRNAs are characterized by high methylation levels, further supporting the possibility that these ncRNAs contribute to the establishment of DNA methylation at these TEs and to their transcriptional or post-transcriptional silencing. We then extended the analysis of small RNA-associated DNA methylation to coding regions. Interestingly, as already observed by Veluchamy et al., a high methylation level was observed in genes that are specifically covered by sRNAs (Figure 1, Additional file 1: Table S1 and Additional file 8: Figure S4). Our data further show that these sRNAs have sizes and distribution patterns like TE-associated sRNAs, suggesting that they are also generated via similar mechanisms. Interestingly, we already showed in a previous study  that de novo cytosine methylation is associated with transgene silencing induced by artificially expressing antisense and inverted-repeat RNAs in P. tricornutum. Altogether, this information suggests that a small RNA-dependent DNA methylation process is widely used by diatoms not only to control the expression of TEs but also of transgenes as well as protein-coding genes by presumably directing DNA methylation within these genetic elements. These results also open interesting novel questions on the evolution, distribution and function of such epigenetic-based regulatory pathways in different organisms. In mammals, DNA methylation occurs throughout the genome, and many genes are body methylated, although this methylation pattern has not been associated with the presence of sRNAs . In wild-type Arabidopsis plants, gene body methylation has also been reported for many genes, but this methylation occurs in a small RNA-independent manner and appears to be irrelevant for silencing . By contrast, loss-of-function mutations in some active Arabidopsis DNA demethylase mutants were reported to enhance DNA methylation in specific gene body regions through siRNA-mediated DNA methylation, presumably leading to their silencing [75, 76] and supporting a functional role for siRNA-directed gene body methylation in these mutant backgrounds. Therefore, we can hypothesize that different photosynthetic organisms belonging to different lineages had the potential to use the small RNA-dependent DNA methylation pathway to control expression of coding genes, although in plants this effect is solely appreciated in specific mutants but not in wild-type plants grown under standard conditions. Furthermore, the sRNA distribution and DNA methylation profile over gene bodies differs significantly between diatoms and plants (Figure 1 and Additional file 8: Figure S4) . Therefore, the observations made on P. tricornutum will provide fertile ground for new exciting insights into the mechanisms involved in small RNA-directed gene body methylation and into the eventual effects of such epigenetic modification on transcription initiation and elongation.
In addition to the likely functional relevance of P. tricornutum TE-associated sRNAs in DNA methylation and transcriptional gene silencing, we found that these sRNA species were 25 to 30 nt in length and have a 5′ uridine bias (Figure 5). Interestingly, these features are shared with animal piRNAs suggesting a potential link between these silencing pathways. However, our analysis did not reveal self-complementary sequences, characteristic of a Ping-Pong amplification, and no canonical PIWI protein could be retrieved in the P. tricornutum genome (data not shown), arguing against the presence of a canonical piRNA biosynthetic pathway in this marine microalga. Future in depth characterization of such diatom small RNA species will thus be necessary to compare and contrast these sRNAs with animal piRNAs.
The periodic distribution of sRNAs over methylated regions represents a completely novel hint. This phenomenon is characterized by a 180 nt-long period (at times of 90 nt) that is sharp on coding genes and also visible with high confidence on TEs. At this stage, it is still difficult to predict the mechanisms generating such a peculiar distribution pattern or to link sRNA distribution with DNA methylation activities and transcriptional silencing. In future studies, it will be relevant to assess whether periodic sRNA distribution correlates with local chromatin properties such as nucleosome occupancy, histone post-translational modifications or histone variants, which have not been mapped in diatoms so far. More specifically, it might be interesting to test whether the 180 nt periodic enrichment of sRNAs matches inter-nucleosomal domains with about the same size, possibly reflecting the capacity of RNA Polymerases producing small RNAs to efficiently transcribe these more accessible regions in a condensed and silenced chromatin context. Reciprocally, on protein-coding genes, sRNAs might also target inter-nucleosomal domains more easily than histone-wrapped nucleosomal DNA, particularly in a non-replicative context. Interestingly, the existence of periodic cytosine methylation has recently been detected in microalgae with compact nuclei and strong gene body methylation, regardless of gene expression levels. In Ostreococcus lucimarinus and Micromonas pusilla, the periodic DNA methylation occurs specifically over nucleosome linker (inter-nucleosomal) regions, and appears to contribute to genome architecture and nucleosome positioning . Based on our observations, it might be interesting to test whether sRNAs also display a periodic enrichment at the same loci in these species. Of note, regularly spaced methylation has not been detected in P. tricornutum. This might reflect the different methylome features between P. tricornutum and these other algae. Alternatively, more refined DNA methylation mapping might be required to determine whether periodic DNA methylation and sRNA distribution overlap at some TEs or gene loci in P. tricornutum.
The analysis of diatom genomes reveals the presence of genes encoding several proteins that might be required for generating DNA methylation-associated sRNAs. P. tricornutum and T. pseudonana genomes both encode Dicer- and Argonaute-like proteins and several putative RNA-Dependent RNA Polymerases (RdRPs) but no PIWI protein . These proteins might be responsible for generating sRNAs, for amplification mechanisms and for the possible recruitment of DNA methyltransferases (DNMTs) identified in previous studies [25, 77]. Considering the peculiar genome of diatoms, their evolutionary history, and the information on the sRNAs landscape from this study, we also expect that additional, still unknown, components participate in the small RNA-based regulation of diatom genome function. Their characterization will likely provide novel insights into the genetic bases allowing the successful proliferation of diatoms in the oceans and will help addressing relevant questions about the conservation of these processes in organisms that cover different branches of eukaryotic evolution.
This comprehensive characterization of the P. tricornutum small RNAs landscape uncovered an unexpectedly complex composition of the sRNA complement in diatoms. While miRNAs seem not to play a major regulatory role in P. tricornutum, other ncRNA types with well-described function, such as tRNAs and U2 snRNA, represent a major source of sRNAs and could play important functional roles in P. tricornutum’s responses to environmental stresses such as iron starvation. This discovery also implies that complex and interconnected networks of ncRNA pathways exist in diatom cells, and their fine-tuning is likely to be fundamental for the biology of these organisms and their extraordinary ability to proliferate worldwide. The existence of highly expressed sRNAs of unknown origin and conserved in two distantly related diatom species also indicates that diverse small RNAs sources await to be discovered in these algae. The most represented population of sRNAs match TEs and likely contributes to their transcriptional silencing through a yet-to-be characterized RNA-dependent DNA methylation mechanism. Protein-coding genes might also be subjected to similar regulatory processes for down regulating their expression at a transcriptional, and possibly also at a post-transcriptional level. In conclusion, this extensive characterization of P. tricornutum small ncRNAs can contribute to future studies aiming at understanding diatom biology, the molecular bases underlying their ecological success in the contemporary oceans, and their capacity to optimally perform in highly changing environments. The periodic pattern of sRNAs distribution associated with genic DNA methylation unveiled here, represents an unexpected discovery that opens the way to search for similar periodic features in other eukaryotic groups.
Cell cultures conditions
The CCMP632 strain of P. tricornutum Bohlin was obtained from the Provasoli-Guillard National Center for Culture of Marine Phytoplankton. Cultures were grown in f/2 medium  at 18°C under white fluorescent lights in 12 h light: 12 dark cycles. For the preparation of the different small RNA libraries, cells were grown under different conditions: Low Light (LL) (30 μmol photons m−2 s−1), Normal Light (NL) (80 μmol photons m−2 s−1); in dark for 60 hours (D) and then shifted to High Light (500 μmol photons m−2 s−1) for 5 hours (HL) and under iron starvation (iron 5nM). For the iron starvation, cultures of P. tricornutum were grown at low light, in Artificial Sea Water medium , containing 5 nmol · liters−1 Fe (Fe-limited) as final concentration. In all the experiments, cells in the exponential phase of growth have been used.
Small RNA library preparation and sequencing
In all conditions (NL, HL, LL, D and iron starvation), total RNA was extracted from diatom cells using the Trizol method (Invitrogen, USA). sRNAs were separated by size fractionation on denaturing polyacrylamide gels and then fragments in the 18–35 nt size range were purified. The RNA samples were sent for cDNA synthesis and Illumina sequencing to Vertis Biotechnologie AG company (http://www.vertis-biotech.com). The purified RNAs were poly(A)-tailed and ligated using a RNA adapter to the 5′-phosphate of the small RNAs. First-strand cDNA synthesis was performed using an oligo(dT)-adapter primer and M-MLV RT (Ambion), then followed by PCR amplification. The resulting cDNA populations were sequenced on an Illumina sequencer. The raw sequence data can be accessed at the NCBI Sequence Read Archive under the accession number SRX648639.
Read mapping and primary data analyses
The reads coming from the 5 libraries associated to different environmental conditions were aligned to the P. tricornutum genome with MicroRazerS  and subsequently quality filtered (Additional file 5: Supplementary Methods). Read coverage was computed in two ways, either by considering only unique matches, or by reweighting each read according to the number of matching locations. Unless stated otherwise, we considered unique matches in our analyses. All data analyses were done using a combination of python, R and shell scripts, and genome arithmetic tools [80, 81].
Using as genome reference Phatr v2.0 from JGI , gene annotation was derived from EST expression data (libraries os, sp, sm and om referenced in ), and non-coding RNA annotation from RFam v11.0 . Additionally, tRNA genes were all reannotated using tRNAscan . RNA-Seq expression levels and methylation data were obtained from . Due to the large dynamic range gained by the RNA-Seq, we define a category of lowly expressed genes at the 15th percentile of all genes expression. Within a genomic interval, the methylation level is reported as the proportion of probes called as methylated on that interval.
The list of autonomous transposable elements was derived from  and realigned using LAST , resulting in the identification of 119 autonomous regions on the genome. The transcription level of an autonomous transposon (EST or RNA-Seq evidence) was deduced from the expression level of the gene models overlapping it, taking the highest value.
Prediction of RNA secondary structures was done with RNAfold  and visualization with Varna . To account for the natural diatom environment, we also lowered the default folding temperature to 24°C.
Prediction of processed elements
miRNA-like elements were predicted on a set of regions obtained by clustering reads that aligned less than 100 bp from each other, and covered by at least 10 reads (called sRNA producing loci).
Then loci, where the aligned reads profile is compatible with bona fide small ncRNA processing, were selected according to a set of published and empirical criteria [34, 35]. All the filtering steps are described in details in Additional file 5: Supplementary methods. In summary, we selected loci with at least one strand-specific, highly localized fragment in the 19-29 nt range in a fashion like what is classically used for prediction in plants and animals high-throughput sequencing data [87–89]. The set of identified loci is then merged across the five libraries by taking the union of all genomic intervals, resulting in 50 candidate loci.
When analyzing a previously published dataset of small RNA sequences , SRA: GSM72456[0–2], we used the same strategy for sequence alignment and annotation of the candidate regions. The small RNA sequences coming from an experiment on T. pseudonana (SRA027146, SRA027168) were also aligned with the same strategy on the reference genome. We predicted miRNAs with MIReNA v2.0 , recently shown to perform amongst the best methods for de novo miRNA annotation . We used our list of candidate regions as putative precursors –excluding known non-coding RNAs. The 20 putative precursors are obtained by extending the candidate region by 50 nt on both sides, and the putative mature fragments are proposed amongst the 6 more abundant in the region. We additionally evaluated the significance of the MFE m associated with the predicted fold by counting the proportion of samples with an MFE lower than m over 1000 random regions of the same size drawn from P. tricornutum genome. The derived empirical pvalue is then deemed significant when below 5%.
Analysis of the periodic distribution of reads along the P. tricornutumgenome
To detect and quantify periodic distribution of read coverage, we performed analysis based on Fast Fourier Transform. Regions with periodic distribution are detected by sliding a window of length 1000 bp (100 bp increments) along each chromosome. In each window, a Fast Fourier Transform (FFT) is computed based on the profile of read coverage and including multimapping reads. The main period is extracted together with the corresponding FFT coefficient, normalized by the total signal. Windows with a coefficient higher than 75% of the maximal value are annotated with a significant period.
Northern blot analysis of low molecular weight RNAs
Total RNA was isolated as described above. Northern blot analyses of low molecular weight RNAs were performed by separating 20 μg of total RNA on 14% acrylamide gels. RNAs were transferred to N+ membranes (Amersham Bioscience) by using a semi-dry system (BioRad). For the detection of small RNAs, membranes were hybridized overnight with oligonucleotides complementary to the small RNA sequences. The probes were end labeled with γ-32P-ATP using T4 PNK (New England Biolabs, Beverly, MA) in PerfectHyb Plus hybridization buffer (Sigma). Different temperatures were used for the hybridization according to the different Tm of the probes. The membranes were washed twice for 20 min with 2× SSC, 1% SDS at 40°C, and then scanned on a Typhoon Imaging System 9200 (GE Healthcare) or exposed to x-ray films.
Analysis of sRNAs by stem-loop qPCR
The expression profiling of selected sRNAs was also analyzed with the Stem-loop Real-time reverse transcriptase polymerase chain reaction (RT-PCR) . In brief, 2 μg total RNA were reverse transcribed with specific RT-primers, and the resulting cDNA was subjected to real-time reverse transcription quantitative qRT-PCR with a SYBR Green I assay (Roche SYBR Green I Master), using specific sRNA forward primers and the universal reverse primer. The primers were designed according to published criteria [90, 91] and are specified in Additional file 11: Table S4. The small nucleolar RNA SnoR85 was used as endogenous reference. In our analyses, quantification of each small RNA relative to snoRNA was calculated using the following equation: N = 2−ΔCt, ΔCt = CtsmallRNA − CtsnoRNA. All reactions were carried out in triplicate.
Characterization of the full length snRNA U2 by 3′ and 5′ RACE-PCR
The sequence of the snRNA U2 full-length transcript was determined by FirstChoice® RLM-RACE Kit (Invitrogen™). cDNA was amplified in accordance with the manufacturer’s instructions. The primers used in the 5′ and 3′ RACE PCR are specified in Additional file 11: Table S4. RACE products were cloned into TOPO® PCR vector (Life Technologies) and sequenced.
Expression analysis of snRNA U2 and TEs
RNA extraction and quantitative PCR (qRT-PCR) were performed as described in De Riso et al. . The cDNA was quantified using a SYBR Green qPCR kit (Roche LightCycler 480 SYBR Green I Master) and gene specific primers. The RPS housekeeping gene (ribosomal protein small subunit 30S; Protein ID 10847) or the histone H4, were used as reference genes (Additional file 11: Table S4). All reactions were carried out in triplicate.
Construction of vectors for U2 sRNA overexpression
The vector for the U2 snRNA overexpression was generated using standard molecular cloning procedures. The full length U2 snRNA was amplified by PCR from the Pt1 cDNA using the following primers OE U2snRNAFw (XbaI site in italic): ctagTCTAGATTCGCCTTATTGGCTTTGAT and OE U2snRNARv (EcoRI site in italic): ccgGAATTCgagggaAAAGTGGGGGTACA. The fragment was cloned downstream a phleomycin resistance cassette (Sh ble gene), and under the strong FcpF promoter, as described in .
The vector was introduced into wild-type P. tricornutum strain by microparticle bombardment using a BiolisticPDS-1000/He Particle Delivery System (Bio-Rad)  and transgenic lines were selected on 1% agar plates (50% f/2 medium) containing 100 μg/mL Phleomycin (Invivogen) in Normal Light (80 μmol photons m−2 · s−1). The presence of the construct in the transgenic lines was verified by checking the presence of the Shble gene by PCR with the primers Shble1fw and Shble1 rv. The presence of the full length U2 snRNA was verified by performing PCR using the primers Shble1 fw and snRNAU2 rv (Additional file 11: Table S4). On the selected transgenic lines, Northern blot analysis was performed, as described before. For the detection of the sRNAs, the membrane was hybridized overnight with oligonucleotides complementary to the U2-3′ sequence.
Availability of supporting data
The raw sequence datasets supporting the results of this article are available in the sequence read archive repository (http://www.ncbi.nlm.nih.gov/sra/) under the accession number SRX648639. Additional supporting methods, figures and tables are included as supplementary files.
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We thank Claudia Steglich (University of Freiburg, Germany) and Odon Thiébeauld (IBENS Paris, France) for inputs for the small RNAs experimental analyses, Anthony Mathelier for first insights into the MIReNA software, the Chris Bowler’s team (IBENS Paris, France) for sharing RNA-Seq and methylation data, Marianne Jaubert for critical reading of the manuscript. This work was supported by the Action Thématique et Incitative sur Programme [ATIP CNRS Fr, 2009 to A.F.]; Japan Society for the Promotion of Science [PE11014 to H.R.]; Institut Universitaire de France to A.C.; People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme 2007–2013 FP7 [317184 to B.V. and W.R.H].
The authors declare that they have no competing interests.
AF, AR, HR, WRH and AC conceived the experiments. Experiments were conducted by AR and SCN and partly in the LN lab. Data analyses and primer design were conducted by HR, with the help from AS, BV, RC. The manuscript was written by AF, AR, HR, LN and AC, and was read, edited and approved by all the authors. All authors read and approved the final manuscript.
Alessandra Rogato, Hugues Richard contributed equally to this work.
Electronic supplementary material
Additional file 2: Table S2: List and properties of regions annotated with our set of filters for miRNAs identification. (PDF 44 KB)
Additional file 3: Figure S1: Predicted secondary structures and read profiles for a miRNA-like candidate on chr10 predicted by MIReNA. (PDF 295 KB)
Additional file 4: Figure S2: Predicted secondary structures and read profiles for a miRNA-like candidate on chr1 predicted by MIReNA. (PDF 292 KB)
Additional file 9: Figure S5: Distribution of the main period in sRNA coverage on the set of HMR regions longer than 1000 bp (351 regions). (PDF 41 KB)
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Rogato, A., Richard, H., Sarazin, A. et al. The diversity of small non-coding RNAs in the diatom Phaeodactylum tricornutum. BMC Genomics 15, 698 (2014). https://doi.org/10.1186/1471-2164-15-698