Role of oxygen and the OxyR protein in the response to iron limitation in Rhodobacter sphaeroides
© Remes et al.; licensee BioMed Central Ltd. 2014
Received: 25 April 2014
Accepted: 8 September 2014
Published: 15 September 2014
High intracellular levels of unbound iron can contribute to the production of reactive oxygen species (ROS) via the Fenton reaction, while depletion of iron limits the availability of iron-containing proteins, some of which have important functions in defence against oxidative stress. Vice versa increased ROS levels lead to the damage of proteins with iron sulphur centres. Thus, organisms have to coordinate and balance their responses to oxidative stress and iron availability. Our knowledge of the molecular mechanisms underlying the co-regulation of these responses remains limited. To discriminate between a direct cellular response to iron limitation and indirect responses, which are the consequence of increased levels of ROS, we compared the response of the α-proteobacterium Rhodobacter sphaeroides to iron limitation in the presence or absence of oxygen.
One third of all genes with altered expression under iron limitation showed a response that was independent of oxygen availability. The other iron-regulated genes showed different responses in oxic or anoxic conditions and were grouped into six clusters based on the different expression profiles. For two of these clusters, induction in response to iron limitation under oxic conditions was dependent on the OxyR regulatory protein. An OxyR mutant showed increased ROS production and impaired growth under iron limitation.
Some R. sphaeroides genes respond to iron limitation irrespective of oxygen availability. These genes therefore reflect a “core iron response” that is independent of potential ROS production under oxic, iron-limiting conditions. However, the regulation of most of the iron-responsive genes was biased by oxygen availability. Most strikingly, the OxyR-dependent activation of a subset of genes upon iron limitation under oxic conditions, including many genes with a role in iron metabolism, revealed that elevated ROS levels were an important trigger for this response. OxyR thus provides a regulatory link between the responses to oxidative stress and to iron limitation in R. sphaeroides.
KeywordsRhodobacter sphaeroides Transcriptomics Iron limitation OxyR RNAseq Oxidative stress
All aerobic bacteria produce toxic oxygen derivatives as by-products of their metabolism. Therefore, they have evolved complex defence and repair mechanisms to protect themselves from the damaging effects of emerging reactive oxygen species (ROS) . These mechanisms allow bacteria to cope with oxidative stress and the imbalance between ROS-generating and ROS-defence processes [2, 3]. A direct detoxification of ROS is catalysed by such enzymes as superoxide dismutases (SOD), catalases and peroxidases [4, 5]. Surprisingly, strictly anaerobic bacteria express comparable detoxifying systems, such as the SOD, but little is known about their role and the possibility of ROS protection in these organisms [1, 6].
As a cofactor of several enzymes and regulatory proteins, iron is an essential element for living organisms. Iron is mainly present in two forms, either as soluble ferrous iron Fe(II) or as insoluble ferric iron Fe(III). In oxic environments, only Fe(III) is energetically stable , while in anoxic habitats, reduction to Fe(II) occurs chemically by organic compounds, such as sulphides . Bacteria possess numerous highly efficient iron acquisition systems to scavenge iron from the environment under iron-restricted conditions, such as the synthesis and secretion of high affinity extracellular ferric chelators called siderophores [9, 10]. However, siderophores can have physiological roles aside from those involved in iron acquisition; one such example is siderophores acting as protectors against oxidative stress . Although iron is essential for bacteria, iron potentiates oxygen toxicity by the production of hydroxyl radicals via the Fenton reaction. Fenton-like reactions are not restricted to iron as the reactive metal component. Other metals, such as copper, which is required for many cellular enzymes, such as cytochrome oxidase or SOD [12, 13], also catalyse the generation of reactive hydroxyl radicals that cause cellular damage . On the other hand, iron is required for some enzymes involved in ROS detoxification (e.g., catalases and Fe-containing SOD) or in sensing oxidative stress (e.g., SoxRS). Thus, iron limitation interferes with the oxidative stress response. Iron limitation in photosynthetic organisms, such as Anabaena sp. Strain PCC 7120, Synechocystis sp. Strain PCC 6803 or R. sphaeroides, resulted in a 2- to 10-fold increase in ROS levels compared with those found in cells grown with appropriate iron supplementation [15, 16]. However, when non-photosynthetic Escherichia coli or Bacillus subtilis cells were iron starved, they did not exhibit a significant increase in the ROS levels . Therefore, Latifi et al. proposed in 2005 that oxidative damage induced by iron starvation could be a characteristic of photosynthetic organisms. However, life in the presence of oxygen requires a strict regulation of iron metabolism in all organisms.
The oxidative stress response in E. coli mainly relies on the genes of the OxyR and SoxRS regulons . A Salmonella oxyR deletion mutant was discovered to be hypersensitive to hydrogen peroxide (H2O2) . Furthermore, the OxyR protein positively regulates the expression of the ferric uptake regulator fur gene in response to H2O2 . In E. coli and in many other bacteria, the Fur protein is the main regulator of iron-dependent gene expression (reviewed in e.g., ). This pattern of gene expression constitutes a regulatory link between oxidative stress responses and iron homeostasis. Due to increased Fur levels during H2O2 stress, Fe(II) binding and iron storage is induced, leading to reduced free iron levels, which in turn help bacteria to cope with oxidative stress .
Experimental data and bioinformatic analyses suggest that in α-proteobacteria, iron regulation mainly occurs via regulators other than Fur , and nothing is known regarding the regulatory link between iron metabolism and defence against oxidative stress. R. sphaeroides is a facultative photosynthetic bacterium, which performs aerobic respiration in the presence of oxygen. In anoxic conditions in the light, anoxygenic photosynthesis generates ATP, while in the dark and in the presence of an electron acceptor, such as dimethyl sulphoxide (DMSO), anaerobic respiration can be performed. An earlier transcriptome study revealed that many of the genes involved in iron metabolism are induced in response to H2O2, demonstrating a strong correlation between oxidative stress responses and iron metabolism in this bacterium . Several of these regulated genes are controlled by the intensively studied OxyR regulator . Transcriptome studies also identified genes affected by iron limitation in R. sphaeroides and revealed that the Fur-related proteins Fur/Mur and Irr have no major function in activating gene expression in response to iron limitation . The goal of this study is to discriminate between direct effects of iron limitation on gene expression and indirect effects, which are caused by the oxidative stress that occurs due to iron depletion . Towards this end, the effects of iron limitation on global gene expression in anoxic conditions and oxic conditions were compared. Although there was a strong correlation between the two data sets, a number of genes exclusively responded to iron-limiting conditions in the presence of oxygen, while others responded only under anoxic conditions. Furthermore, we provide evidence for an important role of OxyR in iron-dependent activation of genes under oxic growth conditions.
Results and discussion
A subset of genes responds to iron limitation independently of oxygen availability
Only few global gene expression studies of iron limitation performed in oxic conditions simultaneously examined gene expression under iron-limiting, anoxic conditions (e.g., ). In order to discriminate the effects on gene expression that are a direct consequence of iron limitation from those caused by increased ROS levels due to iron limitation , we analysed R. sphaeroides transcriptomes in both oxic (25–30 μM oxygen, low oxygen tension) and anoxic conditions (using DMSO as terminal electron acceptor). To generate iron limitation, cultures were grown without external iron in the presence of the iron chelator 2,2′-dipyridyl (30 μM). The high metabolic versatility of R. sphaeroides allows such comparative studies, as it is capable to grow by both aerobic respiration and anaerobic respiration (if an alternative electron acceptor is available).
Quantified log 2 fold changes in response to iron-limitation
fliC, flagellar protein
mbfA, ferritin-like protein
ccpA, cytochrome c peroxidase
puc2A, light-harvesting complex
bchY, photopigment biosynthesis
bchN, photopigment biosynthesis
bchL, photopigment biosynthesis
hemC, Vitamin B12 synthesis
appA, blue light sensor
exbB, biopolymer transport protein
exbD, biopolymer transport protein
tonB, iron transporter
ABC ferric transporter
bfd, bacterioferritin associated ferredoxin
irpA, iron-regulated protein
afuA, ABC siderophore transporter
hemP, hemin uptake protein
metI, methionine uptake transporter
cysK, cysteine synthase
feoA1, ferrous iron transport protein
cysA, ABC sulfate transporter
cysP, ABC sulfate transporter
feoA2, ferrous iron transport protein
phaD, NADH dehydrogenase
znuB, ABC zinc transporter
sufD, iron-regulated ABC transporter
sufC, iron-regulated ABC transporter
sufB, iron-regulated ABC transporter
iscR, iron sulfur cluster regulator
sitC, ABC Mn2+ transporter
znuC, ABC zinc transporter
rpoH II , RNA polymerase sigma factor
rpoE, sigma factor
Genes with oxygen-dependent responses to iron limitation show different distinct expression patterns
For the majority of the 1318 iron-regulated protein-coding genes (945 genes, red spots in Figure 1A), the response to iron was dependent on oxygen availability. Because these genes showed distinct expression patterns, we assigned them to different clusters for further analysis and discussion. Figure 1B depicts these different clusters of genes, omitting all genes that are not regulated by iron or that show an oxygen-independent response to iron limitation (grey and green spots in Figure 1A).
One hundred eighty-four genes with a stronger down-regulation in response to iron limitation in oxic conditions than in anoxic conditions are grouped in cluster I (Figure 1B, purple spots). Most of these genes have a function in chemotaxis and motility (che, mot, flg, flh, fli). It was shown previously that these genes are also down-regulated in response to oxidative stress in R. sphaeroides , indicating that these genes are likely repressed by ROS in particular. An effect of iron on the expression of motility genes was also observed in Helicobacter pylori , Vibrio cholerae  and Pseudomonas fluorescens . Cluster I includes also most genes for the synthesis of pigment-binding proteins (pufBALMX, pucAB, puc2BA). Several genes of the nuo operon encoding subunits of the NADH dehydrogenase complex are also part of cluster I. This enzymatic complex comprises iron sulphur clusters. Repression under oxic conditions when the amount of iron cofactor is limited therefore reduces the production of these proteins and may also reduce the production of further ROS. Under anoxic conditions, the expression of nuo genes is very low, and no additional repression by iron limitation is necessary. The RNAseq data for these experiments are publicly available through the NCBI’s GEO database (accession number GSE47182).
Cluster II (Figure 1B, orange spots) consists of 330 genes that show an expression change in response to iron limitation of log2 < -1 in oxic conditions and log2 > 0 in anoxic conditions. Most of these genes function in the synthesis of bacteriochlorophyll (e.g., bchE, bchC, bchJ, bchY), the synthesis of heme and bacteriochlorophyll precursors (hemN, hemC, hemZ, hemB, hemA), or the synthesis of carotenoids (crtF, crtE, crtC, crtA). Because bacteriochlorophyll synthesis and energy conversion in the reaction centre require iron [32, 33], a repression of genes that function in photosynthesis in response to iron limitation is meaningful. However, the exclusive repression under oxic conditions indicates that the repression is a response to the elevated ROS levels that occur due to iron limitation. This result is in agreement with the repressing effect of oxidative stress and photooxidative stress on these genes [20, 28].
Cluster III includes 51 genes (Figure 1B, red spots) that exhibit stronger upregulation in response to iron limitation in oxic conditions than in anoxic conditions. Many genes with predicted functions in ferric iron uptake and storage are among this cluster, including exbBD and tonB, RSP_1438-1440 encoding a ferrichrome transporter, bfd encoding a bacterioferritin, irpA encoding an iron-regulated protein, genes encoding Fe3+-siderophore transporters and hemP encoding an iron uptake protein. An induction of genes for ferric iron uptake and transport under iron-limitation conditions helps to counteract the iron limitation. Under anoxic conditions, iron is mostly present as ferrous iron, and a strong upregulation of these genes would not be appropriate.
Cluster IV comprises 160 genes (Figure 1B, blue spots) that are exclusively or much more strongly upregulated in anoxic iron-limiting conditions. Among these genes, we found the ferrous transport system feoAB, several genes involved in cysteine and sulphur metabolism, including RSP_1944-1939 (sulphate reduction) and cysK, cysAPTW, and metNIQ (methionine uptake) and genes with other functions, such as RSP_3323 encoding a flavoprotein, RSP_2890-2891 encoding a copper transport protein and CueR, a regulator of RSP_2890-2891 . An upregulation of the ferrous transport system feoAB and other metal transport systems would help to counteract iron limitation under anoxic conditions.
Cluster V includes 164 genes (Figure 1B, black spots) with an exclusive or much stronger repression in response to iron limitation under anoxic conditions compared with oxic conditions, and these genes have very diverse functions. Two of the genes, groES and groEL, have a role in the heat shock response, 13 of the genes encode ribosomal proteins, 20 of the genes encode transporters and 4 of the genes encode putative transcriptional regulators. In addition, genes for hydrogenase production and regulation (hup, hyp) fall into this cluster. The iron-containing hydrogenases might be non-essential, and their down-regulation upon iron limitation would therefore free up trace amounts of iron for more crucial proteins. Genes for hydrogenase production and regulation are only weakly expressed in oxic conditions, and no further repression under iron-limitation conditions is required (GEO series GSE47182).
Remarkably, 56 genes (Figure 1B, green spots) grouped in cluster VI showed opposite responses to iron limitation in the presence or in the absence of oxygen. Under iron depletion, most genes of the isc-suf operon for iron-sulphur cluster assembly were induced in an oxic environment but repressed under anoxic conditions. An increase of the Suf machinery to enable iron-sulphur cluster assembly under low iron concentrations is understandable. The demand for repair of damaged iron-sulphur clusters is higher under oxic conditions, as elevated ROS levels destabilise Fe-S clusters. Hence, it is not surprising that these genes were also upregulated in the presence of H2O2 stress [20, 28]. In anoxic conditions, Fe-S clusters remain stable; consequently, the isc-suf operon is repressed. However, several metal transporters, such as modA and modD for molybdate transport or RSP_2890-2891 for copper transport, are exclusively induced in anoxic conditions, while genes coding for sulphate/thiosulphate transporters (cysAPTW) or methionine uptake transporters (metNIQ) are more strongly induced compared with oxic conditions. We presume that in iron-limiting anoxic conditions, a change in the demand for cofactors takes place and iron is partly replaced by other metals.
Role of the OxyR protein in the response of R. sphaeroidesto iron limitation in oxic and anoxic conditions
A response of many iron homeostasis genes, including sufBCD, bfd-bfr or exbBD-tonB, to H2O2 exposure in R. sphaeroides was previously observed, and an influence of the OxyR protein was revealed for most of these genes [20, 21, 28]. To elucidate the role of OxyR in iron-starved cells, we compared log2 ratios in response to iron limitation using real-time RT-PCR for the wild type strain and a strain lacking OxyR (2.4.1ΔoxyR) due to a chromosomal deletion . We selected those genes that showed a strong regulation in the RNAseq or microarray analyses (Additional file 1: Table S1). Genes from all six clusters were selected to test for a potential cluster specificity of OxyR.
The stronger induction in response to iron starvation in oxic conditions of cluster III genes, namely exbBD, tonB, bfd, irpA, afuA, hemP and RSP_1438 (ABC ferric transporter), revealed by RNAseq analysis was confirmed by real-time RT-PCR (Table 1). However, the induction in oxic conditions was significantly reduced in the oxyR deletion strain, while the oxyR deletion had no significant effect under anoxic conditions. A similar expression pattern was observed for cluster VI genes, represented by genes of the isc-suf operon, the sitABCD operon (ABC Mn2+/Fe2+ transport system ) and znuC (ABC zinc transporter) (Table 1). Induction of these genes in response to iron limitation under oxic conditions was significantly dependent on OxyR.
OxyR-dependent upregulation of cluster III and cluster VI genes may prevent a stronger oxidative stress; e.g., by removing free iron (bacterioferritin), removing oxygen (cytochrome c oxidase) or upregulating the oxidative stress response (RpoE). Our data provide the first clue regarding a regulatory link between iron metabolism and oxidative stress defence in an alpha-proteobacterium: OxyR, which is activated by oxidative stress and consequently affects the expression of genes involved in the oxidative stress response, also activates genes important for iron metabolism in oxic conditions with iron limitation.
The fact that most cluster III genes showed some activation under oxic iron-limiting conditions in the oxyR mutant implies that factors other than OxyR are responsible for maximal induction. These factors may also trigger the observed activation of cluster III genes under anoxic conditions, which was independent of OxyR.
While all of the tested genes for ferric iron transporter systems (cluster III genes) were regulated in an OxyR-dependent manner, the ferrous transport system feoAB and other cluster IV genes were not influenced by OxyR (Table 1). The same result holds true for the remaining clusters I, II and V. The only exception was the groEL operon (cluster V); however, the cluster V expression pattern was not confirmed by real-time RT-PCR analysis.
The possibility that the expression discrepancies in the background of ∆oxyR between oxic and anoxic conditions were DMSO dependent can be excluded. We compared transcript level of wild type and ∆oxyR cultures grown in oxic conditions in presence or absence of DMSO. No significant changes could be observed due to DMSO addition (p-value ≤0.05) (Additional file 3: Figure S1).
In a previous study, we reported that a consensus sequence for the DNA binding site of OxyR does not exist in Rhodobacter . Only the pattern TN11A, defining the minimum binding site for LysR-type regulators, was present in the majority of OxyR binding regions. In an effort to obtain more detailed information about the promoter specificity of the genes in the six clusters, we used the MEME program (http://meme.sdsc.edu./meme/) to identify conserved, overrepresented DNA motifs in the upstream promoter region of the selected genes . As the search did not reveal clear DNA motifs for an individual cluster, we assume that several transcription factors are responsible for the differential expression or that the consensus for the binding sequence is very weak, as in the case of OxyR.
Iron starvation impairs growth of strain 2.4.1oxyRin oxic, but not in anoxic, conditions
Doubling times of wild type and 2.4.1∆ oxyR
Doubling times (h)
3.9 ± 0.2
12.5 ± 1.2
Wild type -Fe
4.3 ± 0.3
16.1 ± 1.5
4.2 ± 0.2
22.4 ± 2.9
4.3 ± 0.1
21.8 ± 2.2
Our data suggest that the elevated ROS levels under iron limitation in oxic conditions (Figure 2B) increased the level of oxidised OxyR and consequently led to the activation of OxyR-dependent genes. The increased katE level under oxic iron limiting conditions (log2 fold change 1.2, Table 1) is in agreement with this assumption. katE activation under oxidative stress strictly depends on OxyR.
Furthermore, a strain lacking the oxyR gene showed significantly reduced doubling times and a lower end-OD than the parental strain in anoxic conditions irrespective of iron availability (Table 2, Additional file 3: Figure S2). This result is in agreement with our transcript level analysis, where OxyR has nearly no notable influence on the anoxic response to iron limitation (Table 1). The possibility that the growth phenotypes were due to toxicity of the alternative electron acceptor (DMSO) can be excluded, as cultures grown in oxic conditions with DMSO did not exhibit impaired growth (data not shown). Up to now, we cannot ascribe a role to OxyR in an anoxic environment. Nevertheless, our observations indicate an important role for OxyR, even in the absence of molecular oxygen or ROS.
Iron limitation increases ROS levels in R. sphaeroides ( and this study). To discriminate the effects on gene expression that are a direct consequence of iron limitation from the effects caused by the increased ROS levels under iron limitation, we compared gene expression under iron limiting conditions in the presence and absence of oxygen in R. sphaeroides. Of all the genes responding to iron limitation, almost one third showed the same response in the presence and absence of oxygen, implying that these genes exhibited an iron-specific response. Apparently, a link between iron availability and oxidative stress exists only for a subset of iron-dependently regulated genes.
The other two thirds of the iron-regulated genes showed diverse expression patterns that were influenced by oxygen availability, and the known regulator OxyR was identified as an important factor for induction of many genes involved in iron metabolism under oxic conditions. For those genes, elevated ROS levels were the trigger of the response rather than iron limitation itself. The OxyR regulator provides a link between the responses to oxidative stress and iron. In the future, additional regulators that are responsible for OxyR-independent activation in oxic conditions, activation in anoxic conditions and repression in response to iron limitation will need to be identified.
Bacterial strains and growth conditions
The strains and plasmids used in this study are listed in Additional file 4: Table S3. E. coli strains were grown in Luria–Bertani medium at 37°C with shaking (180 rpm) or on solid growth medium, which contained 1.6% (w/v) agar. R. sphaeroides strains were cultivated at 32°C in 50-ml Erlenmeyer flasks containing 40 ml malate minimal medium (Additional file 4: Table S4) with continuous shaking at 140 rpm, resulting in a constant dissolved oxygen concentration of approximately 25–30 μM during the exponential phase. These growth conditions are designated as oxic growth. To achieve anoxic conditions, we used completely filled screw-cap Meplat bottles for liquid cultures, which were sealed with Parafilm and cultivated in the dark. The remaining oxygen was used up by the cultures within 60 seconds, as confirmed using an oxygen sensor. To allow anaerobic respiration, dimethyl sulphoxide (DMSO) was added as electron acceptor at a final concentration of 60 mM. Anoxic incubation over several days resulted in a final OD660 of approximately 0.5. Conditions of iron limitation were achieved by transferring R. sphaeroides into iron-limited malate minimal medium containing the iron chelator 2,2′-dipyridyl (30 μM; Merck KGaA) three times. Inductively coupled plasma mass spectrometry (ICP-MS) using an Agilent 7500ce spectrometer confirmed that the iron content was drastically reduced in iron-limited medium (from 140 mg l-1 to 16 mg l-1) . When required, antibiotics were added to liquid or solid growth media at the following concentrations: spectinomycin (10 μg ml-1); kanamycin (25 μg ml-1); tetracycline (2 μg ml-1) (for R. sphaeroides); kanamycin (25 μg ml-1); and tetracycline (20 μg ml-1) (for E. coli).
Reactive oxygen species (ROS) generation was measured using an oxidation-sensitive fluorescent probe, 2,7-dihydrodichlorofluorescein diacetate (DCFH-DA; Molecular Probes). Cells were incubated with the probe at a final concentration of 10 μM for 30 min. The fluorescence intensities (excitation 492 nm, emission 525 nm) were evaluated in an Infiniti M200 microplate reader (Tecan).
RNA isolation and quality assignment
R. sphaeroides was grown in presence or absence of external iron in triplicate cultures inoculated separately from three independent starter cultures. Cell samples were rapidly cooled on ice and harvested by cooled centrifugation. Total RNA from exponentially growing cultures with an OD660 of 0.5 (oxic conditions) or 0.25 (anoxic conditions) was isolated using the hot phenol method, followed by two chloroform/isoamyl alcohol treatments and precipitation with sodium acetate and ethanol. For quantitative real-time RT-PCR, RNA was isolated using the peqGOLD TriFast™ Kit (Peqlab) as described by the manufacturer. Hereupon, RNA was treated with DNase I (Invitrogen) to remove contaminating DNA. After DNA digestion, RNA was either purified by standard procedures using a mixture of phenol/chloroform/isoamyl alcohol and chloroform/isoamyl alcohol (for RT-PCR) or RNeasy® MinElute™ spin columns (Qiagen) (for microarray and RNAseq). RNA was resolved in RNase free water (Roth), and concentrations were determined using a NanoDrop 1000 Spectrophotometer (Peqlab). The absence of genomic DNA contamination was assessed by PCR using primers targeting gloB (RSP_0799) as described previously . Polyacrylamide gels (10%, v/v) containing 7 M urea were prepared to assess RNA quality.
Quantitative real-time RT-PCR
The One-Step Brilliant III QRT-PCR Master Mix Kit (Agilent) was used for reverse transcription followed by PCR as described in the manufacturer’s manual. RT-PCR samples containing 4 ng of total RNA per μl were run in a Rotor-Gene 3000 real-time PCR cycler (Corbett Research) for relative quantification of mRNAs in each of three independent experiments. The oligodeoxynucleotide sequences used for amplification are listed in Additional file 4: Table S5. Crossing points (Cp) with a fluorescence threshold of 0.002 were visualised with the Rotor-Gene software 6.0 (Corbett Research). Statistical comparisons were performed using the student‘s t test and p-values ≤0.05 were considered statistically significant. The expression of target genes was calculated relative to the control under normal iron conditions and normalised to control gene rpoZ .
Microarray analysis was performed as described before . In brief, 2 μg of total RNA of three independent experiments of control and iron-limiting cultures was pooled, chemically labelled either with Cy3 or Cy5 and hybridised to one array. Transcriptome profiles were analysed on two arrays including six biological replicates. Differentially labelled RNA samples were mixed and competitively hybridised to microarrays. Hybridisations and scanning were performed according to the specifications from Agilent. Multiarray analysis and normalisation according to LOESS were accomplished with the Bioconductor package Limma for R and performed as described elsewhere [42, 43]. On the basis of calculated MA plots, genes were considered reliable if the average signal intensity [A-value: 1/2 log2 (Cy3 × Cy5)] was ≥ 12. Fold changes were calculated using MS Excel (Microsoft). The data shown in this study represent the results from two individual microarrays (biological replicates), each containing a pool of three independent experiments for each sample. The microarray data have been deposited in NCBI’s Gene Expression Omnibus  and are accessible through GEO Series accession number GSE47182 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE47182).
Library construction and sequencing
The libraries were generated by Vertis Biotechnologie AG (Munich, Germany). The treated and untreated RNA samples were poly(A)-tailed by using poly(A) polymerase. The 5′-PPPs were removed using tobacco acid pyrophosphatase (TAP) followed by the ligation of the RNA adapter to the 5′-monophosphate of the RNA. First-strand cDNA synthesis was performed with an oligo(dT)-adapter primer and M-MLV reverse transcriptase. The resulting cDNA was PCR-amplified to reach a concentration of 20–30 ng/μl using a high fidelity DNA polymerase. The cDNA was purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics) and was analysed by capillary electrophoresis.
The primers used for PCR amplification were designed for TruSeq sequencing according to the recommendations of Illumina. The following adapter sequences flank the cDNA inserts: TrueSeq_Sense_primer 5′-AAT GAT ACG GCG ACC GAG ATC TAC ACT CTT TCC CTA CAC GAC GCT CTT CCG ATC T-3′; TrueSeq_Antisense_NNNNNN_primer (NNNNNN = Barcode) 5′-CAA GCA GAA GAC GGC ATA CGA GAT-NNNNNN-GTG ACT GGA GTT CAG ACG TGT GCT CTT CCG ATC (dT25)-3′. The combined length of the flanking sequences is 146 bases. The libraries were sequenced with an Illumina HiSeq machine with 100 cycles in single-end mode. The data shown in this study represent the results from one RNAseq experiment containing a pool of three independent experiments for each sample.
The pooled sequence reads from the RNAseq were demultiplexed, and the adapter sequences were removed. After that, the reads in Fastq format were quality trimmed using fastq_quality_trimmer (from the FastX suite version 0.0.13 - http://hannonlab.cshl.edu/fastx_toolkit/) with a cut-off Phred score of 20 and converted to fasta format using fastq_to_fasta (also from the FastX suite). The read processing (poly(A) removal, size filtering (min 12 nt length), statistics generation, gene-wise read counting, RPKM and coverage calculation as well as normalisation was performed using READemption  with default parameters using segemehl version 0.1.3  for the read alignment (accuracy parameter set to 95%). Differential gene expression analysis was performed with DESeq 1.12.0  only considering genes that show in at least one the conditions a RPKM  value of ≥ 5.0.
The sequences and annotations of the replicons with the following GenBank Ids were used as references: CP000143.1, CP000144.1, CP000145.1, CP000146.1, CP000147.1, DQ232586.1, DQ232587.1 (Additional file 5: Table S6). The demultiplexed Fastq files and coverage files in wiggle format have been deposited in NCBI’s Gene Expression Omnibus  and are accessible through GEO Series accession number GSE47182 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE47182).
Cluster analysis was performed using MeV (Multi Experiment Viewer version 4.7.4) from the TM4 Microarray Software Suite [44–46]. Clustering was based on k-means (KMC method) according to Euclidean distance with a maximum of 50 iterations. Strongly regulated genes (log2 ratio > 1 or < -1) were grouped into six clusters and visualised as a scatter-plot (Figure 1B).
No ethical approval was required in connection with the study as there was no research involving human subjects or vertebrates.
Availability of supporting data
All data sets supporting the results of this article are accessible in the NCBI’s GEO database through GEO Series accession number GSE47182 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE47182).
We thank Cynthia Sharma (University of Würzburg) for her help with RNAseq and data analyses. This work was supported by Deutsche Forschungsgemeinschaft (Kl563/25).
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