Skip to main content

A low-density SNP array for analyzing differential selection in freshwater and marine populations of threespine stickleback (Gasterosteus aculeatus)



The threespine stickleback (Gasterosteus aculeatus) has become an important model species for studying both contemporary and parallel evolution. In particular, differential adaptation to freshwater and marine environments has led to high differentiation between freshwater and marine stickleback populations at the phenotypic trait of lateral plate morphology and the underlying candidate gene Ectodysplacin (EDA). Many studies have focused on this trait and candidate gene, although other genes involved in marine-freshwater adaptation may be equally important. In order to develop a resource for rapid and cost efficient analysis of genetic divergence between freshwater and marine sticklebacks, we generated a low-density SNP (Single Nucleotide Polymorphism) array encompassing markers of chromosome regions under putative directional selection, along with neutral markers for background.


RAD (Restriction site Associated DNA) sequencing of sixty individuals representing two freshwater and one marine population led to the identification of 33,993 SNP markers. Ninety-six of these were chosen for the low-density SNP array, among which 70 represented SNPs under putatively directional selection in freshwater vs. marine environments, whereas 26 SNPs were assumed to be neutral. Annotation of these regions revealed several genes that are candidates for affecting stickleback phenotypic variation, some of which have been observed in previous studies whereas others are new.


We have developed a cost-efficient low-density SNP array that allows for rapid screening of polymorphisms in threespine stickleback. The array provides a valuable tool for analyzing adaptive divergence between freshwater and marine stickleback populations beyond the well-established candidate gene Ectodysplacin (EDA).


It is becoming increasingly evident that evolution is not just a long-term process on the scale of millennia; contemporary evolution can take place over just a few generations [1, 2]. Similarly, the importance of parallel evolution in populations facing similar environmental conditions and the role of gene reuse (or lack thereof) in this process is increasingly discussed [36]. The threespine stickleback (Gasterosteus aculateus) is distributed throughout the northern Hemisphere and shows extensive morphological and ecological variation [7]. Numerous resources, including its genome sequence are available, and the species has emerged as one of the most important models for studying both contemporary [8, 9] and parallel evolution [1016]. Adaptation to freshwater and marine environments, respectively, has received particular attention due to the differences of plate morphology in the two environments and the finding of Ectodysplacin (EDA) as a candidate locus [10, 16, 17]. Nevertheless, other regions of the genome than that harboring EDA also show footprints of differential selection in freshwater and marine habitats [1113], and in some cases these encompass non-coding and presumably regulatory regions [13].

In some geographical regions, notably Northern Europe, the patterns of divergence between marine and freshwater populations of threespine sticklebacks appear less distinct than in other regions, possibly reflecting gene flow overcoming selection [18, 19]. However, this has mainly been studied with specific focus on EDA and/or lateral plate morphology. Screening of adaptive divergence at other chromosomal regions could be achieved by whole genome sequencing or RAD (Restriction Associated DNA) sequencing [11, 13], though this precludes studies requiring large sample sizes. Also, a medium-density SNP chip has previously been constructed, encompassing 3,072 markers [12]. However in some situations, where analysis of many individuals from many localities is required, it would be preferable to invest more in sample size than in genomic resolution. This involves cases where hundreds of individuals are analyzed in order to assess e.g. temporal changes of allele frequencies as a result of selection, or hybrid zone dynamics [1820]. Microsatellite loci have been developed that mark chromosomal regions under differential selection in freshwater and marine environments [16, 17], but development of a SNP array would allow for even faster and cost-efficient genotyping. In the present study, we therefore aimed at generating a low-density SNP array encompassing markers of chromosomal regions under differential freshwater-marine selection along with neutral markers for background, thus providing a resource for extensive studies of parallel evolution and marine-freshwater hybrid zone dynamics.

We identified SNPs based on RAD sequencing of one marine and two isolated freshwater populations. Based on these data we chose 96 SNPs for inclusion in the array. In order to validate the array we also analyzed a sample of threespine sticklebacks from a Danish river that represents a mixture of marine and freshwater morphs.


Ethical statement

Sampling of sticklebacks took place in accordance with Danish law and regulations. Threespine stickleback is not included in the Directive “Bekendtgørelse om fredning af visse dyre- og plantearter mv., indfangning af og handel med vildt og pleje af tilskadekommet vildt” (Directive on Protection of Certain Animal and Plant Species, Catch and Trade of Game, and Nursing of Wounded Game) by the Danish Ministry of the Environment. Catch of sticklebacks is therefore permitted unless it involves so high numbers of individuals that it would significantly affect the ecosystem, which was clearly not the case in this study. The fish were euthanized using an overdose of benzocaine and were subsequently stored in 96% ethanol.

Sampled localities

Sixty threespine sticklebacks, 20 from each site, were sampled by cast nets or minnow traps from three localities in Jutland, Denmark: 1) Lake Hald, a 3.3 km2 freshwater lake, 2) a small unnamed freshwater pond (ca. 0.01 km2) near the town of Hadsten and 3) the Mariager Fjord, a marine environment (see Figure 1). These individuals were analyzed using RAD sequencing [11, 21] in order to identify SNPs. 4) An additional 96 individuals were sampled close to the outlet of the Odder River, Jutland, Denmark (see Figure 1). Individuals from this estuarine population were genotyped in order to validate the generated SNP array. The first two samples (Lake Hald and Hadsten) consisted of morphs with low numbers of lateral plates (“low-plated”), as typically observed in freshwater [10]. The third, marine sample consisted of the typical marine morph with high numbers of lateral plates (“high-plated”), whereas the fourth estuarine population consisted of a mixture of low and high-plated morphs.

Figure 1
figure 1

Map showing the location of sampled three-spine stickleback populations in Jutland, Denmark.

RAD sequencing and SNP identification

Genomic DNA was extracted from muscle tissue using standard phenol-chloroform extraction. RAD sequencing was conducted by Beijing Genomics Institute (BGI, Hong Kong, China). The procedures for construction of libraries and Illumina HiSeq paired-end sequencing followed those described for European eel (A. anguilla) by Pujolar et al.[22], except for the fact that samples were digested with the restriction enzyme SbfI instead of EcoRI. Sequence lengths were 90 bp.

Only the first reads (with the restriction site) were used in subsequent analyses due to low coverage of the second reads (not containing the restriction site). The sequence reads were sorted according to their unique barcode tag and filtered and trimmed using the FASTX Toolkit ( Final read lengths were trimmed to 75 nucleotides to avoid an increase of sequencing errors in the tail ends [22]. Reads of poor quality (with a Phred score < 10 per nucleotide position) were removed. Reads were subsequently aligned to the stickleback genome using Bowtie version 0.12.8 [23] with a maximum of 2 mismatches allowed between individual reads and the genome sequence. Alignments were suppressed for a particular read if more than one reportable alignment was present. This was done in order to minimize the occurrence of paralogous sequences in the data.

The reference-aligned data were subsequently used to identify SNPs and call genotypes. For this purpose we used the pipeline in Stacks[24], implementing a maximum-likelihood model for SNP calling and filtering out RAD loci within individuals with a coverage < 10x. Furthermore, we required loci to be genotyped in at least 70% of the individuals from each population sample. Loci with a sequencing depth > 80x or exhibiting three alleles within individuals were also removed in order to avoid paralogs.

FST for each SNP between pairs of marine and freshwater populations was estimated using Populations implemented in Stacks [[24]]. The same pipeline was used for estimating sliding windows FST across 150,000 bp along each chromosome, based on a Gaussian Kernel smoothing function. Finally, the smoothed FST values were plotted using the R package [25].

SNP low-density array design

Based on the outcome of the analysis of RAD data we selected 96 SNPs for inclusion in the low density SNP array. We selected SNPs 1) exhibiting high genetic differentiation between the two freshwater and marine populations, both at the individual SNPs and based on smoothed FST values, indicating possible diversifying selection; and 2) SNPs outside regions of elevated differentiation, presumably reflecting neutral markers. We used the threespine stickleback genome sequence to extend the flanking sequence to at least 100 bp to allow for optimal primer design. We also searched for possible candidate loci marked by the SNPs using the stickleback genome browser (, in which many genes are already annotated by name and putative orthology. The best Blast hit was used to assess the putative orthologous gene. The putative orthology relationships of the remaining genes, i.e. those that have not yet been annotated, were further analyzed by a Blast comparison of their predicted protein sequence against the NCBI protein database. The function of the candidate genes was assessed using two searchable databases: The AmiGO 2 GO browser and an integrated database of human genes that also provides putative orthology with other vertebrates (

The selected 96 SNPs were genotyped in 96 individuals from the Odder River population on 96.96 Dynamic Arrays (Fluidigm Corporation, SanFrancisco, CA, USA), using the Fluidigm EP1 instrumentation according to the manufacturer’s recommendations. The Fluidigm system uses nano-fluidic circuitry to simultaneously genotype up to 96 individuals at 96 loci (see [26] for a description of the Fluidigm system methodology). Genotypes were called using the Fluidigm SNP Genotyping Analysis software. We used Genalex 6.5 [27] to estimate expected and observed heterozygosity and test for Hardy-Weinberg equilibrium at each locus. Significance levels were adjusted using False Discovery Rate correction [28].


RAD sequencing

RAD sequencing generated from 1.06 to 8.22 million reads per individual, with an average of 2.8 million reads. The mean depth of sequencing was 44.59. The number of reads retained through each step of the analysis is listed in Table 1. After all filtering steps in Stacks and post-filtering to remove possible paralogs, 19,793 loci were retained that represent 33,993 SNPs.

Table 1 RAD sequencing statistics

Genome-wide FST was 0.056 between the Lake Hald (freshwater) and Mariager Fjord (marine) populations and 0.111 between Hadsten (freshwater) and the Mariager Fjord populations. Sliding window analysis of FST revealed high peaks of differentiation, potentially marking chromosome regions under differential selection in marine and freshwater. Twenty-one peaks distributed across 15 different chromosomes were thus identified in the Hadsten – Mariager Fjord comparison, whereas 15 peaks across 9 different chromosomes were revealed in the case of Hald – Mariager Fjord (Figure 2). Though most of these identified regions were found in both marine-freshwater population comparisons, some of them were found in only one of the two pairs.

Figure 2
figure 2

Genome-wide distribution of smoothed F ST estimates for pairwise comparisons between the Hadsten/Mariager and Lake Hald/Mariager populations. Grey boxes indicate boundaries of chromosomes (from 1 to 21) and successive chromosomes are denoted by different shades of grey. Peaks above the red line correspond to the chromosomal regions exhibiting elevated differentiation that are referred to throughout the text, from which candidate SNPs for directional selection were chosen. Most peaks of elevated differentiation were shared between the two population comparisons, but in the cases where elevated differentiation was only observed in a single comparison this is denoted by a red dot.

SNP low-density array design

We selected 96 SNPs for inclusion in the array. Twenty-six were chosen at random, but randomly distributed across 19 chromosomes to represent putatively neutral markers, with FST ranging from 0 to 0.18 between the two independent freshwater populations and the marine sample. The remaining 70 SNPs were chosen to reflect all of the high differentiation regions identified by the sliding-window approach. Some of the SNPs included represented high-differentiation peaks observed in both marine-freshwater population comparisons, but some were found to be outliers in only one of the two comparisons (Figure 2). The SNPs presumably under (hitchhiking) selection exhibited FST values ranging from 0.24 to 0.93 between Hadsten and Mariager Fjord and from 0.27 to 0.78 between Lake Hald and Mariager Fjord (Table 2). The number of outlier SNPs per chromosome ranged from 1 to 7. Considering all SNPs (neutral and under possible selection), each chromosome was represented by at least 4 SNPs.

Table 2 List of the 96 selected SNPs

The potential candidate loci for the SNPs under selection, along with their ontological relationships (when available) are listed in Table 3. This table lists 71 candidate genes identified from 20 chromosomes, 7 of which are involved in functions related to morphogenesis and growth, 2 related to skeletal biology, 5 related to kidney functions and 11 involved in osmoregulation. The remaining 46 candidate genes are associated to other functional categories, such as immune response, hormonal system or vision (see Table 3 for details). We chose not to include SNPs close to EDA, as this gene is usually analyzed using an indel (insertion-deletion) marker (Stn381) that is not suitable for inclusion in the array [10, 18]. Among the SNPs included in the array, the one closest to the EDA gene is situated more than 2.3 Mb away and therefore not showing tight linkage relationships. All sequences along with SNP positions used for generating the array are listed in Additional file 1: Table S1.

Table 3 Identified candidate genes for freshwater vs. marine adaptation in threespine stickleback

Validation of the array based on analysis of 96 individuals from the Odder River provided results for all SNPs. However, there was significant drop-out at the markers 19976 and 26062 indicating technical problems with these two SNPs. Seven loci showed low expected heterozygosity (He < 0.05), whereas mean He across all loci was 0.226 (Additional file 2: Table S2). Twelve loci showed deviations from Hardy-Weinberg equilibrium, possibly reflecting that samples were taken in a mixture zone between freshwater and marine sticklebacks (Additional file 2: Table S2). Genotypic data for all SNPs and individuals are provided in Genalex 6.5 [27] format in Additional file 3.


Development and utility of low density SNP chips

We are currently witnessing a transition from population genetics to population genomics, particularly mediated by the development of Next Generation Sequencing [2931]. Whereas this allows for addressing research questions at the level of entire genomes [13, 32, 33], the methods used also provide resources that can be used for generating markers for more specific purposes. For instance, Hess et al. [34] conducted a population genomics study of Pacific lampreys using RAD sequencing, and subsequently used RAD data to construct a 96 SNP chip including markers that could be used for species identification, for general studies of genetic population structure and for screening loci previously suggested to be under directional selection [35]. Similarly, Pujolar et al.[36] used RAD sequencing of European (Anguilla anguilla) and American eel (A. rostrata) to develop a 96 SNP chip encompassing markers diagnostic for the two species. This resource was subsequently used for tracing hybridization between the two species several generations back in time.

The SNP chip developed in the current study similarly distills information derived from RAD sequencing. The 96 SNPs encompass markers of chromosomal regions that exhibit elevated differentiation in comparisons involving a marine population and two independent freshwater stickleback populations, possibly reflecting diversifying selection. It therefore provides a useful resource for analyzing differential adaptive responses in freshwater and marine sticklebacks and the extent to which this reflects parallel evolution. Nevertheless, it also involves some important caveats. First, although there is evidence for geographically widespread parallel evolution and gene reuse when marine sticklebacks colonize freshwater environments [11, 13, 16], there are clearly also examples of non-parallel adaptive responses [16], either reflecting differences in local freshwater environments or different genetic architecture underlying similar phenotypes. Our inclusion of SNPs therefore undoubtedly represents some degree of ascertainment bias [37, 38], particularly in terms of not identifying chromosomal regions under selection in other freshwater populations than those used for identifying SNPs. Second, three-spine stickleback is widespread across the Northern Hemisphere, and there is presumably a geographical limit defined by phylogeographical relationships beyond which many of the SNPs are no longer polymorphic; this can be regarded as another aspect of ascertainment bias. The developed SNP chip may therefore be of primary use in North-Western Europe, encompassing the North Sea and Baltic Sea regions.

Other marker resources have been developed for three-spine stickleback, including a 3,072 SNP chip [12], a resource of 158 microsatellite markers linked to physiologically important genes [17] and a resource of 110 SNPs representing both genic and non-genic regions [39]. Compared to the 3,072 SNP chip [12], the array developed in the present study obviously provides less dense genome coverage, but is also cheaper in running costs and specifically targeted towards freshwater-saltwater adaptation. Compared to the microsatellite resource [17], our 96 SNP array provides faster genotyping. On the other side, marker-by-marker multiallelic microsatellites provide more statistical power than diallelic SNPs [3941]. A further important difference between 1) the microsatellite resource [17] and the 110 SNP resource [39] on the one side and 2) the current 96 SNP array on the other side consists in the choice of markers. Microsatellites and approximately half of the 110 SNPs were chosen based on the criterion that they should be linked to physiologically important genes [17]. In contrast, 70 of the SNPs included in the 96 SNP array were chosen from genomic regions exhibiting elevated differentiation, regardless of their linkage to candidate genes. There is increasing evidence that non-coding DNA may be of functional importance and potentially under selection [13, 4244]. Indeed, 7 of the 70 SNPs under putative directional selection could not be linked to a candidate gene and could therefore potentially mark regulatory regions under selection. In total, our resource can be considered unbiased with respect to prior choice of candidate genes, but can be subject to ascertainment bias given that markers were chosen based on genetic differentiation between a subset of freshwater and marine populations. On the other side, the microsatellite resource by Shimada et al.[17] and a major part of the SNP resource by DeFaveri et al. [39] are specifically targeted towards genes of physiological importance but do not involve ascertainment bias in terms of choosing loci exhibiting high differentiation. Hence, there are pros and cons with both approaches and the choice of markers and methods may depend on the specific study and research question.

Candidate genes for marine and freshwater adaptation

Similar to previous studies undertaking genome-wide scans of threespine sticklebacks [1113, 16, 17], we identified several chromosomal regions that are likely under differential selection in freshwater and marine environments (Figure 2). Comparison of our results with results from whole genome sequencing [12] and RAD sequencing [11] suggests that several of the regions may be the same, thereby also implying that the same candidate genes may be involved. Specifically, there appears to be concordance among the previous and the current study in identifying regions on chromosomes I, IV, VII, IX, XI, XIV, XVI and XX as being involved in freshwater-saltwater adaptation (compare e.g. Figure 2 of the present study with Figure two (a) in [13]).

The identified outlier chromosomal regions harbor a number of candidate genes with functional relationships that are already known to be important for adaptation between freshwater and marine habitats, such as genes affecting bone development, kidney function and osmoregulation (Skeletal Biology: SB; Kidney Function: KF; Osmoregulation: OM ,respectively; see Table 3). We find it interesting that our study reveals two candidate loci (both on chromosome XI; ATP2A2 and ABCA3, see Table 3) putatively implied in ATPase activity, generally associated with salinity tolerance. Other candidate genes related to this ATPase activity have previously been found on chromosome I and in two other regions of chromosome XI [12], and the candidate genes suggested by the current study further emphasize the importance of this physiological trait.

The insulin-like growth factor binding protein 2, IGFBP2 in chromosome I (see Table 3) is another interesting candidate gene observed in the present study that was also suggested as a candidate for freshwater-marine adaptation by Hohenlohe et al.[11]. We also note four highly differentiated SNPs in four different chromosomal regions (Table 3); ADAMTS18 in chromosome II, retinol binding protein 4 (RBP4) in chromosome V, lens fiber membrane intrinsic protein 2 (LIM2) in chromosome VII and the retinoic X receptor alpha (RXRA) in chromosome XVI (FST values ranging from 0.54 to 0.8, Table 2) that could be involved in vision. This could reflect adaptation to different light environments, in the present case between freshwater and marine habitats, as previously observed in other marine organisms [45, 46].

As our SNP resource was specifically designed based on RAD sequencing data, there are a number of candidate genes and chromosomal regions that will inevitably not be represented. First, some candidate genes and SNPs may only be regionally important, as discussed previously. Second, RAD sequencing using the 8-base cutter SbfI obviously provides less resolution than e.g. whole genome sequencing, and there may be regions and candidate genes showing elevated differentiation that have not been detected. Our SNP resource can be regarded as a reduced representation of outlier regions detected by RAD sequencing, which by itself represents a reduced representation of the whole genome. Obviously, the SNP resource can be supplemented by other previously identified candidate genes and markers, and conversely it represents a supplement to the markers and resources already available [10, 12, 13, 17].


We have constructed a low density SNP array that encompasses both neutral SNPs for background and SNPs representing genomic regions that exhibit differentiation compatible with diversifying selection in freshwater and marine environments. We find this resource to be particularly useful for addressing research questions that require high sample sizes, e.g. several hundreds, which would in most cases not be feasible for whole genome sequencing and RAD sequencing. For instance, this concerns situations where hybrid zone dynamics between freshwater and marine sticklebacks are analyzed along environmental gradients [20]. This may necessitate large sample sizes, e.g. if continuous sampling is conducted in order to identify clinal shifts of allele frequencies [47] or define populations based on neutral or adaptive markers [48]. Also, studies of selection based on detecting allele frequency change using analysis of temporal samples, e.g. taken at different time points within a year [18], may require analysis of many samples and large sample sizes. We find our SNP array to be particularly useful in such situations, as it allows for studies going beyond analyzing EDA and instead targeting multiple genomic regions involved in differential adaptation to freshwater and marine environments. We specifically intend to use the SNP array for testing the hypothesis that gene flow from marine populations overrides selection in freshwater sticklebacks in coastal regions [18]. If this is indeed the case, then this should not only be detectable at the EDA locus but also at other genes involved in adaptive responses, including those represented in our array.

Availability of supporting data

Sequence reads have been deposited in NCBI’s Sequence Read Archive (Accession number: SAMN0255793).



ATP-binding cassette, sub-family A (ABC1), member 3


ADAM metallopeptidase with thrombospondin type 1 motif, 18


ATPase, Ca++ transporting, cardiac muscle, slow twitch 2




Insulin-like growth factor binding protein 2




Kidney function


Lens fiber membrane intrinsic protein 2




Restriction site associated DNA


Retinol binding protein 4


Retinoic X receptor alpha


Skeletal biology


Single nucleotide polymorphism.


  1. Stockwell CA, Hendry AP, Kinnison MT: Contemporary evolution meets conservation biology. Trends Ecol Evol. 2003, 18: 94-101. 10.1016/S0169-5347(02)00044-7.

    Article  Google Scholar 

  2. Kinnison MT, Hendry AP, Stockwell CA: Contemporary evolution meets conservation biology II: Impediments to integration and application. Ecol Res. 2007, 22: 947-954. 10.1007/s11284-007-0416-6.

    Article  Google Scholar 

  3. Conte GL, Arnegard ME, Peichel CL, Schluter D: The probability of genetic parallelism and convergence in natural populations. P R Soc B. 2012, 279: 5039-5047. 10.1098/rspb.2012.2146.

    Article  Google Scholar 

  4. Miller MR, Brunelli JP, Wheeler PA, Liu SX, Rexroad CE, Palti Y, Doe CQ, Thorgaard GH: A conserved haplotype controls parallel adaptation in geographically distant salmonid populations. Mol Ecol. 2012, 21: 237-249. 10.1111/j.1365-294X.2011.05305.x.

    Article  PubMed Central  PubMed  Google Scholar 

  5. Gagnaire PA, Pavey SA, Normandeau E, Bernatchez L: The genetic architecture of reproductive isolation during speciation-with-gene-flow in lake whitefish species pairs asessed by RAD sequencing. Evolution. 2013, 67: 2483-2497. 10.1111/evo.12075.

    Article  PubMed  Google Scholar 

  6. Hoekstra HE, Hirschmann RJ, Bundey RA, Insel PA, Crossland JP: A single amino acid mutation contributes to adaptive beach mouse color pattern. Science. 2006, 313: 101-104. 10.1126/science.1126121.

    Article  CAS  PubMed  Google Scholar 

  7. McKinnon JS, Rundle HD: Speciation in nature: the threespine stickleback model systems. Trends Ecol Evol. 2002, 17: 480-488. 10.1016/S0169-5347(02)02579-X.

    Article  Google Scholar 

  8. Bell MA, Aguirre WE, Buck NJ: Twelve years of contemporary armor evolution in a threespine stickleback population. Evolution. 2004, 58: 814-824. 10.1111/j.0014-3820.2004.tb00414.x.

    Article  PubMed  Google Scholar 

  9. Le Rouzic A, Østbye K, Klepaker TO, Hansen TF, Bernatchez L, Schluter D, Vollestad LA: Strong and consistent natural selection associated with armour reduction in sticklebacks. Mol Ecol. 2011, 20: 2483-2493. 10.1111/j.1365-294X.2011.05071.x.

    Article  PubMed  Google Scholar 

  10. Colosimo PF, Hosemann KE, Balabhadra S, Villarreal G, Dickson M, Grimwood J, Schmutz J, Myers RM, Schluter D, Kingsley DM: Widespread parallel evolution in sticklebacks by repeated fixation of ectodysplasin alleles. Science. 2005, 307: 1928-1933. 10.1126/science.1107239.

    Article  CAS  PubMed  Google Scholar 

  11. Hohenlohe PA, Bassham S, Etter PD, Stiffler N, Johnson EA, Cresko WA: Population genomics of parallel adaptation in threespine stickleback using sequenced RAD tags. PLoS Genet. 2010, 6: 23-

    Article  Google Scholar 

  12. Jones FC, Chan YF, Schmutz J, Grimwood J, Brady SD, Southwick AM, Absher DM, Myers RM, Reimchen TE, Deagle BE, Schluter D, Kingsley DM: A genome-wide SNP genotyping array reveals patterns of global and repeated species-pair divergence in sticklebacks. Curr Biol. 2012, 22: 83-90. 10.1016/j.cub.2011.11.045.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  13. Jones FC, Grabherr MG, Chan YF, Russell P, Mauceli E, Johnson J, Swofford R, Pirun M, Zody MC, White S, Birney E, Searle S, Schmutz J, Grimwood J, Dickson MC, Myers RM, Miller CT, Summers BR, Knecht AK, Brady SD, Zhang HL, Pollen AA, Howes T, Amemiya C, Lander ES, Di Palma S, Lindblad-Toh K, Kingsley DM: The genomic basis of adaptive evolution in threespine sticklebacks. Nature. 2012, 484: 55-61. 10.1038/nature10944.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  14. Mäkinen HS, Cano M, Merila J: Identifying footprints of directional and balancing selection in marine and freshwater three-spined stickleback (Gasterosteus aculeatus) populations. Mol Ecol. 2008, 17 (15): 3565-3582. 10.1111/j.1365-294X.2008.03714.x.

    Article  PubMed  Google Scholar 

  15. Rogers SM: Mapping the genomic architecture of ecological speciation in the wild: does linkage disequilibrium hold the key?. Mol Ecol. 2012, 21: 5155-5158. 10.1111/mec.12019.

    Article  PubMed  Google Scholar 

  16. DeFaveri J, Shikano T, Shimada Y, Goto A, Merila J: Global analysis of genes involved in freshwater adaptation in threespine sticklebacks (Gasterosteus aculeatus). Evolution. 2011, 65: 1800-1807. 10.1111/j.1558-5646.2011.01247.x.

    Article  PubMed  Google Scholar 

  17. Shimada Y, Shikano T, Merila J: A high incidence of selection on physiologically important genes in the three-spined stickleback, Gasterosteus aculeatus. Mol Biol Evol. 2011, 28: 181-193. 10.1093/molbev/msq181.

    Article  CAS  PubMed  Google Scholar 

  18. Raeymaekers JA, Konijnendijk N, Larmuseau MH, Hellemans B, De Meester L, Volckaert FA: A gene with major phenotypic effects as a target for selection vs. homogenizing gene flow. Mol Ecol. 2014, 23: 162-181. 10.1111/mec.12582.

    Article  CAS  PubMed  Google Scholar 

  19. McCairns RJS, Bernatchez L: Landscape genetic analyses reveal cryptic population structure and putative selection gradients in a large-scale estuarine environment. Mol Ecol. 2008, 17: 3901-3916. 10.1111/j.1365-294X.2008.03884.x.

    Article  PubMed  Google Scholar 

  20. Jones FC, Brown C, Pemberton JM, Braithwaite VA: Reproductive isolation in a threespine stickleback hybrid zone. J Evol Biol. 2006, 19: 1531-1544. 10.1111/j.1420-9101.2006.01122.x.

    Article  CAS  PubMed  Google Scholar 

  21. Baird NA, Etter PD, Atwood TS, Currey MC, Shiver AL, Lewis ZA, Selker EU, Cresko WA, Johnson EA: Rapid SNP discovery and genetic mapping using sequenced RAD markers. PLoS One. 2008, 3: e3376-10.1371/journal.pone.0003376.

    Article  PubMed Central  PubMed  Google Scholar 

  22. Pujolar JM, Jacobsen MW, Frydenberg J, Als TD, Larsen PF, Maes GE, Zane L, Jian JB, Cheng L, Hansen MM: A resource of genome-wide single-nucleotide polymorphisms generated by RAD tag sequencing in the critically endangered European eel. Mol Ecol Resour. 2013, 13: 706-716. 10.1111/1755-0998.12117.

    Article  CAS  PubMed  Google Scholar 

  23. Langmead B, Trapnell C, Pop M, Salzberg SL: Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 2009, 10: R25-10.1186/gb-2009-10-3-r25.

    Article  PubMed Central  PubMed  Google Scholar 

  24. Catchen J, Hohenlohe PA, Bassham S, Amores A, Cresko WA: Stacks: an analysis tool set for population genomics. Mol Ecol. 2013, 22: 3124-3140. 10.1111/mec.12354.

    Article  PubMed Central  PubMed  Google Scholar 

  25. Hoekstra HE: Genomics: Stickleback is the catch of the day. Nature. 2012, 484: 46-47. 10.1038/484046a.

    Article  CAS  PubMed  Google Scholar 

  26. Seeb JE, Pascal CE, Ramakrishnan R, Seeb LW: SNP genotyping by the 5′-nuclease reaction: advances in high-throughput genotyping with nonmodel organisms. Methods Mol Biol. 2009, 578: 277-292. 10.1007/978-1-60327-411-1_18.

    Article  CAS  PubMed  Google Scholar 

  27. Peakall R, Smouse PE: GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics. 2012, 28: 2537-2539. 10.1093/bioinformatics/bts460.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  28. Benjamini Y, Yekutieli D: The control of the false discovery rate in multiple testing under dependency. Ann Stat. 2001, 29: 1165-1188. 10.1214/aos/1013699998.

    Article  Google Scholar 

  29. Ellegren H: Genome sequencing and population genomics in non-model organisms. Trends Ecol Evol. 2014, 29: 51-63. 10.1016/j.tree.2013.09.008.

    Article  PubMed  Google Scholar 

  30. Allendorf FW, Hohenlohe PA, Luikart G: Genomics and the future of conservation genetics. Nat Rev Genet. 2010, 11: 697-709. 10.1038/nrg2844.

    Article  CAS  PubMed  Google Scholar 

  31. Davey JW, Hohenlohe PA, Etter PD, Boone JQ, Catchen JM, Blaxter LM: Genome-wide genetic marker discovery and genotyping using next-generation sequencing. Nat Rev Genet. 2011, 12: 499-510. 10.1038/nrg3012.

    Article  CAS  PubMed  Google Scholar 

  32. Renaut S, Grassa CJ, Yeaman S, Moyers BT, Lai Z, Kane NC, Bowers JE, Burke JM, Rieseberg LH: Genomic islands of divergence are not affected by geography of speciation in sunflowers. Nat Commun. 2013, 4: 1827-

    Article  CAS  PubMed  Google Scholar 

  33. Moura AE, Janse van Rensburg C, Pilot M, Tehrani A, Best PB, Thornton M, Plon S, de Bruyn PJ, Worley KC, Gibbs RA, Dahlheim ME, Hoelzel AR: Killer whale nuclear genome and mtDNA reveal widespread population bottleneck during the last glacial maximum. Mol Biol Evol. 2014, 31: 1121-1131. 10.1093/molbev/msu058.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  34. Hess JE, Campbell NR, Close DA, Docker MF, Narum SR: Population genomics of Pacific lamprey: adaptive variation in a highly dispersive species. Mol Ecol. 2013, 22: 2898-2916. 10.1111/mec.12150.

    Article  CAS  PubMed  Google Scholar 

  35. Hess JE, Campbell NR, Docker MF, Baker C, Jackson A, Lampman R, McIlraith B, Moser ML, Statler DP, Young WP, Wildbill AJ, Narum SR: Use of genotyping by sequencing data to develop a high-throughput and multifunctional SNP panel for conservation applications in Pacific lamprey. Mol Ecol Resour. 2014, In press

    Google Scholar 

  36. Pujolar JM, Jacobsen MW, Als TD, Frydenberg J, Magnussen E, Jonsson B, Jiang X, Cheng L, Bekkevold D, Maes GE, Bernatchez L, Hansen MM: Assessing patterns of hybridization between North Atlantic eels using diagnostic single-nucleotide polymorphisms. Heredity. 2014, 112: 627-637. 10.1038/hdy.2013.145.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  37. Albrechtsen A, Nielsen FC, Nielsen R: Ascertainment biases in SNP chips affect measures of population divergence. Mol Biol Evol. 2010, 27: 2534-2547. 10.1093/molbev/msq148.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  38. Rosenblum EB, Novembre J: Ascertainment bias in spatially structured populations: A case study in the eastern fence lizard. J Hered. 2007, 98: 331-336. 10.1093/jhered/esm031.

    Article  PubMed  Google Scholar 

  39. DeFaveri J, Viitaniemi H, Leder E, Merila J: Characterizing genic and nongenic molecular markers: comparison of microsatellites and SNPs. Mol Ecol Res. 2013, 13: 377-392. 10.1111/1755-0998.12071.

    Article  CAS  Google Scholar 

  40. Morin PA, Luikart G, Wayne RK: SNPs in ecology, evolution and conservation. Trends Ecol Evol. 2004, 19: 208-216. 10.1016/j.tree.2004.01.009.

    Article  Google Scholar 

  41. Glover KA, Hansen MM, Lien S, Als TD, Hoyheim B, Skaala O: A comparison of SNP and STR loci for delineating population structure and performing individual genetic assignment. BMC Genet. 2010, 11: 12-

    Article  Google Scholar 

  42. Chorev M, Carmel L: The function of introns. Front Genet. 2012, 3: 55-

    Article  PubMed Central  PubMed  Google Scholar 

  43. Hebert FO, Renaut S, Bernatchez L: Targeted sequence capture and resequencing implies a predominant role of regulatory regions in the divergence of a sympatric lake whitefish species pair (Coregonus clupeaformis). Mol Ecol. 2013, 22: 4896-4914. 10.1111/mec.12447.

    Article  CAS  PubMed  Google Scholar 

  44. The ENCODE Project Consortium: An integrated encyclopedia of DNA elements in the human genome. Nature. 2012, 489: 57-74. 10.1038/nature11247.

    Article  PubMed Central  Google Scholar 

  45. Audzijonyte A, Pahlberg J, Viljanen M, Donner K, Vainola R: Opsin gene sequence variation across phylogenetic and population histories in Mysis (Crustacea: Mysida) does not match current light environments or visual-pigment absorbance spectra. Mol Ecol. 2012, 21: 2176-2196. 10.1111/j.1365-294X.2012.05516.x.

    Article  CAS  PubMed  Google Scholar 

  46. Larmuseau MHD, Raeymaekers JAM, Ruddick KG, Van Houdt JKJ, Volckaert FAM: To see in different seas: spatial variation in the rhodopsin gene of the sand goby (Pomatoschistus minutus). Mol Ecol. 2009, 18: 4227-4239. 10.1111/j.1365-294X.2009.04331.x.

    Article  CAS  PubMed  Google Scholar 

  47. Derryberry EP, Derryberry GE, Maley JM, Brumfield RT: hzar: hybrid zone analysis using an R software package. Mol Ecol Resour. 2014, 14: 652-663. 10.1111/1755-0998.12209.

    Article  PubMed  Google Scholar 

  48. Guillot G, Mortier F, Estoup A: GENELAND: a computer package for landscape genetics. Mol Ecol Notes. 2005, 5: 712-715. 10.1111/j.1471-8286.2005.01031.x.

    Article  CAS  Google Scholar 

Download references


We thank Annie Brandstrup, Karen-Lise D. Mensberg and Kristian Meier for technical assistance, Michael Glad for maintenance of computers and the Villum Foundation for funding (grant no. VKR022523 to MMH).

Author information

Authors and Affiliations


Corresponding author

Correspondence to Michael M Hansen.

Additional information

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

ALF and MMH conceived the study. ALF and MMH analyzed the data and wrote the first draft of the manuscript, with subsequent contributions from DB and SHP. SHP acquired samples for the validation of the array and conducted morphological measurements of Odder River samples. YN and JJ conducted RAD sequencing and initial bioinformatics analyses. DB planned and oversaw design and analyses using the Fluidigm array. All authors read and approved the final manuscript.

Electronic supplementary material


Additional file 1: Table S1: Nucleotide sequences for each SNP position. Fifty nucleotides before and after the targeted position are reported in this table. The two nucleotides corresponding to SNP alleles are presented in brackets. (XLSX 15 KB)


Additional file 2: Table S2: Diversity indices estimated for each locus over 96 individuals from Odder river system. Sample Size (N), observed heterozygosity (Ho), expected heterozygosity (He) and outcomes of tests for Hardy-Weinberg equilibrium (HWE test). *significant at 5% level, **significant at 0.01 level, ***significant at 0.001 level. (XLSX 12 KB)


Additional file 3: SNP genotypes for 96 SNPs in 96 sticklebacks. SNP genotype data for 96 SNPs in 96 sticklebacks from the Odder River, Denmark. The data are provided in Genalex 6.5 [27] format. (XLSX 99 kb) (XLSX 100 KB)

Authors’ original submitted files for images

Below are the links to the authors’ original submitted files for images.

Authors’ original file for figure 1

Authors’ original file for figure 2

Rights and permissions

Open Access  This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.

The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.

To view a copy of this licence, visit

The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ferchaud, AL., Pedersen, S.H., Bekkevold, D. et al. A low-density SNP array for analyzing differential selection in freshwater and marine populations of threespine stickleback (Gasterosteus aculeatus). BMC Genomics 15, 867 (2014).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: