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BMC Genomics

Open Access

Cell adhesion molecules have prognostic potential in colorectal carcinoma

  • Asma’a AL-Attas1Email author,
  • Abdulrhaman AlMalki2,
  • Mamdooh Gari1,
  • Shilu Mathew1,
  • Jaudah Al-Maghrabi1,
  • Mahmoud Al-Ahwal3,
  • Mohammed Al-Qahtani1,
  • Ashraf Dallol1 and
  • Abdelbaset Buhmeida1
BMC Genomics201415(Suppl 2):P13

https://doi.org/10.1186/1471-2164-15-S2-P13

Published: 2 April 2014

Background

The cadherin–catenin complex is vital cell adhesion molecules and it plays a fundamental role in maintaining the cells differentiation and the normal architecture of epithelial tissues [1]. Consequently, the disruption of this complex has been shown in carcinomas and correlated with various pathologic and clinical features. This study seeks for molecular markers in colorectal cancer (CRC) that defines those patients at high risk of recurrence and poor outcome. This study is aimed to assess the expression of selected group of cadherin-catenin; E-cadherin, N-cadherin, α-catenin and β-catenin in subset of primary CRC and determine their relation to different clinico-pathological factors and survival [2].

Materials and methods

To achieve this, paraffin blocks of 103 CRC patients were retrieved. The expression of E-cadherin, N-cadherin, α-catenin and β-catenin were analyzed by immunohistochemmistry (IHC) and promoter hypermethylation of E-cadherin gene CDH1 was analyzed using Methy-Light Assay. Statistical analyses were performed to determine the association of these markers with clinico-pathological variables.

Results

The results showed that the expressions of both α-catenin and N-cadherin were significant sign of poor outcome and recurrence as evaluated by univariate Kaplen-Meier for disease-free survival (DFS) (P=0.034, P=0.053). In multivariate survival (Cox) analysis, α-catenin was significantly independent predictor of DFS (P=0.008). On the other hand, β- catenin and E-cadherin exhibits no prognostic power for recurrence and poor survival. Promoter methylation of CDH1 was observed in 45% of our samples and correlation with E-cadherin expression revealed a 60% concordance.

Conclusions

These results implicate the usefulness of α-catenin and N-cadherin in predicting outcome of patients with CRC.

This work was financially supported by King Abdulaziz City for Science and Technology (KACST) under research no (ARP -30-262).

Authors’ Affiliations

(1)
Center of Excellence in Genomic Medicine Research, King Abdulaziz University
(2)
Faculty of Science, Department of Biochemistry, King Abdulaziz University
(3)
Department of Oncology and Scientific Chair of CRC, King AbdulAziz University

References

  1. Hugh T, Dillon S, et al: Cadherin–catenin expression in primary colorectal cancer: a survival analysis. British journal of cancer. 1999, 80 (7): 1046-10.1038/sj.bjc.6690461.PubMed CentralView ArticlePubMedGoogle Scholar
  2. Zhuo H, Jiang K, et al: Overexpression of N-cadherin is correlated with metastasis and worse survival in colorectal cancer patients. Chinese Science Bulletin. 2013, 1-6.Google Scholar

Copyright

© AL-Attas et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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