Volume 15 Supplement 2

Abstracts from the 2nd International Genomic Medicine Conference (IGMC 2013)

Open Access

Drug formulation studies on regulation of BCL-2 family for treatment of autism

BMC Genomics201415(Suppl 2):P38

https://doi.org/10.1186/1471-2164-15-S2-P38

Published: 2 April 2014

Background

BCL2 and Bax are two members of BCL2 family which is responsible for the regulation of apotosis [1, 2]. BCL2 protein duels with its counteracting twin, a partner known as Bax (Figure 1). When Bax is in excess, cells execute a death command. When BCL2 dominates, the program is inhibited and cells survive. The intent is to propose a drug complex which aids in regulation of the BCL2 and Bax levels, aiding in treatment of autism. Navitoclax is known drug responsible for inhibiting BCL2 production for the treatment of cancer. We suggest a drug formulation of navitoclax with a Bax channel locker.
Figure 1

(Left): Regulation of apoptosis pathway by family of BCL-2 proteins

Materials and methods

The structures of Navitoclax (Pubchem ID : 923564-51-6) and Bax channel locker (Pubchem ID :335165-68-9) were taken from Pubchem database. Their binding affinity and energies with target components were analysed using Autodock 4.0.The active site residues in the target proteins BCL2 (Figure 2) and BAX were determined by literature studies and using CastP and Sp align software. The admet properties and pharmacological properties of the drug were calculated using Discovery studio software.
Figure 2

(Top right): BCL-2 protein docked with Navitoclax [PDB Id: 4LXE]

Results

Based on the binding affinities of Navitoclax and Bax Channel Locker with its respective target proteins BCL2 and Bax respectively, the target functional groups to connect the two ligands were shortlisted. It was taken care the responsible target binding sites aren’t affected with the linkage. Four combinations (Figure 3) were created. Their pharmacological properties were predicted insilico to shortlist the best combination. The predicted properties inferred the drug complex to be non-carcinogenic, and non-mutagen and may display moderate irritancy. The novel drug complex can be further modified and analyzed to get drug with lower molecular weight and displaying low to no irritancy.
Figure 3

(Bottom right): Chemical structure of the novel designed drug complex

Conclusions

The admet and chemical properties suggest there are possibilities to consider the new drug complex designed for further analysis.

Authors’ Affiliations

(1)
Faculty of Computing and Information Technology, King Abdulaziz University
(2)
School of Biosciences and Technology, Vellore Institute of Technology
(3)
Faculty of Computing and Information Technology, King Abdulaziz University

References

  1. Belka C, Budach W: Anti-apoptotic Bcl-2 proteins: structure, function and relevance for radiation biology. Int J Radiat Biol. 2002, 78 (8): 643-58.View ArticlePubMedGoogle Scholar
  2. Helen V, Ratajczak : Theoretical aspects of autism: Causes. A review. Journal of Immunotoxicology. 2011, 8 (1): 68-79.View ArticleGoogle Scholar

Copyright

© Tayubi et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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