Volume 15 Supplement 2

Abstracts of the 2nd International Genomic Medical Conference (IGMC 2013)

Open Access

Effect of advancing paternal age on semen parameters and seminal oxidative stress markers in infertile men

  • Saad Alshahrani1, 2,
  • Ashok Agarwal1, 3Email author,
  • Mourad Assidi3, 4,
  • Adel M. Abuzenadah3, 4,
  • Damayanthi Durairajanayagam1, 5,
  • Ahmet Ayaz1,
  • Rakesh Sharma1 and
  • Edmund Sabanegh1
BMC Genomics201415(Suppl 2):P42

https://doi.org/10.1186/1471-2164-15-S2-P42

Published: 2 April 2014

Background

High rate of subfertility and adverse pregnancy related outcomes associated with childbearing are seen after age 40. In contrast to oogenesis, spermatogenesis continues in elderly men. Adult male germ cells pass through significantly more mitotic replications than the germ cells in adult female. In men, there is an age associated increase in the incidence of breaks in sperm DNA, decrease in apoptosis, and a higher frequency of point mutations. Advanced paternal age is associated with an increased time to pregnancy and decreased pregnancy rates. After adjusting for female age, conception during a 12-month period was > 30 percent less likely for men over 40 years of age as compared to men < 30 years of age [1]. Similarly, a five-fold increase in time to pregnancy was reported in men >45 years compared to men <25 years of age [2]. Age of the husband was the most significant factor contributing to a decreased probability of a pregnancy [3]. Advanced paternal age may result in congenital anomalies in progeny due to an increase in new autosomal dominant mutations such as achondroplasia; Apert, Waardenburg, Crouzon, Pfeiffer, and Marfan syndromes [4]. The goal of our study was to investigate the impact of male ageing on semen quality and seminal oxidative stress (OS) markers.

Materials and methods

In this study, we examined the medical records of 472 infertile men referred to our laboratory between 2008 and 2012. Based on their age, patients were divided into group 1: ≤ 30 years (n = 69); group 2: 31-40 years (n = 298); and group 3:> 40 years (n = 105). We evaluated the conventional semen parameters (WHO, 2010) [5] and OS markers: seminal ROS (chemiluminescence assay), total antioxidant capacity (TAC) and sperm DNA damage (TUNEL assay) in all patients.

Results

The mean age of the study subjects was 36.8 ± 6.7 years. No age-related differences were seen in conventional semen parameters (volume, concentration, motility, and morphology) (Table 1). ROS and antioxidant levels were comparable in the 3 groups. Significantly higher levels of sperm DNA damage (19.94 ± 15.30%) was seen in infertile men >40 years compared to men in younger age groups (P = 0.028 and P = 0.027, respectively).

Conclusions

Sperm DNA damage increases with advancing paternal age. Evaluation of sperm DNA damage will help diagnose the underlying cause of poor fertility in some men and assist the clinician in offering correct treatment modality.

Authors’ Affiliations

(1)
Center for Reproductive Medicine, Cleveland Clinic
(2)
College of Medicine, Salman Bin Abdulaziz University
(3)
Center of Excellence in Genomic Medicine Research, King Abdulaziz University
(4)
KACST Technology Innovation Center for Personalized Medicine at, King Abdulaziz University
(5)
MARA University of Technology

References

  1. Ford WC, North K, Taylor H, Farrow A, Hull MG, Golding J: Increasing paternal age is associated with delayed conception in a large population of fertile couples: evidence for declining fecundity in older men. The ALSPAC Study Team (Avon Longitudinal Study of Pregnancy and Childhood). Hum Reprod. 2000, 15: 1703-View ArticlePubMedGoogle Scholar
  2. Hassan MA, Killick SR: Effect of male age on fertility: evidence for the decline in male fertility with increasing age. Fertil Steril. 2003, 79: 1520-View ArticlePubMedGoogle Scholar
  3. Mathieu C, Ecochard R, Bied V, Lornage J, Czyba JC: Cumulative conception rate following intrauterine artificial insemination with husband's spermatozoa: influence of husband's age. Hum Reprod. 1995, 10: 1090-PubMedGoogle Scholar
  4. Auroux M: Age of the father and development. Contracept Fertil Sex. 1993, 21: 382-PubMedGoogle Scholar
  5. World Health Organization: WHO laboratory manual for the examination and processing of human semen. 2010, Geneva, Switzerland, FifthGoogle Scholar

Copyright

© Alshahrani et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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