Volume 15 Supplement 2
Analysis of correlations between zona pellucida birefringence and molecular markers of oocyte developmental competence
© Assidi et al; licensee BioMed Central Ltd. 2014
Published: 2 April 2014
Human infertility is the incapacity of a couple to conceive after one year of unprotected sexual intercourse. Selection of the best gametes for subsequent steps of fertilization and embryo transfer was shown to be the crucial step in infertility treatment procedure [1, 2]. Oocyte selection using morphological criteria has been the gold standard method in assisted reproductive technologies (ART) clinics. Zona Pellucida (ZP) , a filamentous matrix of glycosylated glycoproteins surrounding the oocyte, is one of these morphological criteria of oocyte selection. In fact, ZP thickness and birefringence was reported to be positively correlated with higher ability of the oocyte to achieve successful pregnancy, but this selection approach has limitations in terms of accuracy, objectivity and constancy. Recent studies using OMICs approaches have identified key molecular markers in somatic cells (cumulus and/or granulosa cells) and follicular fluid that quantitatively and non-invasively predict the oocyte quality for better selection, higher pregnancy rates and efficient infertility treatment. These biomarkers could be a valuable reinforcement of the morphological selection criteria widely used in IVF clinics. In this context, this study was designed to study the relationship between some molecular predictors of oocyte quality found by our group and the conventional morphological parameters of oocyte quality. We expect to find a positive correlation between the ZP birefringence and molecular markers of oocyte competence. Such integrative strategy should lead to a powerful combined approach that will precisely predict the oocyte developmental potential, allowing therefore efficient infertility treatment and elective single embryo transfer (eSET).
Materials and methods
Weak correlations between the 7 gene biomarkers of oocyte developmental potential and the ZP birefringence score. It looks that the ZP morphology is associated to a transcriptomic gene pattern that is not directly related to the developmental competence pathway. Further studies using larger lists of candidate markers are required to identify suitable genes that are highly correlated with the morphological criteria, and therefore able to reinforce the accuracy of oocyte selection. Together, the combination of these two approaches should offer a reliable prognostic tool of the best oocyte allowing efficient infertility treatment and successful pregnancy.
- Balaban B, Urman B: Effect of oocyte morphology on embryo development and implantation. Reproductive biomedicine online. 2006, 12 (5): 608-615.View ArticlePubMedGoogle Scholar
- Krisher RL: The effect of oocyte quality on development. J Anim Sci. 2004, 82 (E-Suppl): E14-23.PubMedGoogle Scholar
- Montag M, Schimming T, Koster M, Zhou C, Dorn C, Rosing B, van der Ven H, Ven der Ven K: Oocyte zona birefringence intensity is associated with embryonic implantation potential in ICSI cycles. Reproductive biomedicine online. 2008, 16 (2): 239-244.View ArticlePubMedGoogle Scholar
- MTG: Octax PolarAIDE: Automatic assessment of oocyte quality and beyond. 2010Google Scholar
- Hamel M, Dufort I, Robert C, Gravel C, Leveille MC, Leader A, Sirard MA: Identification of differentially expressed markers in human follicular cells associated with competent oocytes. Hum Reprod. 2008, 23 (5): 1118-1127.View ArticlePubMedGoogle Scholar
- Robert C, Gagne D, Bousquet D, Barnes FL, Sirard MA: Differential display and suppressive subtractive hybridization used to identify granulosa cell messenger rna associated with bovine oocyte developmental competence. Biology of reproduction. 2001, 64 (6): 1812-1820.View ArticlePubMedGoogle Scholar
- Assidi M, Dufort I, Ali A, Hamel M, Algriany O, Dielemann S, Sirard MA: Identification of Potential Markers of Oocyte Competence Expressed in Bovine Cumulus Cells Matured with Follicle-Stimulating Hormone and/or Phorbol Myristate Acetate In Vitro. Biology of reproduction. 2008, 79 (2): 209-222.View ArticlePubMedGoogle Scholar
- NIA: National Institute on Aging (NIA/NIH): Laboratory of Genetics. 2010, Baltimore MD , USA, [http://lgsungrcnianihgov/ANOVA/2010]Google Scholar
- Assidi M, Montag M, Van Der Ven K, Sirard MA: Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study. Journal of assisted reproduction and genetics. 2011, 28 (2): 173-88.PubMed CentralView ArticlePubMedGoogle Scholar
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