Volume 15 Supplement 2

Abstracts from the 2nd International Genomic Medicine Conference (IGMC 2013)

Open Access

Analysis of correlations between zona pellucida birefringence and molecular markers of oocyte developmental competence

BMC Genomics201415(Suppl 2):P48

https://doi.org/10.1186/1471-2164-15-S2-P48

Published: 2 April 2014

Background

Human infertility is the incapacity of a couple to conceive after one year of unprotected sexual intercourse. Selection of the best gametes for subsequent steps of fertilization and embryo transfer was shown to be the crucial step in infertility treatment procedure [1, 2]. Oocyte selection using morphological criteria has been the gold standard method in assisted reproductive technologies (ART) clinics. Zona Pellucida (ZP) , a filamentous matrix of glycosylated glycoproteins surrounding the oocyte, is one of these morphological criteria of oocyte selection. In fact, ZP thickness and birefringence was reported to be positively correlated with higher ability of the oocyte to achieve successful pregnancy, but this selection approach has limitations in terms of accuracy, objectivity and constancy. Recent studies using OMICs approaches have identified key molecular markers in somatic cells (cumulus and/or granulosa cells) and follicular fluid that quantitatively and non-invasively predict the oocyte quality for better selection, higher pregnancy rates and efficient infertility treatment. These biomarkers could be a valuable reinforcement of the morphological selection criteria widely used in IVF clinics. In this context, this study was designed to study the relationship between some molecular predictors of oocyte quality found by our group and the conventional morphological parameters of oocyte quality. We expect to find a positive correlation between the ZP birefringence and molecular markers of oocyte competence. Such integrative strategy should lead to a powerful combined approach that will precisely predict the oocyte developmental potential, allowing therefore efficient infertility treatment and elective single embryo transfer (eSET).

Materials and methods

Seven (7) patients with informed consent were selected for ovarian stimulation, ICSI (intracytoplasmic sperm injection) and subsequent embryo transfer at day 3 at the IVF clinic of Bonn University Medical School. Cumulus-oocyte complex (COC) were retrieved 36 to 38 h after HCG administration and washed. While cumulus cells (CCs) of each oocyte were individually collected and put at -80°C for subsequent RNA extraction (PicoPure, Molecular Devices, CA), the metaphase II (MII) oocytes were completely denuded by hyaluronidase followed successively by automatic ZP birefringence assessment (Octax polairAide™, OCTAX Microscience GmbH, Altdorf, Germany); [3]) and ICSI one hour later. MII oocytes having an inner zona layer with reduced or asymmetrical birefringence were considered as Zona Bad (ZB). Conversely, oocytes with high and uniform birefringence were named Zona good (G)[3, 4] (Figure 1). After ICSI and individual culture, the two embryos having the two top ZP birefringence scores were transferred. Successful pregnancy evaluated by HCG test at day 14, and confirmed by ultrasons at week 5. Both ZP birefringence and pregnancy results were used to distinguish two groups of patients 1) High ZP birefringence and successful pregnancy (ZGP), versus 2) Low/irregular ZP birefringence and pregnancy failure (ZBNP). After RNA amplification (RiboAmpplus RNA Amplification kit, Molecular Devices), differentially expressed gene analysis in CCs of ZGP (8 CCs samples) versus ZBNP (3 CCs samples) was done by hybridization using two platforms: a custom- made microarray [57] as well as the OneArray chips (30K; Phalanx Biotech, Palo Alto, CA). Data analysis using National Institute on Aging (NIA) Array Analysis Tool (Baltimore, MD) [8] allowed the identification of both over-expressed and under-expressed gene lists (fold change ≥ 2; FDR=5%) of the ratio ZGP/ZBNP; and the identification of reliable markers of oocyte quality associated to ZP birefringence and pregnancy output. Selected gene markers were validated by QPCR.
Figure 1

Automatic scoring at 180 points of ZP of MII oocytes using the Octax PolarAIDE polarized microscopy (MTG 2010)

Results

Possible correlation between 7 biomarkers differentially expressed in the CCs of good quality oocytes [9] and the ZP birefringence was analyzed in order to assess if these two approaches (combination of ZP morphology and molecular markers) are additive, and hence able to strengthen the highly competent oocytes selection procedure. 32 and 50 candidate genes were respectively under-expressed and overexpressed in the ZGP compared to the ZBNP. Interestingly, three (3) positive biomarkers from the (ZGP vs ZGNP) comparison were also present in the overexpressed gene list of the (ZGP vs ZBNP). These biomarkers are PSMD6, CALM1 and NRP1. Surprisingly, this study demonstrated that most of the analyzed biomarkers were not significantly different between the two groups with failed pregnancy (ZGNP vs ZBNP) (Figure 2). Therefore and despite having two opposite ZP birefringence, few or no transcriptional differences were found between these two groups.
Figure 2

Schematic representation of the correlation (expressed in %) of significant gene biomarkers between the three morphological groups of ZB

Conclusions

Weak correlations between the 7 gene biomarkers of oocyte developmental potential and the ZP birefringence score. It looks that the ZP morphology is associated to a transcriptomic gene pattern that is not directly related to the developmental competence pathway. Further studies using larger lists of candidate markers are required to identify suitable genes that are highly correlated with the morphological criteria, and therefore able to reinforce the accuracy of oocyte selection. Together, the combination of these two approaches should offer a reliable prognostic tool of the best oocyte allowing efficient infertility treatment and successful pregnancy.

Authors’ Affiliations

(1)
Centre de Recherche en Biologie de la Reproduction, Laval University
(2)
Center of Excellence in Genomic Medicine Research, King Abdulaziz University
(3)
KACST Technology Innovation Center in Personalized Medicine, King Abdulaziz University
(4)
Department of Gynecological Endocrinology and Reproductive Medicine, Bonn University

References

  1. Balaban B, Urman B: Effect of oocyte morphology on embryo development and implantation. Reproductive biomedicine online. 2006, 12 (5): 608-615.View ArticlePubMedGoogle Scholar
  2. Krisher RL: The effect of oocyte quality on development. J Anim Sci. 2004, 82 (E-Suppl): E14-23.PubMedGoogle Scholar
  3. Montag M, Schimming T, Koster M, Zhou C, Dorn C, Rosing B, van der Ven H, Ven der Ven K: Oocyte zona birefringence intensity is associated with embryonic implantation potential in ICSI cycles. Reproductive biomedicine online. 2008, 16 (2): 239-244.View ArticlePubMedGoogle Scholar
  4. MTG: Octax PolarAIDE: Automatic assessment of oocyte quality and beyond. 2010Google Scholar
  5. Hamel M, Dufort I, Robert C, Gravel C, Leveille MC, Leader A, Sirard MA: Identification of differentially expressed markers in human follicular cells associated with competent oocytes. Hum Reprod. 2008, 23 (5): 1118-1127.View ArticlePubMedGoogle Scholar
  6. Robert C, Gagne D, Bousquet D, Barnes FL, Sirard MA: Differential display and suppressive subtractive hybridization used to identify granulosa cell messenger rna associated with bovine oocyte developmental competence. Biology of reproduction. 2001, 64 (6): 1812-1820.View ArticlePubMedGoogle Scholar
  7. Assidi M, Dufort I, Ali A, Hamel M, Algriany O, Dielemann S, Sirard MA: Identification of Potential Markers of Oocyte Competence Expressed in Bovine Cumulus Cells Matured with Follicle-Stimulating Hormone and/or Phorbol Myristate Acetate In Vitro. Biology of reproduction. 2008, 79 (2): 209-222.View ArticlePubMedGoogle Scholar
  8. NIA: National Institute on Aging (NIA/NIH): Laboratory of Genetics. 2010, Baltimore MD , USA, [http://lgsungrcnianihgov/ANOVA/2010]Google Scholar
  9. Assidi M, Montag M, Van Der Ven K, Sirard MA: Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study. Journal of assisted reproduction and genetics. 2011, 28 (2): 173-88.PubMed CentralView ArticlePubMedGoogle Scholar

Copyright

© Assidi et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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