Volume 15 Supplement 2
Initial characterization of drug resistant cancer stem cells isolated from primary brain tumors (astrocytoma) cell lines generated from Saudi patients
© Khan et al; licensee BioMed Central Ltd. 2014
Published: 2 April 2014
The Cancer Stem Cells (CSCs) hypothesis proposes that malignant brain tumours are organized into aberrant cell hierarchies where a subset of parent CSCs replicate asymmetrically and unlimitedly to produce differentiated daughter cell [1, 2]. These parent CSCs are highly adaptive and resistant to the chemotherapeutic drugs . Developing new drugs that target CSCs requires a comprehensive understanding of the pharmacogenomics behavior of these cells [4, 5]. Such understanding requires reliable in vitro and in vivo models that represent the beneficial patients. This project is set out to: i) Establish a collection of astrocytoma cell lines generated from Saudi patients, ii) Select drug resistant brain tumour CSCs, iii) Characterize drug resistant brain tumour CSCs individually, and iv) Deduce common features for drug resistant brain tumour CSCs.
Materials and methods
A range of methodologies will be applied including, tumour cell line derivation methods, the clonogenic selection assay, cytogenetics profiles, neuronal cancer stem cells characterisation assays and in vivo tumourgenic assays.
Optimisation of methods used to establish astrocytoma cell lines generated from Saudi patients was completed. To date two novel primary astrocytoma cell lines have been derived. Full clinical data related to the cell lines was retrieved. Currently, optimisation of the clonogenic selection assay and immunostaining for cancer stem cells markers are under progress.
Successful retrieval of primary astrocytoma cell lines was possible to accomplish. Important clinical information data relevant to the cell lines were obtained providing clear identification reference. Further work is required for the characterization of the drug resistant cancer stem cell component within these cell lines. Once completed, this research will assist in understanding some of the molecular mechanisms relevant to drug resistance, especially for patients in the Kingdom of Saudi Arabia.
- Dirks PB: Brain tumour stem cells: the undercurrents of human brain cancer and their relationship to neural stem cells. Philosophical Transactions of the Royal Society B: Biological Sciences. 2008, 363 (1489): 139-152.View ArticleGoogle Scholar
- Khoshnevisan A: An overview of therapeutic approaches to brain tumor stem cells. Medical journal of the Islamic Republic of Iran. 2012, 26 (1): 31-PubMed CentralPubMedGoogle Scholar
- Quezada C, Garrido W, Oyarzún C, Fernández K, et al: 5′ ectonucleotidase mediates multipledrug resistance in glioblastoma multiforme cells. Journal of cellular physiology. 2013, 228 (3): 602-608.View ArticlePubMedGoogle Scholar
- Maugeri-Saccà M, Vigneri P, De Maria R: Cancer stem cells and chemosensitivity. Clinical Cancer Research. 2011, 17 (15): 4942-4947.View ArticlePubMedGoogle Scholar
- Reya T, Morrison SJ, Clarke MF, Weissman IL: Stem cells, cancer, and cancer stem cells. Nature. 2001, 414 (6859): 105-111.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.