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BMC Genomics

Open Access

Adenosine kinase as a therapeutic target in traumatic optic neuropathy

  • Saif Ahmad1, 2Email author,
  • Nehal El-Sherbiny2, 3,
  • Ahmed El-Sherbini2,
  • Sadan Fulzele4 and
  • Gregory I Liou2
BMC Genomics201415(Suppl 2):P58

https://doi.org/10.1186/1471-2164-15-S2-P58

Published: 2 April 2014

Background

The purpose of this study is to understand the mechanism of traumatic optic neuropathy (TON) in order to prevent vision loss. Following traumatic insults to the optic nerve, retinal microglia cells are activated through MAP Kinase pathways and increased cytotoxic activity that causes retinal ganglion cell death [1]. Under stress condition, extracellular concentration of adenosine is likely to increase and activates an anti-inflammatory pathway through A2A adenosine receptor. But in TON, the accumulated extracellular adenosine is then transported intracellular through equilibrative nucleoside transporters (ENTs) which further gets converted into AMP by Adenosine kinase (AK), which results in low extracellular adenosine concentration. We have demonstrated that microglia activation and TNF-α release was inhibited by AK inhibitor. Based on these findings, we hypothesize that an imbalance in adenosine formation and metabolism in the retinal microglia participated by AK may contribute significantly to retinal complications in the setting of TON.

Materials and methods

Mice were anesthetized according to standard protocol and bilateral limbal conjunctival peritomy was performed posteriorly to the optic nerve in each mouse. Mice were treated with or without an AK inhibitor (AKI), ABT702 (25μg/kg; i. p.), every other day for 7 days. All retinas or eye ball were then harvested for protein, histology and RNA expression.

Results

We found that in TON, RGC number significantly decreased, Cleaved Caspase-3, Iba1, cd11b expression and TUNEL positive cells increased as compared with control eye (Fig 1 A-C). In a series of experiments with Western Blot, Immunohistology and Real-Time PCR, increased expression of TNF-α, Iba1, iNOS and IL-6 were shown in nerve crush model as compared with AKI treatment. It was also noted that administration of AKI showed no effect in the control.
Figure 1

A) H & E staining of TON eye section for RGC counting. B) Quantitative analysis of TUNEL-positive cells in the retinal cross section of TON and control. Immunohistochemical analysis of activated Caspase-3 in retinal section of TON vs. control. C) Immunohistochemical and Immunoblotting of microglial activation marker Iba-1 and CD11b expression in TON vs. control.

Conclusions

Based on our findings, we conclude that Adenosine Kinase inhibition may have potential role in regulating inflammatory mechanism in TON mouse model.

Authors’ Affiliations

(1)
Department of Biological Sciences, Rabegh College of Science and Arts, King Abdulaziz University
(2)
Department of Ophthalmology, Georgia Regents University
(3)
Faculty of Pharmacy, Mansoura University
(4)
Department of Orthopedics, Georgia Regents University

References

  1. Ahmad S, Fatteh N, El-Sherbiny N, Naime N, Ibrahim A, et al: Potential role of A2A adenosine receptor in traumatic optic neuropathy. J Neuroimmunology. 2013, 264: 54-64.View ArticleGoogle Scholar

Copyright

© Ahmad et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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