- Research article
- Open Access
The catalytic domains of thiamine triphosphatase and CyaB-like adenylyl cyclase define a novel superfamily of domains that bind organic phosphates
© Iyer and Aravind; licensee BioMed Central Ltd. 2002
- Received: 19 September 2002
- Accepted: 27 November 2002
- Published: 27 November 2002
The CyaB protein from Aeromonas hydrophila has been shown to possess adenylyl cyclase activity. While orthologs of this enzyme have been found in some bacteria and archaea, it shows no detectable relationship to the classical nucleotide cyclases. Furthermore, the actual biological functions of these proteins are not clearly understood because they are also present in organisms in which there is no evidence for cyclic nucleotide signaling.
We show that the CyaB like adenylyl cyclase and the mammalian thiamine triphosphatases define a novel superfamily of catalytic domains called the CYTH domain that is present in all three superkingdoms of life. Using multiple alignments and secondary structure predictions, we define the catalytic core of these enzymes to contain a novel α+β scaffold with 6 conserved acidic residues and 4 basic residues. Using contextual information obtained from the analysis of gene neighborhoods and domain fusions, we predict that members of this superfamily may play a central role in the interface between nucleotide and polyphosphate metabolism. Additionally, based on contextual information, we identify a novel domain (called CHAD) that is predicted to functionally interact with the CYTH domain-containing enzymes in bacteria and archaea. The CHAD is predicted to be an alpha helical domain, and contains conserved histidines that may be critical for its function.
The phyletic distribution of the CYTH domain suggests that it is an ancient enzymatic domain that was present in the Last Universal Common Ancestor and was involved in nucleotide or organic phosphate metabolism. Based on the conservation of catalytic residues, we predict that CYTH domains are likely to chelate two divalent cations, and exhibit a reaction mechanism that is dependent on two metal ions, analogous to nucleotide cyclases, polymerases and certain phosphoesterases. Our analysis also suggests that the experimentally characterized members of this superfamily, namely adenylyl cyclase and thiamine triphosphatase, are secondary derivatives of proteins that performed an ancient role in polyphosphate and nucleotide metabolism.
- Adenylyl Cyclase
- Adenylyl Cyclase Activity
- Organic Phosphate
- Aeromonas Hydrophila
- Yersinia Pestis
Organic phosphate compounds are the central metabolites of all biological systems [1, 2]. Some are the basic building blocks of nucleic acids, some like ATP and GTP, are additionally, cellular energy stores, others like cAMP or cGMP are messengers in signal transduction, and, yet others, such as FAD, NAD, thiamine phosphates and pyridoxal phosphate are cofactors for a range of enzymes [1, 2]. Protein domains belonging to a relatively small set of structural folds are known to bind or catalyze reactions that utilize these organic phosphate compounds (see SCOP database: http://scop.mrc-lmb.cam.ac.uk/scop/) [3, 4]. Several of these folds trace back to some of the earliest phases of the evolution of the protein world, and participate in a wide range of disparate biological functions in extant proteins [4, 5]. Some folds, such as the P-loop fold, the Rossmann fold and the Hsp70-like fold, have been well studied, and comprise mainly of dedicated nucleotide binding or hydrolyzing proteins [6–9]. Others, such as the palm-domain, which is found in adenylyl cyclases and various nucleic acid polymerases, belong to more generalized protein folds that contain representatives with diverse biochemical activities [4, 10, 11]. Current availability of extensive genome sequence data, allows one to identify less numerous, nevertheless biological important organic phosphate-binding domains that may have previous eluded detection. The identification of such domain superfamilies, containing enzymes with several different activities, often throws considerable light on their evolution, structure and catalytic mechanisms [4, 12].
The majority of previously known nucleotide cyclases belong to two major folds. The classical adenylyl cyclases, guanylyl cyclases and the GGDEF (diguanylate cyclase) domains share the catalytic palm domain with the family B DNA polymerases, reverse transcriptases, viral RNA dependent RNA polymerases and eukaryote-type primases [4, 13, 14]. The pathogenic adenylyl cyclases of several bacteria and the CyaA-like proteobacteria adenylyl cyclases are extremely divergent versions of the catalytic domain seen in the Pol-β family of nucleotidyl transferases  (also see SCOP database: http://scop.mrc-lmb.cam.ac.uk/scop/). While the catalytic domains of these two superfamilies have very different folds, they follow a similar reaction mechanism that is dependent on two Mg2+ ions coordinated by a cluster of acidic residues. However, the CyaB adenylyl cyclase, which was identified in the bacterium Aeromonas hydrophila is unrelated to any of these above superfamilies of enzymes . Though close relatives of this enzyme exist in some bacteria, like Yersinia pestis and Borrelia burgdorferi and the archaea, its antecedents or catalytic mechanism have not been understood. Using sensitive sequence profile comparison methods, we show that the CyaB-like adenylyl cyclases are homologs of the soluble mammalian thiamine triphosphatases , and they define a novel superfamily of enzymes that utilize ATP and other organic phosphates. We present evidence that a representative of this domain was present in the last common ancestor of all extant life forms. The primary biological function of these proteins appears to be related to polyphosphate and nucleotide metabolism. Cyclic AMP generation and thiamine triphosphate hydrolysis appear to be secondarily acquired activities. We also identify the potential active site- and substrate interacting-residues and postulate that these enzymes are likely to catalyze a two-metal ion dependent reaction on structural scaffold that is completely different from that seen in the other two superfamilies of adenylyl cyclases.
Identification of the CYTH domain
In order to understand the evolutionary affinities and provenance of the CyaB adenylyl cyclase from Aeromonas hydrophila, we carried out database searches using sensitive sequence profile analysis methods. As CyaB is a small protein with no detectable low complexity regions, we used it as a seed to initiate a PSI-BLAST search  (run to convergence, with expect-value for inclusion in profile = .01). The search resulted in the detection of its obvious orthologs from Yersinia and various archaea at significant expect (e) values ranging from from 3 × 10-43 to 8 × 10-5. The second iteration recovered proteins from more archaea, eukaryotes (e-value: 3 × 10-7), Clostridium (3 × 10-8) and Borrelia (6 × 10-8). Further iterations, run to convergence, recovered the soluble mammalian thiamine triphosphatases (3 × 10-4), and the N-terminal region of E. coli YgiF. At convergence, several bacterial proteins, that showed a conserved EXEXK (where X is any amino acid) characteristic of this family, and a region C-terminal to a P-loop like uridine kinase domain in plants were also recovered at borderline e-values (e value ~0.01 – 0.05). Reciprocal searches initiated with the E. coli YgiF protein (residues 1–200), not only recovered its bacterial orthologs, archaeal CyaB homologs and eukaryotic proteins, but also several others such as Bacillus subtilis YjbK, Methanosarcina Ma2350, and Mesorhizobium loti Mll4592 with e-values in the range of 10-4–10-6 upon first detection. Additionally, transitive searches initiated from the region C-terminal to the uridine kinase of the plants (49D11.13 from Oryza sativa, region: 250–410) recovered archaeal CyaB homologs confirming their relationship to with the other proteins detected in these searches. Regular expression searches with the conservation pattern found in these CyaB homologs also recovered the most of the members detected in the above-mentioned profile searches, but failed to recover any new candidates.
In all these searches, the alignments more or less spanned the entire length of the CyaB protein and typically contained the same set of conserved residues. The Gibbs sampling procedure revealed the presence of seven conserved motifs, with a probability of chance occurrence less than 10-14, in the search space comprising of the 70 or so proteins that were identified in the above searches as having this domain. We clustered these proteins using the BLASTCLUST program in several smaller clusters and prepared multiple alignments for the individual clusters and predicted secondary structure for these set using the PHD program. A nearly complete congruence was seen in the comparison of the secondary structures of the individual clusters. In many cases, the region of similarity to CyaB comprised the entire length of the target protein detected in these searches. However, in some cases it only comprised a part of the protein, with rest of the protein being made of other globular domains. These observations, taken together, suggested that CyaB and soluble mammalian thiamine triphosphatases define a novel superfamily of conserved domains, which may either occur by itself or in combination with other domains. We named this domain the CYTH (Cy aB, th iamine triphosphatase) domain after the two experimentally characterized proteins in which it is present.
Sequence conservation, structure and biochemical activities of the CYTH domain
The four conserved basic residues in the CYTH domain are most probably involved in the binding of acidic phosphate moieties of their substrates (Fig. 1). The conservation of these two sets of residues in the majority of CYTH domains suggests that most members of this group are likely to possess an activity dependent on two metal ions, with a preference for nucleotides or related phosphate-moiety-bearing substrates. The proposed biochemical activity, and the arrangement of predicted strands in the primary structure of the CYTH domain imply that the may adopt a barrel or sandwich-like configuration, with metal ions and the substrate bound in the central cavity. The only prominent exceptions to the basic conservation pattern of the CYTH domains are the versions found in the plant and Dictyostelium pyrimidine kinase homologs (Fig. 1, At1g26190-like). These versions lack 5 of the 6 conserved acidic residues, but retain 3 of the 4 conserved basic residues (Fig. 1). This leads to the prediction that these CYTH domains are catalytically inactive. However, as they retain the basic residues, they probably still bind the organic phosphate substrates, and function as regulatory domains that are linked to P-loop kinase domains.
Phyletic patterns, evolutionary history and potential biological functions of the CYTH domains
However, there are certain anomalies to this pattern. The CYTH domains are entirely absent from the small genomes of pathogenic bacteria such as Rickettsia and Chlamydia as well as some of the large genomes such as Deinococcus radiodurans. At least, a single copy of the CYTH domain is seen in most archaeal and eukaryotic genomes sampled to date, with the exception of Thermoplasma and the yeasts, where it is absent. This implies that the CYTH domain has been lost independently on a number of occasions in evolution. CyaB homologs from Aeromonas, Clostridium, Borrelia, and Ralstonia lie firmly (RELL Bootstrap >= 70%) within the archaeal and eukaryotic clusters, rather than with their bacterial counterparts (Fig. 2). These bacterial forms also share the unique sequence signature in the second strand with this group suggesting that they have been derived through horizontal transfer from different archaeal and eukaryotic sources (Fig. 1). In particular the CyaB homolog from Ralstonia groups very strongly with the animal versions and appears to be a recent horizontal transfer in this bacterium from the latter clade. The possibility of lateral transfer of Aeromonas CyaB from an archaeal source has been previously suggested, and is consistent with the enzyme being optimally functional under high temperature . There are 3 distinct CYTH domains in the euryarchaeon Methanosarcina acetivorans, in addition to the version which groups with the CYTH domains that are found, in single or duplicate copies, in other archaea and eukaryotes. These former versions, strongly group with CYTH domains from actinobacteria (Rell Boostrap 77%) to the exclusion of other lineage (Fig. 2). Furthermore, they share a fusion to a novel conserved domain with characteristic histidines (see below) with the actinobacterial versions. Thus they appear to have been transferred laterally from the actinobacteria into the Methanosarcina lineage followed by a small lineage specific expansion in the latter. Bacteriophages, like RB49, that contain a solo CYTH domain, which is closer to the version seen in its proteobacterial hosts, could have served as conduits for the lateral distribution of this domain.
In terms of conserved gene neighborhoods, CYTH-domain-encoding genes, like mll4592 from α-proteobacteria, are frequently found in the neighborhood of genes encoding solo CHADs (Fig. 3). Additionally, in Methanosarcina the CYTH-encoding genes are found in predicted operons or in the neighborhood of genes encoding exopolyphosphatase (PPX) and polyphosphate kinase (PPK). Furthermore, in Sulfolobus, a gene for a CHAD protein is in a predicted operon along with genes for thymidylate kinase and PPX. CHAD- and CTYH-encoding genes are also found in the neighborhood of PPK and PPX in Chlorobium tepidum and Geobacter metallireducens, respectively (Fig. 3). Genes for CYTH domains also co-occur in predicted operons with genes for another polyphosphate utilizing enzyme, the polyphosphate-dependent NAD kinase (PPNK), in certain cyanobacteria (eg. Prochlorococcus marinus) and Gram positive bacteria like Oceanobacillus iheyensis. Other potential connections are furnished by the co-occurrence of genes for CYTH-domain proteins with genes involved in with nucleoside polyphosphate metabolism. These include co-occurrence with the gene for adenosine tetraphosphatase (APAH; eg. in Magnetococcus sp.) and with genes encoding the YjbM-domain in Gram-positive bacteria. The YjbM domain, most often, occurs fused to pentaphosphate guanosine-3'-pyrophosphohydrolase (SpoT) and GTP pyrophosphokinase (RelA), suggesting a role for it in the metabolism of the stringent-response nucleoside polyphosphate.
These contextual connections are consistent with the participation of CYTH domains in organo-phosphate biochemistry, and circumstantially associate it with the metabolic network related to polyphosphates and nucleoside polyphospates (Fig. 3) . Specifically, PPK and PPX have been shown to, respectively, lengthen or shorten polyphosphate polymers . These two enzymes also appear to interact with the nucleotide metabolism of the cell. In particular PPK and PPNK can utilize Poly(P) to synthesize nucleoside polyphosphates, while PPK along with adenylate kinase can carry out polyphosphate-dependent phosphorylation AMP [33–35]. Hence, it is likely that the CYTH domain proteins participate directly in this biochemical network along with these proteins. One possibility is that the CYTH domains utilize polyphosphates to synthesize different organo-phosphate derivatives including nucleotides. Alternatively, they could also function as phosphoesterases that hydrolyze particular nucleoside polyphosphates. These leads could aid further experimental investigations on the CYTH domain that might help in uncovering ancient, as-yet-unexplored links between polyphosphate and nucleotide metabolism.
Finally, at least in some lineages, the CYTH domain proteins may have been secondarily recruited for other functions. The CyaB protein may represent one such case where after the original transfer from an archaeon into the proteobacterial lineage it may have acquire the novel function as an adenylyl cyclase. However, it is entirely possible that even in this case the adenylyl cyclase activity is secondary to some other uncharacterized metabolic activity. The vertebrate soluble thiamine triphosphatase has undergone accelerated divergence, as it is present on a long branch in the phylogenetic tree (Fig. 2). ThTPase is also unusual in lacking the 3rd conserved acidic domain of the CYTH domain. Hence, it may represent a case of relatively recent acquisition of a new catalytic activity.
We show that Aeromonas adenylyl cyclase CyaB and thiamine triphosphatase define a novel superfamily of catalytic domains that act on nucleotides and organo-phosphate substrates. These domains are widely distributed in all the 3 superkingdoms of life and can be traced back to the last ancestor of all life forms. We identify 6 conserved acidic residues, that are likely to form the active site of these enzymes, and 4 conserved basic residues, that may participate in interactions with phosphate-moiety-containing substrates. We postulate that these enzymes are likely to chelate 2 divalent cations and are likely follow a bimetal reaction mechanism similar to what has been proposed for nucleotide cyclases, nucleic acid polymerases, or certain phosphoesterases such as those of the HD and DHH superfamilies. A version of the CYTH domain, which is fused to the catalytic domain of nucleotide kinases, lacks the predicted catalytic residues, and probably function as an allosteric regulatory domain. Additionally, we detected a novel domain, termed CHAD, which occurs fused to the CYTH domain or is encoded by genes occurring in the same operon as those encoding CYTH domains. CHAD contains conserved histidines that are predicted to either chelate metals or serve as phosphoacceptors. Based on phyletic distribution and contextual information, we conclude that these enzymes may play a critical role in the interface between nucleotide and polyphosphate metabolism.
The non-redundant (NR) database of protein sequences (National Center for Biotechnology Information, NIH, Bethesda) was searched using the BLASTP and PSI-BLAST programs . Profile searches using the PSI-BLAST program were conducted either with a single sequence or an alignment used as the query, with a profile inclusion expectation (E) value threshold of 0.01 and were iterated until convergence. Additionally, hidden Markov model based searches using a multiple alignment of known members were run using the HMMER2 package . The Gibbs sampling procedure, as implemented in the MACAW program was used to detect and evaluate statistically significant conserved motifs . Multiple alignments were constructed using the T_Coffee program , followed by manual correction based on the PSI-BLAST results. Protein secondary structure was predicted using a multiple alignment as the input for the JPRED and PHD programs [39, 40]. Preliminary clustering of proteins was done using the BLASTCLUST program with empirically determined length and score threshold cut off values (For documentation see ftp://ftp.ncbi.nih.gov/blast/documents/README.bcl). Phylogenetic analysis was performed using the neighbor joining or least square method followed by local rearrangements using the maximum likelihood algorithm to predict the most likely tree. The robustness of tree topology was assessed with 10,000 Resampling of Estimated Log Likelihoods (RELL) bootstrap replicates. The MOLPHY and Phylip software packages were used for phylogenetic analyses [41, 42].
The species abbreviations used in the alignments are: Aehy: Aeromonas hydrophila, Af: Archaeoglobus fulgidus, Aga: Anopheles gambiae, Ana: Anabaena sp. PCC 7120, Ap: Aeropyrum pernix, At: Arabidopsis thaliana, Atu: Agrobacterium tumefaciens, Ban: Bacillus anthracis, Bb: Borrelia burgdorferi, Bha: Bacillus halodurans, BPRB49: Bacteriophage RB49, Bs: Bacillus subtilis, Cac: Clostridium acetobutylicum, Cau: Chloroflexus aurantiacus, Ccr: Caulobacter crescentus, Ce: Caenorhabditis elegans, Chte: Chlorobium tepidum, Cgl: Corynebacterium glutamicum, Cpe: Clostridium perfringens, Ddi: Dictyostelium discoideum, Dm: Drosophila melanogaster, Ec: Escherichia coli, Gme: Geobacter metallireducens, Hi: Haemophilus influenzae, Hs: Homo sapiens, Hsp: Halobacterium sp., Lin: Listeria innocua, Lla: Lactococcus lactis, Lmo: Listeria monocytogenes, Mac: Methanosarcina acetivorans, Mcsp: Magnetococcus sp. Mfas: Macaca fascicularis, Mjan: Methanococcus jannaschii, Mka: Methanopyrus kandleri, Mlo: Mesorhizobium loti, Mma: Methanosarcina mazei, Mta: Methanothermobacter thermautotrophicus, Mtu: Mycobacterium tuberculosis, Nm: Neisseria meningitidis, Oih: Oceanobacillus iheyensis, Osa: Oryza sativa, Pa: Pyrococcus abyssi, Pae: Pseudomonas aeruginosa, Pfu: Pyrococcus furiosus, Ph: Pyrococcus horikoshii, Pmar: Prochlorococcus marinus, Pmu: Pasteurella multocida, Pyae: Pyrobaculum aerophilum, Rsol: Ralstonia solanacearum, Sa: Staphylococcus aureus, Scoe: Streptomyces coelicolor, Sen: Salmonella enterica, Sme: Sinorhizobium meliloti, Spn: Streptococcus pneumoniae, Spy: Streptococcus pyogenes, Sso: Sulfolobus solfataricus, Ssp: Synechocystis sp. PCC 6803, Sst: Sulfolobus tokodaii, StLT2: Salmonella typhimurium LT2, Vch: Vibrio cholerae, Xaxo: Xanthomonas axonopodis, Xca: Xanthomonas campestris, Xfa: Xylella fastidiosa, Ype: Yersinia pestis.
- Stryer L: Biochemistry. 1995, Newyork, NY: W H Freeman and Co, FourthGoogle Scholar
- Nelson DL, Cox MM: Lehninger Principles of Biochemistry. 2000, Worth Publishers IncGoogle Scholar
- Murzin AG, Brenner SE, Hubbard T, Chothia C: SCOP: a structural classification of proteins database for the investigation of sequences and structures. J Mol Biol. 1995, 247: 536-540. 10.1006/jmbi.1995.0159.PubMedGoogle Scholar
- Aravind L, Mazumder R, Vasudevan S, Koonin EV: Trends in protein evolution inferred from sequence and structure analysis. Curr Opin Struct Biol. 2002, 12: 392-399. 10.1016/S0959-440X(02)00334-2.View ArticlePubMedGoogle Scholar
- Mushegian AR, Koonin EV: A minimal gene set for cellular life derived by comparison of complete bacterial genomes. Proc Natl Acad Sci U S A. 1996, 93: 10268-10273. 10.1073/pnas.93.19.10268.PubMed CentralView ArticlePubMedGoogle Scholar
- Saraste M, Sibbald PR, Wittinghofer A: The P-loop – a common motif in ATP- and GTP-binding proteins. Trends Biochem Sci. 1990, 15: 430-434. 10.1016/0968-0004(90)90281-F.View ArticlePubMedGoogle Scholar
- Gorbalenya AE, Koonin EV: Superfamily of UvrA-related NTP-binding proteins. Implications for rational classification of recombination/repair systems. J Mol Biol. 1990, 213: 583-591.View ArticlePubMedGoogle Scholar
- Vetter IR, Wittinghofer A: Nucleoside triphosphate-binding proteins: different scaffolds to achieve phosphoryl transfer. Q Rev Biophys. 1999, 32: 1-56. 10.1017/S0033583599003480.View ArticlePubMedGoogle Scholar
- Bork P, Sander C, Valencia A: An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin, and hsp70 heat shock proteins. Proc Natl Acad Sci U S A. 1992, 89: 7290-7294.PubMed CentralView ArticlePubMedGoogle Scholar
- Artymiuk PJ, Poirrette AR, Rice DW, Willett P: A polymerase I palm in adenylyl cyclase?. Nature. 1997, 388: 33-34. 10.1038/40310.View ArticlePubMedGoogle Scholar
- Murzin AG: How far divergent evolution goes in proteins. Curr Opin Struct Biol. 1998, 8: 380-387. 10.1016/S0959-440X(98)80073-0.View ArticlePubMedGoogle Scholar
- Leipe DD, Wolf YI, Koonin EV, Aravind L: Classification and evolution of P-loop GTPases and related ATPases. J Mol Biol. 2002, 317: 41-72. 10.1006/jmbi.2001.5378.View ArticlePubMedGoogle Scholar
- Pei J, Grishin NV: GGDEF domain is homologous to adenylyl cyclase. Proteins. 2001, 42: 210-216. 10.1002/1097-0134(20010201)42:2<210::AID-PROT80>3.0.CO;2-8.View ArticlePubMedGoogle Scholar
- Koonin EV, Wolf YI, Kondrashov AS, Aravind L: Bacterial homologs of the small subunit of eukaryotic DNA primase. J Mol Microbiol Biotechnol. 2000, 2: 509-512.PubMedGoogle Scholar
- Aravind L, Koonin EV: DNA polymerase beta-like nucleotidyltransferase superfamily: identification of three new families, classification and evolutionary history. Nucleic Acids Res. 1999, 27: 1609-1618. 10.1093/nar/27.7.1609.PubMed CentralView ArticlePubMedGoogle Scholar
- Sismeiro O, Trotot P, Biville F, Vivares C, Danchin A: Aeromonas hydrophila adenylyl cyclase 2: a new class of adenylyl cyclases with thermophilic properties and sequence similarities to proteins from hyperthermophilic archaebacteria. J Bacteriol. 1998, 180: 3339-3344.PubMed CentralPubMedGoogle Scholar
- Lakaye B, Makarchikov AF, Antunes AF, Zorzi W, Coumans B, De Pauw E, Wins P, Grisar T, Bettendorff L: Molecular characterization of a specific thiamine triphosphatase widely expressed in mammalian tissues. J Biol Chem. 2002, 277: 13771-13777. 10.1074/jbc.M111241200.View ArticlePubMedGoogle Scholar
- Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25: 3389-402. 10.1093/nar/25.17.3389.PubMed CentralView ArticlePubMedGoogle Scholar
- Aravind L, Koonin EV: The HD domain defines a new superfamily of metal-dependent phosphohydrolases. Trends Biochem Sci. 1998, 23: 469-472. 10.1016/S0968-0004(98)01293-6.View ArticlePubMedGoogle Scholar
- Aravind L, Koonin EV: A novel family of predicted phosphoesterases includes Drosophila prune protein and bacterial RecJ exonuclease. Trends Biochem Sci. 1998, 23: 17-19. 10.1016/S0968-0004(97)01162-6.View ArticlePubMedGoogle Scholar
- Leipe DD, Aravind L, Grishin NV, Koonin EV: The bacterial replicative helicase DnaB evolved from a RecA duplication. Genome Res. 2000, 10: 5-16.PubMedGoogle Scholar
- Wolf YI, Aravind L, Grishin NV, Koonin EV: Evolution of aminoacyl-tRNA synthetases – analysis of unique domain architectures and phylogenetic trees reveals a complex history of horizontal gene transfer events. Genome Res. 1999, 9: 689-710.PubMedGoogle Scholar
- Anantharaman V, Koonin EV, Aravind L: Comparative genomics and evolution of proteins involved in RNA metabolism. Nucleic Acids Res. 2002,Google Scholar
- Ponting CP, Aravind L, Schultz J, Bork P, Koonin EV: Eukaryotic signalling domain homologues in archaea and bacteria. Ancient ancestry and horizontal gene transfer. J Mol Biol. 1999, 289: 729-745. 10.1006/jmbi.1999.2827.View ArticlePubMedGoogle Scholar
- Schultz JE, Klumpp S: Cyclic GMP in lower forms. Adv Pharmacol. 1994, 26: 285-303.View ArticlePubMedGoogle Scholar
- Huynen M, Snel B, Lathe W, Bork P: Predicting protein function by genomic context: quantitative evaluation and qualitative inferences. Genome Res. 2000, 10: 1204-1210. 10.1101/gr.10.8.1204.PubMed CentralView ArticlePubMedGoogle Scholar
- Iyer LM, Koonin EV, Aravind L: Classification and evolutionary history of the single-strand annealing proteins, RecT, Redbeta, ERF and RAD52. BMC Genomics. 2002, 3: 8-10.1186/1471-2164-3-8.PubMed CentralView ArticlePubMedGoogle Scholar
- Aravind L: Guilt by association: contextual information in genome analysis. Genome Res. 2000, 10: 1074-1077. 10.1101/gr.10.8.1074.View ArticlePubMedGoogle Scholar
- Makarova KS, Aravind L, Grishin NV, Rogozin IB, Koonin EV: A DNA repair system specific for thermophilic Archaea and bacteria predicted by genomic context analysis. Nucleic Acids Res. 2002, 30: 482-496. 10.1093/nar/30.2.482.PubMed CentralView ArticlePubMedGoogle Scholar
- Dandekar T, Snel B, Huynen M, Bork P: Conservation of gene order: a fingerprint of proteins that physically interact. Trends Biochem Sci. 1998, 23: 324-328. 10.1016/S0968-0004(98)01274-2.View ArticlePubMedGoogle Scholar
- Wolf YI, Rogozin IB, Kondrashov AS, Koonin EV: Genome alignment, evolution of prokaryotic genome organization, and prediction of gene function using genomic context. Genome Res. 2001, 11: 356-372. 10.1101/gr.GR-1619R.View ArticlePubMedGoogle Scholar
- Kornberg A, Rao NN, Ault-Riche D: Inorganic polyphosphate: a molecule of many functions. Annu Rev Biochem. 1999, 68: 89-125. 10.1146/annurev.biochem.68.1.89.View ArticlePubMedGoogle Scholar
- Shiba T, Tsutsumi K, Ishige K, Noguchi T: Inorganic polyphosphate and polyphosphate kinase: their novel biological functions and applications. Biochemistry (Mosc). 2000, 65: 315-323.Google Scholar
- Ishige K, Noguchi T: Polyphosphate:AMP phosphotransferase and polyphosphate:ADP phosphotransferase activities of Pseudomonas aeruginosa. Biochem Biophys Res Commun. 2001, 281: 821-826. 10.1006/bbrc.2001.4415.View ArticlePubMedGoogle Scholar
- Ishige K, Noguchi T: Inorganic polyphosphate kinase and adenylate kinase participate in the polyphosphate:AMP phosphotransferase activity of Escherichia coli. Proc Natl Acad Sci U S A. 2000, 97: 14168-71. 10.1073/pnas.011518098.PubMed CentralView ArticlePubMedGoogle Scholar
- Eddy SR: Profile hidden Markov models. Bioinformatics. 1998, 14: 755-63. 10.1093/bioinformatics/14.9.755.View ArticlePubMedGoogle Scholar
- Schuler GD, Altschul SF, Lipman DJ: A workbench for multiple alignment construction and analysis. Proteins. 1991, 9: 180-190.View ArticlePubMedGoogle Scholar
- Notredame C, Higgins DG, Heringa J: T-Coffee: A novel method for fast and accurate multiple sequence alignment. J Mol Biol. 2000, 302: 205-17. 10.1006/jmbi.2000.4042.View ArticlePubMedGoogle Scholar
- Rost B, Sander C: Prediction of protein secondary structure at better than 70% accuracy. J Mol Biol. 1993, 232: 584-99. 10.1006/jmbi.1993.1413.View ArticlePubMedGoogle Scholar
- Cuff JA, Clamp ME, Siddiqui AS, Finlay M, Barton GJ: JPred: a consensus secondary structure prediction server. Bioinformatics. 1998, 14: 892-893. 10.1093/bioinformatics/14.10.892.View ArticlePubMedGoogle Scholar
- Felsenstein J: Inferring phylogenies from protein sequences by parsimony, distance, and likelihood methods. Methods Enzymol. 1996, 266: 418-27.View ArticlePubMedGoogle Scholar
- Hasegawa M, Kishino H, Saitou N: On the maximum likelihood method in molecular phylogenetics. J Mol Evol. 1991, 32: 443-5.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.