The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae
- Roy Gross1Email author,
- Carlos A Guzman2Email author,
- Mohammed Sebaihia3,
- Vítor AP Martins dos Santos4,
- Dietmar H Pieper5,
- Ralf Koebnik6,
- Melanie Lechner1,
- Daniela Bartels7, 13,
- Jens Buhrmester8, 14,
- Jomuna V Choudhuri7, 15,
- Thomas Ebensen2,
- Lars Gaigalat8,
- Stefanie Herrmann9,
- Amit N Khachane4,
- Christof Larisch7,
- Stefanie Link1,
- Burkhard Linke7,
- Folker Meyer7, 13,
- Sascha Mormann8,
- Diana Nakunst8,
- Christian Rückert8,
- Susanne Schneiker-Bekel7,
- Kai Schulze2,
- Frank-Jörg Vorhölter8,
- Tetyana Yevsa2,
- Jacquelyn T Engle10,
- William E Goldman11,
- Alfred Pühler8,
- Ulf B Göbel9,
- Alexander Goesmann7,
- Helmut Blöcker12,
- Olaf Kaiser†7, 14 and
- Rosa Martinez-Arias†12
© Gross et al; licensee BioMed Central Ltd. 2008
Received: 02 February 2008
Accepted: 30 September 2008
Published: 30 September 2008
Bordetella petrii is the only environmental species hitherto found among the otherwise host-restricted and pathogenic members of the genus Bordetella. Phylogenetically, it connects the pathogenic Bordetellae and environmental bacteria of the genera Achromobacter and Alcaligenes, which are opportunistic pathogens. B. petrii strains have been isolated from very different environmental niches, including river sediment, polluted soil, marine sponges and a grass root. Recently, clinical isolates associated with bone degenerative disease or cystic fibrosis have also been described.
In this manuscript we present the results of the analysis of the completely annotated genome sequence of the B. petrii strain DSMZ12804. B. petrii has a mosaic genome of 5,287,950 bp harboring numerous mobile genetic elements, including seven large genomic islands. Four of them are highly related to the clc element of Pseudomonas knackmussii B13, which encodes genes involved in the degradation of aromatics. Though being an environmental isolate, the sequenced B. petrii strain also encodes proteins related to virulence factors of the pathogenic Bordetellae, including the filamentous hemagglutinin, which is a major colonization factor of B. pertussis, and the master virulence regulator BvgAS. However, it lacks all known toxins of the pathogenic Bordetellae.
The genomic analysis suggests that B. petrii represents an evolutionary link between free-living environmental bacteria and the host-restricted obligate pathogenic Bordetellae. Its remarkable metabolic versatility may enable B. petrii to thrive in very different ecological niches.
The genus Bordetella comprises several human and animal pathogens and nine species are currently described [see Additional file 1]. Human-restricted B. pertussis and B. parapertussis cause whooping cough, whereas B. bronchiseptica and B. avium are responsible for respiratory infections in many mammals and birds, respectively [1–3]. Despite the availability of vaccines, there are still 300,000 deaths/year caused by B. pertussis and significant economic losses associated with infections in poultry and cattle. The genomes of these Bordetellae were recently sequenced and analyzed [4, 5]. The genome sequence of B. avium revealed that it is quite divergent from the mammalian pathogens. In contrast, the genomes of the human pathogens B. pertussis and B. parapertussis showed that they are independent derivatives of B. bronchiseptica-like ancestors. They both underwent significant gene loss, probably mediated by insertion sequence (IS) elements, during the process of host adaptation, as indicated by their smaller genome sizes compared to that of B. bronchiseptica and the presence of many pseudogenes. The evolution of human-adapted species is characterized by massive genome reduction, lack of horizontal acquisition of genetic material and significant differences in virulence gene expression among species [4, 6].
The type strain of the only environmental species of the genus, B. petrii, was isolated from a dechlorinating bioreactor enriched by river sediment . B. petrii strains were repeatedly found in environmental samples, e.g. microbial consortia degrading aromatic compounds [8, 9]. Recently, two B. petrii strains were isolated from patients suffering from mandibular osteomyelitis and chronic suppurative mastoiditis [10, 11]. Bacteria closely related to B. petrii were also found in cystic fibrosis patients . Moreover, B. petrii strains were also isolated from marine sponges and a grass root consortium [13, 14]. Phylogenetic analysis shows that the Bordetellae are closely related to Achromobacter and Alcaligenes [see Additional file 1] [2, 7]. These environmental organisms occasionally cause severe nosocomial infections and they are also isolated from patients with cystic fibrosis [15, 16]. In addition to their medical relevance, these microorganisms also have an intrinsic biotechnological interest, because they show a remarkable capacity to degrade aromatic compounds and detoxify heavy metals [17, 18].
Since B. petrii exhibits properties of host-associated and environmental bacteria, it is important to perform a genomic analysis of this bacterium in order to understand the genetic basis of its versatility. The B. petrii genome provides insights into the evolutionary history of the Bordetellae, acting also as a spyglass to the Alcaligenes/Achromobacter group for which, despite their biotechnological potential, no sequenced genomes are available yet.
Results and discussion
Genome features and comparative analysis
General features of the Bordetella genomes
GC content (%)
Average gene size (bp)
IS3-family and others
Major features of the B. petrii genomic islands
Genomic islands (CDS)
Coordinates (size in bp)
No integrase and direct repeats (DR)
Capsular polysaccharide genes
Metabolism of phthalate and protocatechuate, urea amidohydrolase
clc-like, integrase, DR (3' end of tRNAGly)
Metabolism of an unknown aromatic compound (Bpet1116-1123)
clc-like, integrase, DR (3' end of tRNAGly)
Metabolism of benzoate, benzylalcohol, 3-hydroxybenzoate, putative monooxygenase (Bpet1330)
Putative (chloro)phenol monooxygenase (Bpet1331)
nitrile hydratase (Bpet1415-1416)
Almost identical to the clc element, integrase, DR (3' end of tRNAGly)
Metabolism of chlorocatechol, anthranilate
Short chain dehydrogenase (Bpet1512)
Tn4371-like, integrase, (T)6 DR
Integrase, features of conjugative transposons
Metabolism of chlorocatechol (2 gene clusters), tetrachlorobenzene
4 glutathione S-transferase (Bpet3724-3727)
clc-like, integrase, DR (3' end of tRNAGly)
Multidrug efflux pump
Iron transport system.
Integrase, DR (3' end of tRNAPhe), features of conjugative transposons
Several heavy metal resistance systems
Mobile genetic elements – a mosaic genome
The remaining GIs (GI4, GI5 and GI7) encode a conjugal transfer system similar to the type IV secretion systems associated with plasmids. GI4 is highly similar to Tn4371 of Ralstonia eutropha A5/Cupriavidus oxalaticus, a 55-kb conjugative transposon that carries genes involved in the degradation of biphenyl or 4-chlorobiphenyl . The features of all GIs are summarized in Table 2.
It has been previously reported that the chromosomes of B. pertussis and B. parapertussis carry a large number of IS elements (261 and 112, respectively). These IS elements are responsible for the extensive shuffling and deletions in their genomes . In contrast, B. bronchiseptica and B. avium carry none. B. petrii carries 105 IS elements, most of which belong to the IS3-family. Interestingly, there are six copies of IS1663 which was found in B. pertussis and one truncated copy of IS1001 (Bpet1198) which was found in B. parapertussis. The vast majority (66%) of the B. petrii IS are located within GIs, whereas 34% are randomly dispersed around the chromosome. B. petrii also carries two intact prophages (phage 1, Bpet0942-1008; phage 2, Bpet4384-4432) (Figure 1), and phage-like particles were indeed observed by electron microscopy of the sequenced strain . Phage 1 is inserted at the 3'end of a gene encoding a tRNAcys, while phage 2 is related to bacteriophage Mu. In addition, there are several regions on the B. petrii genome which may encode prophage remnants (Figure 1). In contrast, B. avium carries three and B. bronchiseptica carries four prophages, however, none of them is related to the B. petrii phages.
The GIs (GI1-GI7), IS elements and prophages represent 22% of the B. petrii genome. This highly mosaic genome structure reflects extensive DNA exchange and provides first evidence for a considerable horizontal gene transfer-driven evolution in the genus Bordetella.
Metabolism of B. petrii – genomic basis for metabolic versatility
Gluconate is an intermediate in the degradation of sugar acids and sugar derivatives, which are components of more complex molecules, such as plant materials and secondary metabolites. The genome analysis of B. petrii suggests that this microorganism can also utilize gluconate via a variant of the Entner-Doudoroff (ED) pathway, which is only found in few bacteria, such as Rhodobacter sphaeroides, but absent in all the other sequenced Bordetellae . In this pathway, gluconate is first transformed by a gluconate dehydratase (Bpet3300) to 2-dehydro-3-deoxy-D-gluconate, which is then imported into the cytoplasm via 2-dehydro-3-deoxy-D-gluconate permease (Bpet1985) and phosphorylated to 2-dehydro-3-deoxy-D-gluconate-6-phosphate by a 2-dehydro-3-deoxy-D-gluconate kinase (KdgK, Bpet0876). This compound is then converted to glyceraldehyde 3-phosphate and pyruvate by the action of 2-dehydro-3-deoxy-D-gluconate-6-phosphate aldolase (KdgA, Bpet0875). A IclR family transcription regulator (Bpet0874) is present immediately upstream of the kdgA and kdgK genes suggesting that this variant ED pathway may be inducible by this regulator depending on the availability of the external carbon source. In this regard, it is surprising that although a periplasmic glucose dehydrogenase (gcd, Bpet4644), which converts glucose to gluconate, is encoded in the genome, B. petrii is unable to metabolize glucose .
Interestingly, B. petrii encodes a protein (Bpet0241) with significant similarity to pectate lyases, extracellular enzymes secreted by phytopathogens, such as Erwinia carotovora, which catalyze the cleavage of pectate [24–26]. The reaction products are channeled into the variant ED pathway . In agreement with this, the determinants for two other important enzymes of these pathways, 2-deoxy-D-gluconate 3-dehydrogenase (Bpet0046, Bpet1132) and altronate hydrolase (Bpet0414) are present, indicating that B. petrii uses plant cell-wall constituents as nutrient sources, which is in line with the isolation of a B. petrii strain from a plant root consortium . The presence of a cyanate transporter (Bpet3878) and a cyanate lyase (Bpet3621) catalyzing the decomposition of cyanate into ammonia and bicarbonate, suggests that B. petrii is not only able to cope with the toxicity of environmental cyanate, but like E. coli may use it as a nitrogen source . A striking difference between B. petrii and the other Bordetellae is that it has a facultative anaerobic energy metabolism and can utilize nitrate as the terminal electron acceptor during anaerobic respiration . Accordingly, on its core genome B. petrii encodes the factors (nitrate-, nitrite-, and nitrous oxide reductases) necessary to carry out complete denitrification from nitrate to N2. Interestingly, only some of the respective genes are present in the pathogenic Bordetellae. B. bronchiseptica appears to encode a functional nitrate reductase, while B. parapertussis has a truncated napD gene probably leading to a non-functional nitrate reductase, and B. pertussis does not encode genes involved in denitrification at all.
Metabolic talents of B. petrii – degradation of aromatic compounds
A prominent feature is the presence of a surprisingly large number of genes coding for enzymes of the chloroaromatic metabolism. One complete set of chlorocatechol pathway genes (Bpet1533-1538) is localized on GI3 highly related to the clc element of Pseudomonas knackmussii B13. A second set of chlorocatechol pathway genes (Bpet3747-3752) is located on GI5 and shows high similarity to genes identified in the 1,2,4-trichlorobenzene degrading Pseudomonas strain P51 . A third possible chlorocatechol gene cluster encoding proteins only distantly related to those previously characterized is also located on GI5. This multiplicity of chlorocatechol gene clusters probably endows this strain with the ability to effectively metabolize a broad range of differently substituted chlorocatechols, providing thereby a valuable competitive advantage to thrive in polluted environments [30–32].
On GI5, chlorocatechol genes are preceded by a gene cluster (Bpet3738-3742) encoding a chlorobenzene dioxygenase and chlorobenzene dihydrodiol dehydrogenase, highly homologous to those of the 1,2,4-trichlorobenzene degraders Cupriavidus strain PS12 and Pseudomonas strain P51 [33, 34]. The absence of any linked extradiol dioxygenase encoding gene suggests that similarly to what is observed in the strains PS12 and P51, this cluster has specifically evolved for the transformation of chlorobenzenes [29, 30, 35].
B. petrii also harbors four different central pathways for aromatic metabolism via dihydroxylated central intermediates, the gentisate (Bpet0516-0517 and Bpet1429-1430, the latter located on GI2), the homogentisate (Bpet 4016–4017) and the catechol and protocatechuate branch of the 3-oxoadipate pathway (see below). Such versatility is similar to that observed in bacteria well known for their high metabolic capabilities, such as P. putida KT2440 and Cupriavidus necator . Only genes encoding enzymes of the homogentisate pathway were detected in all other sequenced Bordetellae, and those involved in the gentisate pathway are only present in B. avium.
Various natural aromatic compounds are known to be degraded via either the catechol or the protocatechuate branch of the 3-oxoadipate pathway, which thus plays a key role in bacterial aromatic catabolism. This pathway is widespread in Proteobacteria, however, all previously sequenced Bordetella strains are devoid of complete pathways and only a catIJCD cluster (Bpet4528-4532) is present as a remnant. In B. petrii, complete pathways are formed by genes of the protocatechuate branch (Bpet0260-0254) located in a genomic region with a quite low GC content, but lacking other typical features of a genomic island (named GI in Table 2 and in Additional file 3), and two clusters localized on GI2, encoding the enzymes of the catechol branch. We believe this to be the first example of natural horizontal transfer of this central aromatic metabolic pathway. Notably, also peripheral pathways for funneling benzoate (Bpet1396-1399) and benzylalcohol (Bpet1387-1388) to catechol are located on GI2. A peripheral pathway for channeling phthalate to protocatechuate (Bpet0248-0252) is located on GI1. The low number of peripheral routes contrasts with the situation in other Proteobacteria, such as B. xenovorans LB400, where five substrates are degraded via protocatechuate .
A strategy of aerobic degradation of aromatics alternative to the metabolism via dihydroxylated intermediates is initiated by CoA ligases [38, 39]. B. petrii carries gene clusters required for the metabolism of all three substrates, which are known to be degraded by such a strategy (Bpet0549-0554 for anthranilate metabolism and Bpet1924-1935 for phenylacetate metabolism, which are present in other Bordetellae, and Bpet3568-3575 for benzoate metabolism). As degradation via CoA derivatives needs less oxygen molecules compared to degradation via dihydroxylated intermediates, this may provide B. petrii with a selective advantage to also thrive in oxygen limited environments. For an overview about the aromatic degradation capabilities of B. petrii see Figure 5 and Additional file 3.
"Virulence factors" of B. petrii and host-restricted Bordetellae
Pathogenic Bordetellae produce many surface associated or secreted virulence factors . B. petrii does not code for the well-characterized toxins of classical Bordetellae (i.e., pertussis toxin, adenylate cyclase toxin and dermonecrotic toxin). However, it produces a low amount (22–53 nM/OD) of the non-protein tracheal cytotoxin (TCT), a spontaneously released muramylpeptide that disrupts epithelia . This expression range is comparable to the expression levels observed for the broad host range pathogen B. bronchiseptica.
Autotransporter or type V secretion proteins play a major role in Bordetella virulence . B. bronchiseptica encodes 21 autotransporter proteins, whereas B. petrii encodes only four (Bpet0190, Bpet0193, Bpet3980, Bpet4156) . Two of them (Bpet0190 and Bpet0193) are paralogous and show significant homology to the putative serine protease autotransporter BB2301 of B. bronchiseptica, whereas the other two do not show significant similarity to any database entry. Pathogenic Bordetellae also encode a two-partner secretion system which allows secretion of the colonization factor filamentous hemagglutinin (FhaB) . This protein requires the FhaC protein for translocation across the outer membrane. B. petrii encodes proteins related to FhaB and FhaC (Bpet600-601). B. petrii FhaB has 3039 amino acids and its N-terminus, which is required for export, is well-conserved with respect to pathogenic Bordetellae. However, the overall similarity with FhaB of pathogenic Bordetellae is very low, being more similar to putative secreted proteins of plant pathogens (e.g., Ralstonia, Xylella and Burkholderia). This suggests that the fha genes found in B. petrii and the pathogenic Bordetellae may not be orthologous.
Other protein secretion systems are differentially distributed among Bordetellae. In contrast to the mammalian pathogens, B. petrii and B. avium lack type I and III protein secretion systems, but encode a type II secretion system (Bpet2855-2866 and BAV0331-0343). Type IV secretion systems are either engaged in protein transport to the extracellular medium or into eukaryotic cells, or they are involved in DNA-transfer. There are three gene clusters in B. petrii, which are associated with GIs and appear to encode complete type IV secretion systems (Bpet2204-2215, Bpet3701-3719, Bpet4615-4626). None of these systems appears to be related to the pertussis toxin exporting type IV system of B. pertussis, being probably involved in conjugal DNA transfer.
The B. petrii genome carries three fimbrial gene clusters (Bpet0111-0114, Bpet4129-4132 and Bpet4464-4468), each encoding a major fimbrial subunit, a fimbrial assembly chaperone, an outer membrane usher protein and a fimbrial adhesin. The chaperone and usher proteins have significant sequence similarities with their counterparts in the pathogenic Bordetellae, with e-values ranging from 6e-23 to 3e-35 for the chaperones and e-values ranging from 3e-71 to 6e-103 for the usher proteins. In contrast, the homology of the major structural proteins and fimbrial adhesins with the proteins of the pathogenic Bordetellae is very low (e-values > 1e-5). Similarly to B. avium, B. petrii lacks the genes for the biosynthesis of siderophores. However, it specifies 12 TonB-dependent iron scavenging receptors, which may be important for iron acquisition in soil and plant-associated environments.
Most virulence factors are coordinately regulated in pathogenic Bordetellae by a single master regulator, the BvgAS two-component system [42, 43]. This system consists of two proteins, a response regulator (BvgA) and a multidomain histidine kinase (BvgS), consisting of a periplasmic solute-binding domain, and, on the cytoplasmic side, of a PAS, a transmitter, a receiver and an HPt domain. Although the B. petrii BvgA (Bpet 4471) is highly homologous to the BvgA proteins of the pathogenic Bordetellae (65% amino acid similarity), the structure of the B. petrii equivalents of BvgS is more complex. First of all, there are two histidine kinases, BvgS1 (Bpet4469) and BvgS2 (Bpet4472), with significant sequence similarities to BvgS. BvgS1 contains a PAS, a transmitter and a receiver domain, whereas BvgS2 contains a transmitter and a receiver domain. In contrast to BvgS, both B. petrii proteins are devoid of a large periplasmic domain. The HPt domain, which in pathogenic Bordetellae is part of the multidomain BvgS protein, in B. petrii is an independent protein (Bpet4470) (see Additional file 4. To see legends for Additional files 1, 2 and 4 please see Additional file 5 ). This suggests that during their evolution to pathogens, the virulence regulatory system has been streamlined by the omission of one histidine kinase and incorporation of the HPt domain into the multidomain histidine kinase BvgS.
Therefore, B. petrii is endowed with several putative virulence factors for plant and animal hosts, such as adhesins (e.g., Fha, fimbriae) and a master virulence regulator (Bvg), but lacks Bordetella toxins. This suggests that pathogenic Bordetellae might have acquired their toxin genes horizontally, while adapting to an obligate pathogenic lifestyle. Alternatively, it is possible that toxin genes have been lost from the B. petrii lineage.
The five sequenced Bordetella species, B. bronchiseptica, B. pertussis, B. parapertussis, B. avium and B. petrii, represent different states of environmental adaptation. These five organisms have followed different evolutionary paths. Evolution of the B. bronchiseptica-derived host restricted-pathogens B. pertussis and B. parapertussis was dominated by substantial gene decay and loss. This might also hold true for B. avium, which is characterized by a relatively small genome containing 68 pseudogenes. In contrast, the evolution of the environmental isolate B. petrii was dominated by horizontal acquisition of large genomic islands expanding its metabolic capacities. B. bronchiseptica, with a broad host range and the potential to survive in the environment, might represent an intermediate evolutionary state. If the Bordetellae derive from a common environmental ancestor, B. petrii appears to be more closely related to this ancestor than the pathogenic Bordetellae, since it is the only true environmental isolate within the genus, which is also found in association with host organisms. Moreover, B. petrii is the only facultative anaerobic Bordetella species . Since closely related Achromobacter species are also facultative anaerobes, the common ancestor of the Bordetellae probably had a facultative anaerobic energy metabolism. During adaptation to an obligate host association, the genes for several metabolic functions, e.g. those required for denitrification, may have been deleted from the genomes of pathogenic Bordetellae, leaving just a few remnants in the various species.
Whole genome shotgun sequencing
For the shotgun phase we produced 103,308 paired-end sequences derived from three pTZ18R genomic libraries (with insert sizes of 1 kb, 3 kb and 10 kb) yielding 11.72-fold coverage, using dye terminator chemistry on ABI377 and MegaBACE 1000 automated sequencers. As a scaffold we used 2,688 paired-end sequences pCC1BAC libraries with insert sizes of 17–30 kb and 40–70 kb (leading to a total coverage of 5.14-fold). Assembly of the paired-end sequences was performed by PHRAP (P. Green, unpublished data; http://www.phrap.org/). The complete genome sequence was finished by using the Staden package (GAP v4.8b1) . Another 5,154 sequencing reads were generated to close the remaining gaps and to polish the sequence.
Genome analysis and annotation
Curation and annotation of the genome was done using the genome annotation system GenDB . A combined gene prediction strategy was applied on the assembled sequences using GLIMMER and CRITICA . Putative ribosomal binding sites and transfer RNA genes were identified with RBSFINDER and tRNAscan-SE, respectively [47, 48]. In a first step, automatic annotation was computed based on various tools: similarity searches were performed against different databases including SWISS-PROT, TrEMBL, KEGG, Pfam, TIGRFAM and InterPro. Furthermore, SignalP, helix-turn-helix and TMHMM were applied. Additionally, each gene was functionally classified by assigning of a Cluster of Orthologous Groups (COG) number and its corresponding COG category and Gene Ontology numbers [49, 50]. Finally, the annotation of each predicted gene was manually inspected and adjusted if necessary.
For comparative analyses, the annotated genome sequences of the following bacteria were imported into the genome annotation system GenDB : Bordetella avium 197N (gb;AM167904), Bordetella pertussis Tohama I (gb;NC_002929), Bordetella parapertussis 12822 (gb;NC_002928), Bordetella bronchiseptica RB50 (gb;NC_002927). Similarity searches were conducted against the genomes on the nucleotide and amino acid sequence level by using BLASTN and BLASTP, respectively . Synteny plots were created with GenDB . For the determination of the core genome common to all sequenced Bordetellae all the CDS were compared to each other reciprocally using BLASTP with an e-value < 1e-20 as a cut-off.
Detection of regions with atypical GC content
Genomic regions with atypical GC content were identified using the 'sliding window' technique with a window size of 3,000 bp and a step size of 1,000 bp. For this purpose, the GC content was assumed to follow a Gaussian distribution and regions with at least 2.0 standard deviation differences from the mean were determined.
Use of aromatic compounds as sole carbon source by B. petrii
Growth experiments were carried out in a minimal medium containing 10% salt solution (2.5 g NaCl, 0.5 g KH2PO4, 0.2 g KCl, 0.1 g MgCl2 × H2O, 1.5 g Tris/HCl resuspended in 100 ml distilled water), 1.8 μM CaCl2, 36 μM FeSO4 × H2O, 2 mM (NH4)SO4. Aromatic carbon sources were added to final concentrations of 2–5 mM each.
Determination of TCT production
Tracheal Cytotoxin (TCT) was quantified as previously described .
The complete, annotated genome sequence has been submitted to EBI under the accession number AM902716.
This work was supported by the Pathogenomics Competence Network of the BMBF and by the priority research programme SFB479/A2 from the Deutsche Forschungsgemeinschaft.
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