- Research article
- Open Access
Dynamic transcriptome profiles of skeletal muscle tissue across 11 developmental stages for both Tongcheng and Yorkshire pigs
© Zhao et al.; licensee BioMed Central. 2015
Received: 27 January 2015
Accepted: 24 April 2015
Published: 12 May 2015
The growth and development of skeletal muscle directly impacts the quantity and quality of pork production. Chinese indigenous pig breeds and exotic species vary greatly in terms of muscle production and performance traits. We present transcriptome profiles of 110 skeletal muscle samples from Tongcheng (TC) and Yorkshire (YK) pigs at 11 developmental periods (30, 40, 55, 63, 70, 90, and 105 days of gestation, and 0, 1, 3, and 5 weeks of age) using digital gene expression on Solexa/Illumina’s Genome Analyzer platform to investigate the differences in prenatal and postnatal skeletal muscle between the two breeds.
Muscle morphological changes indicate the importance of primary fiber formation from 30 to 40 dpc (days post coitus), and secondary fiber formation from 55 to 70 dpc. We screened 4,331 differentially expressed genes in TC and 2,259 in YK (log2 ratio >1 and probability >0.7). Cluster analysis showed different gene expression patterns between TC and YK pigs. The transcripts were annotated in terms of Gene Ontology related to muscle development. We found that the genes CXCL10, EIF2B5, PSMA6, FBXO32, and LOC100622249 played vital roles in the muscle regulatory networks in the TC breed, whereas the genes SGCD, ENG, THBD, AQP4, and BTG2 played dominant roles in the YK breed. These genes showed breed-specific and development-dependent differential expression patterns. Furthermore, 984 genes were identified in myogenesis. A heat map showed that significantly enriched pathways (FDR <0.05) had stage-specific functional regulatory mechanisms. Finally, the differentially expressed genes from our sequencing results were confirmed by real-time quantitative polymerase chain reaction.
This study detected many functional genes and showed differences in the molecular mechanisms of skeletal muscle development between TC and YK pigs. TC pigs showed slower muscle growth and more complicated genetic regulation than YK pigs. Many differentially expressed genes showed breed-specific expression patterns. Our data provide a better understanding of skeletal muscle developmental differences and valuable information for improving pork quality.
The development of skeletal muscle affects meat production and growth rate in pigs. The genetic mechanisms controlling these traits are being uncovered [1,2]. In addition, the selection of meat quality based on molecular regulation is important for livestock breeding, and there have been many studies on skeletal muscle in different pig breeds [3-8]. The total number of fibers is constant in the postnatal stage, and postnatal muscle growth is through hypertrophy of myofibers, or conversion of myofiber types [9,10]. Primary and secondary fibers emerge at distinct embryonic stages during the skeletal muscle development of pigs . Primary myofiber formation occurs in the first wave of fiber generation, from about 35 days of gestation until around 60 days. Secondary myofibers appear at around 50–60 days based on the template of the primary myofiber surface. The third wave involves the process of transition between slow-oxidative and fast-glycolytic fiber types, which occurs from birth to 60 days of age [9,12,13]. Muscle maturation is completed in the early postnatal period . Therefore, we chose the critical skeletal muscle developmental stages across the prenatal to postnatal periods (30, 40, 55, 63, 70, 90, and 105 days of gestation, and 0, 1, 3, and 5 weeks of age) for studying the regulatory mechanisms and expanding the molecular genetics of muscle fiber development.
Many genes or factors participate in the process of muscle fiber formation, and muscle development is under complex genetic regulation. It is known that the muscle regulatory factor (MRF) and myocyte-specific enhancer binding factor 2 (Mef2) gene families play critical roles in regulating muscle fiber development. MRF genes are muscle-specific transcription factors, and induce myoblast proliferation or fusion differentiation during distinct stages of myogenesis . Mef2 regulates muscle-specific transcription during myogenesis and is activated during the development of the muscle [16,17]. However, knowledge of the functional genetics of myogenesis is currently insufficient and incomprehensive. Monitoring the transcriptional profiling from the prenatal to postnatal developmental stages in pigs will act as an animal model that will not only uncover the regulatory mechanisms behind factors affecting muscle development , but will also benefit the understanding of diseases affecting human muscle and muscular atrophy . With biotechnological advances in gene expression profiling, we have the ability to study large-scale differential regulation of genes genome wide, and to measure the transcriptional responses to certain complex biological conditions . Previous studies have presented expression analysis of myogenesis using microarrays [21,22], long SAGE , and differential display RT (reverse transcription)-polymerase chain reaction (PCR) . Digital gene expression technology is sensitive for the detection of low-abundant transcripts and small changes in gene expression , and has been applied extensively to compare the differences in transcription profiles of different tissues or breeds [15,26]. We present a panorama of transcriptome-wide longissimus dorsi muscle development using digital gene expression with Solexa/Illumina’s Genome Analyzer platform to study the variation between the dynamic transcriptome profiles of Tongcheng (TC) and Yorkshire (YK) pig breeds.
Previous studies have demonstrated differences in gene expression in pig longissimus dorsi muscle during fetal development, and some have shown breed-specific expression profiles [8,15,23,27-29]. Indeed, differences exist in muscle traits such as muscularity and muscle fiber type; muscle characteristics are different between pig breeds, and molecular biomarkers have been reported . However, the molecular genetic mechanisms of these breed-specific differences remain unclear, in terms of meat quality and visual phenotype. In this study, we selected the TC pig, a characteristic indigenous pig breed from the Hubei Province of central China with slow growth and prolific reproduction , and the YK pig, which is characterized by fast growth, low back fat, and a high lean meat percentage, to survey the differences in transcription. Previous studies have shown that indigenous swine breeds have slower growth rates and less lean meat percentages than exotic pigs [7,15,23]. Our study opens up the scope of knowledge of muscle-specific regulation and the microscopic molecular mechanisms. Our objective was to analyze the biological information regarding the transcriptional profiles of longissimus muscle between the two breeds and to further identify key gene regulatory networks and breed-specific pathways affecting skeletal muscle development in the pig.
Histological section and fiber size
We evaluated the morphological differences of skeletal muscle development between TC and YK pigs, from the histological appearance through the prenatal and postnatal stages. Histological cross-section (Additional file 1: Figure S1) showed that myoblasts developed quickly from 30 dpc (days post coitus), but there was no differentiation into primary fibers in either pig breed at 30 dpc, and the number and density of myoblasts in TC was more than that in YK. The myoblasts differentiated gradually, with primary fibers emerging from 30 to 40 dpc, and could be seen in the histological section to be fully formed at 40 dpc. Primary fibers continued to expand and enlarge until 63 dpc. Meanwhile, the primary fiber bundles were separated by the connective tissues. Secondary fibers appeared at 55 dpc in YK pigs and formed until 63 dpc, but they emerged later in TC pigs. Secondary fibers could be seen clearly around the primary fibers at 63 dpc, and the secondary fibers increased gradually until 90 dpc. The fiber bundles fused at around 90 dpc and appeared to form into the myotubes at 105 dpc; the primary and secondary fibers were not distinguishable at this period. The intramuscular fat texture showed differences in the histological section in the latter period between TC and YK pigs. These differences are associated with the different development of fat deposits in these two breeds. The muscle fibers grew rapidly, and finally the muscular tube formed and constituted the muscle mass. The phenotypes and sizes of the muscle fibers still varied based on the cross-sectional areas during the postnatal development stages (1, 3, and 5 weeks post natum (wpn) for both TC and YK pigs).
Solexa sequencing and tag mapping
After filtering adaptor tags, empty tags, low-quality tags, and one-copy tags from the raw data, 61,829,793 total clean tags were obtained in the TC breed, including 1,726,839 distinctly clean tags. 63,122,631 total clean tags and 1,684,960 distinctly clean tags were obtained in the YK pig (Additional file 2: Table S1). The total clean tags accounted for 93.6% and 94.3% of the raw tags in TC and YK, respectively. The number of unknown distinct tags was 32,884 and 33,141, on average, in each sample from TC and YK pigs. Moreover, the heterogeneity and redundancy of the mRNA were confirmed, and the results showed that the high copy number clean tags (with a copy number of more than 100) accounted for 76.5% of the total clean tags in TC and 77.5% in YK, while the low copy number clean tags (with a copy number of less than 2) accounted for less than 1% of the total clean tags in both breeds. Additional file 3: Figure S2 illustrates the distribution of total clean tags and distinct clean tags of TC and YK. The type of distinct tags were gradually stable, with the total number of sequence tags rising according to saturation analysis, and the percentage of genes identified was no longer increasing when the library size approached 3 million (Additional file 4: Figure S3). The number of tags mapping to genes was 283,853 and 283,869 in TC and YK, and the number of genes with unambiguous tag mapping was 200,320 and 199,991. The number of unambiguous tags mapping to genes accounted for 70.6% of all tags mapping to genes, and 58.9% of the total clean tags for the TC breed, in contrast to 70.4% and 60.0% for the YK breed (Additional file 2: Table S1).
Differential gene expression analysis and validation of Solexa sequencing data
Cluster analysis of DE genes
GO analysis of DE genes
Pathway enrichment analysis between TC and YK
Top 10 functional enrichment pathways in Tongcheng pigs
Cardiac muscle contraction
Protein digestion and absorption
MAPK signaling pathway
Citrate cycle (TCA cycle)
Arginine and proline metabolism
Top 10 functional enrichment pathways in Yorkshire pigs
Cardiac muscle contraction
Ribosome biogenesis in eukaryotes
Citrate cycle (TCA cycle)
Previous studies have investigated the differences in gene expression patterns or molecular genetic mechanisms of skeletal muscle development between characteristically different pigs [5,8,37,38]. Our studies will be helpful to explore and elucidate the control mechanisms for muscle development and growth on a genome-wide scale. Moreover, TC pigs, a typical local breed in central China, have a developmental period with three time points for skeletal muscle , which may limit studies on muscle development; comparative genomics could uncover the determining factors for the genetic basis of biological functions . The pig genomic sequence is very similar to the human genome, even though pigs and humans are divergent species . Transcriptional profiling in our study may provide a reference for studies on human muscle tissue development and dysfunction.
Muscle fiber development occurs in two waves, around 35 and 65 dpc, which involve the formation of primary and secondary fibers [11,28]. In our study, primary and secondary fibers formed at approximately 40 and 63 dpc (Additional file 1: Figure S1), and the muscle fiber diameter at birth was larger in the YK pig than the TC pig. Previous studies have also shown that the muscle fiber diameter of a Chinese indigenous pig (Langtang, LT) is smaller than an exotic pig (Landrace, LR), and that myogenesis starts earlier but progresses more slowly in LT pigs than in LR pigs . Likewise, Duroc and Pietrain pig breeds are also extremes, with myogenesis initiating earlier in Duroc pigs than in Pietrain pigs . The number of myoblasts in early TC embryos was more than that of early YK embryos, which may be the result of the proliferation and differentiation of myogenic precursor cells beginning earlier and developing more intensely in early TC embryos than in early YK embryos. It is known that the number of muscle fibers determines the capacity for postnatal muscle fiber growth and hypertrophy. Importantly, postnatal muscle fiber hypertrophy is inversely correlated with the total number of fibers in the muscle , which may be a reason for the slower growth rate and lower lean meat content for TC pigs than for conventional western pig breeds . In the histological cross-sections, muscle fiber development and differentiation were slower for TC pigs than for YK pigs. The intramuscular fat texture showed differences in the later period of muscle development between YK and TC, which may be the consequence of different expression profiles of fatty acid metabolism in the two extreme muscularity breeds .
Gene expression profiles can be investigated easily and extensively using RNA sequencing technology, with the results of these studies showing high levels of reproducibility in both technical and biological replicates [43,44]. To investigate the transcription profiles of TC and YK pigs reliably and comprehensively, we used five muscle samples at each stage, and evaluated the sequencing quality of each library of 110 samples (Additional file 4: Figure S3). As large and comprehensive bio-information resources were obtained, transcripts at low abundance and novel transcripts could also be detected. The distributions of total clean tags and distinct clean tags suggest that we obtained high-quality sequencing; and there were a high proportion of lower copy number tags (Additional file 3: Figure S2), which showed many genes expressed at a low level. We validated our results using real-time qPCR, which demonstrated that the quality of the Solexa sequencing is reliable and trustworthy. Moreover, qPCR measures a single specific gene, while Solexa sequencing involves massive parallel sequencing, and the abundance of the transcript can be affected by the test method and mRNA samples storage.
More DE genes were detected in the early period of muscle development (Figure 1), which reflects that the differences in the expression of muscle development genes are more intense in the period of primary fiber formation than in the period of secondary fiber formation . There were 2,462 DE genes in TC pigs and 864 DE genes in YK pigs that were involved in myoblast differentiation at this stage, which indicates that TC pigs at the early stage have a more complex regulatory mechanism of initiation. In addition, cluster analysis showed that there were differences in expression patterns between TC and YK pigs, and that the gene expression patterns at 1, 3, and 5 wpn were more similar in YK than TC pigs (Figure 3). 105 dpc and 0 wpn (natal day) were more similar in TC pigs than YK pigs; however, 30 dpc was an exception. The differences at the prenatal stage were greater than the postnatal differences in the two breeds overall. These results show that muscle morphological cross-sections clearly varied from 40 to 105 dpc (Additional file 1: Figure S1), and that the primary and secondary fibers were forming and changing during these stages, which is important for the growth and development of muscle fibers.
We further analyzed the gene expression patterns related to muscle fiber development (Figure 6). MyoG and MYF6 are myogenic differentiation factors  that participate in myoblast fusion and differentiation [27,49]. In our study, the expression level of MyoG was high at 70 and 90 dpc in TC pigs and 55 and 70 dpc in YK pigs, but obviously decreased after 90 dpc. MYF6 was at a low level at 30 dpc, and then began to increase at 40 dpc in TC and YK pigs, which indicates that it may be involved in differentiation before and after birth. The MEF2 and MRF families coordinate the regulation of muscle-specific genes and myogenic differentiation. Mef2d deletion in mice has little effect on skeletal muscle development, but deletion of Mef2c has been shown to cause defects in muscle formation . Additionally, Mef2c and Mef2d also play a role in muscle regeneration of satellite cells . Mef2c and Mef2d have similar expression patterns in TC and YK breeds, but Mef2c showed a higher expression level in our study. Myostatin (MSTN) is an inhibitor of skeletal muscle growth , and knockout mice show a dramatic increase in muscle mass . Our data show high expression of MSTN in two phases, 40–55 dpc and 70–90 dpc, to inhibit over-proliferation of myoblasts when the primary and secondary fibers are forming. MYOZ1 encodes a structural protein that constitutes the Z-lines in skeletal muscle. The expression of MYOZ1 increases gradually with age in both breeds, but was higher in early TC embryos, and lower in later TC embryos and piglets, than in YK. IGF2 is a critical regulatory factor for growth and differentiation of mammalian muscle and fetal development ; the sequencing results show high expression of IGF2 across 40–105 dpc, and lower expression after birth in TC and YK pigs. This suggests that IGF2 plays an important role during the key stages of primary and secondary fiber growth and development in the prenatal stage. Pathway analysis showed that ENO3 is involved in the glycolysis biological process, and participates in energy metabolism for muscle development . The ENO3 expression level was higher in the postnatal stage than the prenatal stage in both breeds, but was higher in later YK embryos and piglets than in the TC breed. This indicates that ENO3 is important for muscle development in the later period, and mainly provides energy by glycolytic metabolism.
We provide a new insight into transcriptional profiles of skeletal muscle development at 11 stages of TC and YK pigs using a genome-wide deep sequencing method. The critical stage for the formation of primary and secondary fibers is from 40 to 63 dpc for both Tongcheng and Yorkshire pigs. Muscle development in early TC embryos is more intense than in YK embryos, and is more intense in YK piglets than in TC piglets from the natal day to 1-week postnatum; this suggests that muscle fiber formation in TC pigs is initiated earlier than in YK pigs (30–40 dpc), while the muscle maturation processes are more complicated in early YK piglets (0–1 wpn). Many DE genes related to muscle development show phase-specific regulatory mechanism in myogenesis. Moreover, muscle contractile speeds and locomotion capacity are different in TC and YK pigs, which may be associated with more MyHC-I fibers in TC pigs and more MyHC-IIb fibers in YK pigs. The transition of energy metabolism between the oxidative phosphorylation and gluconeogenesis pathways affects the characteristics of the muscle fiber types. Furthermore, this study of skeletal muscle transcriptional profiles is helpful in understanding the differences between the genetic mechanisms in TC and YK pigs, and for future work exploring breed-specific changes in muscle development.
The experimental animal procedures were followed in accordance with the approved protocols of Hubei Province, PR China for the Biological Studies Animal Care and Use Committee.
Animals and muscle tissue preparation
Muscle tissue samples were obtained from 11 time points of skeletal muscle developmental stages for two breeds. Purebred TC and YK gilts (n = 40 per breed) were bred to purebred boars of their respective breed. Thirty-five gilts were slaughtered at each of seven time points for each breed (30, 40, 55, 63, 70, 90, and 105 dpc (days post coitus)) and the longissimus muscle tissue was rapidly dissected from each fetus (70 fetus samples). The remaining five gilts were allowed to farrow and longissimus muscle was collected from the piglets from each litter at 0, 1, 3, and 5 weeks of age (40 piglet samples). One hundred and ten muscle samples were taken and prepared. All samples were immediately snap frozen in liquid nitrogen and stored at −80°C until further use.
RNA quality assessment, cDNA library construction, and sequencing
Total RNA was extracted from the 110 frozen longissimus muscle tissues using TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer’s instructions. The RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) and met the experimental requirement of the Solexa sequencing platform. The quality of all the sample solutions had RNA integrity numbers (RIN) ≥ 7.0 and 28S/18S ≥ 1.0. RNA libraries were constructed and Solexa sequencing was performed by the Beijing Genomics Institute (BGI) on an Illumina Genome Analyzer. Sequencing tags were obtained using Illumina’s Digital Gene Expression Tag Profiling Kit according to the manufacturer’s instructions. These tags were aligned and identified based on two reference genomes, Ensembl Sus scrofa 10.2.68 and the UniGene database. Clean tags were obtained after filtering the adaptor tags, empty tags, low-quality tags, and one-copy tags from the total tags, and then they were aligned to the reference database and annotated.
Clean tags of each library were normalized by means of Tags per Million (TPM) to obtain normalized gene expression levels. DE genes were determined using the NOISeq-bio methods described by Tarazona et al. . The fold changes (log2 ratio) were estimated according to the normalized gene expression level in each sample. We used the absolute value of log2 ratio > 1 and probability > 0.7 as the threshold to judge significant differences in gene expression.
Similarities in gene expression patterns usually indicate that those genes have similar functions. The purpose of the clustering analysis was to screen the function of similar genes and cluster them together in the TC and YK pig breeds, thereby finding information on the function of the unknown genes, or unknown functions of known genes. We analyzed the systematic cluster of the two pig breed libraries using MEV software  for all DE genes in each breed.
GO is suitable for gene functional classification for all types of prokaryotes and eukaryotes , and this was applied to analyze the main functions of the DE genes. A hypergeometric distribution method was used for the GO significant enrichment analysis function of the DE genes. All DE genes were aligned and mapped using the GO database (http://geneontology.org/) for TC and YK pigs, and then the significant enrichment terms and gene list of each term were obtained for both breeds. The GO analysis can be helpful in determining the main biological function of classification for the DE genes, and for exploring the molecular functions and biological processes. The false discovery rate (FDR) was calculated to correct the P-value for the GOs of all the DE genes.
Assignment of DE genes to KEGG pathways
Pathway analysis helps to further understand the distinct functions of gene regulation and identify the significant pathways of the DE genes according to the KEGG database (Kyoto Encyclopedia of Genes and Genomes), which is a major public database on pathway enrichment analysis  (http://www.kegg.jp/kegg/pathway.html). The DE genes of TC and YK pigs were aligned to the KEGG database based on the entire genome background, which revealed any significant enrichment for DE genes in the pathway. A P-value and FDR was returned after pathway analysis for each breed. The threshold of significance was defined by FDR <0.05. Furthermore, a heat map of significant functional pathway enrichment was gained between the 10 comparison groups of each breed according to the FDR value using the Genesis software (http://genome.tugraz.at/genesisclient/genesisclient_description.shtml).
Five DE genes were chosen to verify the accuracy of the sequencing results, which was tested by real-time fluorescent qPCR on an ABI 7900 platform (Life Technologies, carlsbad, USA). qPCR was performed using SYBR Green I Real-Time PCR Master Mix (Toyobo, Osaka, Japan) according to the manufacturer’s instructions. All PCR reactions were carried out in triplicate. The samples used for real-time PCR assays were the same as those for the Solexa sequencing experiments. The RT-PCR reaction was carried out in a total reaction mixture of 20 μL as follows: 0.3 μL of F primer (10 mM), 0.3 μL of R primer (10 mM), 10 μL of SYBR Green Real time PCR Master Mix, 0.5 μL of cDNA, and 8.9 μL of double-distilled H2O. The RT-PCR was performed as follows: 94°C for 4 min, 34 cycles of 94°C for 30 s, 57°C for 30 s, and 72°C for 30 s, and a final extension at 72°C for 5 min. Expression levels of commonly used internal genes were inconsistent and showed variation across the 11 different developmental stages of skeletal muscle between the TC and YK libraries. Therefore, the relative expression level of mRNA was calculated using RAD23B as an endogenous reference gene using the 2-ΔΔCt method. The Spearman’s correlation coefficient was further calculated for each gene using the normalized data to quantify the consistency between the Solexa sequencing and RT-qPCR experiments. Additional file 13: Table S9 lists the gene-specific primers for gene expression.
Histology and histochemistry of 22 samples from muscle fibers
We applied the hematoxylin and eosin method  to obtain the histology and histochemistry of the 11 different developmental stages of skeletal muscle fibers between TC and YK pigs. Each muscle tissue sample was processed routinely for paraffin embedding, and sections were cut and stained with hematoxylin and eosin for the muscle tissue morphogenetic contrast analysis. Micrographs were taken with the BX53 (Olympus Corporation, Tokyo, Japan) digital camera system. The muscle tissue image was magnified 400 times.
Supporting data information
The transcriptome raw data of Tongcheng and Yorkshire pigs were submitted to the Sequence Read Archive (SRA) of the National Center for Biotechnology Information (accession number: SRA234513).
This search project was supported financially by the National Porcine Industry Technology System (CARS-36) and the Engineering Technology Research for Animal (2011FU125Z22). We sincerely appreciate the experimental contributors who made efforts to complete this study; we also thank the Animal Husbandry Bureau of Tongcheng County for assistance with this study.
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