Genome-wide transcriptomic analysis of a superior biomass-degrading strain of A. fumigatus revealed active lignocellulose-degrading genes
© Miao et al. 2015
Received: 19 March 2014
Accepted: 28 May 2015
Published: 16 June 2015
Various saprotrophic microorganisms, especially filamentous fungi, can efficiently degrade lignocellulose that is one of the most abundant natural materials on earth. It consists of complex carbohydrates and aromatic polymers found in the plant cell wall and thus in plant debris. Aspergillus fumigatus Z5 was isolated from compost heaps and showed highly efficient plant biomass-degradation capability.
The 29-million base-pair genome of Z5 was sequenced and 9540 protein-coding genes were predicted and annotated. Genome analysis revealed an impressive array of genes encoding cellulases, hemicellulases and pectinases involved in lignocellulosic biomass degradation. Transcriptional responses of A. fumigatus Z5 induced by sucrose, oat spelt xylan, Avicel PH-101 and rice straw were compared. There were 444, 1711 and 1386 significantly differently expressed genes in xylan, cellulose and rice straw, respectively, when compared to sucrose as a control condition.
Combined analysis of the genomic and transcriptomic data provides a comprehensive understanding of the responding mechanisms to the most abundant natural polysaccharides in A. fumigatus. This study provides a basis for further analysis of genes shown to be highly induced in the presence of polysaccharide substrates and also the information which could prove useful for biomass degradation and heterologous protein expression.
Plant biomass is the most abundant natural material on earth and the only foreseeable sustainable source of fuels and materials available to humanity [1, 2]. It mainly consists of cellulose, hemicellulose, lignin, pectin and other polymers in ratios that vary between plant species . Cellulose, the main polymeric component of plant biomass , pt?>consists of D-glucoside residues linked by beta-1,4-glycosidic bonds to form linear polymeric chains of over 10000 glucose residues. Cellulose usually contains regions that are highly crystalline in nature . The hydrolysis of cellulose and the degradation of the other polysaccharides in plant biomass require many types of enzymes (e.g., endoglucanase, β-glucosidase, cellobiohydrolase, endo-β-1,4-xylanase, β-xylosidase and numerous other associated enzymes) that work in concert . Even when enzymes work synergistically, degradation of recalcitrant lignocellulose is more difficult than degradation of many other natural materials, such as starch .
The key step for the utilization of plant biomass (such as agricultural crop residues, grasses, wood and municipal solid waste) is to degrade it into sugars or oligosaccharides by means of various hydrolytic enzymes. The central technological impediment is the general absence of low-cost technology for overcoming the recalcitrance of lignocellulosic biomass, as the costs of cellulases and hemicellulases contribute substantially to the price [7, 8]. Studies aimed at understanding and increasing the efficiency and productivity of plant biomass-degrading enzymes are at the forefront of this research. By combining enzyme engineering methods (such as mutagenesis, DNA shuffling and protein recombination) with effective screening protocols , fold-increased activity and thermostability of enzymes have been achieved [10–12]. However, protein engineering strategies are not always effective and may only make relatively small changes to existing enzymes. Consequently, screening of high-efficiency strains and gene resources is critical for improving biomass utilization .
Filamentous fungi are widely used for their capacity to produce extracellular proteins in large quantities [13, 14]. As a result of their saprotrophic lifestyle, filamentous fungi can secret a wide range of enzymes into their habitats, enabling them to metabolize a variety of plant polysaccharides. One filamentous fungus, Neurospora crassa, has long served as a model fungus [15, 16] and the molecular techniques developed for N. crassa studies have also been widely applied to other filamentous fungi . Meanwhile, Aspergillus genus species (including A. niger, A. nidulans and A. oryzae) have long been used in the enzymatic industry [18, 19]. Another industrial fungus, Trichoderma reesei, was reported to have the ability to produce 100 g of extracellular cellulases per liter . Whole genomes of all of these fungi have been sequenced, which has greatly facilitated the understanding of their highly efficient biomass-degradation mechanisms [21, 22], the design of enzyme cocktails [5, 23], and the engineering of strains for higher protein expression.
As we know, A. fumigatus is as versatile in nature as other model fungi. However, there are few detailed studies of its ability to degrade plant biomass, even though some strains of this species are recognized as potent producers of lignocellulolytic and hemicellulolytic enzymes [24, 25]. In our previous study A. fumigatus Z5 isolated from compost heaps of plant straw, has been shown to produce highly thermostable lignocellulosic enzyme activities . Many of the glycoside hydrolases are closely related and have redundant (or partially overlapping) functions, which may facilitate the survival of A. fumigatus Z5 under different environmental conditions (e.g., variations in pH, temperature, ionic strength) or may be necessary to effectively depolymerize complex carbohydrate polymers. In order to further understand the biomass-degradation mechanism used by this fungus and to facilitate the development of its applications, the genome sequence of A. fumigatus Z5 was determined and the transcriptomic profiles induced by sucrose, xylan, cellulose and rice straw were compared. Combined analysis of the genomic and transcriptomic data provides a comprehensive understanding of the responding mechanisms to the most abundant natural polysaccharides in A. fumigatus, as well as a roadmap for biomass utilization and the industrial application of gene resources or as a host for protein expression.
General genomic features and comparison with several industrial strains
Genome features of A. fumigatus Z5
G + C content (%)
Protein coding genes
Mean gene length (bp)
Percent genes with introns
Genes of unknown function
Mean number per gene
Mean length (bp)
G + C content (%)
Mean number per gene
Mean length (bp)
G + C content (%)
Mean length (bp)
G + C content (%)
Specific proteins in six filamentous fungi
A. fumigatus Z5
Carbohydrate-active enzymes in A. fumigatus Z5
Carbohydrate-active enzymes (CAZymes) are categorized into different classes (glycoside hydrolases, glycosyltransferases, polysaccharide lyases, carbohydrate esterases, auxiliary activities and carbohydrate-binding modules) in the CAZy database (http://www.cazy.org) . CAZymes cleave, build and rearrange oligo- and polysaccharides. These functions play important roles in fungi such as A. fumigatus and are vital for optimizing biomass degradation by these species . Given the importance of this protein family for biomass utilization, a detailed examination of the CAZome of A. fumigatus Z5 was performed.
CAZyme gene numbers in A. fumigatus and N. fischeri
A. fumigatus Z5
A. fumigatus AF293
A. fumigatus var. RP-2014
A. fumigatus A1163
N. fischeri NRRL 181
Protein secretion of A. fumigatus Z5
In contrast to other microorganisms, filamentous fungi can secret large amounts of proteins into the external environment. Strain Z5 is also an extraordinarily efficient producer of extracellular enzymes [25, 26], which makes the analysis of the protein secretion system particular interesting. Here, we compared some components of secretory pathway among strain Z5 and 5 other industrially relevant filamentous fungi extensively used for protein secretion (A. niger, A. nidulans, A. oryzae, N. crassa and T. reesei). Translocation from the cytoplasm to the endoplasmic reticulum (ER) occurs in these fungi through established signal recognition particle (SRP)-dependent and SRP-independent pathways , which are very important steps for heterologous gene expression. Orthologs of most S. cerevisiae proteins in these two pathways were found in strain Z5 and 5 other fungi except for a yeast signal recognition and docking protein, Srp21p . All these filamentous fungi have a gene encoding protein disulphide isomerase (PDI) (ortholog of yeast Pdi1p) and 3 other PDI-related genes. The chaperone BiP, a member of the heat shock 70 protein family, is more conserved (similarity > 90 %) than other components of secretory pathways in these fungi, reflecting the central role of BiP in translocation into the ER, protein folding and regulation response [37, 38]. Multiple enzymes are required for N-glycosylation of glycoproteins, which involves binding of a preformed oligosaccharide (Glc3Man9GlcNAc2) to asparagine side chains of an immature protein. All six fungi have at least one copy of the key enzymes for this process (glucosidase I and II, calnexin, calreticulin and UDP-glucose:glycoprotein glucosyltransferase). which indicates that they may have a common folding and quality control system for glycoproteins. The unfolded protein response (UPR) signaling pathway in these fungi differs somewhat from that of yeast. In mammalian cells, the PERK (double-stranded RNA-activated protein kinase-like endoplasmic reticulum kinase) machinery can attenuate protein translation by phosphorylating the alpha subunit of eukaryotic translation initiation factor 2 (eIF2α) in the early phase of ER stress . PERK is then inhibited by binding to the HSP40 cochaperone p58, the expression of which is induced by IRE1/XBP1 of the UPR pathway. The ortholog of p58 was found in the genomes of these fungi but not in yeast, and the ortholog of PERK was not found or had low similarity. Homologs of most known ERAD components were found in all these filamentous fungi, despite an apparent lack of orthologs and little sequence similarity to yeast ERAD components, as reported for the A. niger genome . Some counterparts of the mammalian GTPase proteins (Rab2, Rab4, Arf6 and Arf10), which are involved in membrane fusion and vesicle budding in diverse cellular locations, were found in all these fungi, whereas they were absent in yeast .
Transcriptional responses of A. fumigatus Z5 to different carbon sources
To validate the transcriptomic results, all the predicted cellulase genes were investigated by qRT-PCR under the induction of cellulose, which confirmed the transcriptomic results (Fig. 6b). Additionally, extracellular protein contents and total xylanase and cellulase activities of the 4 treatments (sucrose, cellulose, xylan and rice straw) provide further evidences to the transcriptomic data (Fig. 6c).
The MFS includes 82 protein families, 6 of which are known to transport sugars (the sugar porter (SP) family, the oligosaccharide:H+ symporter (OHS) family, the fucose:H+ symporter (FHS) family, the sialate:H+ symporter (SHS) family, the polyol permease (PP) family and the sugar efflux transporter (SET) family), in addition, the glycoside-pentoside-hexuronide (GPH):cation symporter family is also responsible for sugars transportation . Totally, strain Z5 has 88 sugar transporter genes distributed among SP, FHS, SHS and GPH families (SP family includes 79 genes), 37 genes were differently expressed in polysaccharides-induced samples when compared to sucrose control, the 8 genes which expression changed the most were predicted to transport glucose, xylose, lactose and maltose. Three (Y699_03783, Y699_04557 and Y699_04571) and six sugar transporter genes (Y699_01300, Y699_02331, Y699_03701, Y699_07512, Y699_09366 and Y699_09270) were only induced by xylan and cellulose, respectively. Gene Y699_09366 was up-regulated about 200 folds by both cellulose and rice straw, which is the homolog of a lactose permease gene (Gene ID: 3450) essential for cellulase induction in T. reesei v2.0 . All the homologs of the 37 genes could be detected in other 5 filamentous fungi (A. niger, A. nidulans, A. oryzae, N. crassa and T. reesei) even though N. crassa only has 44 sugar transporter genes, which indicated that filamentous fungi shared common mechanisms of sugar transportation.
GO enrichment analysis
Analysis of expression patterns in different treatments indicated that 444, 1386 and 1711 genes were up-regulated or down-regulated in the XGEP, RGEP and CGEP, respectively, in comparison to the SGEP (Fig. 8d). A GO term enrichment analysis of the three polysaccharide-induced gene expression patterns compared to the SGEP was performed with the topGO R package (Additional file 12) . XGEP vs. SGEP analysis showed enrichment of transmembrane transport (P = 1.1e-05), the D-xylose metabolic process (P = 1.3e-04), the xylan catabolic process (P = 7.5e-04) and the polysaccharide binding category (P = 9.3e-04). Comparison of RGEP with SGEP indicated enrichment of transmembrane transport (P = 1.4e-06), the cellulose catabolic process (P = 6.0e-05), the xylan catabolic process (P = 8.4e-06), the pectin catabolic process (P = 5.5e-05) and the polysaccharide binding category (P = 5.8e-12). The comparison of CGEP with SGEP revealed enrichment of carbohydrate transport (P = 7.5e-04), the cellulose catabolic process (P = 7.6e-05), the hemicellulose metabolic process (P = 7.5e-04), the polysaccharide binding category (P = 6.2e-10) and ribosome biogenesis (P = 1.4e-18). Together, these results show that A. fumigatus Z5 could adjust its gene expression and metabolic pathways by using different substrates as a carbon source. When xylan was supplied, Z5 had a higher expression level of xylanase genes and carbohydrate transporter genes, which could degrade xylan into xylobiose or xylose and then transport them into the cell. Xylobiose and/or xylose could be utilized to produce energy or synthesize other materials in the D-xylose metabolic process. Degradation enzymes, transporters and polysaccharide binding activity were also needed during the degradation of cellulose and rice straw. Intriguingly, CGEP showed enrichment of ribosome biogenesis, indicating that A. fumigatus Z5 needs more ribosomes for protein synthesis. The protein products might be secreted into the external environment and contribute to the difficult process of crystalline cellulose degradation.
Analysis of the transcriptome profiles across all scaffolds (Fig. 5 and Additional file 9) indicated that the expression levels of most genes were stable, while significant changes occurred in and around CAZyme-rich regions. A total of 468 genes were found in the 35 fragments; they ranged from 8 kb to 112 kb in length, and all were up-regulated or down-regulated in at least 1 of the 3 transcriptomes (Fig. 4 and Additional file 13). Among these fragments, eight segments corresponding to eight different CAZyme gene clusters were found, and five of them were up-regulated in all three transcriptomes compared to SGEP, while the others were down-regulated. These results suggested that the genes in and around the eight CAZyme gene clusters might be closely related to utilization of different carbon sources. Additionally, 11 of the 30 secondary metabolite biosynthetic gene clusters (Additional file 14) in the genome were located or partly located in the co-regulated fragments. Of those 11, five were up-regulated, and the remainders were down-regulated. Therefore, the secondary metabolism pathways might be stimulated in A. fumigatus Z5 when the complex organic substances exist in the environment, enabling the strain Z5 to fend off competition for nutrients and survive in complex environmental conditions.
Filamentous fungi efficiently degrade plant biomass and secret substantial amounts of extracellular enzymes into their substrates. Several model filamentous fungi, including N. crassa, T. reesei, A. niger, A. oryzae and A. nidulans, are widely used in industry and have been the subjects of numerous studies [16, 31, 36, 40, 48]. Unlike the three model species in the Aspergillus genus that have a long history of safe use for industrial enzyme production, A. fumigatus strains are opportunistic pathogens that may cause invasive aspergillosis in immunocompromised patients [29, 49], although strains in this species are also effective at biomass degradation. A. fumigatus Z5 was isolated from crop compost heaps , suggesting that the strain is widely distributed in the environment and contributes to the degradation of plant biomass. There have been some studies related to polysaccharide degradation in Aspergillus fumigatus [24, 26, 50, 51]. This study on strain Z5 showed that it has an abundant distribution of CAZyme genes in genome and can produce large amount of extracellular enzymes, which make it possible to be a producer of enzymes to degrade plant biomass. Also, as a fast growing filamentous fungus at a high temperature of 50 °C, strain Z5 could easily be a host for heterologous protein expression by the mature molecular technologies in A. fumigatus. Even though, strain Z5 was not as good as some well-studied fungi, such as A. niger MGG029 with many genomic modifications , but it shared a similar potential and results of the present study enable valuable resources for further explorations. The safety of this strain should be considered by knocking out some virulence-related genes, such as the genes for gliotoxin, fumagillin, aflatoxin and ribotoxin .
In general, filamentous fungi cannot absorb polysaccharides directly into their cells to use as sources of carbon and energy. Instead, they secrete many types of enzymes to depolymerize polysaccharides into monomeric carbohydrates that are efficiently taken up and metabolized. In eukaryotic cells, protein secretion involves ER-associated translation, folding and glycosylation. Proteins are then moved into the Golgi apparatus or other compartments via vesicles to form the mature proteins. Finally, within secretory vesicles, the mature proteins are transported to the cell membrane and secreted into the environment . Guillemette et al.  reported transcriptional responses of A. niger when exposed to secretion stress (treatment with tunicamycin, treatment with DTT or forced secretion of a heterologous protein). In total, 11 genes in the secretory pathway were induced by the three ER-stress conditions. However, among the homologous counterparts of these 11 genes in A. fumigatus Z5, seven were down-regulated in all three treatments (xylan, cellulose and rice straw) when compared to the sucrose as a control condition (Additional file 10). The same trend was observed among the important chaperones and foldases (bipA, pdiA and clxA), which are involved in protein folding in the ER, with the exception of a 1.3-fold increase in bipA observed on cellulose. Unfolded protein response (UPR) and ER-associated degradation (ERAD) were also not induced by the three polysaccharides. These results indicate that there was no ER stress in these conditions compared to sucrose, even though more extracellular proteins were secreted . Two genes involved in glycosylation, the α-1, 2-mannosidase gene (Y699_04911) and the α-glucosidase gene (Y699_09020), exhibited 1.5–22.3-fold and 3–76.4-fold increases, respectively, in the three treatments compared to sucrose control. It has been suggested that plant biomass-degrading enzymes are highly glycosylated . Our study indicates that when polysaccharides are the sole substrate for growth, high levels of extracellular enzymes do not cause ER stress. Indeed, it suggested that if the balance between growth and protein secretion of fungi is managed well, the secretory pathway can be fully explored, and fold-increased production of extracellular proteins can be achieved.
XlnR is a xylanolytic transcriptional activator specific to fungi  that effects a wide range of target genes, e.g., genes encoding xylan-degrading enzymes or the enzymes in the D-xylose metabolic pathway [57, 58] and even genes encoding cellobiohydrolases and endocellulases . In this study, the XlnR gene was significantly up-regulated by cellulose and rice straw but not by xylan. Andersen et al.  reported that in the fermentations of glucose and xylose, 22 conserved genes were differentially transcribed (21 up-regulated and one down-regulated) in three Aspergillus species (A. niger, A. oryzae and A. nidulans) when xylose, rather than glucose, was the substrate. Enzymes involved in D-xylose metabolism were among the 22 genes. In the present study, homologs of the 21 up-regulated genes were mostly transcribed at high levels in the three treatments compared with sucrose control (Additional file 15). However, the results indicated that D-xylose metabolism was not activated by XlnR because its transcriptional level was similar in xylan and sucrose, even though the D-xylose metabolic pathway was significantly induced in xylan. The down-regulated gene identified by Andersen et al. encoded a MFS monosaccharide transporter, which was reported to have a higher affinity for glucose than xylose in their results. The expression level of the homolog gene decreased considerably in the three polysaccharides treatments of this study, suggesting that either the cellulose and rice straw treatments did not have enough glucose to induce the transporter gene or cellulose was transported into cells mainly as a form of cellobiose rather than glucose . Another interesting finding in the 22 genes is that a sugar transporter (Y699_05888) had a extremely high expression level in xylan and rice straw (81.9-folds and 64.6-folds, respectively) but only a 4.7-folds enhanced in cellulose, which revealed that this sugar transporter should be a specific transporter of the hydrolyzing products from xylan in all four Aspergillus species (qRT-PCR validated that gene Y699_05888 was up-regulated 2.8 ± 0.08 and 27.2 ± 0.52 folds by cellulose and xylan, respectively).
Transcriptomic data showed that genes encoding hydrolytic enzymes involved in plant cell wall degradation were repressed during growth on sucrose. Carbon catabolite repression (CCR) is an important mechanism to repress the production of polysaccharide-degrading enzymes during growth on easily metabolized carbon sources. In most fungal species, CCR is mediated by a zinc-finger transcription factor CreA [62–64], which is a repressor for genes encoding polysaccharide-degrading enzymes. In this study, we expected to have low CreA expression levels in the 3 polysaccharides treatments when compared to sucrose treatment. However, the creA gene was transcribed at high levels (RPM = 4714.2 in rice straw and RPM = 3366.3 in cellulose), with 7.7- and 5.5-fold increases in rice straw and cellulose compared to sucrose, respectively. Reverse transcription quantitative PCR (RT-qPCR) experiments under the same conditions as the transcriptomic study confirmed these observations (data not shown). These results suggest that CreA has functions beyond those of a transcription repressor and merits further research.
Our previous research identified many plant biomass-degrading enzymes secreted by Z5 in the presence of different carbon sources (i.e., glucose, cellulose and rice straw) . The expression of those enzymes in the present study is consistent with the findings of the previous study. Additionally, xylanase genes were induced more by cellulose and rice straw than by xylan; xylan was not the best inducer of its own hydrolytic enzymes. We observed that cultivation broth containing xylan became transparent, while culture filtrates with avicel or rice straw still had unused substrates after 16 h of incubation, indicating that pure xylan was depolymerized faster than avicel and rice straw; our previous work showed that Z5 has very strong xylanase activities . We conclude that the simple carbon source, xylose, which is released by xylan degradation, inhibited the persistent high expression of many xylanases. This may also account for the smaller transcriptional differences between the xylan treatment and sucrose control than those observed when either cellulose or rice straw treatments are compared to the control.
This study performed transcriptional analysis on an A. fumigatus strain grown on xylan, cellulose and rice straw. It comprehensively described natural polysaccharide saccharification by A. fumigatus hydrolases and the regulation of gene expression that occurs during this process. Having identified enzymes actively involved in biomass degradation, we foresee the potential to increase enzyme activities in industrial contexts. This would first require the heterologous expression of the enzymes at high levels and optimization of their incorporation into enzymatic cocktails. The genome and transcriptome data of this study are also valuable for future studies of aspergillosis.
Genome assembly, scaffolding, and gene prediction
The genome of A. fumigatus strain Z5 was shotgun sequenced using a Roche 454 GS FLX system at the Chinese National Human Genome Center (Zhangjiang Hi-tech Park, Shanghai, China). It produced 837 Mb of sequence data (29.9 × coverage) with an average read length of 358 bp. Using the Newbler software (v2.3) within the Roche 454 suite package , these reads were assembled into 596 contigs with a total size of 29.4 Mb. For sequence scaffolding, Solexa Mate Pair reads (insert size 3 ~ 5 kb) were used to establish the genome scaffolds. A total of 38,248,825 mate-pair reads contributed to improving sequence quality and constructing scaffolds. A simple greedy algorithm was used to optimize the order of the contigs and provided a feasible heuristic solution for scaffolds construction . By mapping the reads to contigs, 557 contigs were then linked, generating 24 genome scaffolds with their length not smaller than 1 kb. For gene prediction, Genemark (v2.3 a, http://topaz.gatech.edu/GeneMark/) and Augustus (v2.5, http://bioinf.uni-greifswald.de/augustus/) were used to predict genes for the Z5 genome, and the genome of A. fumigatus AF293 was used as a reference. The predicted genes were annotated using public databases (NCBI nr, Uniprot and KEGG) with BLAST, and the predicted genes with the best supporting annotation results were selected as the gene models. As a result, a total of 9540 gene models were described in the genome. Transfer RNAs (tRNAs) were predicted with tRNAscan-SE  (http://lowelab.ucsc.edu/tRNAscan-SE/). Secreted proteins were identified by SignaIP 4.0 analysis  (http://www.cbs.dtu.dk/services/SignalP/).
Strains and culture conditions
Strain Z5 was grown on potato dextrose agar (PDA) plates for conidia production. Conidia were harvested by washing the plate with 10 ml sterile ddH2O. After removing the mycelia, the conidia were resuspended, and the concentration was adjusted to 1 × 107 conidia/ml. Basic culture medium (BCM) was composed of the following: 0.25 g/L yeast extract, 1 g/L MnSO4 · H2O, 0.5 g/L urea, 0.5 g/L (NH4)2SO4, 0.5 g/L MgSO4 · 7H2O, 7.5 mg/L FeSO4 · 7H2O, 2.5 mg/L MnSO4 · H2O, 3.6 mg/L ZnSO4 · 7H2O, 3.7 mg/L CoCl2 · 6H2O, 0.5 g/L CaCl2 and a predetermined concentration of carbon source according to our experimental conditions . 1 × 107 conidia/L were inoculated into liquid BCM with sucrose as the carbon source (BCM-S) and cultured at 45 °C for 24 h. For cultivation in a medium containing xylan from oat spelts (Sigma), Avicel PH-101 (Sigma-Aldrich) or rice straw as a carbon source, the mycelia grown in the BCM-S were exhaustively washed with sterile distilled water and then transferred into yeast-free BCM and containing 1 % oat spelts xylan, 1 % Avicel PH-101 (a source of cellulose), or 1 % rice straw as a carbon source for 16 h. Mycelia were harvested by filtration through one layer of gauze, washed thoroughly with sterile water and quickly frozen in liquid nitrogen for further RNA extraction. The reason for sucrose used to be control is that sucrose is a favored carbon source to polysaccharides, and it will not induce any lignocellulolytic genes in filamentous fungi. For BCM with rice straw, the rice straw obtained from the farmland was exhaustively washed with deionized water for several days until reducing sugars were not detected by dinitrosalicylic acid (DNS). The rice straw was then dried completely at 40 °C, made into rice straw powder using a grinder and stored at room temperature.
Extracellular proteins were precipitated by 80 % ammonium sulfate and redissolved in sterile distilled water. The protein concentration was determined with a Micro BCA protein assay kit (Beyotime, China) using bovine serum albumin as a standard. Cellulase and xylanase activities were measured by 3, 5-dinitrosalicylic acid (DNS) method according to Liu et al. . One unit of enzyme activity was defined as the amount of enzyme required to release 1 μmol of reducing sugars from the substrate in 1 min.
RNA extraction and qRT-PCR experiments
The frozen mycelia were disrupted by grinding in liquid nitrogen, and total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen) combined with the RNase-Free DNase set (Qiagen). The total RNA (approximately 2 μg) extracted from each treatment was fractionated on a 1.2 % agarose gel, stained with ethidium bromide and visualized with UV light to check RNA integrity. The total RNA samples were then quantified using a NanoDrop 2000 spectrophotometer (Thermo, USA). For qRT-PCR analysis, cDNA were synthesized from the total RNA in different induction time using a PrimeScript™ RT-PCR Kit (TAKARA, DaLian, China) according to the manufacturer’s instructions. All quantitative PCR runs were performed in triplicate on a 7500 Fast Real-Time PCR System (Applied Biosystems) using SYBR® Premix Ex Taq™ Kit (Tli RNaseH Plus) (TAKARA, DaLian, China). An actin-encoding gene and a histone-encoding gene (GenBank Accession No: Y699_04988 and Y699_07225, respectively) were chosen together to be reference genes. The primer sequences are given in Additional file 16.
Transcriptome sequencing and gene mapping
Messenger RNA was purified from the total RNA with a Micropoly(A)PuristTM mRNA purification kit (Ambion, America). Double-stranded cDNA was synthesized according to Ng’s full-length cDNA synthesis protocol  with some modifications. GsuI-oligo dT was used as the primer for first-strand cDNA synthesis from 10 μg of mRNA, using 1,000 units of Superscript II reverse transcriptase (Invitrogen, America). The diol group of the CAP structure of mRNA was then oxidized by NaIO4 (Sigma, America) and linked to biotin hydrazide. Dynal M280 streptavidin Dynabeads (Invitrogen, America) were used to select biotinylated RNA/cDNA. The first-strand cDNA was then released by alkaline hydrolysis. Next, adaptors were ligated to the 5’-end of the first-strand cDNA by DNA ligase (TaKaRa, Dalian, China), and the second-strand cDNA was synthesized through primer extension using Ex Taq polymerase (TaKaRa, Dalian, China). Last, the polyA tail and 5’ adaptors were removed by GsuI digestion. The synthesized cDNA was fractioned ultrasonically into 300-800 bp fragments and purified using Ampure beads (Agencourt, America). The prepared cDNAs were transformed into libraries with the TruSeq DNA Sample Prep Kit Set A (Illumina, America), clonally amplified using the TruSeq PE Cluster Kit (Illumina, America) and sequenced with Illumina equipment according to the manufacturer's instructions. Clean reads (those in which at least half of the bases, excluding N, had a quality score above 5) were retained and mapped onto the genome or annotated genes of strain Z5. Reads were collected from the different samples for each gene, and read counts were transformed into RPM values (Reads Per Kilo bases per Million reads) . Using the MA-plot-based method with Random Sampling model in the DEGseq program package , the differences in expression levels between samples were calculated for each individual gene. Differences were determined to be significant if FDR scores were ≤ 0.001.
Gene expression analysis
The genes with significantly different transcriptional levels in all 4 transcriptomes need to have RPM (Reads Per Kilo bases per Million reads) scores above 10 in all transcriptomes as well as the coefficient of variation of the RPM values in all transcriptomes of not less than 0.6. The results were organized and visualized using Pearson correlations and hierarchical clustering with the average linkage clustering method to view the whole data set. K-means cluster analysis was used to group the genes into 10 clusters with the TIGR multiexperiment viewer (MeV, http://www.tigr.org/software). For the purposes of GO terms enrichment analysis, genes that differed significantly between treatment (XGEP, CGEP or RGEP) and control (SGEP) were those that had RPM ≥ 10, q-value ≤ 0.0001 and |log2 of the ratio of the RPM values| ≥ 2. GO terms annotation was accomplished by Blast2GO software (http://www.blast2go.com). Genes that differed significantly between treatment and control were analyzed and visualized to see the results of GO term enrichment using the classic algorithm and Fisher’s exact test in the Bioconductor topGO package (http://www.bioconductor.org/packages/2.12/bioc/html/topGO.html). Detrended correspondence analysis (DCA) was conducted using the vegan package of R . The transcriptome sequencing data and screening results are provided in Additional file 17.
Whole genome protein families were classified by InterproScan analysis (http://www.ebi.ac.uk/interpro/). The number of genes for each COG category was determined using a BlastP search against the COG data set. The highest scoring hit to COG category for each protein was retained. Using the same way, the sugar transporter family proteins were found out by blasting to Transporter Classification Database (TCDB, http://www.tcdb.org/). CAZyme proteins were classified with the CAZYmes Analysis Toolkit  (CAT, http://mothra.ornl.gov/cgi-bin/cat.cgi). Annotation based on sequence similarity and annotation based on association rules between CAZy families and pfam domains against the CAZy database (http://www.cazy.org/) were integrated for CAZyme protein predictions.
Gene cluster identification
The gene families were collected by visual inspection using CIRCOS software  (http://www.circos.ca). For CAZyme gene clusters, a cluster is defined as a region containing a statistically higher proportion of a particular gene family, and the cluster must begin and end with a gene from the family studied. We then calculated the probability of these CAZyme gene clusters based on a hypergeometric distribution, and all 44 clusters had a P value < 0.01. To analyze fungal clusters of secondary metabolism, the genome annotation data were coordinated and analyzed with the program SMURF (http://www.jcvi.org/smurf/index.php). The nucleotide fragments in which genes were nearly co-regulated (up- or down-) were also collected by visual inspection and compared to the clusters of secondary metabolism by Blastp.
This Whole Genome Shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession AZZA00000000. The version described in this paper is version AZZA01000000. The transcriptomes data discussed in this publication have been deposited in NCBI’s Gene Expression Omnibus  and are accessible through GEO Series accession number GSE55086 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE55086).
This research was financially supported by the Chinese Ministry of Science and Technology (2013AA102802) and the Chinese Ministry of Agriculture (2011-G27). DL was supported by the National Natural Science Foundation of China for Youth (31201685), and RZ and QS were also supported by the 111 Project (B12009) and the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions respectively. The authors thank Irina Druzhinina and Christian P. Kubicek of Vienna University of Technology (Austria) for their critical review of this manuscript.
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