Identification of TTAGGG-binding proteins in Neurospora crassa, a fungus with vertebrate-like telomere repeats
© Casas-Vila et al. 2015
Received: 20 June 2015
Accepted: 27 October 2015
Published: 17 November 2015
To date, telomere research in fungi has mainly focused on Saccharomyces cerevisiae and Schizosaccharomyces pombe, despite the fact that both yeasts have degenerated telomeric repeats in contrast to the canonical TTAGGG motif found in vertebrates and also several other fungi.
Using label-free quantitative proteomics, we here investigate the telosome of Neurospora crassa, a fungus with canonical telomeric repeats. We show that at least six of the candidates detected in our screen are direct TTAGGG-repeat binding proteins. While three of the direct interactors (NCU03416 [ncTbf1], NCU01991 [ncTbf2] and NCU02182 [ncTay1]) feature the known myb/homeobox DNA interaction domain also found in the vertebrate telomeric factors, we additionally show that a zinc-finger protein (NCU07846) and two proteins without any annotated DNA-binding domain (NCU02644 and NCU05718) are also direct double-strand TTAGGG binders. We further find two single-strand binders (NCU02404 [ncGbp2] and NCU07735 [ncTcg1]).
By quantitative label-free interactomics we identify TTAGGG-binding proteins in Neurospora crassa, suggesting candidates for telomeric factors that are supported by phylogenomic comparison with yeast species. Intriguingly, homologs in yeast species with degenerated telomeric repeats are also TTAGGG-binding proteins, e.g. in S. cerevisiae Tbf1 recognizes the TTAGGG motif found in its subtelomeres. However, there is also a subset of proteins that is not conserved. While a rudimentary core TTAGGG-recognition machinery may be conserved across yeast species, our data suggests Neurospora as an emerging model organism with unique features.
Linear chromosome ends of nearly all eukaryotes consist of repetitive telomeric sequences that protect the integrity of the genome. This function is assisted by interacting proteins, generally referred to as the telosome, which are essential for functional telomeres . In mammals, a protein complex consisting of six core members (TRF1, TRF2, RAP1, TIN2, TPP1 and POT1) protects the TTAGGG-repeat telomeres from recognition by the DNA damage repair machinery [2, 3] with TRF1 and TRF2 being direct double-strand telomere binding proteins and POT1 binding the TTAGGG single-strand overhang. Additionally, HOT1 was recently identified as a third direct double-strand telomere binding protein in mammals involved in telomerase-mediated telomere homeostasis .
While baker’s yeast (S. cerevisiae) and fission yeast (S. pombe) telomeres have been extensively studied and used as a model in telomere biology, they are both rather exceptional, as they feature degenerated telomeric repeats in juxtaposition to canonical repeat motifs found in most other eukaryotes. Furthermore, the telosomes of these two yeast model species differ greatly: In baker’s yeast, the degenerated double-strand repeats are recognized by Rap1 which interacts with Rif1 and Rif2 [5, 6], while in fission yeast the direct double-strand binding protein Taz1  anchors a telomeric multi-protein complex consisting of Taz1, Rap1, Poz1, Ccq1, Tpz1 and Pot1 .
Despite their potentially distinct telomere biology, considerable effort has been made to identify telomere binding proteins in these yeast species. While the identification of telomere binding proteins by their ability to bind telomeric repeats in purification experiments seems straightforward, this strategy has become even more applicable recently due to advances in protein identification by mass spectrometry. For instance, proteomic isolation of chromatin fragments (PICh), a reverse ChIP method, has been established on human telomeres . Similarly, classical DNA affinity purification was used to identify TRF1 by mass spectrometry  and the same technique in combination with SILAC has uncovered HOT1 as a direct telomeric DNA binding protein . The latter comparison also highlights how advances in proteomics have enabled the discovery of novel telomeric factors. Additionally, a quantitative telomeric chromatin isolation protocol (Q-TIP) based on immunopurification of TRF2 has been used to identify telomeric proteins . However, the known telomere binders in fungi have been identified by different means prior to streamlined applicability of proteomics. Rap1 was first identified based on its recombinant protein being similarly sensitive to protease digestion as the Telomere Binding Activity in S. cerevisiae and on both sharing similar size and specificity . Similarly, Taz1 has not been initially identified by its ability to bind telomeric repeats, but as a factor strongly enhancing telomere length in a genetic screen . More recently, a double-strand telomere binding protein in Yarrowia lipolytica was identified by sequence comparison focusing on its two myb domains similar to the human TRFs . We reasoned that an unbiased, proteome-wide interactomics approach would thus be beneficial to uncover the telosome in a not yet characterized fungus, as it allows the detection of telomeric proteins without assumptions and/or prior knowledge. We have previously demonstrated that we are able to identify direct telomere binding proteins in mouse and human  and here we apply single-step DNA affinity purification combined with quantitative proteomics to identify the direct telomere binding proteins in Neurospora crassa.
We chose Neurospora because little is known about the telosome of TTAGGG-repeat telomeric fungi, and it would be appealing to have a model organism that more directly relates to human telomeres in terms of sequence. Indeed, it was recently shown that while fungal and vertebrate telomerase RNAs share a common ancestral core, the N. crassa telomerase is more closely related to vertebrates . It is thus likely that its telomere binding proteins are more closely related to those of vertebrate species compared to the current yeast models. Here we investigated a comprehensive telosome for N. crassa and uncover proteins directly binding to TTAGGG repeats, providing a basis for further functional characterization of N. crassa telomere biology as an emerging model organism.
Results and discussion
Identification of the Neurospora telosome by quantitative proteomics
Evolutionary conservation of TTAGGG-associated proteins in fungi
As expected, we obtained the highest number of homologs (10 of 12) in the closely related fungus A. fumigatus. For around half of our candidates, we were still able to also identify homologs in the other three yeast species. While the proteins from C. albicans and A. fumigatus are not characterized, nearly all homologs in S. cerevisiae and S. pombe were already implicated in (sub)-telomere regulation, confirming that we indeed identified telomeric proteins by our quantitative mass spectrometry screen (Fig. 1b). NCU07735 is orthologous to the single-strand binding protein Tcg1 from S. pombe  and also has a weak homology to the telomeric ssDNA-binding protein Nsr1 of S. cerevisiae . We equally identified the homolog of the S. cerevisiae telomeric ssDNA-binding protein Gbp2  called NCU02404. Cross-confirming this finding, we also affinity-purified Gbp2 from a similar telomere pull-down screen using a degenerated telomeric repeat with S. cerevisiae extracts (unpublished data). NCU03416 is the homolog of the essential Tbf1 (TTAGGG-binding protein 1) in S. cerevisiae . Furthermore, NCU02182 is homologous to Tay1 , which can bind both the Yarrowia lipolytica telomeric sequence (GGGTTAGTCA) as well as TTAGGG repeats . Finally, NCU01991 has weak homology to Tbf1 and with the presence of a myb/homeobox domain (Fig. 2a) shows the common DNA-binding domain of telomeric proteins. To use comparable naming to yeast, we suggest to rename NCU03416 as Tbf1, NCU01991 as Tbf2, NCU02182 as Tay1, NCU02404 as Gbp2 and NCU07735 as Tcg1 in Neurospora.
Investigation of binding characteristics of the Neurospora proteins
Comparison of telomere associated proteins in fungi
The identification of a minimum of six TTAGGG double-strand binding proteins in Neurospora is unexpected. When we compare to TTAGGG binding proteins in the closely related telomere models only one protein (Tbf1) has been reported in S. cerevisiae and three (Tbf1, Tay1 and Taz1) in S. pombe / Y. lipolytica. Intriguingly, these three conserved proteins between S. pombe / Y. lipolytica and Neurospora all contain the classical myb/homeobox domain found also in the vertebrate telomere proteins TRF1, TRF2 and HOT1. While myb/homeobox domain proteins may be linked to core functions at the telomere, additional proteins may have been recruited in evolution to recognize TTAGGG repeats in a context in which they served as actual chromosome extremities. For instance, we here demonstrate that the zinc finger protein NCU07846 can also recognize these repeats and may represent an emerging class of telomere binding proteins, including ZSCAN4  and Ring1b . NCU07846 is the first zinc finger protein shown to directly bind TTAGGG repeats. The high number of TTAGGG-binding proteins in Neurospora may directly relate to the TTAGGG repeat being its telomeric sequence, requiring additional functionality in comparison to S. cerevisiae where the TTAGGG sequence is found in the subtelomere. It is furthermore possible that also in S. pombe and S. cerevisiae not all direct telomere binding proteins are yet known as similar systematic investigations based on affinity purification have not yet been reported for these yeast models.
We did not identify any homologs to the proteins directly binding to the degenerated telomeric repeats in S. cerevisiae and S. pombe in our screen. This might be the result of divergent evolution and it is therefore attractive to speculate that the degenerated repeat telomeres and their binding proteins are a specialization in these yeast species and that they co-exist with a rudimentary core TTAGGG-repeat binding machinery shared among all yeast species. Based on our knowledge of Neurospora telomere binding proteins, it is likely that Tbf1 end-capping in S. cerevisiae  is a remnant of an evolutionarily retained function. The additional TTAGGG- associated proteins Reb1, Env11, Vid22 in S. cerevisiae [26–28] likely reflect adaptations to the use of TTAGGG repeats as S. cerevisiae subtelomeres. It seems therefore likely that the canonical repeats such as TTAGGG are the actual ancestral core found in the vast majority of species in all clades and that the degenerated repeats in S. cerevisiae and S. pombe are a special telomeric situation in some fungi. Our data strongly supports the hypothesis that Tbf1 homologs in fungi originate from a common ancestor with a function in telomere regulation .
Our current experimental data sheds light on the evolutionary conservation of telomere-binding proteins and uncovers more TTAGGG-binding proteins in Neurospora as expected, suggesting it as an attractive new model species in telomere biology.
Preparation of Neurospora extracts
Neurospora crassa strain 74-OR23-1VA biomass was resuspended in PBB buffer (150 mM NaCl, 50 mM Tris–HCl pH 8.0, 10 mM MgCl2, 0.5 % Igepal CA630, 2 mM EDTA) and shock frozen in liquid nitrogen. The pellets were lysed by bead milling (MS400, Retsch) in a 25 ml stainless steel container and a steel ball at 30 Hz three times for 5 min. The sample was cooled in liquid nitrogen during the breaks. The frozen biomass was transferred and the melted sample was centrifuged for 1 min at 13,000 g. The supernatant was aliquoted and stored at −80 °C until usage. Protein concentration was determined by Bradford assay (Biorad).
The biotinylated (TTAGGG) and (TGTGAG) baits were prepared as previously described . Either 1 mg of Neurospora extract (MS experiment) or 200 μg of E. coli lysate containing recombinant protein (Western blot) were incubated with 500 μg Dynabeads Streptavidin (Life Technologies) coupled with either telomeric or control DNA for 1 h at 4 °C in PBB buffer (150 mM NaCl, 50 mM Tris–HCl pH 8.0, 10 mM MgCl2, 0.5 % Igepal CA630, Complete Protease Inhibitor without EDTA (Roche)) under slight agitation. In the case of the competition experiment increasing amounts (0 nmol, 0.01 nmols, 0.1 nmols, 0.2 nmols, 1 nmols, 2 nmols) of single-strand competitor (TTAGGG)10 or a non-specific 57 nt single-strand control oligonucleotide were added to the incubation. After three washing steps with PBB buffer, bound proteins were boiled in 1x LDS buffer containing 100 mM DTT (Life Technologies) at 70 °C and separated on NuPAGE Novex Bis-Tris precast gels (Life Technologies).
MS sample preparation
Each gel lane was cut into four slices and each slice was minced prior to transfer to an Eppendorf tube. The samples were destained in 50 % ethanol/25 mM ammonium bicarbonate pH 8.0 buffer twice for 30 min, reduced with 10 mM DTT at 56 °C and alkylated with 55 mM iodoacetamide in the dark. The gel pieces were incubated with MS-compatible trypsin (Promega) at 37 °C overnight. Peptides were eluted from the gel pieces using pure acetonitrile and extraction buffer (70 % acetonitrile/30 % ammoniumbicarbonate buffer pH 8/0.1 % TFA). After removal of the acetonitrile in a concentrator (Eppendorf) the peptides were loaded onto a C18 StageTip  to be stored.
Mass spectrometry measurement
The peptides in each fraction were separated by C18 reversed phase chromatography. The separation capillary was packed with Reprosil-Pur 120 C18-AQ, 3 μm (Dr. Maisch). The peptides were separated on a 145 min gradient from 5 to 40 % acetonitrile in 0.1 % formic acid using an EasyHPLC (Proxeon) coupled online to a LTQ Orbitrap XL mass spectrometer (Thermo Scientific). The mass spectrometer was operated in data-dependent acquisition mode performing top10 MS/MS (CID) per full scan (60,000 resolution, scan window 350–1750 m/z) controlled by the Xcalibur software.
The raw data was processed with MaxQuant  22.214.171.124 using the Neurospora version 3 database (9873 entries) with standard settings except the following (FastLFQ was deactivated and match between runs was activated). The results including LFQ (label-free quantitation) intensities were loaded into Perseus (version 126.96.36.199). Protein groups consisting of only identified by site proteins, reverse hits and contaminants were removed. Proteins that were detected in three of the four TTAGGG replicate measurements were considered. Missing values were imputed in each column separately by using a normal distribution with a width of 0.3 downshifted by 1.8 (standard values). To obtain the LFQ enrichment, the difference of the arithmetic mean of the quadruplicates of control and bait was calculated and plotted (x-axis). A two-sided t-test with 250 randomizations was performed between the four replicates of the telomeric bait against the four replicates of the control baits. A significance threshold was determined at an FDR of 0.05 and s0 = 0.2. The results were exported and plotted in R (version 188.8.131.52).
BLAST and evolutionary analysis
Annotated N. crassa peptide sequences of the 12 hits were blasted using the NCBI blastp (protein-protein BLAST) standard search. Searches were carried out against S. cerevisiae (taxid:4932), S. pombe (taxid:4896), A. fumigatus (taxid:746128) and C. albicans (taxid:5476). Blast hits were reverse blasted against N. crassa (taxid:5141) as search organism to verify homology. Homologs were only considered valid if both the forward and reverse blast yielded e-values smaller than e-8 and if in both directions the respective proteins were each the strongest hit. In some cases also weak homologs (e-values smaller than e-3) are reported, in which the forward and/or reverse blast identified a different protein as the direct homolog.
Cloning of Neurospora transcripts
Total RNA was extracted from N. crassa 74-OR23-1VA strain cells using the PrepEase RNA spin kit (USB) according to the manufacturer’s yeast protocol. The RNA was reverse transcribed using the protocol for polyadenylated mRNA of the First strand cDNA synthesis kit (Thermo). The transcripts of interest were amplified using chemically synthesized oligonucleotides (Metabion, Additional file 1: Table S3) and were cloned into pCR8-TOPO (Life Technologies) using TA cloning. Inserts were validated by Sanger sequencing prior to transfer into the pCoofy4 (N-terminal His-MBP-tag) expression vector using SLIC cloning . In cases were amplified sequences did not match to the Neurospora transcript prediction, a complete procedure was repeated starting from total RNA extraction of Neurospora cells.
Recombinant protein expression
The pCoofy4 expression vectors were transformed by heat-shock into E. coli BL21. Bacteria were grown overnight at 25 °C in auto-induction media  and harvested at an OD600 8–12 by centrifugation at 3000 g for 10 min at 4 °C. Cell pellets were resuspended in lysis buffer (100 mM NaCl, 50 mM Tris–HCl pH7.5, 10 mM MgCl2) to an OD600 of 6 and lysed by bead milling using 0.1 mm diameter Zirconia/glass-beads (Carl-Roth) in a tissue lyzer (Precellys) twice at 5600 rpm for 30 s. Cell debris was removed by centrifugation and protein concentration was determined by Bradford assay (Biorad). Successful expression and solubility of recombinant proteins were assessed by SDS-PAGE. Lysates were stored at −20 °C in 10 % glycerol.
Proteins were transferred to a Protran 85 nitrocellulose membrane (GE Healthcare) using a wet blot system in transfer buffer (20 % methanol, 25 mM Tris–HCl pH7.5, 150 mM glycine) at 300 mA for at least 90 min. The membrane was blocked and incubated with the Penta-His HRP conjugate kit (Qiagen) according to the manufacturer’s instructions. Individual bands were detected using ECL Detection Reagent (GE Healthcare) and visualized by a Biorad ChemiDoc System.
The data set supporting the results of this article is included within the article and its additional files.
We thank Brian Luke for critical reading of the manuscript, Astrid Klein for providing N. crassa biomass and Clara Bultmann, Sabine Suppmann and Claudia Strasser for help with recombinant protein expression. This work was supported by the Deutsche Forschungsgemeinschaft (grant number Bu2996/1-1), the Rhineland-Palatinate Forschungsschwerpunkt GeneRED, the National Research Foundation Singapore and the Singapore Ministry of Education under its Research Centres of Excellence initiative.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Jain D, Cooper JP. Telomeric strategies: means to an end. Annu Rev Genet. 2010;44:243–69.View ArticlePubMedGoogle Scholar
- Palm W, de Lange T. How shelterin protects mammalian telomeres. Annu Rev Genet. 2008;42:301–34.View ArticlePubMedGoogle Scholar
- de Lange T. How telomeres solve the end-protection problem. Science. 2009;326(5955):948–52.View ArticlePubMed CentralPubMedGoogle Scholar
- Kappei D, Butter F, Benda C, Scheibe M, Draskovic I, Stevense M, et al. HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment. EMBO J. 2013;32(12):1681–701.View ArticlePubMed CentralPubMedGoogle Scholar
- Marcand S, Wotton D, Gilson E, Shore D. Rap1p and telomere length regulation in yeast. Ciba Found Symp. 1997;211:76–93. discussion 93–103.PubMedGoogle Scholar
- Wotton D, Shore D. A novel Rap1p-interacting factor, Rif2p, cooperates with Rif1p to regulate telomere length in Saccharomyces cerevisiae. Genes Dev. 1997;11(6):748–60.View ArticlePubMedGoogle Scholar
- Cooper JP, Nimmo ER, Allshire RC, Cech TR. Regulation of telomere length and function by a Myb-domain protein in fission yeast. Nature. 1997;385(6618):744–7.View ArticlePubMedGoogle Scholar
- Dejardin J, Kingston RE. Purification of proteins associated with specific genomic Loci. Cell. 2009;136(1):175–86.View ArticlePubMed CentralPubMedGoogle Scholar
- Chong L, van Steensel B, Broccoli D, Erdjument-Bromage H, Hanish J, Tempst P, et al. A human telomeric protein. Science. 1995;270(5242):1663–7.View ArticlePubMedGoogle Scholar
- Grolimund L, Aeby E, Hamelin R, Armand F, Chiappe D, Moniatte M, et al. A quantitative telomeric chromatin isolation protocol identifies different telomeric states. Nat Commun. 2013;4:2848.View ArticlePubMedGoogle Scholar
- Longtine MS, Wilson NM, Petracek ME, Berman J. A yeast telomere binding activity binds to two related telomere sequence motifs and is indistinguishable from RAP1. Curr Genet. 1989;16(4):225–39.View ArticlePubMedGoogle Scholar
- Kramara J, Willcox S, Gunisova S, Kinsky S, Nosek J, Griffith JD, et al. Tay1 protein, a novel telomere binding factor from Yarrowia lipolytica. J Biol Chem. 2010;285(49):38078–92.View ArticlePubMed CentralPubMedGoogle Scholar
- Qi X, Li Y, Honda S, Hoffmann S, Marz M, Mosig A, et al. The common ancestral core of vertebrate and fungal telomerase RNAs. Nucleic Acids Res. 2013;41(1):450–62.View ArticlePubMed CentralPubMedGoogle Scholar
- Cox J, Hein MY, Luber CA, Paron I, Nagaraj N, Mann M. Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ. Mol Cell Proteomics. 2014;13(9):2513–26.View ArticlePubMed CentralPubMedGoogle Scholar
- Pang TL, Wang CY, Hsu CL, Chen MY, Lin JJ. Exposure of single-stranded telomeric DNA causes G2/M cell cycle arrest in Saccharomyces cerevisiae. J Biol Chem. 2003;278(11):9318–21.View ArticlePubMedGoogle Scholar
- Lin JJ, Zakian VA. Isolation and characterization of two Saccharomyces cerevisiae genes that encode proteins that bind to (TG1-3)n single strand telomeric DNA in vitro. Nucleic Acids Res. 1994;22(23):4906–13.View ArticlePubMed CentralPubMedGoogle Scholar
- Brigati C, Kurtz S, Balderes D, Vidali G, Shore D. An essential yeast gene encoding a TTAGGG repeat-binding protein. Mol Cell Biol. 1993;13(2):1306–14.View ArticlePubMed CentralPubMedGoogle Scholar
- Visacka K, Hofr C, Willcox S, Necasova I, Pavlouskova J, Sepsiova R, et al. Synergism of the two Myb domains of Tay1 protein results in high affinity binding to telomeres. J Biol Chem. 2012;287(38):32206–15.View ArticlePubMed CentralPubMedGoogle Scholar
- Ding J, Hayashi MK, Zhang Y, Manche L, Krainer AR, Xu RM. Crystal structure of the two-RRM domain of hnRNP A1 (UP1) complexed with single-stranded telomeric DNA. Genes Dev. 1999;13(9):1102–15.View ArticlePubMed CentralPubMedGoogle Scholar
- Kamma H, Fujimoto M, Fujiwara M, Matsui M, Horiguchi H, Hamasaki M, et al. Interaction of hnRNP A2/B1 isoforms with telomeric ssDNA and the in vitro function. Biochem Biophys Res Commun. 2001;280(3):625–30.View ArticlePubMedGoogle Scholar
- Court R, Chapman L, Fairall L, Rhodes D. How the human telomeric proteins TRF1 and TRF2 recognize telomeric DNA: a view from high-resolution crystal structures. EMBO Rep. 2005;6(1):39–45.View ArticlePubMed CentralPubMedGoogle Scholar
- Balk B, Maicher A, Dees M, Klermund J, Luke-Glaser S, Bender K, et al. Telomeric RNA-DNA hybrids affect telomere-length dynamics and senescence. Nat Struct Mol Biol. 2013;20(10):1199–205.View ArticlePubMedGoogle Scholar
- Zalzman M, Falco G, Sharova LV, Nishiyama A, Thomas M, Lee SL, et al. Zscan4 regulates telomere elongation and genomic stability in ES cells. Nature. 2010;464(7290):858–63.View ArticlePubMed CentralPubMedGoogle Scholar
- Bartocci C, Diedrich JK, Ouzounov I, Li J, Piunti A, Pasini D, et al. Isolation of chromatin from dysfunctional telomeres reveals an important role for Ring1b in NHEJ-mediated chromosome fusions. Cell Rep. 2014;7(4):1320–32.View ArticlePubMed CentralPubMedGoogle Scholar
- Berthiau AS, Yankulov K, Bah A, Revardel E, Luciano P, Wellinger RJ, et al. Subtelomeric proteins negatively regulate telomere elongation in budding yeast. EMBO J. 2006;25(4):846–56.View ArticlePubMed CentralPubMedGoogle Scholar
- Bonetti D, Anbalagan S, Lucchini G, Clerici M, Longhese MP. Tbf1 and Vid22 promote resection and non-homologous end joining of DNA double-strand break ends. EMBO J. 2013;32(2):275–89.View ArticlePubMed CentralPubMedGoogle Scholar
- Chasman DI, Lue NF, Buchman AR, LaPointe JW, Lorch Y, Kornberg RD. A yeast protein that influences the chromatin structure of UASG and functions as a powerful auxiliary gene activator. Genes Dev. 1990;4(4):503–14.View ArticlePubMedGoogle Scholar
- Krogan NJ, Cagney G, Yu H, Zhong G, Guo X, Ignatchenko A, et al. Global landscape of protein complexes in the yeast Saccharomyces cerevisiae. Nature. 2006;440(7084):637–43.View ArticlePubMedGoogle Scholar
- Teixeira MT, Gilson E. Telomere maintenance, function and evolution: the yeast paradigm. Chromosome Res. 2005;13(5):535–48.View ArticlePubMedGoogle Scholar
- Rappsilber J, Mann M, Ishihama Y. Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips. Nat Protoc. 2007;2(8):1896–906.View ArticlePubMedGoogle Scholar
- Cox J, Mann M. MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification. Nat Biotechnol. 2008;26(12):1367–72.View ArticlePubMedGoogle Scholar
- Scholz J, Besir H, Strasser C, Suppmann S. A new method to customize protein expression vectors for fast, efficient and background free parallel cloning. BMC Biotechnol. 2013;13:12.View ArticlePubMed CentralPubMedGoogle Scholar
- Studier FW. Protein production by auto-induction in high density shaking cultures. Protein Expr Purif. 2005;41(1):207–34.View ArticlePubMedGoogle Scholar