- Research article
- Open Access
De novo transcriptome assembly for the lobster Homarus americanus and characterization of differential gene expression across nervous system tissues
BMC Genomicsvolume 17, Article number: 63 (2016)
The American lobster, Homarus americanus, is an important species as an economically valuable fishery, a key member in marine ecosystems, and a well-studied model for central pattern generation, the neural networks that control rhythmic motor patterns. Despite multi-faceted scientific interest in this species, currently our genetic resources for the lobster are limited. In this study, we de novo assemble a transcriptome for Homarus americanus using central nervous system (CNS), muscle, and hybrid neurosecretory tissues and compare gene expression across these tissue types. In particular, we focus our analysis on genes relevant to central pattern generation and the identity of the neurons in a neural network, which is defined by combinations of genes distinguishing the neuronal behavior and phenotype, including ion channels, neurotransmitters, neuromodulators, receptors, transcription factors, and other gene products.
Using samples from the central nervous system (brain, abdominal ganglia), abdominal muscle, and heart (cardiac ganglia, pericardial organs, muscle), we used RNA-Seq to characterize gene expression patterns across tissues types. We also compared control tissues with those challenged with the neuropeptide proctolin in vivo. Our transcriptome generated 34,813 transcripts with known protein annotations. Of these, 5,000-10,000 of annotated transcripts were significantly differentially expressed (DE) across tissue types. We found 421 transcripts for ion channels and identified receptors and/or proteins for over 20 different neurotransmitters and neuromodulators. Results indicated tissue-specific expression of select neuromodulator (allostatin, myomodulin, octopamine, nitric oxide) and neurotransmitter (glutamate, acetylcholine) pathways. We also identify differential expression of ion channel families, including kainite family glutamate receptors, inward-rectifying K+ (IRK) channels, and transient receptor potential (TRP) A family channels, across central pattern generating tissues.
Our transcriptome-wide profiles of the rhythmic pattern generating abdominal and cardiac nervous systems in Homarus americanus reveal candidates for neuronal features that drive the production of motor output in these systems.
An overarching goal in the study of neurobiology is to translate the capacity to perform an action to its underlying cellular and molecular mechanisms. As a result, nervous systems involved in central pattern generation have become a well-studied model for the control of behavior [1, 2]. Central pattern generators are neural networks that produce repetitive actions such as the beating of the heart or gait patterns in walking or running. They are defined by their ability to produce rhythmic motor patterns even in the absence of sensory feedback . The continuous, measurable physical output of these networks allows for straightforward comparisons between actual behavior and network activity. Moreover, these networks perform complex motor tasks in a coordinated, rhythmic fashion while still adapting the behavior to environmental contingencies [3, 4]. The accessibility of these networks combined with this juxtaposition between precision and flexibility has sparked decades of research on the subject, especially in invertebrate models like the American lobster Homarus americanus where these networks are relatively simple (tens or hundreds, rather than thousands, of neurons) compared to their vertebrate counterparts.
In this study, we utilize the American lobster Homarus americanus for its role as a model organism in the study of neural networks, though it is also an economically valuable fishery and an important species in marine ecosystem dynamics. To perform this work, we de novo assemble a transcriptome for this species, which has no published genome or transcriptome to date. This genetic resource will expand our ability to study this species in both a physiological and ecological context, as well as within the framework of neurobiology and central pattern generation.
In the lobster, perhaps the simplest central pattern generating network (CPG) is the cardiac system that controls the rhythmic pumping of the neurogenic heart and distributes hemolymph (or ‘blood’) throughout the body (Fig. 1a). This system includes the continuously bursting cardiac ganglion (neural control center) and motor neuron connections embedded within the heart itself . The cardiac system generates the heartbeat in the frequency range of 0.5-1.5 Hz . Cardiac performance can be influenced by external factors (including temperature and temperature acclimation) [6–8], and by release of neuromodulators from the pericardial organ . The pericardial organ is an important member of both the cardiac and endocrine systems ; it is a neurosecretory structure attached to the lateral walls of the pericardial cavity that releases neuromodulators through nerve trunks with dense assemblies of release terminals [5, 11]. These nerves release neuromodulators as hormones into the hemolymph for distribution to other networks and also innervate the heart to directly modulate the cardiac ganglion [12, 13].
Another well-studied model for central pattern generation in the lobster is the abdominal ganglion, which contains several CPG networks that coordinate locomotion and rhythmic escape swimming behaviors and also play a role in posture [14–16]. This system is composed of six abdominal ganglia, one for each abdominal segment, located in the ventral nerve cord. These ganglia control the swimmeret muscles that operate the fin-like swimmerets (located on the ventral side of abdomen) used in locomotion, righting, and ventilation, the slow extensor-flexor muscles used during backward walking, and the large flexor–extensor muscles used for rhythmic escape swimming [14–23]. The abdominal system controls both phasic and tonic muscle fibers and thus, by comparison, can operate in time domains slower and more rapid than the cardiac system [24, 25]. It can maintain ‘background activity’ in maintenance of posture and also generate rapid tail flips or swimmeret beating at frequencies up to 5 Hz [24, 26].
Characterizing the function and connectivity of these circuits led to a control architecture for innate behavior: the command neuron, coordinating neuron, central pattern generator model. These CPG networks are characterized by a particular ability to respond to external and internal variables (temperature, pH) while maintaining the stable performance [1, 2, 7, 27, 28]. It has also led to breakthroughs in our overall understanding of the role of neuromodulation in shaping the activity of neural circuits [29, 30]. Neuromodulators are signaling molecules that act in concert to modify the intrinsic firing properties of neurons, and can transform the functional connectivity of neural circuits and alter their output. Neural activity can be induced, modified, or terminated by input from multiple neuromodulators [30, 31]. Through extensive work, researchers have identified dozens of neuromodulators in crustacean decapods and characterized their ability to alter circuit dynamics in vitro and, in some cases, motor activity or behavior in vivo . Neuromodulators can be released both intrinsically (from a cell within the circuit) and extrinsically (from another area of the nervous system, such as the neurosecretory pericardial organ) (Fig. 1a). However, despite evidence of systemic release of neuromodulators, they are not ubiquitous within the nervous system—immunocytological work and, recently, a characterization of the H. americanus peptidome across tissue types suggests localized distribution of neuromodulators . Exploring the full specificity of expressed neuromodulators and, particularly, their receptors across nervous system tissues is an important next step towards a more complete understanding of the complex interplay of neuromodulators in functional motor networks.
Accordingly, the activity of a neural network is not just conditional to modulatory inputs, but also to the response of the participating neurons to these inputs. The response is dictated by intrinsic properties of those neurons—the number and kind of ion channels on the membrane . Thus, recent focus has shifted to answering the question: what genetic constructs underlie the production of these stereotyped motor patterns? Current theoretical and molecular research demonstrates correlations between gene expression of different ion channel proteins are actively regulated to maintain robust neuronal output [34–40]. In the invertebrate CPGs the stomatogastric and cardiac ganglia, despite variable expression levels in a particular ion channel, there exist characteristic sets of correlated expression of these genes. The relationships between potassium channels (shal IA, shab IKd, shaw IKd, shaker IA and BKKCa IK[Ca])) and membrane conductances in identified cell types are particularly well described [34–36, 39]. Though these features are only a small portion of the range of factors governing the identity of these networks, they do speak to the overall trend in opinion that neuronal identity is not defined by the expression of unique genes, but by specific combinations of genes .
In this study, we characterize the transcriptional profiles of two types of nervous system tissues—motor, sensory, and command neuron tissue from the abdominal ganglia and supraesophogeal ganglia (or ‘brain’), and hybrid neural/muscle tissue from the heart. We characterize these central nervous system (CNS) and ‘hybrid’ heart tissues against muscle tissue and against each other to address representative transcriptomic signatures of neural tissue types. Finally, we compare abdominal ganglia to the heart tissues to target transcriptome-wide differences between these two central pattern generating tissues.
These experiments also explore the role of a circulating neuromodulator in the transcriptional regulation of ion channels by including a hormonal treatment with proctolin. Proctolin is an endogenous pentapeptide that acts as an excitatory neuromodulator . It was selected for this study because its physiological role as a neuromodulator is well characterized. Proctolin can increase the frequency of action potentials, increase the amplitude of muscle contraction, and initiate activity in quiescent systems [43–48]. It is also accepted to function at a system-wide hormonal level, a context in which this neuropeptide remains poorly understood [49, 50]. Here, we investigate the role of proctolin as a hormonal regulator of gene expression.
The experiments in this study were performed by sampling three types of tissue: muscle (abdominal muscle, n = 3), neural (abdominal nerve cord, n = 3; supraesophogeal ganglion, n = 1), and neuromuscular hybrid (heart, n = 3). Individuals used were treated with three daily exogenous proctolin injections to temporarily raise hemal concentrations of this neuromodulator in vivo to 10−6M (treated, n = 5) or injection of physiological saline (control/untreated, n = 5). The effects of increased hemal proctolin across treated and untreated tissues are limited and addressed briefly. All other analyses are conducted bioinformatically controlling for the effects of this treatment in our differential gene expression analysis, and reflect only changes in tissue type (Additional file 1: Table S1). For analyses of tissue type we include both central pattern generating abdominal ganglia and descending inputs (brain) to characterize the differences between these CNS tissues and muscle or heart tissues. However, for direct comparison of the abdominal and cardiac networks, differential gene expression analysis was re-run with only the abdominal ganglia and heart samples.
By employing a transcriptome-wide approach to investigate neural tissue types and examine two central pattern generating networks, we aim to distinguish combinations of factors involved in determining neuronal identity and function of these systems, including ion channels, neurotransmitters, neuromodulators, receptors, and other gene products.
Results and discussion
De novo transcriptome assembly and annotation
To date, there remains no published genome for Homarus americanus. Thus, for this study, we de novo assembled and blast annotated a reference transcriptome for H. americanus in Trinity using 119.7 million reads from four tissue types (heart, abdominal muscle, abdominal nerve cord, brain) stemming from four lobsters. The assembly resulted in a transcriptome of 115,757 contigs, with an N50 of 1,289. The maximum and minimum contig lengths were 17,481 and 201 bp, respectively, with approximately 25 % of the contigs exceeding 1,000 bp in length. tBlast against NCBI and UniProtKB’s Swiss-Prot/TrEMBLE databases resulted in reliable protein annotations (e value < 10−4) for 34,813 contigs, or approximately 30 % the assembled contigs. We observed the N50 in annotated (1909bp) and unannotated (773bp) transcripts varied in length, suggesting that larger transcripts were more likely to have lower blast e-values and thus better protein annotations.
Of our 34,813 annotated transcripts, the annotations contained 12,389 unique proteins, an approximation determined by redundancies in Entrez Gene IDs. Mapping Entrez Gene IDs to Gene Ontology (GO) annotations identified 11,383 GO categories represented in our transcriptome, including 7,161 gene products attributed to biological processes, 1,090 attributed to cellular components, and 3,132 attributed to molecular function. The GO terms attributed to the greatest number of genes, in descending order, were nucleus (GO:0005634), cytoplasm (GO:0005737), protein binding (GO:0005515), integral component of membrane (GO:0016021), membrane (GO:0016020), metal ion binding (GO:0046872), and plasma membrane (GO:0005886).
Differences in expression between all tissue types and treatment groups
Samples were visualized using a principal component analysis and sample-to-sample distances as data quality assessment and also to visualize relative relatedness between samples (Fig. 1b). Principal component analysis and sample-to-sample distances (Additional file 2: Figure S1) display a closer relation of transcripts within a given tissue type than within a hormone treatment group. Principal component analysis also closely clusters the two types of neural tissues represented, the supraesophogeal (brain) and abdominal ganglia (hereafter together referred to as CNS nervous system or nerve tissues). In Additional file 2: Figure S1, a simple representation of Euclidian distances demonstrates nervous system tissues are farther from muscle and heart tissues than muscle and heart tissues are from each other.
Differential expression between treated and untreated tissues
Three daily treatments elevating hemal levels of the neuropeptide proctolin resulted in significant differential expression (adj p < 0.05) of 255 transcripts. Of these transcripts, 79 had reliable protein annotations (31 %). Gene ontology (GO) enrichment analysis did not identify significantly overrepresented (SO) GO categories (adjusted p value < 0.05, BH method) in treated tissues. Of the annotated transcripts, 80 % were upregulated and largely included proteins involved in immune and neural systems. Neural transcripts included innexin, proteins involved in the gap junctions between electrically connected neurons (adj p < 0.05, +3 log2fold change), and transient receptor potential channels (TRPA1, TRP pyrexia) (adj p < 0.05, +3 log2fold change). The greatest log2fold change was observed in three transcripts annotating to anti-lipopolysaccharide factors (ALFs) (adj p < 0.001, +5 log2fold change), a peptide with potent anticoagulation and antimicrobial abilities.
ALFs have been identified in the lobster and other crustaceans as part of the innate immunity of these organisms, a system that largely takes place in the hemocytes where antimicrobial compounds are synthesized and stored for release into the hemolymph [51–53]. These results indicate a relationship between hemal proctolin and increased synthesis of antimicrobial proteins.
Though an immune response could have been evoked by the injections themselves, we believe the immune response is likely not an artifact of treatment given the untreated and treated lobsters were injected in the same method with either sterile physiological saline or an identical solution containing synthetic proctolin, respectively. Moreover, the connection between another neuromodulator, octopamine, and immune responses is well documented in invertebrate systems . Octopamine increases total hemocyte count and nodule formation, enhances inositol trisphosphate (IP3) production in hemocytes, and increases phagocytosis [55–57]. The neuromodulator serotonin (5-Hydroxytryptamine) is also known to regulate phagocytosis, hemocyte production, and nodule formation [56–58]. The ability of octopamine to regulate immune responses is mediated by G-protein coupled receptors (GPCRs) and second messenger systems. The identified receptor for proctolin is also a GPCR, suggesting a similar mechanism by which proctolin may regulate immune response . Here we see hemal proctolin levels upregulate the transcription of ALFs, as well as a stress activated protein kinase identified in another crustacean immune response [UniprotKB: G0ZJ53] (adj p < 0.001, +4 log2fold change) . These results implicate a novel role for hemal proctolin in innate immunity pathways, and suggest this neuromodulator may be multi-faceted in its role as a signaling molecule and act on both neural and immune systems.
Differential expression between heart and muscle tissues
We found 15,046 transcripts with significant differential expression (adj p < 0.05) in heart tissues when compared to abdominal muscle tissues; of these differentially expressed (DE) transcripts, 32 % had reliable protein annotations (Table 1). GO enrichment analysis indicated 11 SO categories in heart tissues. The SO categories included extracellular region (GO:0005576), myoblast fusion (GO:0007520), actin binding (GO:0003779), structural constituent of muscle (GO:0008307), calcium ion binding (GO:0005509), and response to heat (GO:0009408).
Differential expression between central nervous system and muscle tissues
Results indicate 28,479 DE transcripts (adj p < 0.05) in CNS tissues (abdominal and supraesophogeal ganglia) compared to muscle tissues; 35 % annotated and included 4,755 unique genes. These genes comprised 22 significantly overrepresented GO categories (adj p <0.05), including plasma membrane (GO:0005886), cell adhesion (GO:007155), extracellular region (GO:005576), axon guidance (GO:007411), synapse (GO:0045202), and neurotransmitter secretion (GO: 0007269).
Differential expression between central nervous system and heart tissues
We found 20,179 DE transcripts between nerve and heart tissues, including 7,388 with protein annotations (37 % of all DE transcripts), 3,797 unique genes, and 38 significantly overrepresented GO categories (p < 0.05). Enriched GO categories included plasma membrane (GO:0005886), structural constituent of ribosome (GO:0003735), axon guidance (GO:0007411), extracellular matrix (GO: 0031012), and translation (GO: 0006412).
To address which genes were consistently DE in the neural and heart tissues compared to muscle tissues, we constructed a venn diagram (Fig. 2). Overlap of DE transcripts identified 6,771 transcripts differentially expressed across both contrasts and 2,069 DE across all three contrasts; these groups included many of the nervous system genes described in more detail below.
Regulation of neurotransmitters and neuromodulators
The nervous system of crustaceans is rich in neuromodulatory substances that act as extrinsic and intrinsic regulators of nervous system activity. To date, over two dozen of these neuroactive molecules have been identified . Our goal was to assess the presence and relative expression of these known crustacean neurotransmitters and neuromodulators across neural and non-neural tissues. Proteins involved in signaling pathways were detected for 20 different neuroactive substances, including known crustacean neurotransmitters (acetycholine, glutamate, serotonin, dopamine) and neuromodulators allostatin (AST), FLRFamide, FMRFamide, cardioactive peptide (CCAP), octopamine, orcokinin, dopamine, etc. These proteins included neuropeptides, receptors, and enzymes involved in neurotransmitter pathways (i.e. synthesis). As expected, most of these genes were upregulated in the nervous system tissues compared to muscle tissues (Table 2). However, some neuromodulators exhibited tissue-specific expression signatures for particular neuromodulator sequences or receptors (Fig. 3). For instance, the neuropeptide myomodulin was DE and downregulated in nervous tissue compared to both muscle (p < 0.05, −7 log2fold change) and heart (p <0.05, −6 log2fold change) tissue, suggesting a localized role in neuromuscular signaling. There was also a bifurcation of regulation of neuronal nitric oxide synthase (NO) across nerve and muscle tissues, suggesting the proteins or protein isoforms involved in NO signaling differ across tissues. NO is of particular interest as it has recently been shown to gate the polarity of endocannabinoid-modulation to shift the excitation-inhibition balance of a synapse and thus mediate long-term potentiation of rhythmic locomotor circuits [62–65]. All six transcripts for the carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein (70729) were downregulated in nerve tissue compared to muscle, and three of the six were DE (p < 0.05). Three nostrin (115677, 521834) transcripts were DE and downregulated (−5 log2fold change or greater) across the tissue types, but five other nostrin transcripts were DE and upregulated (+2 log2fold change or greater).
In comparing the heart tissue (which contains both muscle and the cardiac ganglion nervous system) with the other nervous tissues (abdominal chain and brain), there was a contrast in the expression of different octopamine receptors. Octopamine receptors beta 1-R, 2-R, and 3-R were upregulated in other nervous system tissues, compared to heart tissues (p value, ns, +2 log2fold change or greater). In contrast, both octopamine receptors transcripts matching UniProtKB Q25188.1 of Heliothis virescens were downregulated (1 of 2 transcripts DE, p value < 0.05, −2 log2fold change) and thus more highly expressed in cardiac tissue (Fig. 3). Octopamine β-receptors are a group of receptors homologous with vertebrate β-adrenergic receptors . These receptors respond to octopamine by increasing intracellular cAMP but display disparate pharmacological profiles, suggesting differences in sequences between these receptors results in varying functional roles in signaling activities [66, 67]. Our data provide context for localization of expression and expression levels in H. americanus, and suggest differences in receptor sequences for octopamine (as well as other signaling molecules) may play a role in defining specificity in targets and effects of these molecules as a circulating neuromodulators.
Transcription Factors for Neuronal Differentiation
To identify candidates for transcription factors involved in the differentiation of our neuronal tissues, we performed a blast analysis for transcription factors identified in terminal selector programs, or pathways that that control the expression of identifying features of mature individual neuron types [33, 68–72]. We searched for 24 different gene sequences, all of which were found within our transcriptome (e-val < 10−4) (Additional file 1: Table S2). Of these sequences, we found several transcription factors with a high proportion (>40 %) of transcripts DE and upregulated in our nervous system tissues, including ceh-36 the Otx-type homeobox gene involved in chemosensory neuron differentiation, ttx-1 an Otx-type transcription factor involved in thermosensory neuron identity, and ets-5 an ETS domain transcription factor involved in CO2/O2 sensory neurons .
We also blasted our transcriptome for the homeodomain transcription factor Shox2, which has been linked to excitatory interneurons in the rhythmic pattern generating kernel for spinal locomotion in mice . The top hit (comp32264_c0_seq2) had a 99 % sequence identity match to Shox2 and was DE and upregulated in heart tissues compared to muscle tissue (adj p < 0.05, +6 log fold change) and downregulated in nerve tissues compared to heart tissues (adj p < 0.05, −7 log fold change), indicating this transcription factor is highly expressed in our cardiac network compared to the abdominal ganglia, brain, or muscle.
Candidate genes for differentiation between abdominal and cardiac CPGs
To identify divergent genetic signatures across the abdominal and cardiac central pattern generating networks, we conducted a different gene expression analysis of just abdominal nerve cord tissues (n = 3) over heart tissues (n = 3) and then examined transcripts involved in neuronal identity.
In summary, 14,955 transcripts were differentially expressed; 38 % of transcripts had protein annotations. Gene ontology analysis identified 31 significantly enriched GO categories (p < 0.05) (Fig. 4). Many of these results, like the structural constituent of muscle (GO:0008307) and respiratory chain (GO:0070469), were downregulated in the abdominal nerve cord and are attributable to differences in nervous system tissue versus mixed muscle/nervous system tissue (Additional file 1: Table S3). Thus our analysis focused on GO categories and genes involved in neuronal differentiation with the goal of addressing the differences in the CPG nervous systems of the cardiac ganglion and abdominal ganglia.
Enriched GO category terminal bouton (GO:0043195) identified a role in kainite family glutamate receptors (GluK) in differentiation of these CPGs. GluK 2 receptors are DE and upregulated in abdominal ganglia (GluK2 p <0.001, +6 log2fold change) whereas GluK 3 and GluK 5 are DE and downregulated (respectively, GluK3 p <0.001, −10 log2fold change and GluK5 p < 0.05, −5 log2fold change). We also employed previous techniques for identifying neuromodulators, ion channels, and receptors (Figs. 5 and 6). Overall, this analysis detected 421 transcripts annotating to membrane ion channels or receptors. Results indicated cardioactive peptide (CCAP) is upregulated (p < 0.001, +9 log2fold change) in the abdominal system compared to the heart. This result is particularly interesting given the nerve terminals in the heart tissues from the neurosecretory pericardial organ; the expression of CCAP was first discovered in the pericardial organs, hence the etymology . Here, CCAP is indeed expressed in the heart tissues, but has relatively greater expression in the abdominal system. Also upregulated in the abdominal system were two variants of allostatin annotating to allatostatin-cc (Nilaparvata lugens) (p < 0.001, +7 log2fold change) and C-type preproallatostatin (Pandalopsis japonica) (p < 0.001, +6 log2fold change) and the neuromodulator red pigment containing hormone (RPCH) (p < 0.05, +4 log2fold change) (Fig. 5). Several contigs of acetylcholine pathway proteins were also upregulated in the abdominal CPG including subunit alpha 6 (p < 0.001, +10 log2fold change) and acetylcholinesterase (p < 0.001, +7 log2fold change). Myomodulin A (p < 0.001, −5 log2fold change) and octopamine receptors (p < 0.05, −2 log2fold change) were downregulated in the abdominal system compared to the cardiac system. Collectively, these results indicate CCAP, AST, RPCH, GluK2, and acetylcholine as strong candidates for the function of abdominal networks, and GluK3, GluK 5, myomodulin and octopamine as signatures of the cardiac network.
We observed all voltage gated L-type Ca2+ channel subunits and Ca2+ channel subunit alpha-2/delta-1 are DE and upregulated in the abdominal ganglia (Table 3). We also observed glutamate-gated Cl− channels (GLUCL) and calcium-activated Cl− channels (CLCA) were largely DE and upregulated, with log2fold changes of many transcripts greater than +5 (Fig. 6). K+ channels slo-1/slowpoke are also DE and upregulated. These large-conductance, big potassium or ‘BK’ channels are involved in calcium-dependent K+ currents (IKCa), participate in repolarization of the presynaptic terminal, and are important to the timing of action potentials [75–77]. Interestingly, recent studies in Drosophila melanogaster with slowpoke knockout mutants and RNAi have identified timing deficiencies in rhythmic motor patterns and, particularly, a drastically decreased ability for slo mutants to initiate rhythmic flight activity [78, 79]. Taken with our observed expression increases in the abdominal system, future studies could explore whether the slo K+ channel is a key constituent in intermittently active, rather than continually active, rhythmic motor generating nervous systems.
Ion channels that were DE and downregulated in this comparison, thus displaying significantly increased expression in cardiac systems, identified a predicted Cl− channel and two inward-rectifying K+ (IRK). IRK channels are important to neuronal excitability and the timing of neuronal activity [80, 81]. Combined, these observations suggest families of channels critical to the function and timing of motor pattern generating circuits including IRK channels for the heart and slo family BK channels and CLCA channels for the abdominal system. For a full list of differentially expressed ion channels, please refer to Additional file 1: Table S4.
Heart tissue in response to heat
An unexpected result of this study was the identification of significant overrepresentation of thermosensory genes in the heart tissues. Interestingly, in GO enrichment analysis of heart vs. muscle tissues the only significantly overrepresented biological process was ‘response to heat’ (GO:0009408), with over 50 % of involved genes differentially expressed (18 of 32 genes in category, p < 0.05). For visualization of enriched biological processes in heart tissues, please refer to Additional file 3: Figure S2.
As mentioned, central pattern generators are characterized by their ability to maintain robust output across changing internal and environmental factors [27, 82]. For poikilotherms, this ability is particularly important given there are no internal mechanisms for regulating temperature—an environmental factor with undeniable effects on all biological processes. The thermal behavior of H. americanus and other decapod crustaceans is well documented. They are able to respond to small changes in temperature (<0.5° C) and maintain rhythmic nervous system function over large temperature ranges [8, 82–84]. Moreover, these organisms are thermotactic and navigate toward preferred temperatures to optimize physiological function [85–87]. Our results may indicate candidate genes for this precise response to temperature in the cardiac and central nervous system tissues (Table 4). Of the 18 DE genes involved in heat response, two were DE and downregulated in all contrasts: heat shock 18 kDa protein and protein Efl21. Additionally, TRP channel pyrexia, locomotion defects protein, and hippocampal cholinergic neurostimulating peptide, were DE and upregulated in both heart and nervous system tissues when compared to muscle tissues.
In particular, this analysis identified two transient receptor potential (TRP) channel genes, pyrexia and painless, that belong to TRP A family (Table 2). Full analysis of our transcriptome identified 5 subtypes of TRP channels including three types of TRP A family genes (TRPA1, pyrexia, and painless), TRP M2 and TRP M3. TRP channels are a diverse family of channels permeable to Na+ and/or Ca2+ and involved in many varied types of sensory reception. Of the TRP channels identified in our transcriptome, research in other models establishes TRP M3 as an osmoreceptor, M2 is a possible oxidant stress sensor, and TRP A genes in temperature sensing . TRP A pyrexia and painless may be responsible for sensing different temperature ranges [89–92]. The differential expression of these channels in our samples, the upregulation of pyrexia in heart tissues and the upregulation of painless in CNS tissue, suggests a possible mechanism by which these systems are able to efficiently sense and react to temperature changes.
Our study detected tissue-specific patterns of increased expression of neuromodulators in the heart (octopamine), muscle (myomodulin), and nervous system (RPCH, AST, tachykinin, FLRFamide), as well as tissue-specific variation in expression sequences for octopamine and NO. We identified several neuronal factors contributing to the identity of our abdominal and cardiac systems. For the abdominal network, we observed significantly increased expression of acetylcholine receptors, GluK2, voltage gated L-type Ca2+ channels, calcium-activated Cl− channels, and K+ channels slo-1/slowpoke, and significantly increased expression of neuromodulators CCAP and AST. For the cardiac network, we observed increased expression of the neuromodulators myomodulin and octopamine, and GluK3, Gluk5, and IRK channels. We identified a set of neuronal differentiation transcription factors in these systems, including ceh-36, ttx-1, ets-5 and Shox2. This research also elucidated a novel role for the neuromodulator proctolin in regulating immune responses, and identified an overrepresentation of response to heat in the heart tissues of the lobsters, and suggests a possible mechanism for thermoreception in this poikilotherm stemming from thermosensory TRP A channels in the nervous system tissues.
Sample collection and preparation
Adult Homarus americanus were purchased from local commercial fisherman (F/V Jacqueline Bess, Nahant Fish and Lobster, Nahant, MA) and held in ambient running seawater tanks (10.29 ± 0.01 °C) at the Northeastern University Marine Science Center. Lobsters were not fed prior to experimentation. Four different tissue types were collected from live Homarus americanus: (1) abdominal nerve cord (n = 3), (2) supraesophogeal ganglion (the “brain”) (n = 1), (3) heart and pericardial cavity with neurosecretory pericardial organ (n = 3), (4) muscle tissue from abdominal muscles (n = 3). Prior to dissection, individuals were subjected to once-daily hormone treatments of the proctolin (Arg-Tyr-Leu-Pro-Thr) (injection of proctolin in physiological saline) or control treatments (physiological saline injection) for a three-day period. Hormone treatments served to temporarily increase systemic proctolin concentration to 10−6 M, based on standard hemolymph/bodyweight calculations  and physiologically relevant concentration levels [13, 45, 47]. Efficacy of injections increasing systemic proctolin was confirmed by quantitative mass spectrometry. The period of treatment was selected based on time frames of ion channel turnover, a process that can take hours to days [94–98]. Samples were removed with forceps and surgical scissors, flash-frozen in TRI® Reagent with liquid nitrogen, and pulverized with RNase-DNase-free pestles (VWR, Radnor, PA). We used Agilent Bioanalyzer 2100 to QC total RNA before library preparation, and visually inspected the RNA peaks and fluorescence units. To prep samples for RNA-Seq on the Illumina platform, mRNA was separated from the extracted Total RNA with Dynabeads® Oligo(dT)25 (Invitrogen Life Technologies, Grand Island, NY). cDNA libraries were constructed using the Vollmer laboratory protocol for non-genetic model organisms [99–101] and NEBNext® reagents for Illumina® (New England Biolabs, Ipswich, MA). Samples were barcoded, multiplexed, and sequenced (single-end, 109bp) in a single lane on the Illumina HiSeq2000 platform at Tufts TUCF Genomics (Boston, MA).
RNA Sequencing on the Illumina HiSeq2000 platform yielded 145.3 million total reads, averaging 14.5 million reads per sample. Reads were trimmed and quality controlled to a Phred score of Q = 30. Read contamination was resolved prior to assembly by aligning all raw reads, using Bowtie (version 0.12.7) and custom perl scripts, against several indices and retaining only unmapped reads. These indices were constructed from the following inputs: (1) metazoan rRNA databases (NCBI), (2) viral, fungal, and bacterial genomes (NCBI), and (3) Illumina TruSeq adaptor sequences and PCR primers. Next, de novo assembly of the transcriptome was conducted in Trinity (version 8-14-2013), allowing for the detection of punitive alternative splice variants [102, 103]. Subsequently, contigs from our transcriptome were BLAST annotated against NCBI, UniProtKB’s Swiss-Prot, and TrEMBLE protein databases; matches with an e-value below 10−4 were considered protein-coding genes. Reads were aligned using Bowtie and include all partial and multiple alignments. Bowtie alignments of our dataset against selected genes from major signaling pathways as well as housekeeping genes, revealed high efficiency in gene recovery compared to available genomes of closely related species. 110.7 million reads mapped to our transcriptome and were used in gene expression analysis. Of these mapped reads, 66 % mapped to annotated transcripts. Blast output was parsed to the top hit for each contig, and NCBI gi numbers were converted to Entrez Gene IDs using ID mapping files from UniProt Knowledgebase (UniProtKB). All gene ID numbers provided are Entrez Gene IDs unless otherwise specified. Moreover, Entrez Gene IDs were mapped to gene ontology (GO) annotations using NCBI gene2go.
Differences in gene expression for all transcripts were analyzed from raw read counts using the R package DESeq2 1.4.5  and merged with annotation files (for breakdown of analyses, see Additional file 1: Table S1). Results from differential expression analysis were further analyzed for difference in expression of gene ontologies using the R package GOseq (version 1.16.2), which determines expression of gene ontology categories accounting for over-detection of differential expression for long or highly expressed transcripts . As multiple contigs often mapped to the same protein annotation, enrichment analysis was conducted on contigs with greatest read coverage for each given Entrez Gene ID. GOseq was run using gene annotation length as bias data and our GO category mapping file. P-values for overrepresented GO categories were adjusted using the Benjamini and Hochberg (1995) method for false discovery rate control. GO enrichment was visualized in REVIGO using GOseq output .
Homarus americanus is not an endangered or protected species, and ethical approval was not required for these experiments. Experimental procedures were conducted according best practices in the Homarus americanus community with efforts to minimize discomfort or distress.
Availability of supporting data
The reference transcriptome sequences are available on BioProject [accession number PRJNA297570]. Additionally, the Homarus americanus transcriptome, annotations, and raw read count data (doi:10.5061/dryad.h617h) are available on Dryad.
Central pattern generator
Neuronal nitric oxide synthase
- TRP channel:
Transient receptor potential channel
G-protein coupled receptor
- IRK channel:
Inward rectifying potassium channel
Stein PSG. Motor Systems, with specific references to the control of locomotion. Ann Rev Neurosci. 1978;1:61–81.
Kennedy D, Davis WJ. Organization of invertebrate motor systems. Comprehensive Physiology. 1977.
Grillner S, Wallen P. Central pattern generators for locomotion, with special reference to vertebrates. Annu Rev Neurosci. 1985;8(1):233–61.
Grillner S, Georgopoulos A, Jordan L, Stein P, Grillner S. Neurons, networks, and motor behavior. In.: Citeseer; Cambridge, Massachusetts: MIT Press. 1997.
Cooke IM. Studies on the crustacean cardiac ganglion. Comp Biochem Physiol C Toxicol Pharmacol. 1988;91(1):205–18.
Worden MK, Clark CM, Conaway M, Qadri SA. Temperature dependence of cardiac performance in the lobster Homarus americanus. J Exp Biol. 2006;209(6):1024–34.
Camacho J, Qadri SA, Wang H, Worden MK. Temperature acclimation alters cardiac performance in the lobster Homarus americanus. J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2006;192(12):1327–34.
Jury S, Watson W. Thermosensitivity of the lobster, Homarus americanus, as determined by cardiac assay. Biol Bull. 2000;199(3):257–64.
Wilkens JL, Kuramoto T, McMahon BR. The effects of six pericardial hormones and hypoxia on the semi-isolated heart and sternal arterial valve of the lobster Homarus americanus. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1996;114(1):57–65.
Maynard D, Welsh J. Neurohormones of the pericardial organs of brachyuran Crustacea. J Physiol. 1959;149(2):215–27.
Cooke IMS, R.E. Hormones and neurosecretion. In: Bliss D, editor. The Biology of Crustacea. vol. 3. New York: Academic Press; 1982: 205–290.
Christie AE. Crustacean neuroendocrine systems and their signaling agents. Cell Tissue Res. 2011;345(1):41–67.
Christie AE, Skiebe P, Marder E. Matrix of neuromodulators in neurosecretory structures of the crab Cancer borealis. J Exp Biol. 1995;198(12):2431–9.
Mulloney B, Acevedo LD, Chrachri A, Hall WM, Sherff CM. A confederation of neural circuits: control of swimmeret movements by a modular system of pattern generators. In: Frontiers in Crustacean Neurobiology, Birkhauser, Basel. Springer; 1990:439–47.
Murchison D, Chrachri A, Mulloney B. A separate local pattern-generating circuit controls the movements of each swimmeret in crayfish. J Neurophysiol. 1993;70(6):2620–31.
Krasne FB, Heitler WJ, Edwards DH. The Escape Behavior of a Crayfish. Derby, Charles, and Martin Thiel, eds. Crustacean Nervous Systems and Their Control of Behavior. OxfordUniversity Press, New York. 2014:396.
Davis W. The neural control of swimmeret beating in the lobster. J Exp Biol. 1969;50(1):99–117.
Davis WJ, Kennedy D. Command interneurons controlling swimmeret movements in the lobster: I. Types of effects on motoneurons. J Neurophysiol. 1972;35(1):1–12.
Smarandache-Wellmann C, Weller C, Wright TM, Mulloney B. Five types of nonspiking interneurons in local pattern-generating circuits of the crayfish swimmeret system. J Neurophysiol. 2013;110(2):344–57.
Zhang C, Guy RD, Mulloney B, Zhang Q, Lewis TJ. Neural mechanism of optimal limb coordination in crustacean swimming. Proc Nat Acad Sci. 2014;111(38):13840–5.
Kovac M. Abdominal movements during backward walking in crayfish. J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 1974;95(1):61–78.
Davis WJ. Lobster Righting Responses and their Neural Control. Proc R Soc Lond B. 1968;170(1021):435–56.
Mulloney B, Smarandache-Wellmann C. Neurobiology of the crustacean swimmeret system. Prog Neurobiol. 2012;96(2):242–67.
Atwood HL. An attempt to account for the diversity of crustacean muscles. American Zoologist. 1973;13(2):357–78.
Atwood HL. Organization and synaptic physiology of crustacean neuromuscular systems. Prog Neurobiol. 1976;7:291–391.
Davis W. Quantitative analysis of swimmeret beating in the lobster. J Exp Biol. 1968;48(3):643–62.
Qadri SA, Camacho J, Wang H, Taylor JR, Grosell M, Worden MK. Temperature and acid–base balance in the American lobster Homarus americanus. J Exp Biol. 2007;210(7):1245–54.
Marder E, Haddad S, Goeritz M, Rosenbaum P, Kispersky T. How can motor systems retain performance over a wide temperature range? Lessons from the crustacean stomatogastric nervous system. J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2015;201(9):851–6.
Marder E, Bucher D. Understanding circuit dynamics using the stomatogastric nervous system of lobsters and crabs. Annu Rev Physiol. 2007;69:291–316.
Marder E, Bucher D, Schulz DJ, Taylor AL. Invertebrate central pattern generation moves along. Curr Biol. 2005;15(17):R685–99.
Marder E, Bucher D. Central pattern generators and the control of rhythmic movements. Curr Biol. 2001;11(23):R986.
Chen R, Jiang X, Prieto Conaway MC, Mohtashemi I, Hui L, Viner R, et al. Mass spectral analysis of neuropeptide expression and distribution in the nervous system of the lobster Homarus americanus. J Proteome Res. 2010;9(2):818–32.
Hobert O. Regulatory logic of neuronal diversity: terminal selector genes and selector motifs. Proc Nat Acad Sci. 2008;105(51):20067–71.
Schulz DJ, Goaillard JM, Marder EE. Quantitative expression profiling of identified neurons reveals cell-specific constraints on highly variable levels of gene expression. Proc Nat Acad Sci. 2007;104(32):13187–91.
Temporal S, Lett KM, Schulz DJ. Activity-Dependent Feedback Regulates Correlated Ion Channel mRNA Levels in Single Identified Motor Neurons. Curr Biol. 2014;24(16):1899–904.
Tobin A-E, Cruz-Bermúdez ND, Marder E, Schulz DJ. Correlations in ion channel mRNA in rhythmically active neurons. PloS one. 2009;4(8), e6742.
Schulz DJ, Goaillard J-M, Marder E. Variable channel expression in identified single and electrically coupled neurons in different animals. Nat Neurosci. 2006;9(3):356–62.
Shruti S, Schulz DJ, Lett KM, Marder E. Electrical coupling and innexin expression in the stomatogastric ganglion of the crab Cancer borealis. J Neurophysiol. 2014;112(11):2946–58. doi:10.1152/jn.00536.2014.
Temporal S, Desai M, Khorkova O, Varghese G, Dai A, Schulz DJ, et al. Neuromodulation independently determines correlated channel expression and conductance levels in motor neurons of the stomatogastric ganglion. J Neurophysiol. 2011;107(2):718–27. doi:10.1152/jn.00622.2011.
O'Leary T, Williams AH, Caplan JS, Marder E. Correlations in ion channel expression emerge from homeostatic tuning rules. Proc Nat Acad Sci. 2013;110(28):E2645–54.
Hobert O, Carrera I, Stefanakis N. The molecular and gene regulatory signature of a neuron. Trends Neurosci. 2010;33(10):435–45.
Orchard I, Belanger JH, Lange AB. Proctolin: a review with emphasis on insects. J Neurobiol. 1989;20(5):470–96.
Miller MW, Sullivan RE. Some effects of proctolin on the cardiac ganglion of the Maine lobster, Homarus americanus (Milne Edwards). J Neurobiol. 1981;12(6):629–39.
Watson W, Augustine G, Benson J, Sullivan R. Proctolin and an endogenous proctolin-like peptide enhance the contractility of the Limulus heart. J Exp Biol. 1983;103(1):55–73.
Mulloney B, Acevedo LD, Bradbury AG. Modulation of the crayfish swimmeret rhythm by octopamine and the neuropeptide proctolin. J Neurophysiol. 1987;58(3):584–97.
Hooper SL, Marder E. Modulation of the lobster pyloric rhythm by the peptide proctolin. J Neurosci. 1987;7(7):2097–112.
Heinzel H-G. Gastric mill activity in the lobster. II. Proctolin and octopamine initiate and modulate chewing. J Neurophysiol. 1988;59(2):551–65.
Heinzel H-G, Selverston AI. Gastric mill activity in the lobster. III. Effects of proctolin on the isolated central pattern generator. J Neurophysiol. 1988;59(2):566–85.
Schwarz TL, Lee GM-h, Siwicki KK, Standaert DG, Kravitz EA. Proctolin in the lobster: the distribution, release, and chemical characterization of a likely neurohormone. J Neurosci. 1984;4(5):1300–11.
Stangier J, Dircksen H, Keller R. Identification and immunocytochemical localization of proctolin in pericardial organs of the shore crab. Carcinus Maenas Peptides. 1986;7(1):67–72.
Beale KM, Towle DW, Jayasundara N, Smith CM, Shields JD, Small HJ, et al. Anti-lipopolysaccharide factors in the American lobster Homarus americanus: Molecular characterization and transcriptional response to Vibrio fluvialis challenge. Comp Biochem Physiol Part D Genomics Proteomics. 2008;3(4):263–9.
Lin M-C, Pan C-Y, Hui C-F, Chen J-Y, Wu J-L. Shrimp anti-lipopolysaccharide factor (SALF), an antimicrobial peptide, inhibits proinflammatory cytokine expressions through the MAPK and NF-κB pathways in LPS-induced HeLa cells. Peptides. 2013;40:42–8.
Liu Y, Cui Z, Luan W, Song C, Nie Q, Wang S, et al. Three isoforms of anti-lipopolysaccharide factor identified from eyestalk cDNA library of swimming crab Portunus trituberculatus. Fish Shellfish Immunol. 2011;30(2):583–91.
Adamo SA. Modulating the modulators: parasites, neuromodulators and host behavioral change. Brain Behav Evol. 2002;60(6):370–7.
Baines D, Downer RGH. Octopamine enhances phagocytosis in cockroach hemocytes: Involvement of inositol trisphosphate. Arch Insect Biochem Physiol. 1994;26(4):249–61.
Baines D, DeSantis T, Downer RG. Octopamine and 5-hydroxytryptamine enhance the phagocytic and nodule formation activities of cockroach (Periplaneta americana) haemocytes. J Insect Physiol. 1992;38(11):905–14.
Kim GS, Kim Y. Up-regulation of circulating hemocyte population in response to bacterial challenge is mediated by octopamine and 5-hydroxytryptamine via Rac1 signal in Spodoptera exigua. J Insect Physiol. 2010;56(6):559–66.
Diehl-Jones WL, Mandato CA, Whent G, Downer RGH. Monoaminergic regulation of hemocyte activity. J Insect Physiol. 1996;42(1):13–9.
Johnson EC, Garczynski SF, Park D, Crim JW, Nässel DR, Taghert PH. Identification and characterization of a G protein-coupled receptor for the neuropeptide proctolin in Drosophila melanogaster. Proc Nat Acad Sci. 2003;100(10):6198–203.
H-p L, R-y C, Q-x Z, Peng H, Wang K-j. Differential gene expression profile from haematopoietic tissue stem cells of red claw crayfish, Cherax quadricarinatus, in response to WSSV infection. Dev Comp Immunol. 2011;35(7):716–24.
Marder E. Neuromodulation of neuronal circuits: back to the future. Neuron. 2012;76(1):1–11.
Kyriakatos A, Molinari M, Mahmood R, Grillner S, Sillar KT, El Manira A. Nitric oxide potentiation of locomotor activity in the spinal cord of the lamprey. J Neurosci. 2009;29(42):13283–91.
Song J, Kyriakatos A, El Manira A. Gating the polarity of endocannabinoid-mediated synaptic plasticity by nitric oxide in the spinal locomotor network. J Neurosci. 2012;32(15):5097–105.
El Manira A. Dynamics and plasticity of spinal locomotor circuits. Curr Opin Neurobiol. 2014;29:133–41.
Mita A, Yoshida M, Nagayama T. Nitric oxide modulates a swimmeret beating rhythm in the crayfish. J Exp Biol. 2014;217(24):4423–31.
Maqueira B, Chatwin H, Evans PD. Identification and characterization of a novel family of Drosophilaβ‐adrenergic‐like octopamine G‐protein coupled receptors. J Neurochem. 2005;94(2):547–60.
Evans PD, Maqueira B. Insect octopamine receptors: a new classification scheme based on studies of cloned Drosophila G-protein coupled receptors. Invert Neurosci. 2005;5(3–4):111–8.
Doitsidou M, Flames N, Topalidou I, Abe N, Felton T, Remesal L, et al. A combinatorial regulatory signature controls terminal differentiation of the dopaminergic nervous system in C. elegans. Genes Dev. 2013;27(12):1391–405.
Flames N, Hobert O. Gene regulatory logic of dopamine neuron differentiation. Nature. 2009;458(7240):885–9.
Hobert O. Gene regulation by transcription factors and microRNAs. Science. 2008;319(5871):1785–6.
Serrano-Saiz E, Poole RJ, Felton T, Zhang F, De La Cruz ED, Hobert O. Modular control of glutamatergic neuronal identity in C. elegans by distinct homeodomain proteins. Cell. 2013;155(3):659–73.
Tursun B, Patel T, Kratsios P, Hobert O. Direct conversion of C. elegans germ cells into specific neuron types. Science. 2011;331(6015):304–8.
Dougherty KJ, Zagoraiou L, Satoh D, Rozani I, Doobar S, Arber S, et al. Locomotor rhythm generation linked to the output of spinal shox2 excitatory interneurons. Neuron. 2013;80(4):920–33.
Stangier J, Hilbich C, Beyreuther K, Keller R. Unusual cardioactive peptide (CCAP) from pericardial organs of the shore crab Carcinus maenas. Proc Nat Acad Sci. 1987;84(2):575–9.
Atkinson NS, Robertson GA, Ganetzky B. A component of calcium-activated potassium channels encoded by the Drosophila slo locus. Science. 1991;253(5019):551–5.
Elkins T, Ganetzky B, Wu C-F. A Drosophila mutation that eliminates a calcium-dependent potassium current. Proc Nat Acad Sci. 1986;83(21):8415–9.
Atkinson NS, Brenner R, Chang W-m, Wilbur J, Larimer JL, Yu J. Molecular separation of two behavioral phenotypes by a mutation affecting the promoters of a Ca-activated K channel. J Neurosci. 2000;20(8):2988–93.
McKiernan EC. Effects of manipulating slowpoke calcium-dependent potassium channel expression on rhythmic locomotor activity in Drosophila larvae. PeerJ. 2013;1, e57.
Iyengar A, Wu C-F. Flight and seizure motor patterns in Drosophila mutants: Simultaneous acoustic and electrophysiological recordings of wing beats and flight muscle activity. J Neurogenet. 2014;28(3–4):316–28.
Koyama H, Morishige K-I, Takahashi N, Zanelli JS, Fass DN, Kurachi Y. Molecular cloning, functional expression and localization of a novel inward rectifier potassium channel in the rat brain. FEBS Lett. 1994;341(2):303–7.
Vandenberg CA. Inward rectification of a potassium channel in cardiac ventricular cells depends on internal magnesium ions. Proc Nat Acad Sci. 1987;84(8):2560–4.
Tang LS, Taylor AL, Rinberg A, Marder E. Robustness of a Rhythmic Circuit to Short-and Long-Term Temperature Changes. J Neurosci. 2012;32(29):10075–85.
Rinberg A, Taylor AL, Marder E. The Effects of Temperature on the Stability of a Neuronal Oscillator. PLoS Comput Biol. 2013;9(1), e1002857.
Tang LS, Goeritz ML, Caplan JS, Taylor AL, Fisek M, Marder E. Precise temperature compensation of phase in a rhythmic motor pattern. PLoS Biol. 2010;8(8), e1000469.
Lewis L, Ayers J. Temperature preference and acclimation in the Jonah Crab, Cancer borealis. J Exp Mar Bio Ecol. 2014;455:7–13.
Crossin G, Al-Ayoub S, Jury S, Howell W. Behavioral thermoregulation in the American lobster Homarus americanus. J Exp Biol. 1998;201(3):365–74.
Jury SH, Watson WH. Seasonal and sexual differences in the thermal preferences and movements of American lobsters. Can J Fish Aquat Sci. 2013;70(11):1650–7.
Clapham DE, Runnels LW, Strübing C. The TRP ion channel family. Nat Rev Neurosci. 2001;2(6):387–96.
Guimaraes MZP, Jordt SE. TRPA1: A Sensory Channel of Many Talents. In: Liedtke WB, Heller S, editors. TRP Ion Channel Function in Sensory Transduction and Cellular Signaling Cascades. Boca Raton (FL): CRC Press/Taylor & Francis; 2007. Chapter 11. Available from: http://www.ncbi.nlm.nih.gov/books/NBK5237/.
Peier AM, Moqrich A, Hergarden AC, Reeve AJ, Andersson DA, Story GM, et al. A TRP channel that senses cold stimuli and menthol. Cell. 2002;108(5):705–15.
Rosenzweig M, Kang K, Garrity PA. Distinct TRP channels are required for warm and cool avoidance in Drosophila melanogaster. Proc Nat Acad Sci. 2008;105(38):14668–73.
Sokabe T, Tominaga M. A temperature-sensitive TRP ion channel, Painless, functions as a noxious heat sensor in fruit flies. Commun Integr Biol. 2009;2(2):170–3.
Mykles DL. The mechanism of fluid absorption at ecdysis in the American lobster Homarus americanus. J Exp Biol. 1980;84(1):89–102.
Sher E, Rosa P, Francolini M, Codignola A, Morlacchi E, Taverna E, et al. Metabolism and trafficking of N-type voltage-operated calcium channels in neurosecretory cells. J Bioenerg Biomembr. 1998;30(4):399–407.
Passafaro M, Clementi F, Sher E. Metabolism of omega-conotoxin-sensitive voltage-operated calcium channels in human neuroblastoma cells: modulation by cell differentiation and anti-channel antibodies. J Neurosci. 1992;12(9):3372–9.
Levitan ES, Takimoto K. Dynamic regulation of K+ channel gene expression in differentiated cells. J Neurobiol. 1998;37(1):60–8.
Takimoto K, Fomina AF, Gealy R, Trimmer JS, Levitan ES. Dexamethasone rapidly induces Kv1.5 K+ channel gene transcription and expression in clonal pituitary cells. Neuron. 1993;11(2):359–69.
Marder E, Hooper SL, Siwicki KK. Modulatory action and distribution of the neuropeptide proctolin in the crustacean stomatogastric nervous system. J Comp Neurol. 1986;243(4):454–67.
Schunter C, Vollmer SV, Macpherson E, Pascual M. Transcriptome analyses and differential gene expression in a non-model fish species with alternative mating tactics. BMC genomics. 2014;15(1):167.
Libro S, Kaluziak ST, Vollmer SV. RNA-seq Profiles of Immune Related Genes in the Staghorn Coral Acropora cervicornis Infected with White Band Disease. PloS one. 2013;8(11), e81821.
Hemond EM, Kaluziak ST, Vollmer SV. The genetics of colony form and function in Caribbean Acropora corals. BMC Genomics. 2014;15(1):1133.
Haas BJ, Papanicolaou A, Yassour M, Grabherr M, Blood PD, Bowden J, et al. De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis. Nat Protoc. 2013;8(8):1494–512.
Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, et al. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol. 2011;29(7):644–52.
Anders S, Huber W. Differential expression analysis for sequence count data. Genome Biol. 2010;11:R106.
Young MD, Wakefield MJ, Smyth GK, Oshlack A. Gene ontology analysis for RNA-seq: accounting for selection bias. Genome Biol. 2010;11:R14.
Supek F, Bošnjak M, Škunca N, Šmuc T. REVIGO summarizes and visualizes long lists of gene ontology terms. PloS One. 2011;6(7), e21800.
The authors would like to thank A. Selverston for helpful feedback and insight, and S. Libro and E. Hemond for additional guidance.
This work was supported by Schlumberger, and NSF Grant 0963010 as part of the Academic Research Infrastructure Recovery and Reinvestment Program.
The authors declare that they have no competing interests.
LM, JA, and SV designed the experiments and edited manuscript. LM performed molecular work. SK performed QC and read alignment. LM performed data analysis and wrote manuscript. All authors read and approved manuscript.
Experimental design for represented differential gene expression analyses conducted in DESeq2. Table S2. Terminal selector genes differentially expressed in heart tissue compared to muscle tissue and central nervous system tissue (supraesophogeal ganglion, abdominal ganglion) compared to muscle tissue. Count indicates the total number of transcripts annotating to proteins (e-val < 10−4) identified as transcription factors, co-factors, or other regulatory genes involved in differentiating the terminal identity of a neuron type. Columns denote the total number of DE transcripts (DE, p < 0.05), and number of upregulated (up) and downregulated (down) DE transcripts in contrasts: heart vs. muscle tissue and nervous system vs. muscle tissue. Table S3. Gene ontology analysis of abdominal nerve cord vs. heart tissue. Analysis includes only significantly enriched GO terms (adj p value < 0.05) and depicts the ‘count’ or number contigs mapping to genes in the GO category, number of DE contigs (adj p < 0.05), DE contigs upregulated (“up”, log2foldchange > 0 or downregulated (“down”, log2foldchange < 0) in the abdominal ganglia compared to the heart ganglia. Table S4. Differentially expressed membrane channels in the abdominal ganglia compared to the heart. Table includes only DE contigs with a log2fold changes greater than four or less than negative four. (DOCX 111 kb)
Heatmap display of sample-to-sample Euclidean distances between samples, calculated by regularized log transformation. (TIFF 1415 kb)
Treemap of enriched biological process GO categories in heart tissues compared to muscle tissues. Box size indicates relative level of overrepresentation in heart tissues. The only significantly overrepresented biological process was response to heat (GO: 0009408), with over 50 % of involved genes differentially expressed (18 of 32 genes in category, p < 0.05). (JPG 1509 kb)
About this article
- Gene expression
- Nervous system
- Central pattern generation
- Neuronal differentiation
- Homarus americanus