- Research article
- Open Access
Gene silencing pathways found in the green alga Volvox carteri reveal insights into evolution and origins of small RNA systems in plants
BMC Genomicsvolume 17, Article number: 853 (2016)
Volvox carteri (V. carteri) is a multicellular green alga used as model system for the evolution of multicellularity. So far, the contribution of small RNA pathways to these phenomena is not understood. Thus, we have sequenced V. carteri Argonaute 3 (VcAGO3)-associated small RNAs from different developmental stages.
Using this functional approach, we define the Volvox microRNA (miRNA) repertoire and show that miRNAs are not conserved in the closely related unicellular alga Chlamydomonas reinhardtii. Furthermore, we find that miRNAs are differentially expressed during different life stages of V. carteri. In addition to miRNAs, transposon-associated small RNAs or phased siRNA loci, which are common in higher land plants, are highly abundant in Volvox as well. Transposons not only give rise to miRNAs and other small RNAs, they are also targets of small RNAs.
Our analyses reveal a surprisingly complex small RNA network in Volvox as elaborate as in higher land plants. At least the identified VcAGO3-associated miRNAs are not conserved in C. reinhardtii suggesting fast evolution of small RNA systems. Thus, distinct small RNAs may contribute to multicellularity and also division of labor in reproductive and somatic cells.
Volvocine algae are a sub-group of green algae ranging from unicellular species such as Chlamydomonas reinhardtii (C. reinhardtii) to the multicellular genus Volvox. V. carteri is a freshwater alga with female and male cells that can reproduce both sexually and asexually and is composed of two different cell types . Asexual forms are characterized by a spheroid that is composed of approx. 2000 somatic cells and encloses several reproductive cells. Each of these so called gonidia can undergo symmetric and asymmetric cell divisions to form a new colony of somatic cells and gonidia . The sexual life cycle is started by a sex-inducing pheromone that can be secreted by male strains and is functional at very low concentrations . Pheromone signaling induces the sexual differentiation program and therefore the generation of egg cells in female strains, while male strains form sperm packages that are subsequently released into the water . Dimorphic sexes have evolved several times in plants, however their origins are unclear. The fact, that the sexually dimorphic and multicellular species V. carteri and the unicellular species C. reinhardtii had a common ancestor about 200 million years ago  makes Volvox an ideal system for studying the evolution of these processes .
Non-coding RNAs, including small RNAs, have been associated with processes that are crucial to eukaryotic evolution. Small RNAs are processed from longer precursor molecules to their mature forms that are subsequently bound by a member of the Argonaute (AGO) protein family . Argonaute proteins are characterized by distinct domains that anchor both the 5′ and the 3′ end of the small RNA . Some but not all Argonaute proteins are endonucleases and can cleave target RNAs that are complementary to the bound small RNAs [8, 9]. In many organisms, several Argonaute protein genes exist and especially in plants each AGO protein prefers and selects different small RNAs . In land plants, several different pathways that make use of specialized small RNAs are known [10, 11]. For example, short interfering RNAs (siRNAs) are processed from transgene- or virus-derived double stranded (ds) RNAs and guide sequence-specific cleavage of complementary (foreign) RNAs. Furthermore, small RNAs can direct DNA methylation and subsequent gene silencing processes in a pathway known as RNA-dependent DNA methylation (RdDM). MicroRNAs (miRNAs) are processed from endogenous miRNA genes and guide repression of complementary target mRNA expression as a means of gene regulation. Unlike in animals, where miRNAs bind to partially complementary sequences, plant miRNAs mainly repress their target mRNAs by hybridizing to fully complementary sites followed by sequence-specific cleavage. So-called trans acting siRNAs (tasiRNAs) are plant-specific and also regulate gene expression. This pathway is initiated by a miRNA (most prominently miR-390), which guides cleavage of a target. The cleaved transcript is used as template by RNA-dependent RNA polymerases (RdRPs) to generate a long dsRNA, which is cleaved by DCL4 to produce many tasiRNAs thus amplifying the signal. These RNAs, in turn, silence the respective locus by transcript cleavage . Recently, it has been shown in A. thaliana that a miRNA can also guide cleavage of transposon transcripts and, similarly, the cleaved transcript serves as template for dsRNA, which gives subsequently rise to siRNAs that repress transposon expression . SiRNAs and miRNAs have been identified in C. reinhardtii, indicating that small RNA pathways have evolved at early stages of plant evolution [14, 15]. Given the importance of different small RNAs in processes closely associated with multicellular development in land plants, we speculated that they might have played a role in the transition from uni- to multicellularity in green algae. MiRNA candidates have been reported in V. carteri, but their function remains unknown .
Here, we have performed small RNA-Seq as well as RNA-Seq of mRNAs from different stages of V. carteri providing a global and comprehensive atlas of gene expression in somatic and reproductive cells. To identify functional small RNAs, we isolated VcAGO3 protein and cloned and deep sequenced associated small RNAs. Using this functional approach, we clearly define miRNAs as well as their specific expression under different conditions. Of note, the identified miRNAs are not conserved in C. reinhardtii. Furthermore, we find miRNAs as well as siRNAs originating from transposons such as Jordan or Kangaroo, for example. Strikingly, miRNAs are not only expressed from transposons but also seem to target such elements, suggesting miRNA-guided repression of transposable elements. Finally, we also provide evidence for loci that generate phased siRNAs similar to tasiRNAs found in A. thaliana. Although green algae and higher land plants including A. thaliana diverged very early and evolution proceeded independently, we provide evidence that many small RNA pathways found in land plants have also evolved in V. carteri.
Transcriptome sequencing and annotation
We sought to characterize expression activity and dynamics not only of small RNAs, but also of mRNAs in V. carteri, since these are often modulated by small RNAs. When we started this study, only very little transcriptomic data had been available for V. carteri in general. We therefore cloned and sequenced RNAs under various experimental conditions (Additional file 1: Table S1). We included somatic cells and gonidia under vegetative growth conditions, somatic cells as well as gonidia after induction of sexual reproduction, along with somatic and egg cells from a female culture (fully differentiated). Using all single-end as well as paired-end sequencing data, a transcriptome assembly was generated in order to analyze mRNA transcripts in more detail. This comprehensive data set enabled us to analyze transcripts and their possible connection to small RNAs in better detail. All RNAseq data are available at the NCBI SRA under PRJNA266874 for further analysis.
AGO-associated small RNAs of V. carteri
To identify functional small RNA pathways in V. carteri, we first examined available transcriptome data and our own RNA-Seq data for the presence of potential factors that are known to be involved in RNA silencing pathways and that are also known to be conserved proteins. We found a PAZ/PIWI domain-containing gene, typical for Argonaute genes, which we refer to as VcAGO3, since a similar transcript had already been annotated at this locus (XM_002952848) (Fig. 1a). Furthermore, we found genes for an additional AGO protein (although we could not amplify the complete transcript from cDNA for this gene so far), one Dicer, one RNA-dependent RNA Polymerase (RdRP) and a HEN1 homolog (Additional file 2: Figure S1), suggesting that several small RNA pathways might operate in V. carteri .
To identify functional small RNAs, we expressed myc-tagged VcAGO3 in V. carteri by gold particle-mediated plasmid delivery, isolated associated small RNAs by anti-myc immunoprecipitation and cloned and sequenced them (Fig. 1b). The length of the VcAGO3-associated RNAs peaks at 21 and 22 nt (Fig. 1c). While the GC content of the genome of V. carteri is about 60 %, the 5′ terminal nucleotide of the bound small RNAs is mainly uridine, suggesting that the VcAGO3 MID domain accommodates preferentially 5′ terminal uridines, similar to human Ago2 and Arabidopsis Ago1 [17, 18] (Fig. 1d). VcAGO3-associated small RNAs that map to the genome can be grouped into different functional categories including repetitive elements (RE), protein coding regions (sense and antisense), phased siRNAs or miRNAs (Fig. 1e and f). Since the transcriptome and RNA classes in general are only poorly annotated in V. carteri, the identity of the largest part of our library cannot be deduced yet. However, these sequences are bound by VcAGO3 and therefore are presumably functional (see also below and Fig. 4).
To predict miRNAs in Volvox, we utilized the novel miRNA identification tool miRA  using criteria adopted from the prediction of miRNAs in C. reinhardtii . In total, 490 miRNAs were predicted when running miRA on the VcAGO3 data set as well as six libraries from different cells and life stages from V. carteri (Additional file 3: Table S2 and Additional file 4: Figure S2). These predicted miRNAs fall into 324 miRNA families (miRNAs with the same sequence, but different genomic location), the largest one comprising 48 members and 67 families had two or more members. The nucleotide preference for VcAGO3-bound miRNAs is even more pronounced towards a U at the 5′ end (Fig. 1g), indicating high specificity of our miRNA identification approach. In conclusion, we identified VcAGO3-associated potential miRNAs in different cell types and under different cellular conditions.
Experimental validation of V. carteri miRNAs
A general feature of small RNAs or miRNAs in particular in plants is a 2′O-methylation at the 3′ end . To test whether our miRNA candidates contain such characteristic modifications, we performed β-elimination experiments with total RNA from somatic cells and reproductive cells (gonidia). In this chemical reaction, an unmethylated 3′ end will lose its terminal base and as a consequence, migrate faster in polyacrylamide gels. As a positive control, an unrelated RNA oligonucleotide with unprotected 3′ end was spiked in prior to the reaction. While the positive control clearly shifted after treatment (Fig. 2a), all analyzed small RNAs from V. carteri migrated at the same length as before indicating that they are indeed modified at their 3’ ends (Fig. 2a). These findings are consistent with the presence of a HEN1 homolog in the V. carteri genome (Additional file 2: Figure S1) and suggest that our candidate RNAs are most likely miRNA.
After identifying miRNA sequences in V. carteri and establishing that they are modified at their 3′ end, we investigated miRNA expression under varying physiological conditions (Fig. 2b). We harvested samples at different life stages of V. carteri (asexual growth (V), 16 h induction of the sexual program (I) and female sexual differentiation (F)) and the culture from each stage was split into the two different cell types of V. carteri (somatic cells and reproductive cells). RNA blots performed with these samples show that miRNAs are indeed regulated between cell types and some are even regulated during development (Fig. 2b) indicating that V. carteri miRNAs might play an important role not only for cellular maintenance but also during differentiation processes. Of note, the U6 loading control signal is much weaker for the reproductive cells and therefore, only limited comparisons between somatic and reproductive cells can be made.
In plants, functional small RNAs are incorporated into AGO proteins and guide them to complementary target RNAs for cleavage. The VcAGO3 protein has a putative catalytic triad composed of the amino acids DDD. Although the canonical motif for a cleavage-competent Ago protein is DDH, AtAgo2 contains DDD as well and appears to be a catalytically active enzyme . Cleavage activity of AGO proteins is usually necessary for gene silencing in plants, although mechanisms similar to mammalian AGO proteins have also been proposed . In order to test the cleavage activity of AGO proteins, in vitro cleavage assays can be performed in which AGO proteins are purified from cell lysates and incubated with radioactively 5′ end-labeled, artificial targets that are designed to be fully complementary to an endogenous small RNA bound by the respective AGO protein. After the catalytic reaction, the target RNA is cleaved into two pieces with only the 5′ end being detectable due to its radiolabeled 5′ end. In order to test VcAGO3 for catalytic activity, V. carteri cultures were transformed with a plasmid carrying either myc-VcAGO3 or myc-CrGFP (control, GFP codon-optimized for C. reinhardtii). After a short selection period, the cultures were harvested, lysed and an immunoprecipitation (IP) was performed using anti-myc antibodies. Each IP was split into three reactions and cleavage assays were performed using three different small RNA target sequences (Fig. 2c). None of the VcAGO3 reactions generated specific cleavage products (Fig. 2c, lanes 3, 6 and 9), while a Flag-tagged AtAGO1 (used as a positive control) showed clear and strong cleavage activity (Fig. 2c, lanes 11 and 13). All proteins were expressed and precipitated successfully as shown by protein blot (Fig. 2d). VcAGO3 appears to be inactive in standard in vitro cleavage assays. We cannot exclude, however, that VcAGO3 functions as slicer endonuclease under different in vivo conditions.
V. carteri small RNAs associated with transposable elements
To further understand the putative functions of VcAGO3-bound small RNAs, we analyzed their genomic origins in more detail. Interestingly, miR178al and miR357b originate from a transposon termed Jordan (Fig. 3a). Jordan is a highly abundant transposable element, which resembles the transposable elements En/Spm as well as members of the so-called “CACTA” family found also in higher plants . These elements transpose via a DNA intermediate and contain terminal inverted repeats (TIRs). In the case of Jordan, the TIRs cause a secondary structure that is similar to and recognized as a miRNA precursor and each of these elements gives rise to a miRNA (Fig. 3a). Of note, the precursors of the MIR178 and MIR357 families are highly similar to each other, with one family showing a strong expression of the 5′ arm miRNA sequence (MIR178 family), while the other expresses the 3′ arm (MIR357 family) more strongly. Thus, both miRNAs could target each other’s precursors with perfect complementarity (Additional file 5: Figure S3). To support the inferences from our small RNA-Seq experiments, we performed RNA blots using probes against the Jordan-encoded miRNAs (Fig. 3b), which readily validate our sequencing data.
Because transposon-derived miRNAs can trigger a wave of secondary siRNA species in A. thaliana , we searched for additional VcAGO3-associated small RNAs from Jordan. We found many small RNAs originating mainly from the 5′ 0.5 kb portion (Fig. 3c, upper panel). Whether these transposon-derived siRNAs depend on the Jordan-encoded miRNAs is currently unknown. Furthermore, we analyzed Jordan for potential miRNA target sites and found that several V. carteri miRNAs can target Jordan (Fig. 3a, black bars). Again, these target sites are largely located in the 5′ 0.5 kb portion of Jordan. Taken together, our data suggest that the transposon Jordan not only generates two miRNAs and a number of siRNAs, but might also be targeted by the VcAGO3-associated small RNA system, presumably to repress Jordan expression. Of note, VcAGO3 appears to be catalytically inactive in vitro (Fig. 2a). This might suggest that cleavage-independent silencing mechanisms (e.g. transcriptional silencing by heterochromatin formation) could be active in this process.
We next analyzed whether other transposons are similar sources of different classes of small RNAs. Close examination of VcAGO3-associated small RNAs did not reveal miRNA genes that are encoded by transposons and pass our annotation criteria. However, transposons such as Gypsy5-I (Fig. 3c, middle panel) or Gypsy4-I (bottom panel) produce a large number of VcAGO3-associated siRNAs. Furthermore, Gypsy5-I is in addition targeted by MIR414 and MIR254. Strikingly, a global analysis of our sequencing data revealed that most transposons give rise to small RNAs (Additional file 6: Figure S4), suggesting control of expression through small RNA-guided gene silencing pathways.
At least one miRNA, miR178al, appears to be expressed more strongly in reproductive cells (Fig. 3b), which might suggest that Jordan expression is generally lower in such cells. Indeed, our RNAseq data reveals that less Jordan-derived reads were cloned from reproductive cells compared to somatic cells. Generally, we observe, with some exceptions, a tendency of reduced transposon activity in reproductive cells (Additional file 7: Figure S5) suggesting repression of such elements in reproductive cells.
Other V. carteri small RNAs
In A. thaliana, tasiRNAs are produced from RNAs that are specifically cleaved by miRNAs such as miR390. Biogenesis involves dsRNA synthesis by RDR6 and cleavage by DCL4, which progressively cleaves the RNA from the ends and produces phased tasiRNAs . Since we find a putative RdRP in the V. carteri genome, we examined our VcAGO3-associated siRNAs for phased siRNA signatures. Indeed, we found several loci that produce phased siRNAs. Most prominently, phased siRNA loci can be found within the transposon Kangaroo (Fig. 4a). Whether these siRNAs require miRNA-guided cleavage as observed for tasiRNAs in A. thaliana, is unclear. Similar to other transposons, Kangaroo produces various other VcAGO3-associated small RNAs in addition to the phased siRNAs (Fig. 4a upper part and b). To further investigate phased siRNAs, we performed RNA blots (Fig. 4c). The two most abundant single phased siRNAs were readily detectable, with the highest expression in reproductive female cells.
A large portion of the VcAGO3-bound small RNAs did not fit into any known small RNA category, but could be aligned to the genome, indicating that they originated from V. carteri, and not from a potential associated microbe. In fact, about 11 % of the putative intergenic reads (7 % of total reads) came from eight specific loci (Fig. 4d and e), further supporting that such sequences are likely not due to promiscuous binding of VcAGO3 to RNA degradation products. The specificity is further supported by the observation that most of the small RNAs within a peak are identical. It is also unlikely that these reads are unspecifically amplified during library construction since RNA blots supported robust expression and stability (Fig. 4f). For expression analysis, we performed Northern blotting of total RNA obtained from somatic and reproductive cells. Probes directed against the most prominent scaffolds (Fig. 4d) are readily detectable thus suggesting that these reads are indeed abundant small RNAs (Fig. 4f). It is therefore tempting to speculate that these VcAGO3-associated reads might represent a novel class of small RNAs.
Comparative genomics of small RNAs in green algae
Our sequencing data demonstrate that V. carteri contains many miRNA candidates. It has been shown before that C. reinhardtii expresses miRNAs and other small RNA classes [14, 15]. Since both species are closely related, we analyzed conservation of our VcAGO3-associated miRNAs. For direct examination, total RNA from V. carteri as well as C. reinhardtii was extracted and several miRNAs were assayed with RNA blots (Fig. 4g). Indeed, V. carteri MIR322a is not detected in C. reinhardtii. Vice versa, a number of sRNAs were only found in C. reinhardtii but not in V. carteri samples. Subsequently, a global analysis of miRNA sequences was performed using multiple sequence alignments of every miRNA of V. carteri with every miRNA of C. reinhardtii  or the liverwort Pellia endiviifolia . Consistent with our RNA blot experiments, these global analyses revealed very little conservation between V. carteri and C. reinhardtii (Additional file 8: Figure S6A) or V. carteri and P. endiviifolia (Additional file 8: Figure S6B). Only three miRNAs had before been reported as potentially conserved between C. reinhardtii and P. endiviifolia .
We have cloned and sequenced VcAGO3-associated small RNAs from different V. carteri stages. Since we select for VcAGO3-bound small RNAs only, it is very likely that we identify functional small RNAs and minimize background cloning that would contaminate our libraries. Indeed, our VcAGO3 pull down approach allows for a clear definition of V. carteri miRNAs . Of note, our miRNAs are distinct from those reported recently . This might be due to different V. carteri strains that have been used or, more likely, to mis-annotation of miRNAs as a result of background sequencing from total RNA samples.
An intriguing observation from our small RNA profiles was that many VcAGO3-bound small RNAs originate from transposons. Different classes including miRNAs, siRNAs and even phased siRNAs have been identified in conjunction with transposons. Since miRNA genes are encoded by the transposon Jordan, it is conceivable that this mobile genetic element influences spreading of miRNA genes and might therefore contribute to evolution. Furthermore, transposons are at the same time targeted by miRNAs suggesting that miRNAs might repress the expression of such elements. In addition, transposons appear to produce large numbers of siRNAs especially in reproductive cells. It is therefore likely that transposon targeting by small RNAs might be involved in repressing transposable elements in reproductive cells, a phenomenon that is often observed in animals . Interestingly, computational analyses have suggested that both A. thaliana and Oryza sativa have transposable elements that encode simultaneously siRNAs and miRNAs . Whether this suggests a common origin, or convergent evolution of such mechanisms in algae and land plants is unclear.
Our analyses reveal also interesting evolutionary aspects when analyzing conservation of closely related species. At least the VcAGO3-associated miRNAs are not conserved in the closely related single cellular alga C. reinhardtii, suggesting evolution of different miRNAs presumably required for multicellularity and division of labor between somatic and reproductive cells. This is remarkable given that so many protein-coding genes are well conserved between the two species. It will be interesting to analyze whether other small RNAs, e.g. small RNAs that target transposable elements such as Jordan, are conserved. Our findings suggest that targeting such mobile genetic elements by the small RNA pathways developed very early during evolution and the last universal common ancestor of all eukaryotes used such strategies already.
We conclude from our analyses that VcAGO3 not only associates with miRNAs but also many other classes of functional small RNAs. These small RNAs are most likely not conserved in C. reinhardtii suggesting evolution of small RNA repertoires that are important for multicellularity and the division of labor. Interestingly, a distinct portion of the VcAGO3-associated small RNAs is derived from transposable elements. These include miRNAs, classical siRNAs and even phased siRNAs, which are common to higher land plants. Taken together, our data identify an extended small RNA system in V. carteri, which appears to be as complex as in higher plants.
Separation of cell types
About 5000 asexual growing V. carteri spheroids at the stage shortly before the onset of embryogenesis were collected by filtration on a 100 μm mesh nylon screen and broken by passing them through a 0.6 mm hypodermic needle. The suspension was filtered on a 100 μm mesh nylon screen, which allows free reproductive cells (gonidia), free somatic cells and small fragments of the somatic cell layer to pass but retaining larger fragments of somatic cell layers nearly free from gonidia. The filtrate was passed in a second step through a 40 μm mesh nylon screen which retained only gonidia and small fragments of the somatic cell layer. The resuspended residue was allowed to settle down repeatedly in a small volume separating the reproductive cells from somatic cell layers.
The separation of gonidia and somatic cell layers of sexual induced V. carteri spheroids at the stage shortly before the onset of embryogenesis and 16 h after application of the sex-inducing pheromone was done in the same manner as described above.
The separation of egg cells and somatic cells of sexual growing V. carteri spheroids 64 h after application of the sex-inducing pheromone was done in a modified procedure: Egg cells were set free by passing the spheroids twice through a 0.5 mm hypodermic needle. A first filtration step on a 40 μm mesh nylon screen retained large fragments of the somatic cell layer. Egg cells which pass through were collected on a 10 μm mesh nylon screen and purified further by let them settle down repeatedly in a small volume. The residue of the first filtration step was dissociated once more by passing through a 0.4 mm hypodermic needle. Fragments of the somatic cell layer were collected on a 40 μm mesh nylon screen.
Production of stable V. carteri strains using the gold particle gun
Precipitation of DNA (plasmids) onto gold-microcarriers
For each transformation, 10 μg of plasmid DNA encoding the selection marker (pPmr3, see ) and 10 μg plasmid encoding the target gene were used. The DNA solution was added to 0.5 μmol gold particles while mixing vigorously. Continuing the shaking, 125 μmol CaCl2 (50 μl of 2.5 M) and 2 μmol spermidin (20 μl of 100 mM) were added. The mixture was further incubated at 4 °C for 30 min under continuous shaking. To precipitate the DNA onto the gold, 200 μl of 100 % ethanol were added, followed by a short centrifugation step (3–4 s at 8000 rpm). The gold particles were washed three times with ice cold 100 % ethanol and taken up in a total volume of 40 μl. The particles were stored on ice until transformation.
Nuclear transformation was carried out according to Jakobiak et al., 2004 . One aliquot of gold particles were used to transform V. carteri spheroids from one Fernbach flask. For this, the gold particle mixture was spread over the center of 6 macrocarriers (fixed in their metal carriers). The macrocarriers were warmed on a heating block at 37 °C to evaporate residual ethanol. One macrocarrier, stopping screen and rupture disk (900 psi) were placed into the apparatus. The V. carteri spheroids were harvested using a sieve and spread in its center. The sieve was placed directly below the macrocarriers. Transformation was carried out under vacuum using the biolistic PDS 1000/He particle gun (BioRad Laboratories, Hercules, USA). For one transformation, the V. carteri spheroids were six times spread on the sieve, bombarded with gold particles and submerged in V. carteri medium during change of the carriers and disks.
Following bombardment, the spheroids were split into ten petri dishes containing 30 ml medium. Two days after transformation, 30 μg/ml paromomycin (selection marker) was added to each plate. After selection of paromomycin positive clones, the concentration was decreased to 10 μg/ml.
Cloning and sequencing of RNA transcripts
The RNA Seq libraries were generated from 4 μg total RNA per sample. The TruSeq Sample Preparation Kit (Illumina) was used according to the manufacturer’s instructions. The only derivation of the protocol was the substitution of the Superscript II reverse transcriptase with the Superscript III reverse transcriptase (both Life Technologies). Accordingly, the cDNA synthesis temperature was raised from 42 °C to 50 °C. Libraries of two biological replicates were generated on different days and sequenced on a HiSeq 2000 at the facilities of Illumina in a 100 bp paired end run. Additionally, the same libraries were sequenced in single runs (100 bp) with GATC (Konstanz, Germany). For the generation of strand-specific libraries, samples of all cells in all life stages were pooled. Total RNA was enriched for mRNA using the polyA Purist Kit (Life Technologies Cat No AM1919). Library was made with SOLiD total RNA-seq kit (Life Technologies Cat No 4445374) in accordance with manufacturer’s protocol and sequence on SOLiD 3 platform.
Northern blotting was performed as described before . In short, 5–10 μg of total RNA were run on a 12 % urea gel (UreaGel System, National diagnostics). For determining the approximate size of the small RNAs, ribooligonucleotides with a length of 19, 21 and 24 nucleotides (nt) were labeled with 32P prior to loading. The gel was run for 1 h at 250–350 V and semi-dry blotted onto an Amersham Hybond-N membrane (GE Healthcare) at 20 V for 30 min. Cross-linking with EDC-solution was performed at 50 °C for 1 h. The membrane was subsequently rinsed in water, dried and incubated with hybridization solution at 50 °C. After pre-hybridization, a radiolabeled probe antisense to the target small RNA was added and incubated over night at 50 °C. For labeling, 20 pmol of the probe (DNA oligonucleotide) were incubated with 20 μCi of 32P in a T4 PNK reaction (Fermentas) and it was purified with a G-25 column (GE Healthcare). After the incubation, the membrane was washed twice with 5xSSC, 1 % SDS, once with 1xSSC, 1 % SDS and wrapped in saran. The detection of signals was performed by the exposure to a screen and scanning with the PMI (Biorad).
For the re-probing of a membrane, the membrane was incubated with hot water with 0.1 % SDS for at least 15 min on a tumbler. The membrane was exposed to a screen for at least overnight to control for residual signal.
For the assessment of band heights when the radioactive marker had faded, the distance between the blue dye band heights was measured (marked with pencil on the membrane before blotting) as well as the distances of the marker bands to the blue dye bands. These figures were used to estimate band heights in later blots.
Analysis of RNA-Seq data
All small RNA sequencing data were adapter- and quality score-trimmed (trailing/leading base Phred score > 20). Resulting reads were aligned to the V. carteri reference genome version 9.0  using tophat2/bowtie2 .
Paired-end total RNA sequencing data were also adapter- and quality score-trimmed (trailing/leading base Phred score > 30), and aligned to the same V. carteri reference genome version 9.0.
Paired-end and single-end reads from libraries V1-V12 were mapped each separately to the V. carteri genome assembly vc199 (Phytozome v.8 ) using TopHat software v.1.4.1  and separate de novo transcriptome assemblies were generated for each library using Cufflinks software V 2.0.1  with default parameters. Next, all assemblies were merged using cuffmerge program from the Cufflinks package. Finally, transcript orientation was corrected by mapping strand-specific SOLiD RNA-seq data on the transcripts using Bowtie software v.0.12.7  and assigning particular orientation if the number of reads mapping to one strand was at least two times greater than the number of reads mapping to the opposite strand. In cases where this was not possible, no strand correction was performed.
Identification of transcripts with a putative role in RNAi
HHblits , which is part of the HHsuite, was used to search for fragments of RNAi processing proteins in the transcriptome of V. carteri. We used the current transcript assembly v.2 of the JGI (Joint Genome Institute), which is freely available at the Phytozome 10 database . In addition, the transcriptome data generated by us was used for this analysis (see paragraph above). HHblits requires for each protein to be searched for a hidden-Markov-model (HMM) to be generated by the user. These HMMs constitute a custom database, which was compiled according to the protocol detailed in chapter 3.4 of the HHSuite User Guide (downloaded from https://toolkit.tuebingen.mpg.de). To initiate the compilation of the respective HHMs, the following proteins from Arabidopsis thaliana were used as a seed: DCL4 (UniProtKB ID P84634), DCL1 (Q9SP32), HEN1 (Q9C5Q8) and RdRP6 (Q9SG02). For the search of additional Argonaute genes, the modified V. carteri AGO3 was used. For each sequence, a multiple sequence alignment was created by using HHblits applied to the database uniprot20_2013_03 from the EBI (http://www.uniprot.org/). As required, secondary structure was predicted by means of psipred_3.5  and added to the HHblits alignments. The compilation of the database was finalized according to the above-mentioned protocol. Since the open reading frames of annotated V. carteri proteins were not always supporting full-length proteins with start and stop codons, different protein sequences were created. DNA was translated in the six putative reading frames to protein sequences by means of methods from the Biophython package (https://github.com/biopython/biopython.github.io/). HHblits hits with an E-value ≤ 10E−5 were considered important and further processed. Next, the genomic locus of each transcript was extracted and the list of transcripts was sorted accordingly. Transcripts were grouped according to coverage of the query protein, e.g. for Argonaute, two transcripts in close proximity with one encoding the N-terminal and the other encoding the C-terminal part were considered to be an important hit. To further validate the candidate genes, the protein search algorithm of Pfam version 27.0  and Panther HMM Sequence Scoring  were employed to identify single important domains in the respective transcripts.
The miRNA identification was performed using the novel miRNA identification tool miRA . miRA does not assume sequence conservation, and was developed to characterize the miRNA landscape in organisms exhibiting heterogeneous miRNA precursor populations. Key parameters were chosen based on an analysis of miRBase-annotated miRNAs in C. reinhardtii. Details involving the identification method can be found in Evers et al. .
Annotation of other small RNAs
Reads overlapping annotated exons of mRNA transcripts were assigned to the mRNA fraction. Gene annotations were based on the V. carteri reference genome version 9.0 . Since there are no databases listing V. carteri rRNA and tRNA genes in full, reads were mapped against the database entries for C. reinhardtii tRNAs (PlantRNA database,  and rRNAs from A. thaliana (SILVA database,  and the order Volvocales (exported from GenBank, NCBI). Repeats were assigned using Repbase Update 19.02 , phased RNAs were predicted using the ta-si prediction tool from the UEA small RNA Workbench  which is based on the algorithm by Chen et al. .
miRNA target prediction
Potential miRNA targets in V. carteri were identified by requiring the following set of matching rules between the miRNA seed region and complementary mRNA binding site (adapted from ): (a) the binding site of the miRNA should have no more than four mismatches, (b) there should not be a mismatch at positions 10 an 11 of the miRNA, because complementarity here is required for cleavage, (c) there should not be adjacent mismatches at positions 2–12, (d) no more than two adjacent mismatches for positions > 12 are allowed and (e) there should be no bulge in the miRNA.
Mapping of small RNAs to repetitive elements
To investigate transcription of small RNAs from repetitive elements, the following strategy was employed: Small RNA reads were mapped to a list of RepBase-derived V. carteri repeat elements using tophat2/bowtie2. Overall the AgoIP sequencing library showed the strongest repeat element-associated expression, with Kangaroo, Gypsy3–5, and Jordan being amongst the most highly expressed repeat elements in the sample.
Generation of logos
All sequence logos were based on the adapter- and quality score-trimmed reads, and were generated using the R package motifStack (Ou and Zhu, R package version 1.12.0).
Generation of multiple sequence alignment
All sequences of miRNAs of V. carteri and C. reinhardtii (miRBase) were subjected to a multiple sequence alignment using ClustalW . The resulting matrix of alignment quality scores was plotted as a heat map of the pairwise score as percentage identity. The same analysis was performed with miRNAs from the liverwort Pellia endiviifolia .
Strains and culture conditions of V. carteri and C. reinhardtii
The female Volvox carteri f. nagariensis wild-type strain HK10 was originally obtained from R.C. Starr (Culture Collection of Algae, University of Texas, Austin, Texas, USA). The strain Vol6♀ was obtained from A. Hallmann (University of Bielefeld, Germany). Synchronous cultures were grown in Volvox medium at 28 °C under an 8 h dark/16 h light (10 000 lux) cycle . The sex-inducing pheromone was used as described by Haas and Sumper .
The cell-wall deficient strain CW15 mt- was obtained from Jörg Nickelsen (University of Munich) and cultivated on agar plates from TAP medium at room temperature on a shelf exposed to natural sunlight. TAP medium and its ingredients were made as described at chlamy.org, originally described in Gorman et al. . Liquid cultures were grown in TAP medium in Erlenmeyer flasks at room temperature and exposed to natural sunlight.
Immunoprecipitation and protein blots
Approximately 1 ml (myc-VcAGO3 transformed) or 400 μl (myc-CrGFP transformed) of V. carteri spheroids were lysed in 5 ml or 2 ml of lysis buffer (150 mM KCl, 25 mM Tris/HCl pH7.4, 0.5 % NP-40, 2 mM EDTA, 1 mM NaF), respectively, and sonicated twice for 30s (cycle 5, 10 % intensity, MS72 tip, Bandelin Sonoplus). After centrifugation at 17000 g, 4 °C and 30 min, the supernatant was flash frozen in liquid nitrogen. After thawing on ice the next day, the lysates were incubated with anti-c-myc-beads (Sigma) and incubated for 2.5 h at 4 °C on a rotating wheel. Beads were washed three times with wash buffer (300 mM KCl, 50 mM Tris/HCl pH7.4, 1 mM MgCl2, 0.1 % NP-40) and once with PBS.
For protein blots, 10 μl of the respective lysate as input and 20 % of the immunoprecipitation (IP) of the myc-VcAGO3 transformation and all of the myc-CrGFP transformation was used. For the control of immunoprecipitation of the cleavage assays, 10 % of each reaction was used.
Standard protein blots were performed using a 10 % separating gel. Semi-dry blotting using Towbin buffer was performed with 1.5 mA/cm2 for 2 h on Hybond ECL membrane (GE Healthcare). After blocking for 30 min with 5 % milk powder in TBS-T (TBS with 0.2 % Tween20), the membrane was incubated with the primary antibody (anti-myc, Sigma, 1:1500; anti-Flag 1:1000) over night at 4 °C. After washing with TBS-T, the membrane was incubated with the secondary antibody (anti-rabbit IRDye 800CW, Licor, 1:10000) for 1 h at room temperature. After washing, the membrane was scanned on a Licor Odyssey reader.
For the extraction of RNA from somatic cells or reproductive cells, 3 ml peqGold Trifast (Peqlab) or Trizol (Life technologies) was added per 100 μl cell suspension. Cells were lysed at room temperature for 10 min and RNA preparation was then carried out according to the manufacturer’s instructions. Precipitation of the RNA was performed over night at −20 °C in the presence of 20 μg glycogen (RNA grade, Life technologies) per ml of Trifast/Trizol. The RNA was collected by centrifugation, washed once with cold 80 % ethanol and solved in pure water.
RNA from immunoprecipitation samples was prepared as described .
Before assessing the cleavage activity, AGO proteins and the negative control CrGFP were precipitated via their respective tags. For VcAGO3 and CrGFP, the IP was carried out with 0.5 ml pellets as described under section “Immunoprecipitation and protein blots”. For lysing Arabidopsis tissue, 250 mg of tissue powder were incubated with 1 ml of ice cold extraction buffer (50 mM Tris HCl pH 7.5, 150 mM NaCl, 10 % Glycerol, 5 mM MgCl2, 0.1 % NP-40, 5 mM DTT), mixed well and incubated on a rotating wheel at 4 °C for 1 h. After clearing the cell debris by centrifugation (17,000 g, 4 °C, 30 min), 1 ml of lysate was added to 30 μl of packed Flag-beads (anti-Flag M2, Sigma) and incubated for 2 h at 4 °C on a rotating wheel. After three washes with wash buffer (50 mM Tris HCl pH 7.5, 500 mM NaCl, 10 % Glycerol, 5 mM MgCl2, 0.1 % NP-40, 4 mM DTT), PBS was used to split the immunoprecipitated AtAGO1 into fresh tubes. For checking the success of immunoprecipitation, 10 % of each IP (Volvox and Arabidopsis) were used for Western blotting.
The preparation of 32P-cap-labeled target RNA was performed as described before . For VcAGO3 cleavage assays, targets were generated carrying a perfectly complementary site to the small RNAs tasiRNA#1, MIR357b and sRNA scaff 12, while target RNAs for AtAGO1 cleavage assays carried sites for MIR159a and MIR165. The in vitro cleavage reaction was carried out with 50 % (v/v) immunoprecipitated protein (beads) in 66.7 mM KCl, 6.7 mM MgCl2, 8.3 mM DTT, 1.7 mM ATP, 0.3 mM GTP and 3.2 U RiboLock RNase inhibitor (Thermo Scientific). The addition of target RNA (1–2 Bq/cm2) initiated the reaction which was carried out for 1.5 h at 25 °C. The RNA was subsequently extracted using Proteinase K digestion and phenol-chloroform extraction as described before .
To visualize the cleavage products, samples were run on an 8 % urea polyacrylamide sequencing gel (National diagnostics), dried on Whatman paper and exposed to a screen.
Cloning and sequencing of small RNAs
Small RNAs from total RNA samples were converted into sequencing libraries as described before . The libraries were sequenced by Fasteris SA (Geneva, Switzerland) on an Illumina HiSeq2000 in a 50 bp single-end run. The RNA that was extracted from a VcAGO3 immunoprecipitation was cloned as described before  and sequenced on a MiSeq (Illumina) in a 66 bp single-end run.
β-elimination treatment of small RNAs
Total RNA from vegetative somatic cells and vegetative gonidia was mixed with 20 pmol of a random oligo RNA (5′-UUAGUGAGAGUCCAAUUAAUU-3′, Biomers). Beta-elimination was performed as described previously .
13.5 μl of total RNA (10–20 μg, vegetative somatic cells or vegetative gonidia) were mixed with 4.5 μl of 5x borate buffer (148 mM borax, 148 mM boric acid, pH 8.6) and 2.5 μl of freshly dissolved 200 mM NaIO4. After incubation for 10 min at room temperature, 2 μl of glycerol were added to quench unreacted NaIO4. Samples were incubated for another 10 min at room temperature and then dried by vacuum centrifugation for 1 h at room temperature.
Samples were dissolved in 50 μl 1x borax buffer (30 mM borax, 30 mM boric acid, 50 mM NaOH, pH 9.5) and incubated at 45 °C for 90 min. 20 μg glycogen was added to each sample and the RNA was precipitated with 2.5 volumes of ethanol at −20 °C over night. RNA was collected by centrifugation at 17000 g, 4 °C, 30 min. Pellets were directly dissolved in RNA loading dye.
- A. thaliana :
- C. reinhardtii :
GFP codon-optimized for C. reinhardtii
Trans-acting short interfering RNA
- V. carteri :
Volvox carteri ARGONAUTE 3
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We thank Gerhard Lehmann for help with data analysis, Corinna Friederich and Sigrun Ammon for excellent technical help, Dr. T. Demircan for help in preparing strand-specific RNAseq library, Rüdiger Schmitt for discussion and Jörg Nickelsen for providing the C. reinhardtii strain cw15 mt-. The Arabidopsis tissue powder was a kind gift from the Brodersen lab.
Our research is supported by grants from the Deutsche Forschungsgemeinschaft (SFB960 and FOR2127 to G.M.; SFB1101 to D.W.), the European Research Council (ERC grant 242792 ‘sRNAs’, ITN RNATrain), the Bavarian Genome Research Network (BayGene), the Bavarian Systems-Biology Network (BioSysNet) and the Max-Planck-Society (D.W.).
Availability of data and materials
Small RNA sequencing data is available at GEO under accessions GSE58703, GSE58704 and GSE59469. RNA Seq data is available at the SRA under BioProject PRJNA266874.
AD and GM conceived the study and designed experiments. AD, KU, NE and SW performed experiments. ME, SRH, RM and EB performed computational analyses. AD, DW, SW and GM analyzed and discussed data. AD, ME, JCE, DW, SW and GM wrote the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
Read count and mapping efficiencies of small RNA and RNA-Seq libraries used in this study. Most libraries could be mapped with more than 80 % of all reads. Rep = replicate. (PDF 165 kb)
Gene structure and putative domain structure of proteins involved in small RNA biogenesis. (A) Description of a second full length Argonaute protein in V. carteri. Potential transcripts at this locus are shown in green, the annotation by the Joint Genome Institute (JGI) as well as our own transcriptome assembly (“Cufflinks assembly”) were analyzed. The transcripts marked by a blue rectangle are overlapping and could manually be assembled to yield the transcript shown in black and marked with a red rectangle. The putative domain structure of the protein is depicted below. (B) The domain structure of a Dicer-like protein was assembled using several transcripts marked here in red rectangles. (C) Transcript and domain structure of a putative RNA-dependent RNA Polymerase (RdRP). (D) Transcript and domain structure of a putative HEN-1 homolog. (PDF 58 kb)
Full list of identified miRNA sequences in this study. The table shows the location and sequence of each miRNA within the genome, also the position of the precursor (pre) is given. Since sometimes multiple miRNAs originated from one precursor, an assignment was made in order to be able to correctly pair and differentiate each mature and star sequence. (PDF 433 kb)
Categorization of small RNA reads. (A) All six total small RNA libraries (vegetative somatic cells, induced somatic cells, female somatic cells, vegetative gonidia, induced gonidia and female egg cells were mapped according to Fig. 1E. In short, reads overlapping annotated exons of mRNA transcripts were assigned to the mRNA fraction. For the annotation of tRNAs and rRNAs, reads were mapped against the database entries for C. reinhardtii tRNAs and rRNAs from A. thaliana and the order Volvocales. Repeats were assigned using Repbase Update 19.02 , phased RNAs were predicted using the ta-si prediction tool from the UEA small RNA Workbench  which is based on the algorithm by Chen et al. . MiRNAs were predicted using the tool miRA . RE, repetitive elements. (B) Mapped reads from the libraries in (A) are shown according to Fig. 1F, i.e. categories are depicted without the putative intergenic reads. RE, repetitive elements. (PDF 42 kb)
MiRNA target sites on Jordan. Two exemplary target sites are shown, where a miRNA that originates from a Jordan element targets also a Jordan element. The miRNA is highlighted in grey and shown in 3′ to 5′ sequence. Part of the respective target sequence is shown below, lines indicate pairing of bases. The numbers on the right give the genome coordinates of the respective Jordan element. (PDF 36 kb)
Small RNA coverage of repetitive elements. All seven small RNA libraries (VcAGO3 IP (“AgoIP”), vegetative somatic cells (“asx_som”), induced somatic cells (“ind_som”), female somatic cells (“fem_som”), vegetative gonidia (“asx_gon”), induced gonidia (“ind_gon”) and female egg cells (“fem_egg”) were mapped to the consensus sequence of all repetitive elements deposited in Repbase19.02 (see also main Figs. 3 and 4). Each page shows the coverage of one repetitive element. Each graph depicts the read abundance, i.e. coverage by nucleotide (y-axis) over the length of the repetitive element (x-axis). Coverage on the plus strand is depicted in red, coverage on the minus strand in blue. Ctot = total count. (PDF 2101 kb)
Normalized expression of repetitive elements that are targeted by miRNAs. The diagrams show the normalized expression of the elements measured by RNA-Seq in the six conditions: Vegetative somatic cells (“som,veg”), induced somatic cells (“som,ind”), female somatic cells (“som,fem”), vegetative gonidia (“rep,veg”), induced gonidia (“rep,ind”) and female egg cells (“rep,fem”). (PDF 46 kb)
Multiple sequence alignment of V. carteri miRNAs with (A) C. reinhardtii miRNAs or (B) miRNAs of the liverwort Pellia endiviifolia. The alignment was constructed using ClustalW . The color code indicates the measure of similarity between the sequences. Red indicates no or low similarity, a light yellow or white shows a high similarity. The plot on the right depicts the frequencies of similarity over the whole data set. No sequence with conservation/high similarity could be found. (PDF 1889 kb)