- Research article
- Open Access
A gene-rich, transcriptionally active environment and the pre-deposition of repressive marks are predictive of susceptibility to KRAB/KAP1-mediated silencing
- Sylvain Meylan†1, 2,
- Anna C Groner†1, 2,
- Giovanna Ambrosini1, 3,
- Nirav Malani4,
- Simon Quenneville1, 2,
- Nadine Zangger1, 2,
- Adamandia Kapopoulou1, 2,
- Annamaria Kauzlaric1, 2,
- Jacques Rougemont1,
- Angela Ciuffi5,
- Frederic D Bushman4,
- Philipp Bucher1, 3 and
- Didier Trono1, 2Email author
© Meylan et al; licensee BioMed Central Ltd. 2011
- Received: 12 April 2011
- Accepted: 26 July 2011
- Published: 26 July 2011
KRAB-ZFPs (Krüppel-associated box domain-zinc finger proteins) are vertebrate-restricted transcriptional repressors encoded in the hundreds by the mouse and human genomes. They act via an essential cofactor, KAP1, which recruits effectors responsible for the formation of facultative heterochromatin. We have recently shown that KRAB/KAP1 can mediate long-range transcriptional repression through heterochromatin spreading, but also demonstrated that this process is at times countered by endogenous influences.
To investigate this issue further we used an ectopic KRAB-based repressor. This system allowed us to tether KRAB/KAP1 to hundreds of euchromatic sites within genes, and to record its impact on gene expression. We then correlated this KRAB/KAP1-mediated transcriptional effect to pre-existing genomic and chromatin structures to identify specific characteristics making a gene susceptible to repression.
We found that genes that were susceptible to KRAB/KAP1-mediated silencing carried higher levels of repressive histone marks both at the promoter and over the transcribed region than genes that were insensitive. In parallel, we found a high enrichment in euchromatic marks within both the close and more distant environment of these genes.
Together, these data indicate that high levels of gene activity in the genomic environment and the pre-deposition of repressive histone marks within a gene increase its susceptibility to KRAB/KAP1-mediated repression.
- KRAB-zinc finger proteins
- transcriptional repression
- histone modifications
Gene expression is modulated through the alteration of chromatin states by epigenetic regulators. Krüppel-associated box zinc finger proteins (KRAB-ZFPs), which together constitute the single largest group of transcriptional repressors encoded by the human genome, partake in this process [1–3]. The KRAB-ZFP family is evolutionary recent and has expanded and diverged through multiple rounds of gene and segment duplications, to give rise to more than three hundred and fifty annotated members in humans [4–7]. Despite their abundance, KRAB-ZFPs and their transcriptional targets remain largely uncharacterized except for a few [8–10]. KRAB-ZFPs carry a C-terminal array of two to forty C2H2 zinc finger motifs, each potentially capable of recognizing a triplet of nucleotides in a sequence-specific manner , while their N-terminal KRAB domain recruits the KAP1 (KRAB associated protein 1) corepressor [11–14]. KAP1 (also named TIF1β, KRIP-1 or TRIM28) binds KRAB and homotrimerizes through its N-terminal RBCC (Ring finger/B box/Coiled-Coil) domain, while its C-terminus acts as a scaffold for various heterochromatin-inducing factors, such as heterochromatin protein 1 (HP1), the histone methyltransferase ESET (also known as SetDB1), the nucleosome-remodeling and histone deacetylation (NuRD) complex, the nuclear receptor corepressor complex 1 (N-CoR1) and, at least during early embryonic development, de novo DNA methyltransferases [15–22]. This results in local loss of histone acetylation, enrichment in histone 3 lysine 9 trimethylation (H3K9me3) and increased chromatin compaction [23, 24].
Using chromatin immunoprecipitation (ChIP) and a tiling array, KAP1 has been documented to bind more than 7000 sites in a human testicular embryonal carcinoma cell line . A more recent publication additionally revealed that KAP1 chromatin targeting falls into different categories, only a subset of which is dependent on its RBCC domain and consequently on its association with KRAB-ZFPs . KAP1 is dynamically associated with both heterochromatin and euchromatin. It is thought to organize constitutive heterochromatin and to stimulate its propagation, as evidenced by its co-localization with HP1 in pericentromeric heterochromatin domains [16, 27]. Using a combination of gene trapping and a drug-controllable KRAB-containing repressor, we recently demonstrated that KRAB/KAP1 can induce long-range repression through HP1-dependent heterochromatin spreading . However, while some promoters located tens of kilobases (kb) from KAP1 docking sites were silenced by this mechanism, others were resistant. Here, we investigated the basis for this differential behavior by comparing the genomic context and the pre-existing levels of specific chromatin marks at repressed and non-repressed genes. This analysis revealed that genes most susceptible to KRAB/KAP1-induced silencing were in genomic regions of high gene activity. More specifically, repression was most efficient at sites with increased levels of pre-existing repressive histone marks at promoters and gene bodies, embedded within gene-rich regions with high levels of transcription.
Characterization of thousands of KRAB/KAP1-targeted gene traps
Since we were interested in elucidating differences between KRAB/KAP1 repressible and non-repressible promoters and genes, we reasoned that "all or none" phenotypes would facilitate subsequent analyses. Therefore, we selected cells in which trapped promoters were highly active at baseline, and either strongly repressed ("repressed clones" containing a "repressing integrant") or almost completely resistant to this process ("non-repressed clones" containing a "non-repressing integrant") when the trans-repressor was allowed to bind its target (Figure 1B). More specifically, we isolated trapped integrants from a population of cells by puromycin selection in the presence of Dox, which impairs tTRKRAB binding and silencing. Then trapped integrants were subjected to subsequent rounds of cell sorting to isolate cells harboring gene traps with repressible promoters and reporter genes. These rounds first included the isolation of GFP negative cells when tTRKRAB was allowed to bind (Dox-), followed by the sorting out of GFP positive cells when its recruitment was inhibited (Dox+) (Figure 1B). Isolation of non-repressible genes was achieved by a similar approach. However, trapped cell populations were cultured in the presence of tTRKRAB binding (Dox-) and GFP positive cells, which did not silence reporter expression, were directly isolated after TrapSil vector infections (Figure 1B).
After the isolation of cell populations with differential silencing phenotypes, we mapped proviral integration sites, in order to identify the trapped genes. For this, we combined linker-mediated PCR (LM-PCR) of proviral-genomic junctions with massive parallel DNA pyrosequencing [31, 33, 34]. The amplified sites were mapped to the human genome with the FetchGWI software , and the UCSC known gene annotation was used to subsequently identify the trapped promoters (Figure 1C). We previously described that about 1 in 15 promoters trapped by MLV-TrapSil vectors were non-repressed by tTRKRAB, compared with approximately 1 in 5 for those captured by LV-based vectors . Therefore, we isolated over 7000 integration sites, with an intentional bias for non-repressed clones to obtain integrant numbers comparable to their repressible counterparts. 69% of the promoter-trapping LV integrants mapped within annotated genes, whereas only 54% of their MLV counterparts did (Figure 1C, Additional File 1). This observation is in agreement with previous data indicating that parental MLV as well as MLV-based gene traps integrate in promoter proximal regions, which are less well annotated than gene bodies, which in turn are the preferential integration sites of LV and LV-based traps [36, 37]. Consistently, we mapped 6135 LV-TrapSil integrants to the genome, 4219 of which were located within genes. In contrast, we only found 787 intragenic MLV-TrapSil integrants.
Prior to further analysis, we validated our experimental approach by deriving clones from each population. All of the 32 clones analyzed exhibited the expected silencing profile in flow cytometry measurements. Moreover, the clones comprised 10 non-repressed (LI I-X) and 8 repressed (LR I-VIII) LV-TrapSil clones, in addition to 8 non-repressed (MI I-VIII) and 6 repressed (MR I-VI) MLV-TrapSil clones, (Additional File 2). We also used ChIP analysis to verify that non-repressed genes properly recruited KAP1 and downstream effectors to their tTRKRAB docking site, in a doxycycline-dependent manner (Additional File 3). After this validation, we continued with the characterization of the genomic context of our KRAB/KAP1 repressible or non-repressible genes to find patterns correlating with silencing efficiency.
Genomic environment of repressing and non-repressing gene trap integrants
We characterized the genomic environment of the integrants segregated according to their phenotype by using ROC (Receiver Operator Characteristic) curve analysis . This type of analysis was previously used to identify the genomic features enriched around retroviral integration sites. This study confirmed that both MLV and LV preferentially integrate within transcriptionally active regions, and that this effect is augmented when integrants enabling reporter expression are selected . In addition, this analysis also revealed that the effects of different genomic features on integration can change depending on the size of genomic segments in question . Therefore, we included genomic intervals ranging from 0.1 kb to 10 Mb in our analyses.
We then compared the ROC values, which are proportional to the levels of genomic features at these sites, between TrapSil groups harboring differential susceptibilities to KRAB/KAP1-silencing. We did this by making relative comparisons between a chosen reference and other gene groups. The reference gene groups are indicated by the symbol "---" within the whole results section. Using this approach we compared the levels of specific genomic features between the respective REP gene group and their corresponding NREP counterpart. When statistical differences were assessed, we found that LV-TrapSil repressing integrants were located within gene-denser genomic regions than non-repressible integrants (Figure 2). Furthermore, the environment of repressing LV integrants was enriched in CpG and DNase I sites, as well as in highly expressed genes (based on publicly available microarray data), compared to that of non-repressing LV integrants. While all the described parameters were statistically significantly different between repressible and non-repressible LV traps, comparisons of their MLV-TrapSil counterparts did not reach significant differences, although it showed similar trends (Figure 2). Therefore, a positive correlation between gene activity in the environment of the targeted transcriptional unit and efficient KRAB/KAP1-mediated silencing is established. The lack of significance between the MLV repressible and non-repressible TrapSil groups could be due to smaller integrant numbers or could reflect the presence of other uncharacterized features affecting KRAB/KAP1 recruitment, including the on average closer proximity of MLV integrants to promoters.
Genomic features of matched repressed and non-repressed transcriptional units
The genomic context of the three gene groups was reminiscent of observations made in the integrant-centered analysis (Figure 2), with genes from group 1 being in gene-richer and transcriptionally more active environments, and surrounded by a higher density of DNase I hypersensitivity sites (Figure 3B). These associations, however, did not reach statistical significance. Importantly, no difference in distance between repressor binding site and the trapped promoter was apparent when comparing the three groups, eliminating concerns about this potential bias for subsequent analyses of these genes (Figure 3B). When we examined the expression levels of the different gene groups, we found that genes supporting long-range repression (group 1) were on average more highly expressed than genes that did not enable KRAB/KAP1-mediated repression (group 3) (Figure 3C). Therefore, KRAB/KAP1-mediated silencing seems to be more effective in regions of high gene activity. To further consolidate this result, we assessed the levels of different chromatin features, correlating with transcriptional activation or repression in our different gene groups.
Chromatin features of matched repressed and non-repressed transcriptional units
We first assessed the levels of putative barrier elements such as CTCF, H3.3/H2Az or chromatin modifiers in the different groups [39–41]. This was achieved by utilizing published datasets, which were used to calculate the relative abundance of these features by ROC curve analysis and by comparing these values between the groups. There was no differential association with either one of the three gene groups for the intervals tested (Additional File 5).
Previous analyses on the mechanisms of KRAB/KAP1-mediated gene regulation have mostly examined the impact of this system on the expression of transfected promoter-reporter units. Here, we investigated KRAB/KAP1-induced changes within the context of endogenous genes. Using a combination of promoter trapping and drug-controllable KRAB/KAP1 recruitment, we previously observed that this complex, when docked to the bodies of transcriptionally active genes, could induce silencing over distances of several tens of kilobases . However, we had also noted that repression was more efficient if the distance between the effector and the promoter was less than 20 kb. Furthermore, a significant fraction of trapped promoters/KRAB docking loci escaped these rules, suggesting other counteracting influences. The present large-scale comparison of the genomic features of KRAB/KAP1-responsive and KRAB/KAP1-resistant transcriptional units identified by our gene trap system reveals a positive correlation between efficient KRAB/KAP1-mediated repression of trapped promoters and i) a gene-richer and transcriptionally more active genomic context, ii) a more euchromatic environment, and iii) the pre-existence of some repressive marks at and around the promoter.
Comparing KRAB/KAP1-repressed and non-repressed genes gave no indication for a role of putative obstacles to the spread of heterochromatin, such as CTCF binding, accumulation of H3.3/H2Az or recruitment of HATs (reviewed in [42, 43]). This is consistent with the observation that CTCF recruitment to the HS4 region of the chicken β-globin locus can be prevented without abrogating the barrier function of this DNA sequence . However, it is at odds with a recent study presenting CTCF as a marker of transition between euchromatic and heterochromatic regions . A model reconciling these findings would be that CTCF acts as an H3K27me3 heterochromatin-specific barrier yet has no effect on H3K9me3-based heterochromatin propagation. However, it should be emphasized that our analysis was limited to the transcribed region of genes owing to our gene trap-based approach, precluding overly general conclusions on the possible role of barrier elements.
Although both repressed and non-repressed genes were situated within euchromatic regions, as expected from the promoter-trapping approach used for their selection, we observed significant differences in both their local and broader chromatin environments. Repressed genes were in regions containing generally higher levels of major euchromatin-associated marks and higher levels of transcription compared with non-repressed genes. Therefore, there is a positive correlation between efficient KRAB/KAP1-silencing and high gene activity. This is suggestive of a model whereby genes situated in more heterochromatic environments can only be highly expressed if endowed with an intrinsic ability to resist repressive influences, while genes located in more euchromatic environments do not need such protective mechanisms . Consistently, in our analysis KRAB/KAP1-resistant units were on average closer to telomeres than their KRAB/KAP1-susceptible counterparts, although this difference did not reach statistical significance (data not shown).
Genes repressed by the TrapSil system also carried higher levels of the repressive marks H4K20me3 and H9K9me3 at baseline at and around their promoters, compared with their repression-resistant counterparts. Noteworthy, these contrasting chromatin configurations were not only observed when comparing a selected set of multiply hit repressed and non-repressed genes (Figure 5), but were also present in the complete pools of repressing and non-repressing integrants (Additional File 6). Interestingly, a recent analysis of the chromatin structure of zinc finger genes found that high levels of both H3K36me3 and H3K9me3 co-localized at the 3' exons of these genes . Since KRAB-ZFP genes, which belong to this gene family, are endogenous targets of KRAB/KAP1-repression [25, 47], we performed the same analysis in our HeLa cell system and reproduced the same result (Additional Files 7 and 8). Therefore, the high levels of both H3K9me3 and H3K36me3 at KRAB-ZFP gene bodies may be necessary for efficient KRAB/KAP1-induced heterochromatin spreading. The finding that the repressive H3K9me3 and the activating H3K36me3 marks are not co-regulated further supports this hypothesis , since high levels of H3K36me3, which positively correlate with active transcription, may independently enhance the spread of H3K9me3 at KRAB-ZFP genes. This model is reminiscent of results obtained from the TrapSil analysis, where high levels of both active and repressive histone marks can be seen in genes that accommodate KRAB/KAP1-mediated heterochromatin spreading and silencing.
A difference between genes targeted by our TrapSil system and endogenous KRAB-ZFP genes lays in the finding that the latter do not seem susceptible to KRAB/KAP1-mediated long-range repression [28, 46, 47]. This may be due to the use of our ectopic repressor system. Alternatively, certain endogenous promoters may be resistant to KRAB/KAP1-induced heterochromatin spreading. A possible factor in this process is the H3K9me1/2 demethylase PHF8 . Active H3K9 demethylation may prevent the heterochromatization of KRAB-ZFP promoters and subsequent transcriptional silencing. This idea is consistent with recent PHF8 genome-wide binding data that showed it locating to the promoter regions of zinc finger-encoding genes .
Other mechanisms potentially involved in conferring resistance to KRAB/KAP1-mediated silencing are suggested by the analysis of genes that were hotspots of proviral TrapSil targeting and carried both repressible and non-repressible integrants (Additional File 9). In this subgroup, the repressible integrants generally clustered closer to the promoter than their non-repressible counterparts, consistent with the overall observation that silencing is most efficient when KRAB/KAP1 is recruited in the proximity of the affected promoter. In some cases, however, the distributions of repressible and non-repressible integrants overlapped within the same gene. This could reflect the differential susceptibilities of the two alleles of a gene to KRAB/KAP1-mediated repression, somewhat reminiscent of what is observed with imprinting, a process that involves a KRAB-ZFP [9, 50]. Additionally, cells within a population may be heterogeneous for the chromatin status of specific loci, which in turn might impact on the consequences of KRAB/KAP1 recruitment. Such a phenomenon would be comparable to variegation, where particular genes are differentially expressed amongst cells of an otherwise apparently homogeneous population .
In summary, the present work indicates that the impact of KRAB-mediated docking of KAP1 on the expression of targeted genes is more variable than previously suspected. It further reveals reciprocal influences between the functional outcome of KRAB/KAP1 recruitment to DNA and the chromatin features of the involved loci. More broadly, the approach described in the present study, which combined an analysis of the functional consequences of exogenously introduced cis-acting KRAB/KAP1-recruiting sequences with an examination of the transcriptional activity, genomic context and chromatin features of targeted loci, could be fruitfully applied to the study other epigenetic regulators.
pLV-tTR-KRAB-Red was previously described . pLtTR-KRAB-NG95 was cloned through ligation of a BamHI/XhoI digested MLV-based pNG95  with a compatible tTR-KRAB amplicon with BamHI/XhoI sites added by PCR (primer sequences see Additional File 10). To construct LV- and MLV-based TrapSil vectors, published gene trap vectors  were modified by PCR-based mutagenesis (Stratagene mutagenesis kit). A BlpI restriction site was introduced into the MLV U3 region of 3'LTRs (MLV: BlpI Primers MLV Trap F/R - Additional File 10), whereas a SpeI site was introduced in the LV U3 region of 3'LTR (Primers HIV Trap F/R - Additional File 10), these new sites were then used to insert 7 repeats of TetO. LV- and MLV-based particles were produced and titered as described elsewhere http://tcf.epfl.ch/page-6764-en.html. The WPRE of LV-TRAPSIL, the GAG remnant of MLV-TRAPSIL, and the Albumin gene served for proviral and cellular genome quantification by Taqman.
Cell culture and Fluorescence activated cell sorting (FACS)
HeLa cells were grown under standard conditions. Doxycycline (Sigma-Aldrich) was used at a concentration of 1 μg/mL. Clonal tTRKRAB-expressing HeLa cell lines dsRK4 (pLV-tTR-KRAB-Red, LV-backbone) and KiN1.25 (pLTetR-KRAB-NG95, MLV-backbone) were derived after infection with pLV-tTR-KRAB-Red or pTetR-KRAB-NG95, respectively. The LV based HeLa dsRK4 clone contains approx. 15 vector copies as titrated by Taqman and was used for MLV-TRAPSIL assays while the MLV-based KiN1.25 clone contains 10 vector copies and was used for all LV-TRAPSIL assays. In view of this mapping strategy, 2 × 108 dsRK4 or KiN1.25 HeLa cells were infected with 1.6 × 106 MLV-TrapSil or LV-TrapSil infectious particles, respectively, with a multiplicity of infection of 0.04. Cells were sorted based for GFP expression by using the Beckton Dickinson FACSVantage SE turbo Sorter with Diva Option. Flow Cytometry analyses were performed on BD FACScan flow cytometer.
Quantitative PCR (qPCR)
qPCR reactions were carried out with a standard PCR program in ABI PRISM 7900 HT in duplicates or triplicate using either SYBR green detection 1× Power Sybr or 1× Taqman Universal Mix, No AmpErase (Applied Biosystems). Primers were used at a final concentration of 100 nM. When SYBR analysis was performed, cycling reactions were followed by a dissociation curve analysis to validate specificity of amplified products. The increase in fluorescence was analyzed with the SDS software, version 2.2.2 (Applied Biosystems). For all amplification plots the baseline data were set with the automatic cycle threshold function. Primer sequences for all qPCR reactions are listed in Additional File 10.
Linker-mediated PCR (LM-PCR), 454 pyrosequencing and data processing
LM-PCR was used to map integration sites following a previously described protocol [31, 33, 34]. Briefly, 10 μg of genomic DNA (DNeasy, Qiagen) was digested with MseI. Fragments were ligated to a linker and were digested with DpnI and SacI (LV-TrapSil) or SpeI (MLV-TrapSil) to avoid contaminations with bacterial plasmids and to avoid cloning of internal vector fragments. Nested PCR then served to amplify TrapSil vector-gDNA junctions (Takara Advantage 2 kit). Amplicons ranging between 100 and 400 bp were purified, quantified and sent for pyrosequencing at GATC biotech (Konstanz, Germany). Raw sequences were downloaded from the GATC biotech website and converted to FASTA files. Sequences having exact pyrosequencing reaction primers (F: primer A; R: primer B, Additional File 10) were selected and others discarded. Selected sequences were then categorized according to barcode for TrapSil vector type and integrant type (barcodes: LI: TGAC/AGTC; LR: CTGA; MI: TCGA/AGCT; MR: GTAC). After classification, all primer sequences and viral vector overhangs were trimmed yielding only genomic DNA sequence. The 20 bases adjacent to primer B before trimming were used as tags for mapping the inserts to the human genome assembly hg18. The mapping was done using FetchGWI tolerating at most 2 mismatches .
Integration site mapping in genes: Integrant orientation was annotated as determined during sequence processing. UCSC known gene  were downloaded from UCSC tables with transcript start (Tsx), transcript end (Tsend) and gene orientation. Only integrants mapping with correct orientation within a gene were mapped relative to it. In a second step, a non-redundant gene list was generated (from the original UCSC Gene list) using an aggressive clustering strategy, which groups all transcripts that directly or indirectly (through other transcripts) overlap on the same strand of the same chromosome. In the non-redundant gene list we recorded the 5'most Tsx position and the 3'most Tsend position for each cluster. For analysis of the integrant distance to gene promoters, we considered only integrants falling within the transcribed region of the same gene. Files containing integration sites (sequence-mapping from Insipid, LV_LUI (LV irrepressible), LV_LUR (LV repressible), MLV_MUI (MLV irrepressible), MLV_MUR (MLV_repressible)) and gene groups (20 KB promoter classes with at least 3 integration sites: group 1: "long-range repressible), group 2: "short-range repressible", group 3: "long-range irrepressible") can be found under http://ccg.vital-it.ch/KAP1/.
Receiver Operator Characteristic (ROC) curve analysis
Data analysis was based on a "nested case control" strategy using a collection of TSS characterized by a given behavior with respect to repression along with control sites sampled from the genome to make inferences about the probability of a TSS to display a given response to repression based on genomic/epigenetic features characterizing its environment. More detailed description of statistical basis for this analysis can be found in . Data were analyzed using the R language and environment for statistical computing/graphics version 2.3.0 and several contributed packages. Empirical ROC curve areas were calculated for datasets that used random genomic controls, in which case each TSS of a cluster was compared only with its matched controls to determine the proportions of controls whose values equaled or exceeded that of TSS . Annotations of genomic features were obtained as described previously ; the chromatin features analyzed came from ChIP-seq data generated in this and other studies [39–41].
Chromatin immunoprecipitation (ChIP) and ChIP followed by sequencing (ChIP-Seq)
ChIP reactions were performed according to published protocols with minor modifications (http://www.millipore.com/userguides/tech1/mcproto407 and http://cshprotocols.cshlp.org/cgi/content/full/2009/6/pdb.prot5237), using antibodies listed in Additional File 11, either native or pre-bound to beads. For Histone modifications, 2 × 107 HeLa cells were trypsinized and resuspended in MNase buffer. 1 U MNase (Roche) was added for 10 min and adding EDTA to a final of 10 mM arrested the nuclease reaction. Chromatin was sonicated with a Branson digital sonicator (model 250) on ice three times for 20 s and then dialyzed against RIPA with AEBSF protease inhibitor 0.2 mM for 1 h. The chromatin was pelleted after dialysis; glycerol was added to the supernatant to a final 5% concentration and the chromatin was stored at -80°C. 500 ul was incubated with AB-specific pre-coated beads over night (IP). Complexes were washed, eluted, purified, precipitated and resuspended in 50 ul H2O. For KAP1 ChIPs, approximately 2 × 107 cells were cross-linked with 1% formaldehyde for 8 min at RT, quenched by adding glycine and rinsed with PBS, before shearing by sonication with a Branson digital sonicator (model 250) on ice four times for 20 s at 30% intensity. 100 μl of sonicated chromatin was directly de-crosslinked and used as the total input (TI) reference in qPCR analysis at a dilution of 1:100. 100 μl of sonicated chromatin was used for each ChIP reaction and was diluted in 900 μl dilution buffer and precleared with 80 μl salmon-sperm DNA protein A agarose beads (Upstate). Chromatin-antibody complexes were captured washed and eluted with 100 mM NaHCO3, 1% SDS. Cross-links between DNA and proteins were reversed by addition of NaCl and incubation at 65°C. DNA was precipitated after incubation with RNase A (Sigma) and Proteinase K (Roche) and resuspended in 50 μl H2O and subjected to qPCR analysis. qPCR is described above and primers are listed in Additional File 10. Negative control reactions without antibody were run for each sample and in all cases gave negligible results. To validate the relative enrichment of proteins or specific histone modifications at a given sequence a ratio between the relative quantities of IP and TI was established.
The sequencing libraries from all ChIP products were prepared using the ChIP-seq Sample Preparation Kit (Illumina; San Diego, California, USA; Cat. No. IP-102-1001) according to the protocol supplied with the reagents and using 10 ng of ChIP sample quantified using the Qubit fluorometer (Invitrogen; Carlsbad, California, USA). One lane of each library was sequenced on the Illumina Genome Analyzer IIx using the Single-Read Cluster Generation Kit v2 (Cat. No. FC-103-2001) and 36 Cycle Sequencing Kits v3 and v4 (Cat. Nos. FC-104-3002 and FC-104-4002). Data were processed using the Illumina Pipeline Software v1.5.1. Illumina GAII data were mapped to the genome with Bowtie http://bowtie-bio.sourceforge.net/index.shtml. All output files were converted to processable file formats (SGA) for subsequent bioinformatics analysis described below. Enrichment of genomic and chromatin features was assessed with the ChIPcor web-based tool http://ccg.vital-it.ch/chipseq/chip_cor.html. All files were converted into SGA format, settings included: sort input: on, strand option: oriented for references files (gene clusters TSS and poly-A sites) and any for all target features, range was set at -40 kb to +40 kb, window size was at 500/50 (for graphic or statistical analysis) and cut-off value was 1. Raw and processed sga files can also be found under http://ccg.vital-it.ch/KAP1/ and were used as follows: "SGA files for Zhao-produced CTCF and H3K27me3 genome wide" include HeLa-CTCF.sga.gz, HeLa-H3K27me3.sga.gz data; "H3.3-H2A.Z double ChIP (Zhao et al)" contain GSM335958.sga.gz files; "SGA files from genome-wide mapping of HATs and HDACs in human CD4+ T cells" : contain CD4-Tip60.sga.gz; "SGA files for histone modification profiles (ChIP-Seq data)": contains H3K27ac: H3K27acpf.sga, H2BK5me1: H2BK5me1pf.sga, H3K4me1: H3K4me1pf.sga, H3K4me3: H3K4me3p.sga, H3K36me3: H3K36me3b.sga, H4K20me1: H4K20me1b.sga, H3K9me2: H3K9me2p.sga, H3K9me3: H3K9me3pf.sga, H4K20me3: H4K20me3b.sga.
HU133a arrays for HeLa cells were downloaded from GEO/NCBI [56, 57] and data were extracted and normalized using RNA Robust Multichip Average (Quantile normalization) . Specific gene expression scores were extracted and normalized average values for 4 different arrays were calculated. Statistical comparisons were done with the non-parametric Wilcoxon test.
We thank Joanna Roberts and Miguel Garcia for the FACS analyses, and Keith Harshman and colleagues for help with the DNA sequencing. This work was supported by grants from the Swiss National Science Foundation, the Strauss Foundation, the European Union (PERSIST program) and the Infectigen Association to DT, by an MD-PhD scholarship from the Swiss National Science Foundation to SM, and by NIH grants AI52845 and AI082020, by the University of Pennsylvania Center for AIDS Research and by the Penn Genome Frontiers Institute through a grant from the Pennsylvania Department of Health to F.D.B.
- Urrutia R: KRAB-containing zinc-finger repressor proteins. Genome Biol. 2003, 4 (10): 231-10.1186/gb-2003-4-10-231.View ArticlePubMedPubMed CentralGoogle Scholar
- Ding G, Lorenz P, Kreutzer M, Li Y, Thiesen HJ: SysZNF: the C2H2 zinc finger gene database. Nucleic Acids Res. 2009, D267-273. 37 DatabaseGoogle Scholar
- Emerson RO, Thomas JH: Adaptive evolution in zinc finger transcription factors. PLoS Genet. 2009, 5 (1): e1000325-10.1371/journal.pgen.1000325.View ArticlePubMedPubMed CentralGoogle Scholar
- Huntley S, Baggott DM, Hamilton AT, Tran-Gyamfi M, Yang S, Kim J, Gordon L, Branscomb E, Stubbs L: A comprehensive catalog of human KRAB-associated zinc finger genes: insights into the evolutionary history of a large family of transcriptional repressors. Genome Res. 2006, 16 (5): 669-677. 10.1101/gr.4842106.View ArticlePubMedPubMed CentralGoogle Scholar
- Tadepally HD, Burger G, Aubry M: Evolution of C2H2-zinc finger genes and subfamilies in mammals: species-specific duplication and loss of clusters, genes and effector domains. BMC Evol Biol. 2008, 8: 176-10.1186/1471-2148-8-176.View ArticlePubMedPubMed CentralGoogle Scholar
- Vaquerizas JM, Kummerfeld SK, Teichmann SA, Luscombe NM: A census of human transcription factors: function, expression and evolution. Nat Rev Genet. 2009, 10 (4): 252-263. 10.1038/nrg2538.View ArticlePubMedGoogle Scholar
- Lorenz P, Dietmann S, Wilhelm T, Koczan D, Autran S, Gad S, Wen G, Ding G, Li Y, Rousseau-Merck MF, Thiesen HJ: The ancient mammalian KRAB zinc finger gene cluster on human chromosome 8q24.3 illustrates principles of C2H2 zinc finger evolution associated with unique expression profiles in human tissues. BMC Genomics. 2010, 11: 206-10.1186/1471-2164-11-206.View ArticlePubMedPubMed CentralGoogle Scholar
- Zheng L, Pan H, Li S, Flesken-Nikitin A, Chen PL, Boyer TG, Lee WH: Sequence-specific transcriptional corepressor function for BRCA1 through a novel zinc finger protein, ZBRK1. Mol Cell. 2000, 6 (4): 757-768. 10.1016/S1097-2765(00)00075-7.View ArticlePubMedGoogle Scholar
- Li X, Ito M, Zhou F, Youngson N, Zuo X, Leder P, Ferguson-Smith AC: A maternal-zygotic effect gene, Zfp57, maintains both maternal and paternal imprints. Dev Cell. 2008, 15 (4): 547-557. 10.1016/j.devcel.2008.08.014.View ArticlePubMedPubMed CentralGoogle Scholar
- Tian C, Xing G, Xie P, Lu K, Nie J, Wang J, Li L, Gao M, Zhang L, He F: KRAB-type zinc-finger protein Apak specifically regulates p53-dependent apoptosis. Nat Cell Biol. 2009, 11 (5): 580-591. 10.1038/ncb1864.View ArticlePubMedGoogle Scholar
- Margolin JF, Friedman JR, Meyer WK, Vissing H, Thiesen HJ, Rauscher FJ: Kruppel-associated boxes are potent transcriptional repression domains. Proc Natl Acad Sci USA. 1994, 91 (10): 4509-4513. 10.1073/pnas.91.10.4509.View ArticlePubMedPubMed CentralGoogle Scholar
- Friedman JR, Fredericks WJ, Jensen DE, Speicher DW, Huang XP, Neilson EG, Rauscher FJ: KAP-1, a novel corepressor for the highly conserved KRAB repression domain. Genes Dev. 1996, 10 (16): 2067-2078. 10.1101/gad.10.16.2067.View ArticlePubMedGoogle Scholar
- Kim SS, Chen YM, O'Leary E, Witzgall R, Vidal M, Bonventre JV: A novel member of the RING finger family, KRIP-1, associates with the KRAB-A transcriptional repressor domain of zinc finger proteins. Proc Natl Acad Sci USA. 1996, 93 (26): 15299-15304. 10.1073/pnas.93.26.15299.View ArticlePubMedPubMed CentralGoogle Scholar
- Moosmann P, Georgiev O, Le Douarin B, Bourquin JP, Schaffner W: Transcriptional repression by RING finger protein TIF1 beta that interacts with the KRAB repressor domain of KOX1. Nucleic Acids Res. 1996, 24 (24): 4859-4867. 10.1093/nar/24.24.4859.View ArticlePubMedPubMed CentralGoogle Scholar
- Le Douarin B, Nielsen AL, Garnier JM, Ichinose H, Jeanmougin F, Losson R, Chambon P: A possible involvement of TIF1 alpha and TIF1 beta in the epigenetic control of transcription by nuclear receptors. Embo J. 1996, 15 (23): 6701-6715.PubMedPubMed CentralGoogle Scholar
- Ryan RF, Schultz DC, Ayyanathan K, Singh PB, Friedman JR, Fredericks WJ, Rauscher FJ: KAP-1 corepressor protein interacts and colocalizes with heterochromatic and euchromatic HP1 proteins: a potential role for Kruppel-associated box-zinc finger proteins in heterochromatin-mediated gene silencing. Mol Cell Biol. 1999, 19 (6): 4366-4378.View ArticlePubMedPubMed CentralGoogle Scholar
- Underhill C, Qutob MS, Yee SP, Torchia J: A novel nuclear receptor corepressor complex, N-CoR, contains components of the mammalian SWI/SNF complex and the corepressor KAP-1. J Biol Chem. 2000, 275 (51): 40463-40470. 10.1074/jbc.M007864200.View ArticlePubMedGoogle Scholar
- Peng H, Begg GE, Schultz DC, Friedman JR, Jensen DE, Speicher DW, Rauscher FJ: Reconstitution of the KRAB-KAP-1 repressor complex: a model system for defining the molecular anatomy of RING-B box-coiled-coil domain-mediated protein-protein interactions. J Mol Biol. 2000, 295 (5): 1139-1162. 10.1006/jmbi.1999.3402.View ArticlePubMedGoogle Scholar
- Schultz DC, Friedman JR, Rauscher FJ: Targeting histone deacetylase complexes via KRAB-zinc finger proteins: the PHD and bromodomains of KAP-1 form a cooperative unit that recruits a novel isoform of the Mi-2alpha subunit of NuRD. Genes Dev. 2001, 15 (4): 428-443. 10.1101/gad.869501.View ArticlePubMedPubMed CentralGoogle Scholar
- Schultz DC, Ayyanathan K, Negorev D, Maul GG, Rauscher FJ: SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins. Genes Dev. 2002, 16 (8): 919-932. 10.1101/gad.973302.View ArticlePubMedPubMed CentralGoogle Scholar
- Cammas F, Herzog M, Lerouge T, Chambon P, Losson R: Association of the transcriptional corepressor TIF1beta with heterochromatin protein 1 (HP1): an essential role for progression through differentiation. Genes Dev. 2004, 18 (17): 2147-2160. 10.1101/gad.302904.View ArticlePubMedPubMed CentralGoogle Scholar
- Wiznerowicz M, Jakobsson J, Szulc J, Liao S, Quazzola A, Beermann F, Aebischer P, Trono D: The Kruppel-associated box repressor domain can trigger de novo promoter methylation during mouse early embryogenesis. J Biol Chem. 2007, 282 (47): 34535-34541. 10.1074/jbc.M705898200.View ArticlePubMedGoogle Scholar
- Ayyanathan K, Lechner MS, Bell P, Maul GG, Schultz DC, Yamada Y, Tanaka K, Torigoe K, Rauscher FJ: Regulated recruitment of HP1 to a euchromatic gene induces mitotically heritable, epigenetic gene silencing: a mammalian cell culture model of gene variegation. Genes Dev. 2003, 17 (15): 1855-1869. 10.1101/gad.1102803.View ArticlePubMedPubMed CentralGoogle Scholar
- Sripathy SP, Stevens J, Schultz DC: The KAP1 corepressor functions to coordinate the assembly of de novo HP1-demarcated microenvironments of heterochromatin required for KRAB zinc finger protein-mediated transcriptional repression. Mol Cell Biol. 2006, 26 (22): 8623-8638. 10.1128/MCB.00487-06.View ArticlePubMedPubMed CentralGoogle Scholar
- O'Geen H, Squazzo SL, Iyengar S, Blahnik K, Rinn JL, Chang HY, Green R, Farnham PJ: Genome-wide analysis of KAP1 binding suggests autoregulation of KRAB-ZNFs. PLoS Genet. 2007, 3 (6): e89-10.1371/journal.pgen.0030089.View ArticlePubMedPubMed CentralGoogle Scholar
- Iyengar S, Ivanov AV, Jin VX, Rauscher FJ, Farnham PJ: Functional analysis of KAP1 genomic recruitment. Mol Cell Biol. 2011Google Scholar
- Loyola A, Tagami H, Bonaldi T, Roche D, Quivy JP, Imhof A, Nakatani Y, Dent SY, Almouzni G: The HP1alpha-CAF1-SetDB1-containing complex provides H3K9me1 for Suv39-mediated K9me3 in pericentric heterochromatin. EMBO Rep. 2009, 10 (7): 769-775. 10.1038/embor.2009.90.View ArticlePubMedPubMed CentralGoogle Scholar
- Groner AC, Meylan S, Ciuffi A, Zangger N, Ambrosini G, Denervaud N, Bucher P, Trono D: KRAB-zinc finger proteins and KAP1 can mediate long-range transcriptional repression through heterochromatin spreading. PLoS Genet. 2010, 6 (3): e1000869-10.1371/journal.pgen.1000869.View ArticlePubMedPubMed CentralGoogle Scholar
- Deuschle U, Meyer WK, Thiesen HJ: Tetracycline-reversible silencing of eukaryotic promoters. Mol Cell Biol. 1995, 15 (4): 1907-1914.View ArticlePubMedPubMed CentralGoogle Scholar
- Moosmann P, Georgiev O, Thiesen HJ, Hagmann M, Schaffner W: Silencing of RNA polymerases II and III-dependent transcription by the KRAB protein domain of KOX1, a Kruppel-type zinc finger factor. Biol Chem. 1997, 378 (7): 669-677. 10.1515/bchm.1997.378.7.669.View ArticlePubMedGoogle Scholar
- Schroder AR, Shinn P, Chen H, Berry C, Ecker JR, Bushman F: HIV-1 integration in the human genome favors active genes and local hotspots. Cell. 2002, 110 (4): 521-529. 10.1016/S0092-8674(02)00864-4.View ArticlePubMedGoogle Scholar
- Mitchell RS, Beitzel BF, Schroder AR, Shinn P, Chen H, Berry CC, Ecker JR, Bushman FD: Retroviral DNA integration: ASLV, HIV, and MLV show distinct target site preferences. PLoS Biol. 2004, 2 (8): E234-10.1371/journal.pbio.0020234.View ArticlePubMedPubMed CentralGoogle Scholar
- Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA, Berka J, Braverman MS, Chen YJ, Chen Z, Dewell SB, Du L, Fierro JM, Gomes XV, Godwin BC, He W, Helgesen S, Ho CH, Irzyk GP, Jando SC, Alenquer ML, Jarvie TP, Jirage KB, Kim JB, Knight JR, Lanza JR, Leamon JH, Lefkowitz SM, Lei M, Li J, Lohman KL, Lu H, Makhijani VB, McDade KE, McKenna MP, Myers EW, Nickerson E, Nobile JR, Plant R, Puc BP, Ronan MT, Roth GT, Sarkis GJ, Simons JF, Simpson JW, Srinivasan M, Tartaro KR, Tomasz A, Vogt KA, Volkmer GA, Wang SH, Wang Y, Weiner MP, Yu P, Begley RF, Rothberg JM: Genome sequencing in microfabricated high-density picolitre reactors. Nature. 2005, 437 (7057): 376-380.PubMedPubMed CentralGoogle Scholar
- Wang GP, Ciuffi A, Leipzig J, Berry CC, Bushman FD: HIV integration site selection: analysis by massively parallel pyrosequencing reveals association with epigenetic modifications. Genome Res. 2007, 17 (8): 1186-1194. 10.1101/gr.6286907.View ArticlePubMedPubMed CentralGoogle Scholar
- Iseli C, Ambrosini G, Bucher P, Jongeneel CV: Indexing strategies for rapid searches of short words in genome sequences. PLoS One. 2007, 2 (6): e579-10.1371/journal.pone.0000579.View ArticlePubMedPubMed CentralGoogle Scholar
- Lewinski MK, Yamashita M, Emerman M, Ciuffi A, Marshall H, Crawford G, Collins F, Shinn P, Leipzig J, Hannenhalli S, Berry CC, Ecker JR, Bushman FD: Retroviral DNA integration: viral and cellular determinants of target-site selection. PLoS Pathog. 2006, 2 (6): e60-10.1371/journal.ppat.0020060.View ArticlePubMedPubMed CentralGoogle Scholar
- De Palma M, Montini E, Santoni de Sio FR, Benedicenti F, Gentile A, Medico E, Naldini L: Promoter trapping reveals significant differences in integration site selection between MLV and HIV vectors in primary hematopoietic cells. Blood. 2005, 105 (6): 2307-2315. 10.1182/blood-2004-03-0798.View ArticlePubMedGoogle Scholar
- Berry C, Hannenhalli S, Leipzig J, Bushman FD: Selection of target sites for mobile DNA integration in the human genome. PLoS Comput Biol. 2006, 2 (11): e157-10.1371/journal.pcbi.0020157.View ArticlePubMedPubMed CentralGoogle Scholar
- Cuddapah S, Jothi R, Schones DE, Roh TY, Cui K, Zhao K: Global analysis of the insulator binding protein CTCF in chromatin barrier regions reveals demarcation of active and repressive domains. Genome Res. 2009, 19 (1): 24-32.View ArticlePubMedPubMed CentralGoogle Scholar
- Jin C, Zang C, Wei G, Cui K, Peng W, Zhao K, Felsenfeld G: H3.3/H2A.Z double variant-containing nucleosomes mark 'nucleosome-free regions' of active promoters and other regulatory regions. Nat Genet. 2009, 41 (8): 941-945. 10.1038/ng.409.View ArticlePubMedPubMed CentralGoogle Scholar
- Wang Z, Zang C, Cui K, Schones DE, Barski A, Peng W, Zhao K: Genome-wide mapping of HATs and HDACs reveals distinct functions in active and inactive genes. Cell. 2009, 138 (5): 1019-1031. 10.1016/j.cell.2009.06.049.View ArticlePubMedPubMed CentralGoogle Scholar
- West AG, Gaszner M, Felsenfeld G: Insulators: many functions, many mechanisms. Genes Dev. 2002, 16 (3): 271-288. 10.1101/gad.954702.View ArticlePubMedGoogle Scholar
- Gaszner M, Felsenfeld G: Insulators: exploiting transcriptional and epigenetic mechanisms. Nat Rev Genet. 2006, 7 (9): 703-713. 10.1038/nrg1925.View ArticlePubMedGoogle Scholar
- Recillas-Targa F, Pikaart MJ, Burgess-Beusse B, Bell AC, Litt MD, West AG, Gaszner M, Felsenfeld G: Position-effect protection and enhancer blocking by the chicken beta-globin insulator are separable activities. Proc Natl Acad Sci USA. 2002, 99 (10): 6883-6888. 10.1073/pnas.102179399.View ArticlePubMedPubMed CentralGoogle Scholar
- Hediger F, Gasser SM: Heterochromatin protein 1: don't judge the book by its cover!. Curr Opin Genet Dev. 2006, 16 (2): 143-150. 10.1016/j.gde.2006.02.013.View ArticlePubMedGoogle Scholar
- Blahnik KR, Dou L, Echipare L, Iyengar S, O'Geen H, Sanchez E, Zhao Y, Marra MA, Hirst M, Costello JF, Korf I, Farnham PJ: Characterization of the contradictory chromatin signatures at the 3' exons of zinc finger genes. PLoS One. 2011, 6 (2): e17121-10.1371/journal.pone.0017121.View ArticlePubMedPubMed CentralGoogle Scholar
- Frietze S, O'Geen H, Blahnik KR, Jin VX, Farnham PJ: ZNF274 recruits the histone methyltransferase SETDB1 to the 3' ends of ZNF genes. PLoS One. 2010, 5 (12): e15082-10.1371/journal.pone.0015082.View ArticlePubMedPubMed CentralGoogle Scholar
- Loenarz C, Ge W, Coleman ML, Rose NR, Cooper CD, Klose RJ, Ratcliffe PJ, Schofield CJ: PHF8, a gene associated with cleft lip/palate and mental retardation, encodes for an Nepsilon-dimethyl lysine demethylase. Hum Mol Genet. 2010, 19 (2): 217-222. 10.1093/hmg/ddp480.View ArticlePubMedGoogle Scholar
- Kleine-Kohlbrecher D, Christensen J, Vandamme J, Abarrategui I, Bak M, Tommerup N, Shi X, Gozani O, Rappsilber J, Salcini AE, Helin K: A functional link between the histone demethylase PHF8 and the transcription factor ZNF711 in X-linked mental retardation. Mol Cell. 2010, 38 (2): 165-178. 10.1016/j.molcel.2010.03.002.View ArticlePubMedPubMed CentralGoogle Scholar
- Mackay DJ, Callaway JL, Marks SM, White HE, Acerini CL, Boonen SE, Dayanikli P, Firth HV, Goodship JA, Haemers AP, Hahnemann JM, Kordonouri O, Masoud AF, Oestergaard E, Storr J, Ellard S, Hattersley AT, Robinson DO, Temple IK: Hypomethylation of multiple imprinted loci in individuals with transient neonatal diabetes is associated with mutations in ZFP57. Nat Genet. 2008, 40 (8): 949-951. 10.1038/ng.187.View ArticlePubMedGoogle Scholar
- Henikoff S: Position-effect variegation after 60 years. Trends Genet. 1990, 6 (12): 422-426.View ArticlePubMedGoogle Scholar
- Wiznerowicz M, Trono D: Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference. J Virol. 2003, 77 (16): 8957-8961. 10.1128/JVI.77.16.8957-8951.2003.View ArticlePubMedPubMed CentralGoogle Scholar
- Sheehy AM, Gaddis NC, Choi JD, Malim MH: Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein. Nature. 2002, 418 (6898): 646-650. 10.1038/nature00939.View ArticlePubMedGoogle Scholar
- Hsu JY, Sun ZW, Li X, Reuben M, Tatchell K, Bishop DK, Grushcow JM, Brame CJ, Caldwell JA, Hunt DF, Lin R, Smith MM, Allis CD: Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes. Cell. 2000, 102 (3): 279-291. 10.1016/S0092-8674(00)00034-9.View ArticlePubMedGoogle Scholar
- DeLong ER, DeLong DM, Clarke-Pearson DL: Comparing the areas under two or more correlated receiver operating characteristic curves: a nonparametric approach. Biometrics. 1988, 44 (3): 837-845. 10.2307/2531595.View ArticlePubMedGoogle Scholar
- Carson JP, Zhang N, Frampton GM, Gerry NP, Lenburg ME, Christman MF: Pharmacogenomic identification of targets for adjuvant therapy with the topoisomerase poison camptothecin. Cancer Res. 2004, 64 (6): 2096-2104. 10.1158/0008-5472.CAN-03-2029.View ArticlePubMedGoogle Scholar
- Cassani B, Montini E, Maruggi G, Ambrosi A, Mirolo M, Selleri S, Biral E, Frugnoli I, Hernandez-Trujillo V, Di Serio C, Roncarolo MG, Naldini L, Mavilio F, Aiuti A: Integration of retroviral vectors induces minor changes in the transcriptional activity of T cells from ADA-SCID patients treated with gene therapy. Blood. 2009, 114 (17): 3546-3556. 10.1182/blood-2009-02-202085.View ArticlePubMedGoogle Scholar
- Irizarry RA, Hobbs B, Collin F, Beazer-Barclay YD, Antonellis KJ, Scherf U, Speed TP: Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics. 2003, 4 (2): 249-264. 10.1093/biostatistics/4.2.249.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.