- Research article
- Open Access
Chromosome rearrangements, recombination suppression, and limited segregation distortion in hybrids between Yellowstone cutthroat trout (Oncorhynchus clarkii bouvieri) and rainbow trout (O. mykiss)
© Ostberg et al.; licensee BioMed Central Ltd. 2013
- Received: 22 February 2013
- Accepted: 16 August 2013
- Published: 22 August 2013
Introgressive hybridization is an important evolutionary process that can lead to the creation of novel genome structures and thus potentially new genetic variation for selection to act upon. On the other hand, hybridization with introduced species can threaten native species, such as cutthroat trout (Oncorhynchus clarkii) following the introduction of rainbow trout (O. mykiss). Neither the evolutionary consequences nor conservation implications of rainbow trout introgression in cutthroat trout is well understood. Therefore, we generated a genetic linkage map for rainbow-Yellowstone cutthroat trout (O. clarkii bouvieri) hybrids to evaluate genome processes that may help explain how introgression affects hybrid genome evolution.
The hybrid map closely aligned with the rainbow trout map (a cutthroat trout map does not exist), sharing all but one linkage group. This linkage group (RYHyb20) represented a fusion between an acrocentric (Omy28) and a metacentric chromosome (Omy20) in rainbow trout. Additional mapping in Yellowstone cutthroat trout indicated the two rainbow trout homologues were fused in the Yellowstone genome. Variation in the number of hybrid linkage groups (28 or 29) likely depended on a Robertsonian rearrangement polymorphism within the rainbow trout stock. Comparison between the female-merged F1 map and a female consensus rainbow trout map revealed that introgression suppressed recombination across large genomic regions in 5 hybrid linkage groups. Two of these linkage groups (RYHyb20 and RYHyb25_29) contained confirmed chromosome rearrangements between rainbow and Yellowstone cutthroat trout indicating that rearrangements may suppress recombination. The frequency of allelic and genotypic segregation distortion varied among parents and families, suggesting few incompatibilities exist between rainbow and Yellowstone cutthroat trout genomes.
Chromosome rearrangements suppressed recombination in the hybrids. This result supports several previous findings demonstrating that recombination suppression restricts gene flow between chromosomes that differ by arrangement. Conservation of synteny and map order between the hybrid and rainbow trout maps and minimal segregation distortion in the hybrids suggest rainbow and Yellowstone cutthroat trout genomes freely introgress across chromosomes with similar arrangement. Taken together, these results suggest that rearrangements impede introgression. Recombination suppression across rearrangements could enable large portions of non-recombined chromosomes to persist within admixed populations.
- Linkage Group
- Rainbow Trout
- Segregation Distortion
- Cutthroat Trout
- Metacentric Chromosome
The widespread occurrence of hybridization has been a catalyst for intensive study in evolutionary biology and has provided rich opportunities for investigating genome evolution, adaptation, speciation, reproductive isolation, and hybrid fitness[1–3]. Hybridization is a natural evolutionary process, contributing to the diversification of plants and animals[4, 5]. When populations hybridize and progeny are viable and fertile, genomes introgress and produce recombined chromosomes. Introgression breaks down linkage associations and generates novel gene combinations which may have fitness consequences. However, hybridization can also have significant conservation implications, leading to the formation of hybrid swarms and extinction[6, 7]. In these cases, introgression may interfere with epistatic interactions by disrupting local adaptations and breaking down co-adapted gene complexes. Regardless of the consequences, understanding the genome processes that affect introgression is fundamental to understanding hybrid genome evolution.
Genetic linkage maps provide a means for investigating genome evolution and function, and have contributed to an improved understanding of hybridization and introgression[8–10]. They have provided empirical evidence that chromosome rearrangements can act as barriers to gene flow by suppressing recombination between rearranged chromosomes[11–13]. They can be used to identify segregation distortion (loci that deviate from Mendelian inheritance patterns), which may indicate the presence of fitness-linked loci or genetic incompatibilities. When applied to introgressed populations, linkage maps have identified specific genomic regions that might be important for providing adaptive fitness advantages[9, 15]. Finally, they provide a framework for detecting quantitative trait loci (QTL), enabling identification of genomic regions associated with ecological, evolutionary, or physiological processes within hybrids and parental species[10, 16, 17].
Genome maps for salmonid fishes show signatures of two significant events: genome duplication and chromosome rearrangements[18–21]. The first significant event, genome duplication, is thought to have occurred through autopolyploidy approximately 25–100 million years ago, resulting in an ancestral tetraploid genome. Rediploidization of the genome is occurring but is not complete. This residual tetraploidy has two consequences exclusive to males. First, males form multivalents between homeologues during meiosis, suppressing crossing-over between homologues and reducing recombination rates compared to females. Second, residual tetraploidy results in pseudolinkage between homeologous chromosomes in males, producing statistical rather than physical linkage. The second significant event, chromosome rearrangements, has generated highly variable chromosome numbers among salmonid species. Although chromosome numbers differ among species, chromosome arm numbers have remained relatively constant because rearrangements have primarily been of the Robertsonian type between acrocentric and metacentric chromosomes. Robertsonian rearrangements are translocations that involve centric fusion or fission between chromosome arms, causing a change in chromosome number but not chromosome arm number.
Rainbow trout (Oncorhynchus mykiss) and cutthroat trout (O. clarkii) are two salmonid species that inhabit western North America. Rainbow and cutthroat trout are sister species and shared a common ancestor approximately 3 million years ago. Despite karyotypic differences between the species, Robertsonian rearrangements have maintained the same number (n = 104) of diploid chromosome arms[27, 28]. Similarity in chromosome arm number between the two species could be an important factor that enables the species to readily hybridize and produce viable and fertile progeny. In fact, where non-indigenous rainbow trout have been introduced into indigenous cutthroat trout habitats, introgressive hybridization has led to hybrid swarms and extinction of local cutthroat trout populations, and has thus become a major conservation concern. Although introgression between rainbow and cutthroat trout is well documented, it is unknown how introgression affects the genomic architecture of hybrids and thus their subsequent evolution. For example, karyotypic differences between rainbow and cutthroat trout could affect hybrid genome evolution by suppressing recombination, hindering gene flow, and generating linkage disequilibrium[10, 11, 13, 30]. As a result, the ability of invading alleles to become established within a host genome could be influenced by the presence of chromosome rearrangements. In addition, reduced recombination between rearranged chromosome segments could prevent disruption of co-adapted gene complexes[31, 32]; enabling these adaptations to persist within hybrid populations, which could ultimately affect hybrid fitness.
Here, we present the first hybrid genetic linkage map between two introgressing salmonid species, rainbow trout and Yellowstone cutthroat trout (O. c. bouvieri). Rainbow trout (RBT) have 58–64 diploid chromosomes depending on the chromosome race whereas Yellowstone cutthroat trout (YCT) have 64 diploid chromosomes. We generated F2 hybrids between RBT and YCT and developed a F1 hybrid linkage map to investigate the genomic consequences of introgression. The objectives for constructing the F1 hybrid linkage map were to 1) determine if linkage groups were conserved between the F1 map and existing RBT maps, 2) determine if introgression suppressed recombination, and 3) estimate the prevalence of segregation distortion in the F1 hybrid map. Our hybrid linkage map has application to conservation and management of indigenous cutthroat trout subspecies because it localizes species-specific markers to linkage groups and identifies genomic regions where recombination is suppressed, both of which may assist resource managers in determining accurate estimates of RBT admixture throughout the native cutthroat trout range. It will also have application for identifying QTL associated with species-specific traits. Finally, it can be used as a baseline for future comparative mapping studies in rainbow-cutthroat trout hybrids.
Hybrid linkage map
Hybrid linkage groups and alignment to rainbow trout chromosome (Omy) and linkage group (RT) arms ( p and q )
Hybrid linkage group
Inferred linkage group structure
Omy01 (p, q)
RT6 (p, q)
Omy02 (p, q)
RT27 (p, q)
Omy03 (p, q)
RT31 (p, q)
Omy04 (p, q)
RT24 (p, q)
Omy05 (p, q)
RT8 (p, q)
Omy06 (p, q)
RT10 (p, q)
Omy07 (p, q)
RT12 (p, q)
Omy08 (p, q)
RT23 (p, q)
Omy09 (p, q)
RT21 (p, q)
Omy10 (p, q)
RT20 (p, q)
Omy11 (p, q)
RT19 (p, q)
Omy12 (p, q)
RT9 (p, q)
Omy13 (p, q)
RT2 (p, q)
Omy14 (p, q)
RT3 (p, q)
Omy15 (p, q)
RT7 (p, q)
Omy16 (p, q)
RT22 (p, q)
Omy17 (p, q)
RT29 (p, q)
Omy18 (p, q)
RT16 (p, q)
Omy19 (p, q)
RT14 (p, q)
Omy20 (p, q)_Omy28 (q)
RT17 (p, q)_RT13 (q)
Omy21 (p, q)
RT15 (p, q)
Omy22 (p, q)
RT5 (p, q)
Omy25 (q)_Omy29 (q)
RT4 (q)_RT25 (q)
Marker order was largely conserved among parent-specific and between female- and male-merged maps (Additional file1, Worksheets 2 – 8; Additional files2 and3). However, we observed inconsistent marker order among parent-specific maps for RYHyb25 and RYHyb29 and, therefore, these two linkage groups could not be merged within sex. Further, marker order differed between sexes for distally mapping loci at RYHyb13p, RYHyb19, and RYHyb24. Loci with different order between sexes were removed prior to generating the sex-merged map, and removal did not appear to alter marker order within linkage groups. Marker order differences could be due to marker informativeness within families, insufficient chromosome coverage, or reduced recombination in males.
Assignment of species diagnostic markers to linkage groups
We assigned 114 diagnostic species markers (97 SNPs, 13 indels, and 4 restriction fragment length polymorphisms (RFLPs)) to specific linkage groups (Additional file1, Worksheets 9). Specific mapping locations within linkage groups could not be determined because the F1 parents were heterozygous at these loci. One indel, OCC-37, was polymorphic within YCT and mapped to a specific location within RYHyb20. All hybrid linkage groups contained at least one species-specific diagnostic marker. Linkage groups RYHyb21 and RYHyb11, both metacentric chromosomes, were each assigned a single diagnostic species marker indicating that only one of the two chromosome arms in both linkage groups had a species-specific marker assigned.
Using duplicated loci, we observed six homeologous hybrid linkage groups (RYHyb12q/RYHyb13q, RYHyb13p/RYHyb17p, RYHyb14p/RYHyb29, RYHyb15q/RYHyb21q, RYHyb10q/RYHyb19p, and RYHyb06p/RYHyb26) that have been previously identified within RBT. We also observed one homeologous pairing that has not been observed in rainbow trout (RYHyb03 centromere region/RYHyb22q). Additionally, for 10 other duplicated loci, only one homeologue could be scored confidently. Linkage groups RYHyb13q and RYHyb19p each contained two of these loci, and RYHyb02p, RYHyb02 centromere region, RYHyb10q, RYHyb10 centromere region, RYHyb18p, and RYHyb21q each contained one of these loci.
We found eight pseudolinkage groups exclusive to both male maps (RYHyb01/RYHyb23, RYHyb02/RYHyb03, RYHyb06/RYHyb26, RYHyb07/RYHyb18, RYHyb10/RYHyb19, RYHyb15/RYHyb21, RYHyb12/RYHyb13, and RYHyb13/RYHyb17). Pseudolinkage was represented in males by statistical linkage between markers that mapped to independent linkage groups in the two female maps. All cases of pseudolinkage were between chromosomes identified as homeologous within RBT.
Map distances, in centiMorgans (cM), in female and male merged hybrid maps, female to male recombination ratios (F:M), and significant allelic segregation distortion across 25 cM intervals for each linkage group (sliding window analysis)
Female map distance (cM)
Male map distance (cM)
Family 1 F:M ratio
Family 2 F:M ratio
Significant number of 25 cM intervals1
44.1 (10.8 + 33.3)2
84.5 (21.8 + 62.7)2
46.4 (46.4 + 0)2
76.1 (54.8 + 21.3)2
46.9 (8.6 + 38.3)2
We identified 13 linkage groups where recombination rates differed significantly between the female consensus F1 hybrid and RBT maps (Additional file4). We treated RYHyb20 as two separate linkage groups, noted as RYHyb20(Omy20) and RYHyb20(Omy28), because Omy20 and Omy28 are not fused in RBT. Further, RYHyb25 and RYHyb29 represent a fusion/fission polymorphism in the female maps, and so we treated these groups as independent in Female 1 and used the RBT linkage group in Guyomard et al. for comparison. We also treated RYHyb25_29 as a metacentric linkage group in Female 2 and used the map of Rexroad et al. for comparison.
We found 15 linkage groups where genotypic segregation distortion occurred at P < 0.05 (Additional file5). Approximately 6% of the total loci genotyped were distorted at this significance level. After applying B-Y FDR corrections for multiple tests, the observed genotypic frequencies differed significantly from expected frequencies within only four linkage groups (Additional file5), and represented approximately 1.6% of the total loci genotyped. Linkage groups RYHyb09 and RYHyb18 contained loci with an excess of YCT/YCT genotypes, and RYHyb06 and RYHyb07 contained loci with an excess of RBT/RBT genotypes. Interestingly, none of the 309 loci mapped in common between the two families showed significant genotypic distortion within both families.
The genetic linkage maps established for YCT-RBT hybrids provide novel insights into the genomic consequences of introgression between RBT and YCT. Hybrid and RBT linkage groups were syntenic and had similar marker order, suggesting that RBT and YCT share chromosome arms. In addition, hybrids and RBT shared linkage groups, with one exception where a hybrid linkage group involved a fusion between a bi-armed metacentric and a single-armed acrocentric RBT chromosome. This result was confirmed by additional mapping in YCT. Comparison between hybrid and RBT female maps indicated that introgression suppressed recombination across several large chromosome segments. Two hybrid linkage groups showing recombination suppression (RYHyb20 and RYHyb25_28) contained different chromosome arrangements between YCT and RBT. Segregation distortion was generally limited and distortion patterns varied among parents and families, suggesting that few incompatibilities exist between RBT and YCT genomes. Taken together, these results suggest that RBT and YCT genomes freely introgress, with the exception that differences in chromosome rearrangements between the species could impede introgression across large portions of specific linkage groups.
Chromosome rearrangements between RBT and YCT
The hybrid map is a product of differences in chromosome rearrangements between RBT and YCT, as well as mixed ancestry of the RBT source stock. Using data from karyotypes[27, 28] and the hybrid map, we suggest that RBT and YCT differ by at least five species-specific chromosome rearrangements. We first consider the fusion between Omy28 and Omy20q in YCT. The most parsimonious explanation for the fusion between the metacentric Omy20 and the acrocentric Omy28 involves two rearrangements. The first rearrangement would have occurred prior to the Omy20-Omy28 fusion as a pericentric inversion of the entire q-arm of Omy20. This inversion would have resulted in Omy20 becoming an acrocentric chromosome and a reduction in the expected 52 haploid chromosome arms[27, 28] to the observed 51 haploid arms in the hybrid map. The possibility that Omy20q could be inverted in YCT relative to RBT is supported in Chinook salmon (O. tshawytscha), as the Omy20 homologue in Chinook is an acrocentric chromosome and the segment homologous to Omy20q appears inverted compared to RBT (unpublished observations, K. Naish). The second rearrangement would have been a Robertsonian type involving centric fusion between the acrocentric Omy20 homologue and Omy28, conserving chromosome arm number. The three remaining differences may be explained by comparing acrocentric chromosome numbers between RBT (seven in the 60 chromosome race) and YCT (twelve). The seven RBT acrocentric chromosomes have been identified as such in the hybrid map, suggesting that they have an acrocentric YCT homologue. The remaining five YCT acrocentric chromosomes likely have homologs with RBT metacentric chromosomes. The third and fourth rearrangement differences probably represent centric fusion/fission events between two RBT metacentric and four of the five remaining YCT acrocentric chromosomes, which would not change the chromosome arm number. The fifth rearrangement difference may have involved a fission event within a RBT metacentric chromosome arm, generating the fifth acrocentric and a submetacentric chromosome in YCT. Fission within a RBT metacentric arm would gain one YCT chromosome arm and restore the 52 haploid chromosome arm number, countering the loss of a chromosome arm by the pericentric inversion.
The variable number of linkage groups identified in the hybrid maps (28 and 29) is likely due to mixed ancestry of the source RBT stock. Linkage analysis in YCT indicated that the Omy25 and Omy29 homologues each represent acrocentric linkage groups in YCT, but these chromosomes are known to be a Robertsonian polymorphism in RBT[28, 34, 35]. The Kamloops stock at Hayspur Hatchery appears to include ancestry from inland RBT (2 N = 58; Omy25 and Omy29 are fused as a metacentric chromosomes) as well as the common hatchery RBT derived from coastal California (2 N = 60; Omy25 and Omy29 are acrocentric chromosomes) (R. F. Leary, Montana Fish, Wildlife and Parks, personal communication). Admixture between the two RBT stocks would generate Robertsonian metacentric (2 N = 58) and acrocentric (2 N = 60) polymorphs, as well as Robertsonian heterozygotes (2 N = 59). Subsequent hybridization between the polymorphic Kamloops stock and YCT would produce F1 hybrids that were Robertsonian heterozygotes (for example, Female 2 and Male 1) comprised of 28 linkage groups, containing the RBT metacentric fusion Omy25_Omy29 and the YCT acrocentric homologues to Omy25 and Omy29, as well as Robertsonian acrocentric homozygotes (for example, Female 1 and Male 2) comprised of 29 linkage groups, containing RBT acrocentric chromosomes Omy25 and Omy28 and their YCT homologues (Figure 2).
Conclusions based on recombination differences between the hybrid and RBT maps have limitations, as rates can vary between related species, as well as among individuals within species[18, 19, 24]. Indeed, we found significant differences in pairwise recombination rates between the two RBT maps[21, 33] used to construct the female consensus map across Omy5, Omy8, Omy14, Omy19, Omy20, and Omy22. Furthermore, differences in marker density among hybrid and RBT maps could also account for recombination differences by affecting map distance estimates. However, our objective was to perform a comparative analysis to identify recombination suppression broadly across the genome. Because recombination frequency appears correlated with chromosome arm number and broad-scale recombination rates tend to be conserved between closely related species, we might expect similar recombination rates between RBT and YCT. Therefore, although absolute differences between map distances can be affected by number of markers and number of individuals mapped, the trend revealed by the comparative analyses yields interesting insight into recombination suppression in the hybrids, which could indicate the presence of chromosome rearrangements or genic incompatibilities.
Chromosome rearrangements can generate recombination suppression in heterokaryotypes (chromosomal hybrids) through unbalanced gametes which results in non-recombinants being the only viable gametes, or by restricting recombination between rearrangements. Although unbalanced gametes cannot be ruled out, we consider them an unlikely cause of suppression in the F1 hybrids. First, conservation of synteny and marker order between the hybrid and RBT maps[21, 33] suggests that YCT and RBT chromosomes are highly collinear, which would facilitate normal pairing between homologues and alternate disjunction in F1 hybrids. Second, because RBT and YCT contain the same number of chromosome arms, Robertsonian type rearrangements are considered to have played a significant role in generating the chromosome number differences between the species. Meiotic pairing between homologues that differ by a Robertsonian rearrangement (i.e., pairing between a metacentric chromosome and the two acrocentric homologues) would produce a trivalent in the F1 hybrids, which would not necessarily cause malsegregation and unbalanced gametes. For example, the rate of nondisjunction was not different in individuals that were heterozygous for Robertsonian rearrangements compared to homozygotes in pink salmon (O. gorbuscha), house mouse (Mus musculus domesticus), and Eurasian common shrew (Sorex araneus), suggesting this type of rearrangement produces balanced gametes.
Recombination was restricted in the F1 hybrids across several loci spanning chromosome fusion and fission differences between RBT and YCT; the Robertsonian fusion/fission within RYHyb25_29 and RYHyb20. This suggests that chromosome rearrangements did indeed suppress recombination. Rearrangements generate extensive linkage disequilibrium in heterokarotypic hybrids and suppress recombination across genomic regions that extend beyond rearrangements[11, 30, 43]. Therefore, broad-scale recombination suppression across other linkage groups in the F1 hybrids could indicate the presence of rearrangements. We suggest that three other metacentric hybrid linkage groups, RYHyb11, RYHyb14, and RYHyb15, contained chromosomes that differed by arrangement between RBT and YCT. These linkage groups might represent Robertsonian rearrangements because recombination was suppressed across putative centromeres which could indicate that F1 hybrids were heterozygous for centric fusions/fissions. However, we found marker order differences within the suppressed regions in each of these three linkage groups compared to RBT linkage maps[21, 33], which could possibly indicate inversions or translocations, or, alternatively, be due to reduced mapping power in regions with low recombination or the number of progeny used to construct the various maps.
Significant differences in recombination rates between several hybrid and RBT linkage groups could indicate the presence of inter-specific, genic incompatibilities. However, our broad-scale analysis hinders inference across smaller genomic scales where incompatibilities have been reported[44–46]. Nevertheless, we found numerous instances where two adjacent markers did not recombine in the female-merged hybrid map, but these same markers recombined in the consensus female RBT map. This might suggest that recombination between particular YCT and RBT genomic regions is maladaptive, or that the difference in adjacent recombination is an artifact of the number of offspring genotyped. Finer scale mapping across these regions that differ in recombination rate could be fruitful for identifying the presence of incompatibilities. Inter-specific incompatibilities may also be inferred from segregation distortion, as genotypes that occur less often than expected may be incompatible[3, 14]. In addition, we expected that inter-specific incompatibilities would show consistent distortion among maps. For example, consistent allelic distortion within both RYHyb01 female hybrid maps and both RYHyb09 male hybrid maps could indicate the presence of incompatibilities. Although segregation distortion was present in several other linkage groups, a lack of consistency across hybrid maps suggests these distorted loci do not reflect genic incompatibilities between RBT and YCT.
Recombination suppression within Robertsonian rearrangements
The recombination suppression pattern across the Robertsonian rearrangement RYHyb25_29 and the presumed Robertsonian rearrangement RYHyb15 differed from patterns reported between chromosome races in the house mouse[47, 48] and common shrew. Recombination in mice and shrews that were homozygous for Robertsonian fusions (i.e., homozygous for the metacentric polymorphism) appeared suppressed near centromeres and elevated toward telomeres in comparison to Robertsonian heterozygotes[39, 47]. However, we observed the opposite; recombination was suppressed in Robertsonian heterozygotes (i.e., hybrids) across the putative centromere and elevated toward telomeres in comparison to fusion homozygotes (i.e., the female RBT consensus map). The mechanisms causing the difference between our results and the house mouse and common shrew are unclear. Given the approximate 3 million year divergence time between RBT and YCT and the absence of historical secondary contact, genomic differences could have evolved near the centromeres of rearranged chromosomes that would restrict crossover events in Robertsonian heterozygotes, such as para- or pericentric inversions or genic incompatibilities. Alternatively, mechanistic processes governing meiotic crossover could differ among taxa. Determining how recombination suppression in the Omy25-Omy29 Robertsonian rearrangement differs between intra-specific (RBT chromosome races) and inter-specific hybrids could indicate the efficiency of this type of rearrangement as a barrier to gene flow in salmonids.
Rearrangements protect genomic regions from recombination
Our results suggest that chromosome rearrangement is the main genomic obstacle for gene exchange between RBT and YCT. Rearrangements have been observed to reduce gene flow between several species pairs, including Drosophila pseudoobscura and D. persimilis, Helianthus petiolaris and H. annuus, and S. araneus and S. antinorii[49, 50]. Rearrangements protect genomic regions from recombination, enabling genes within or closely linked to the rearranged genome to differentiate between heterokaryotypes while unrestricted gene flow occurs between regions with similar composition[45, 51]. As a result, fitness related genes could accumulate within regions that are protected from recombination and diverge in the face of hybridization[45, 51]. Indeed, genes involved with reproductive isolation have mapped to chromosome rearrangements[31, 32]. The effectiveness of rearrangements as recombination suppressors may be dependent on how chromosomes are reorganized; rearrangements that change gene order (e.g., inversions or translocations) may be more effective in protecting the genome from being disrupted than rearrangements that do not change gene order (e.g., fusions or fissions). However, several studies indicate that Robertsonian type rearrangements restrict gene flow[11, 49, 50, 52], but with extreme interbreeding this type of rearrangement may be an ineffective barrier.
Given the hybrid linkage map results, we predict that, within introgressed populations, inter-specific recombination will be restricted in particular genomic regions where chromosome arrangement differs between YCT and RBT. This prediction is supported by studies that have reported reduced gene flow across chromosome rearrangements within stable hybrid zones[10, 11, 50, 52]. Because the majority of hybrid linkage groups appeared to have similar marker order and arrangement with RBT, few chromosomes would be expected to contain regions with restricted recombination, although suppression could potentially extend broadly across these chromosomes[11, 30, 43]. Within a long standing hybrid zone between house mouse chromosome races, linkage disequilibrium persisted among loci mapping near Robertsonian rearrangements, indicating that these types of rearrangements can generate and maintain linkage disequilibrium. Given that RBT-YCT introgression is recent and rearrangements produce extensive linkage disequilibrium, large genomic regions that flank centric fusion/fission differences could be expected to remain intact within some YCT chromosomes. As a consequence, recombination suppression between heterokaryotypes could protect genomic regions from being disrupted and enable co-adapted gene complexes and/or local adaptations linked to the rearrangements to persist within YCT admixed with RBT.
Segregation distortion mechanisms
The low level of segregation distortion observed in the RBT-YCT hybrid maps was unexpected. Several studies have reported high levels of segregation distortion, greater than 15% of loci at P < 0.05, within hybrid maps across a variety of taxa, including interspecific crosses between Mimulus guttatus and M. nastutus, Lepomis cyanellus and L. megalotis, and Nasonia giraulti and N. vitripennis and between intraspecific crosses of Salvelinus alpinus, M. guttatus, Ceratodon purpureus, Coregonus clupeaformis, and Tigriopus californicus. Divergence time estimates between parental lineages used for several of these maps ranged from 0.1-0.2 MYA to 13–16 MYA[60–63]. We would expect comparable distortion levels to these studies following the estimated 3 million year divergence time between RBT and YCT. Our findings are probably not due to a lack of power for detecting distortion, because we mapped a similar number of markers per linkage group as the studies above, although the number of individuals per mapping panel was less than these studies, except Woram et al.. Nevertheless, it is intriguing that segregation distortion was limited and few consistent distortion patterns emerged between sexes and families. The low level of distortion suggests that few incompatibilities exist between RBT and YCT, and may partially explain why these species readily form hybrid swarms.
Although segregation distortion was limited, we found a few noteworthy cases. A variety of mechanisms may cause distortion[14, 64, 65]. Understanding the causes of distortion typically requires in-depth study, but we discuss two mechanisms. First, pseudolinkage likely caused allelic distortion within two homeologous linkage groups in Male 2 (RYHyb07/RYHyb18 and RYHyb06/RYHyb27). Pseudolinkage may be implicated when homeologous linkage groups both show allelic segregation distortion, and we observed this distortion in YCT allele frequencies. Second, meiotic drive could have caused distortion. Meiosis is asymmetric in females and results in one functional haploid gamete per germ cell, compared to males where symmetric meiosis results in four functional gametes per germ cell. Therefore, any process that results in non-random segregation of chromosomes during oogenesis may lead to distortion in females. Female meiotic drive is characterized by competition among centromeres for spindle fiber attachment during meiosis and oogenesis. Because the centromere on one chromosome may outcompete the centromere on the homologue for deposition into the oocyte, allele frequencies in female maps may be distorted at loci mapping near centromeres. Male meiotic drive is characterized by competition between alleles during sperm development and results in differential gamete success. Genomic divergence between species could escalate competition between meiotic drive elements, causing segregation distortion in their hybrid progeny. Consistent distortion patterns within RYHyb01 between female hybrid maps and within RYHyb09 between male hybrid maps suggest that meiotic drive could be acting within the hybrids.
Conservation of synteny with the RBT sex chromosome, Omy1, suggests that RYHyb28 is the sex-linkage group. Indeed, sex has been established as mapping to homologs in YCT and RBT. Conservation of sex-linkage groups is uncommon across several salmonid species[20, 69–71]. Two alternative mechanisms have been proposed to account for the lack of conservation among sex-linkage groups in salmonids: either the sex-determining gene is the same among species but has moved to different chromosomes in different lineages, or sex determination has evolved independently in different lineages. Regardless of the mechanism, the lack of homology among sex chromosomes could be an important factor for restricting inter-specific hybridization, and could explain why hybridization is not commonly observed between many sympatric salmonid species and, conversely, why two species pairs identified as having homologous sex-linkage regions, YCT and RBT and Arctic charr (S. alpinus) and brook charr (S. fontinalis), introgress in the wild[73, 74]. The relationship between conservation of sex chromosomes and introgression is confounded in brown trout (Salmo trutta) and Atlantic salmon (S. salar) because their sex chromosomes lack homology, yet the species hybridize in the wild. However, brown trout and Atlantic salmon hybrids suffer reduced viability and introgression is rare. Nevertheless, homology between sex-linkage groups could possibly facilitate introgression between RBT and YCT.
Our results are consistent with a growing number of studies demonstrating that chromosome rearrangements reduce gene flow by suppressing recombination[10, 11, 30, 43, 49, 50, 52]. Although much of the RBT and YCT genome appears porous to gene exchange, our study indicates that chromosome rearrangements between RBT and YCT act as genomic obstacles to introgression. As a consequence, chromosome arrangements could have a significant influence on the evolution of YCT-RBT hybrid genomes. For example, within admixed populations, rearrangements could protect particular YCT genomic regions from RBT introgression, enabling large segments of the YCT genome to remain intact. If fitness related genes are linked to these rearrangements, recombination suppression could preserve them from being disrupted. This could provide an adaptive advantage to hybrids that contain these fitness related genes and enable these genes to persist within admixed populations. In contrast, unobstructed gene flow between chromosomes with similar arrangement would disrupt linkage associations within each species and create new genetic variation for selection to act upon.
The genetic linkage map established herein for YCT-RBT F1 hybrids provides an initial framework for investigating hybridization and is likely relevant to other cutthroat trout subspecies that introgress with RBT, and, therefore, may serve as a general model of genomic introgression. In addition, our study defines a set of genome-wide species markers that can be applied to conservation and management of indigenous YCT.
Hybrid F1 YCT-RBT parents were generated by crossing female YCT collected from Henry’s Lake Fish Hatchery and Fish Management Station, Idaho Department of Fish and Game (IDFG), with male RBT (Kamloops stock) from Hayspur Hatchery (IDFG). Mature F1 hybrids were collected at Henry’s Lake in March of 2004 and used to generate two F2 hybrid full-sib crosses (Family 1, N = 54 mapping progeny; Family 2, N = 53 mapping progeny). Because a YCT genetic map does not exist, we also constructed a YCT cross (N = 48 mapping progeny) so that we could clarify linkage anomalies observed between the F1 hybrid and published RBT maps[21, 33]. Fin tissues were sampled from the parents and we confirmed the hybrid/species status of F1 hybrid and YCT parents by screening 12 species-specific markers that differentiate RBT and YCT (Additional file1, Worksheet 1). Crosses were reared 10 months post-fertilization at which point the fish were euthanized and fin tissues sampled. DNA was extracted using DNeasy kits (Qiagen Inc., Valencia, CA, USA).
Rainbow trout genetic linkage maps developed by Guyomard et al. and Rexroad et al. served as templates for F1 hybrid map construction. We applied 294 microsatellite primers (Additional file1, Worksheet 1), spanning a large portion of each RBT linkage group and ensuring coverage across the centromere for metacentric chromosomes. Microsatellite amplification and PCR product visualization followed the methods of McClelland and Naish.
We also applied 169 SNPs (Additional file1, Worksheet 1). SNPs were interrogated using TaqMan 5’ nuclease assays (Applied Biosystems Inc., Carlsbad, CA, USA) or SNPtype assays (Fluidigm Corporation, San Francisco, CA, USA). All genotyping was carried out in 96.96 Dynamic Genotyping Arrays on an EP1 Genotyping System (Fluidigm Corporation), with a pre-amplification step, following manufacturer’s protocols. Genotypes were determined using the Fluidigm SNP Genotyping Analysis software (v 3.0.2), with confidence threshold set to 80%.
In addition, 14 insertion/deletion and four RFLP species-diagnostic primers (Additional file1, Worksheet 1) were included in the map. Amplifications were performed in 20 μl reaction volumes consisting of 15 ng genomic DNA, 1X NH4 Reaction Buffer (Bioline, Taunton, MA, USA), 1.5-2.5 mM MgCl2, 200 μM each dNTP, 1.5 pmol of each primer, and 0.5 units Taq polymerase (Bioline, Taunton, MA, USA). PCR products were visualized on 2-4% agarose gels stained in ethidium bromide.
Linkage maps were established using LINKMFEX v2.3 software package with an LOD threshold at 3.0. Because male salmonids show less recombination across the genome than females, we initially constructed parent-specific linkage maps. Hereafter, parent-specific linkage maps are referred to as Female 1 (Family 1 female parent), Male 1 (Family 1 male parent), Female 2 (Family 2 female parent), and Male 2 (Family 2 male parent). Salmonids have high crossover interference and typically have one or no crossovers per chromosome arm; we therefore used the pairwise recombination fraction, theta (Θ), between adjacent markers to estimate map distances. Male and female maps were compared by generating sex-specific and sex-merged maps using LINKMFEX. The total number of linkage groups in the F1 hybrid map was determined from sex-merged maps. Linkage maps were graphically represented using the program MAPCHART. Markers that were heterozygous for the same alleles in both parents were excluded from the map because these parental genotypes reduce the number of informative progeny, generate missing data, and reduce mapping power for these markers. All species-diagnostic SNPs, indels, and RFLPs were heterozygous in both of the F1 hybrid parents, and thus these markers were not ordered within the map. However, we did assign each of these markers to a specific linkage group.
Recombination rate analyses
We estimated the average recombination ratio across the genome and across each linkage group between parents within each family and between parents of the same sex using LINKMFEX. Significant differences in genome-wide and linkage group-wide recombination rates were identified by summing G-test values and degrees of freedom across each comparison.
To determine if introgression suppressed recombination rates, we generated a consensus female RBT map from Guyomard et al. and Rexroad et al. using recombination distances between markers and compared recombination rates to the female-merged F1 map. The consensus female RBT map was generated using only markers in common with the female-merged F1 hybrid map (Additional file1, Worksheets 10). We ensured that maps had the same marker order among RBT and female-merged F1 hybrids by removing markers that differed in their rank in the order. Markers used to compare recombination rates generally covered a substantial proportion of each RBT linkage group and were not restricted to areas of low recombination (centromeric regions) or areas of high recombination (telomeric regions). Kosambi map distances from Rexroad et al. were converted to Θ map distances by applying the formula θ = 0.5(e4k − 1)/(e4k + 1), where k = Kosambi distance. Prior to generating the consensus female RBT map, we tested for significant differences in recombination distances between adjacent loci between the two female RBT maps for each linkage group. The two female RBT maps were merged into the consensus map using LINKMFEX. We then tested for significant differences in recombination rate between the female-merged F1 map and the consensus female RBT map across linkage groups against the null hypothesis of no difference in recombination rate. Significant differences in the recombination rate were identified as described above.
Segregation distortion analyses
We tested for allelic distortion using a sliding window analysis, because loci exhibiting segregation distortion often cluster together within the genome. We established a 25 centiMorgan (cM) interval for the sliding window, because linkage was not supported for loci greater than 25 cM in distance at the LOD = 3.0 threshold. Species-specific markers were used to specify the most likely F1 parent chromosome phases as either YCT or RBT using LINKMFEX. Locus-specific allele frequencies were tested for deviation from 1:1 Mendelian expectations by summing G-test values and degrees of freedom within each 25 cM interval.
For testing genotypic segregation distortion, we used the most likely allele phases obtained from LINKMFEX in the sliding window analysis to assign locus-specific genotypes in the F2 hybrid progeny as YCT homozygote (YCT/YCT), RBT homozygote (RBT/RBT), or heterozygote (YCT/RBT). Significant deviation from the expected 1:2:1 Mendelian genotypic proportions was determined for each locus by applying chi square tests followed by multiple comparison corrections using a false discovery rate (B-Y FDR) across all loci.
C. O. Ostberg: U.S. Geological Survey, Western Fisheries Research Center, 6505 NE 65th Street, Seattle, WA 98115 and School of Aquatic and Fishery Sciences, University of Washington, 1122 NE Boat Street, Box 355020, Seattle, WA 98105, USA, firstname.lastname@example.org L. Hauser: School of Aquatic and Fishery Sciences, University of Washington, 1122 NE Boat Street, Box 355020, Seattle, WA 98105, USA, email@example.com V. L. Pritchard: Department of Biological Sciences, University of Turku, 20014 Turku, Finland, firstname.lastname@example.org J. C. Garza: Southwest Fisheries Science Center, National Marine Fisheries Service and University of California, Santa Cruz, 110 Shaffer Road, Santa Cruz, CA 95060, USA, email@example.com K. A. Naish: School of Aquatic and Fishery Sciences, University of Washington, 1122 NE Boat Street, Box 355020, Seattle, WA 98105, USA, firstname.lastname@example.org.
The authors wish to acknowledge support from the staff at Henry’s Lake Fish Hatchery and Fish Management Station and Hayspur Hatchery, Idaho Department of Fish and Game. Jeff Duda assisted in fish care. Funding was provided by the U.S. Geological Survey and the National Marine Fisheries Service. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.
- Barton NH, Hewitt GM: Adaptation, speciation and hybrid zones. Nature. 1989, 341 (6242): 497-503. 10.1038/341497a0.View ArticlePubMedGoogle Scholar
- Burke JM, Arnold ML: Genetics and the fitness of hybrids. Annu Rev Genet. 2001, 35 (1): 31-52. 10.1146/annurev.genet.35.102401.085719.View ArticlePubMedGoogle Scholar
- Rieseberg LH, Baird SJE, Gardner KA: Hybridization, introgression, and linkage evolution. Plant Mol Biol. 2000, 42 (1): 205-224. 10.1023/A:1006340407546.View ArticlePubMedGoogle Scholar
- Dowling TE, DeMarais BD: Evolutionary significance of introgressive hybridization in cyprinid fishes. Nature. 1993, 362 (6419): 444-446. 10.1038/362444a0.View ArticleGoogle Scholar
- Rieseberg LH, Wendel JF: Introgression and its consequences in plants. Hybrid zones and the evolutionary process. Edited by: Harrison RG. 1993, New York: Oxford Univeristy Press, 70-109.Google Scholar
- Allendorf FW, Leary RF, Spruell P, Wenburg JK: The problems with hybrids: setting conservation guidelines. Trends Ecol Evol. 2001, 16 (11): 613-622. 10.1016/S0169-5347(01)02290-X.View ArticleGoogle Scholar
- Rhymer JM, Simberloff D: Extinction by hybridization and introgression. Annu Rev Ecol Evol Syst. 1996, 27: 83-109. 10.1146/annurev.ecolsys.27.1.83.View ArticleGoogle Scholar
- Fishman L, Kelly AJ, Morgan E, Willis JH: A genetic map in the Mimulus guttatus species complex reveals transmission ratio distortion due to heterospecific interactions. Genetics. 2001, 159 (4): 1701-1716.PubMed CentralPubMedGoogle Scholar
- Fitzpatrick BM, Johnson JR, Kump DK, Shaffer HB, Smith JJ, Voss SR: Rapid fixation of non-native alleles revealed by genome-wide SNP analysis of hybrid tiger salamanders. BMC Evol Biol. 2009, 9: 176-10.1186/1471-2148-9-176.PubMed CentralView ArticlePubMedGoogle Scholar
- Rieseberg LH, Linder CR: Hybrid classification: insights from genetic map-based studies of experimental hybrids. Ecology. 1999, 80 (2): 361-370. 10.1890/0012-9658(1999)080[0361:HCIFGM]2.0.CO;2.View ArticleGoogle Scholar
- Panithanarak T, Hauffe HC, Dallas JF, Glover A, Ward RG, Searle JB: Linkage-dependent gene flow in a house mouse chromosomal hybrid zone. Evolution. 2004, 58 (1): 184-192.View ArticlePubMedGoogle Scholar
- Rieseberg LH, Linder CR, Seiler GJ: Chromosomal and genic barriers to introgression in Helianthus. Genetics. 1995, 141 (3): 1163-1171.PubMed CentralPubMedGoogle Scholar
- Stevison LS, Hoehn KB, Noor MA: Effects of inversions on within- and between-species recombination and divergence. Genome Biol Evol. 2011, 3: 830-841. 10.1093/gbe/evr081.PubMed CentralView ArticlePubMedGoogle Scholar
- Danzmann RG, Gharbi K: Gene mapping in fishes: a means to an end. Genetica. 2001, 111 (1): 3-23.View ArticlePubMedGoogle Scholar
- Buerkle CA, Rieseberg LH: Low intraspecific variation for genomic isolation between hybridizing sunflower species. Evolution. 2001, 55 (4): 684-691. 10.1554/0014-3820(2001)055[0684:LIVFGI]2.0.CO;2.View ArticlePubMedGoogle Scholar
- Bradshaw HD, Wilbert SM, Otto KG, Schemske DW: Genetic mapping of floral traits associated with reproductive isolation in monkeyflowers (Mimulus). Nature. 1995, 376 (6543): 762-765. 10.1038/376762a0.View ArticleGoogle Scholar
- Rieseberg LH, Raymond O, Rosenthal DM, Lai Z, Livingstone K, Nakazato T, Durphy JL, Schwarzbach AE, Donovan LA, Lexer C: Major ecological transitions in wild sunflowers facilitated by hybridization. Science. 2003, 301 (5637): 1211-1216. 10.1126/science.1086949.View ArticlePubMedGoogle Scholar
- Danzmann RG, Cairney M, Davidson WS, Ferguson MM, Gharbi K, Guyomard R, Holm L-E, Leder E, Okamoto N, Ozaki A: A comparative analysis of the rainbow trout genome with 2 other species of fish (Arctic charr and Atlantic salmon) within the tetraploid derivative Salmonidae family (subfamily: Salmoninae). Genome. 2005, 48 (6): 1037-1051. 10.1139/g05-067.View ArticlePubMedGoogle Scholar
- Gharbi K, Gautier A, Danzmann RG, Gharbi S, Sakamoto T, Hoyheim B, Taggart JB, Cairney M, Powell R, Krieg F: A linkage map for brown trout (Salmo trutta): chromosome homeologies and comparative genome organization with other salmonid fish. Genetics. 2006, 172 (4): 2405-2419.PubMed CentralView ArticlePubMedGoogle Scholar
- McClelland EK, Naish KA: A genetic linkage map for coho salmon (Oncorhynchus kisutch). Anim Genet. 2008, 39 (2): 169-179. 10.1111/j.1365-2052.2008.01699.x.View ArticlePubMedGoogle Scholar
- Rexroad C, Palti Y, Gahr S, Vallejo R: A second generation genetic map for rainbow trout (Oncorhynchus mykiss). BMC Genet. 2008, 9 (1): 74-PubMed CentralView ArticlePubMedGoogle Scholar
- Allendorf FW, Thorgaard GH: Tetraploidy and the evoution of salmonid fishes. Evolutionary genetics of fishes. Edited by: Turner BJ. 1984, New York: Plenum Press, 1-46.View ArticleGoogle Scholar
- Wright JE, Johnson K, Hollister A, May B: Meiotic models to explain classical linkage, pseudolinkage, and chromosome pairing in tetraploid derivative salmonid genomes. Isozymes Curr Topics Biol Med Res. 1983, 10: 239-260.Google Scholar
- Sakamoto T, Danzmann R, Gharbi K, Howard P, Ozaki A, Khoo S, Woram R, Okamoto N, Ferguson M, Holm L-E: A microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) characterized by large sex-specific differences in recombination rates. Genetics. 2000, 155: 1331-1345.PubMed CentralPubMedGoogle Scholar
- Phillips R, Rab P: Chromosome evolution in the Salmonidae (Pisces): an update. Biol Rev. 2001, 76 (1): 1-25. 10.1017/S1464793100005613.View ArticlePubMedGoogle Scholar
- Wilson WD, Turner TF: Phylogenetic analysis of the Pacific cutthroat trout (Oncorhynchus clarki ssp.: Salmonidae) based on partial mtDNA ND4 sequences: a closer look at the highly fragmented inland species. Mol Phylogenet Evol. 2009, 52 (2): 406-415. 10.1016/j.ympev.2009.03.018.View ArticlePubMedGoogle Scholar
- Loudenslager EJ, Thorgaard GH: Karyotypic and evolutionary relationships of the Yellowstone (Salmo clarki bouvieri) and west-slope (S. c. lewisi) cutthroat trout. J Fisheries Res Board Canada. 1979, 36 (6): 630-635. 10.1139/f79-091.View ArticleGoogle Scholar
- Thorgaard GH: Chromosomal differences among rainbow trout populations. Copeia. 1983, 1983 (3): 650-662. 10.2307/1444329.View ArticleGoogle Scholar
- Allendorf FW, Leary RF: Conservation and distribution of genetic variation in a polytypic species, the cutthroat trout. Conserv Biol. 1988, 2 (2): 170-184. 10.1111/j.1523-1739.1988.tb00168.x.View ArticleGoogle Scholar
- Machado CA, Haselkorn TS, Noor MA: Evaluation of the genomic extent of effects of fixed inversion differences on intraspecific variation and interspecific gene flow in Drosophila pseudoobscura and D. persimilis. Genetics. 2007, 175 (3): 1289-1306.PubMed CentralView ArticlePubMedGoogle Scholar
- Feder JL, Roethele JB, Filchak K, Niedbalski J, Romero-Severson J: Evidence for inversion polymorphism related to sympatric host race formation in the apple maggot fly, Rhagoletis pomonella. Genetics. 2003, 163 (3): 939-953.PubMed CentralPubMedGoogle Scholar
- Noor MAF, Grams KL, Bertucci LA, Almendarez Y, Reiland J, Smith KR: The genetics of reproductive isolation and the potential for gene exchange between Drosophila pseudoobscura and D. persimilis via backcross hybrid males. Evolution. 2001, 55 (3): 512-521. 10.1554/0014-3820(2001)055[0512:TGORIA]2.0.CO;2.View ArticlePubMedGoogle Scholar
- Guyomard R, Mauger S, Tabet-Canale K, Martineau S, Genet C, Krieg F, Quillet E: A Type I and Type II microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) with presumptive coverage of all chromosome arms. BMC Genomics. 2006, 7 (1): 302-10.1186/1471-2164-7-302.PubMed CentralView ArticlePubMedGoogle Scholar
- Phillips RB, Nichols KM, DeKoning JJ, Morasch MR, Keatley KA, Rexroad C, Gahr SA, Danzmann RG, Drew RE, Thorgaard GH: Assignment of rainbow trout linkage groups to specific chromosomes. Genetics. 2006, 174 (3): 1661-1670. 10.1534/genetics.105.055269.PubMed CentralView ArticlePubMedGoogle Scholar
- Phillips RB, Morasch MR, Wheeler PA, Thorgaard GH, Quattro JM: Rainbow trout (Oncorhynchus mykiss) of Idaho and Alaskan origin (2n = 58) share a chromosome fusion relative to trout of California origin (2n = 60). Copeia. 2005, 2005 (3): 661-664. 10.1643/CG-04-252R1.View ArticleGoogle Scholar
- Smukowski CS, Noor MA: Recombination rate variation in closely related species. Heredity. 2011, 107 (6): 496-508. 10.1038/hdy.2011.44.PubMed CentralView ArticlePubMedGoogle Scholar
- Pardo-Manuel De Villena F, Sapienza C: Recombination is proportional to the number of chromosome arms in mammals. Mamm Genome. 2001, 12 (4): 318-322. 10.1007/s003350020005.View ArticlePubMedGoogle Scholar
- Griffiths AJF, Wessler SR, Lewontin RC, Gelbart WM, Suzuki DT, Miller JH: Introduction to genetic analysis. 2005, New York: W. H: Freeman and Company, 8thGoogle Scholar
- Borodin PM, Karamysheva TV, Belonogova NM, Torgasheva AA, Rubtsov NB, Searle JB: Recombination map of the common shrew, Sorex araneus (Eulipotyphla, Mammalia). Genetics. 2008, 178 (2): 621-632. 10.1534/genetics.107.079665.PubMed CentralView ArticlePubMedGoogle Scholar
- Phillips RB, Kapuscinski ARD: A Robertsonian polymorphism in pink salmon (Oncorhynchus gorbuscha) involving the nucleolar organizer region. Cytogenet Genome Res. 1987, 44 (2–3): 148-152.View ArticleGoogle Scholar
- Britton-Davidian J, Sonjaya H, Catalan J, Cattaneo-Berrebi G: Robertsonian heterozygosity in wild mice: fertility and transmission rates in Rb(16.17) translocation heterozygotes. Genetica. 1990, 80 (3): 171-174. 10.1007/BF00137322.View ArticlePubMedGoogle Scholar
- Banaszek A, Fedyk S, Fiedorczuk U, Szałaj KA, Chetnicki W: Meiotic studies of male common shrews (Sorex araneus L.) from a hybrid zone between chromosome races. Cytogenet Genome Res. 2002, 96 (1–4): 40-44.View ArticlePubMedGoogle Scholar
- McGaugh SE, Noor MA: Genomic impacts of chromosomal inversions in parapatric Drosophila species. Philos Trans Royal Soc B. 2012, 367 (1587): 422-429. 10.1098/rstb.2011.0250.View ArticleGoogle Scholar
- Phadnis N, Orr HA: A single gene causes both male sterility and segregation distortion in Drosophila hybrids. Science. 2009, 323 (5912): 376-379. 10.1126/science.1163934.PubMed CentralView ArticlePubMedGoogle Scholar
- Rieseberg LH: Chromosomal rearrangements and speciation. Trends Ecol Evol. 2001, 16 (7): 351-358. 10.1016/S0169-5347(01)02187-5.View ArticlePubMedGoogle Scholar
- Ting C-T, Tsaur S-C, Wu C-I: The phylogeny of closely related species as revealed by the genealogy of a speciation gene, Odysseus. Proc Natl Acad Sci U S A. 2000, 97 (10): 5313-5316. 10.1073/pnas.090541597.PubMed CentralView ArticlePubMedGoogle Scholar
- Bidau CJ, Gimenez MD, Palmer CL, Searle JB: The effects of Robertsonian fusions on chiasma frequency and distribution in the house mouse (Mus musculus domesticus) from a hybrid zone in northern Scotland. Heredity. 2001, 87 (3): 305-313. 10.1046/j.1365-2540.2001.00877.x.View ArticlePubMedGoogle Scholar
- Dumas D, Britton-Davidian J: Chromosomal rearrangements and evolution of recombination: comparison of chiasma distribution patterns in standard and robertsonian populations of the house mouse. Genetics. 2002, 162 (3): 1355-1366.PubMed CentralPubMedGoogle Scholar
- Basset P, Yannic G, Brünner H, Hausser J: Restricted gene flow at specific parts of the shrew genome in chromosomal hybrid zones. Evolution. 2006, 60 (8): 1718-1730.View ArticlePubMedGoogle Scholar
- Yannic G, Basset P, Hausser J: Chromosomal rearrangements and gene flow over time in an inter-specific hybrid zone of the Sorex araneus group. Heredity. 2009, 102 (6): 616-625. 10.1038/hdy.2009.19.View ArticlePubMedGoogle Scholar
- Noor MA, Grams KL, Bertucci LA, Reiland J: Chromosomal inversions and the reproductive isolation of species. Proc Natl Acad Sci U S A. 2001, 98 (21): 12084-12088. 10.1073/pnas.221274498.PubMed CentralView ArticlePubMedGoogle Scholar
- Franchini P, Colangelo P, Solano E, Capanna E, Verheyen E, Castiglia R: Reduced gene flow at pericentromeric loci in a hybrid zone involving chromosomal races of the house mouse mus musculus domesticus. Evolution. 2010, 64 (7): 2020-2032.PubMedGoogle Scholar
- López-Fernández H, Bolnick DI: What causes partial f1 hybrid viability? Incomplete penetrance versus genetic variation. PLoS ONE. 2007, 2 (12): e1294-10.1371/journal.pone.0001294.PubMed CentralView ArticlePubMedGoogle Scholar
- Gadau J, Page RE, Werren JH: Mapping of hybrid incompatibility loci in Nasonia. Genetics. 1999, 153 (4): 1731-1741.PubMed CentralPubMedGoogle Scholar
- Woram RA, McGowan C, Stout JA, Gharbi K, Ferguson MM, Hoyheim B, Davidson EA, Davidson WS, Rexroad C, Danzmann RG: A genetic linkage map for Arctic char (Salvelinus alpinus): evidence for higher recombination rates and segregation distortion in hybrid versus pure strain mapping parents. Genome. 2004, 47 (2): 304-315. 10.1139/g03-127.View ArticlePubMedGoogle Scholar
- Hall MC, Willis JH: Transmission ratio distortion in intraspecific hybrids of mimulus guttatus: implications for genomic divergence. Genetics. 2005, 170 (1): 375-386. 10.1534/genetics.104.038653.PubMed CentralView ArticlePubMedGoogle Scholar
- McDaniel SF, Willis JH, Shaw AJ: A linkage map reveals a complex basis for segregation distortion in an interpopulation cross in the moss Ceratodon purpureus. Genetics. 2007, 176 (4): 2489-2500. 10.1534/genetics.107.075424.PubMed CentralView ArticlePubMedGoogle Scholar
- Rogers SM, Isabel N, Bernatchez L: Linkage maps of the dwarf and normal lake whitefish (Coregonus clupeaformis) species complex and their hybrids reveal the genetic architecture of population divergence. Genetics. 2007, 175 (1): 375-398.PubMed CentralView ArticlePubMedGoogle Scholar
- Pritchard V, Dimond L, Harrison JS, Velazquez C, Zieba J, Burton R, Edmands S: Interpopulation hybridization results in widespread viability selection across the genome in Tigriopus californicus. BMC Genet. 2011, 12 (1): 54-PubMed CentralView ArticlePubMedGoogle Scholar
- Bernatchez L, Chouinard A, Lu G: Integrating molecular genetics and ecology in studies of adaptive radiation: whitefish, Coregonus sp., as a case study. Biol J Linn Soc. 1999, 68 (1–2): 173-194.View ArticleGoogle Scholar
- Brunner PC, Douglas MR, Osinov A, Wilson CC, Bernatchez L: Holarctic phylogeography of Arctic charr (Salvelinus alpinus L.) inferred from mitochondrial DNA sequences. Evolution. 2001, 55 (3): 573-586. 10.1554/0014-3820(2001)055[0573:HPOACS]2.0.CO;2.View ArticlePubMedGoogle Scholar
- Campbell BC, Steffen-Campbell JD, Werren JH: Phylogeny of the Nasonia species complex (Hymenoptera: Pteromalidae) inferred from an internal transcribed spacer (ITS2) and 28S rDNA sequences. Insect Mol Biol. 1994, 2 (4): 225-237. 10.1111/j.1365-2583.1994.tb00142.x.View ArticleGoogle Scholar
- Near TJ, Bolnick DI, Wainwright PC: Fossil calibrations and molecular divergence time estimates in centrarchid fishes (Teleostei: Centrarchidae). Evolution. 2005, 59 (8): 1768-1782.View ArticlePubMedGoogle Scholar
- Pardo-Manuel De Villena F, Sapienza C: Nonrandom segregation during meiosis: the unfairness of females. Mamm Genome. 2001, 12 (5): 331-339. 10.1007/s003350040003.View ArticlePubMedGoogle Scholar
- Taylor DR, Ingvarsson PK: Common features of segregation distortion in plants and animals. Genetica. 2003, 117 (1): 27-35. 10.1023/A:1022308414864.View ArticlePubMedGoogle Scholar
- Fishman L, Willis JH: A novel meiotic drive locus almost completely distorts segregation in Mimulus (monkeyflower) hybrids. Genetics. 2005, 169 (1): 347-353.PubMed CentralView ArticlePubMedGoogle Scholar
- Fishman L, Saunders A: Centromere-associated female meiotic drive entails male fitness costs in monkeyflowers. Science. 2008, 322 (5907): 1559-1562. 10.1126/science.1161406.View ArticlePubMedGoogle Scholar
- Alfaqih MA, Phillips RB, Wheeler PA, Thorgaard GH: The cutthroat trout Y chromosome is conserved with that of rainbow trout. Cytogenet Genome Res. 2008, 121 (3–4): 255-259.View ArticlePubMedGoogle Scholar
- Davidson WS, Huang TK, Fujiki K, von Schalburg KR, Koop BF: The sex determining loci and sex chromosomes in the family Salmonidae. Sex Dev. 2009, 3 (2–3): 78-87.View ArticlePubMedGoogle Scholar
- Li J, Phillips RB, Harwood AS, Koop BF, Davidson WS: Identification of the sex chromosomes of brown trout (Salmo trutta) and their comparison with the corresponding chromosomes in Atlantic salmon (Salmo salar)and rainbow trout(Oncorhynchus mykiss). Cytogenet Genome Res. 2011, 133 (1): 25-33. 10.1159/000323410.View ArticlePubMedGoogle Scholar
- Woram RA, Gharbi K, Sakamoto T, Hoyheim B, Holm L-E, Naish K, McGowan C, Ferguson MM, Phillips RB, Stein J: Comparative genome analysis of the primary sex-determining locus in salmonid fishes. Genome Res. 2003, 13 (2): 272-280. 10.1101/gr.578503.PubMed CentralView ArticlePubMedGoogle Scholar
- Timusk E, Ferguson M, Moghadam H, Norman J, Wilson C, Danzmann R: Genome evolution in the fish family Salmonidae: generation of a brook charr genetic map and comparisons among charrs (Arctic charr and brook charr) with rainbow trout. BMC Genet. 2011, 12 (1): 68-PubMed CentralView ArticlePubMedGoogle Scholar
- Gunnell K, Tada M, Hawthorne F, Keeley E, Ptacek M: Geographic patterns of introgressive hybridization between native Yellowstone cutthroat trout (Oncorhynchus clarkii bouvieri) and introduced rainbow trout (O. mykiss) in the South Fork of the Snake River watershed, Idaho. Conserv Genet. 2008, 9 (1): 49-64. 10.1007/s10592-007-9302-6.View ArticleGoogle Scholar
- Hammar J, Dempson JB, Verspoor E: Natural hybridization between Arctic char (Salvelinus alpinus) and brook trout (S. fontinalis): Evidence from Northern Labrador. Can J Fish Aquat Sci. 1991, 48 (8): 1437-1445. 10.1139/f91-171.View ArticleGoogle Scholar
- Verspoor E, Hammar J: Introgressive hybridization in fishes: the biochemical evidence. J Fish Biol. 1991, 39: 309-334.View ArticleGoogle Scholar
- McGowan C, Davidson WS: Artificial hybridization of Newfoundland brown trout and Atlantic salmon: hatchability, survival and growth to first feeding. Aquaculture. 1992, 106 (2): 117-125. 10.1016/0044-8486(92)90196-R.View ArticleGoogle Scholar
- Thorgaard GH, Allendorf FW, Knudsen KL: Gene-centromere mapping in rainbow trout: high interference over long map distances. Genetics. 1983, 103 (4): 771-783.PubMed CentralPubMedGoogle Scholar
- Voorrips RE: MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered. 2002, 93 (1): 77-78. 10.1093/jhered/93.1.77.View ArticlePubMedGoogle Scholar
- Ott J: Analysis of Human Genetic Linkage. 1991, Baltimore: The Johns Hopkins University PressGoogle Scholar
- Benjamini Y, Yekutieli D: The control of false discovery under dependency. Ann Stat. 2001, 20 (4): 1165-1188.Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.