Phylogenetic and transcriptional analysis of an expanded bZIP transcription factor family in Phytophthora sojae
© Ye et al.; licensee BioMed Central Ltd. 2013
Received: 5 March 2013
Accepted: 14 November 2013
Published: 28 November 2013
Basic leucine zipper (bZIP) transcription factors are present exclusively in eukaryotes and constitute one of the largest and most diverse transcription factor families. The proteins are responsible for central developmental and physiological processes in plants, animals, and fungi, including the pathogenicity of fungal plant pathogens. However, there is limited understanding of bZIPs in oomycetes, which are fungus-like organisms in the kingdom Stramenopila. Oomycetes include many destructive plant pathogens, including the well-studied species Phytophthora sojae, which causes soybean stem and root rot.
Candidate bZIPs encoded in the genomes of P. sojae and four other oomycetes, two diatoms, and two fungal species were predicted using bioinformatic methods. Comparative analysis revealed expanded numbers of bZIP candidates in oomycetes, especially the Phytophthora species, due to the expansion of several novel bZIP classes whose highly conserved asparagines in basic DNA-binding regions were substituted by other residues such as cysteine. The majority of these novel bZIP classes were mostly restricted to oomycetes. The large number of novel bZIPs appears to be the result of widespread gene duplications during oomycete evolution. The majority of P. sojae bZIP candidates, including both conventional and novel bZIP classes, were predicted to contain canonical protein secondary structures. Detection of gene transcripts using digital gene expression profiling and qRT-PCR suggested that most of the candidates were not pseudogenes. The major transcriptional shifts of bZIPs occurred during the zoosporangia/zoospore/cyst and host infection stages. Several infection-associated bZIP genes were identified that were positively regulated by H2O2 exposure.
The identification of large classes of bZIP proteins in oomycetes with novel bZIP motif variants, that are conserved and developmentally regulated and thus presumably functional, extends our knowledge of this important family of eukaryotic transcription factors. It also lays the foundation for detailed studies of the roles of these proteins in development and infection in P. sojae and other oomycetes.
Transcription factors (TFs) are key nodes of regulatory networks that bind to the promoter regions of target genes and regulate transcription. The basic leucine zipper (bZIP) family of TFs is one of the largest and most diverse TF families found exclusively in eukaryotes. bZIPs can be identified based on the bZIP domain, which is 60 to 80 amino acids in length and consists of a basic region and a leucine zipper . The basic region is highly conserved and consists of approximately 16 amino acid residues with an invariant N-×7-R/K domain for nuclear localization and DNA binding. The leucine zipper mediates homo- and/or heterodimerization of bZIP proteins and is less conserved, consisting of a heptad repeat of leucine or other bulky hydrophobic amino acids, which is positioned exactly nine amino acids toward the C-terminus . When bound to DNA, bZIP monomers are long α-helices; the N-terminal half of the basic region inserts into the major groove of double-stranded DNA where it binds to specific sequences, while the C-terminal half of the leucine zipper mediates dimerization to form a superimposed coiled-coil structure [2–4].
The bZIP family is multifunctional across species, and in fungal plant pathogens several genes play a role in regulating pathogenicity. For example, in Magnaporthe oryzae, Moatf1 is required for full virulence as it regulates the transcription of laccases and peroxidases to disrupt reactive oxygen species-mediated plant defense . MoAP1 is another bZIP that mediates the oxidative stress response and is important for growth, development, and pathogenicity . In the soilborne fungal pathogen Fusarium oxysporum, the bZIP HapX mediates iron homeostasis and is essential for rhizosphere competence and virulence .
Oomycetes, which include many plant pathogens, are fungus-like organisms that are actually evolutionarily related to brown algae and classified in the kingdom Stramenopila . Currently, little is known regarding the functions and regulation of the bZIP family in oomycetes. Only Pibzp1, a bZIP family protein in Phytophthora infestans, has been characterized; it is known to interact with a protein kinase and is required for zoospore motility and plant infection . Notably, within the DNA-binding region of Pibzp1, an asparagine residue that is conserved in other eukaryotes is substituted with cysteine, which suggests that some bZIPs in oomycetes may be functionally distinct . Although the genomes of several oomycete plant pathogens have been sequenced [10–13], systematic identification and analysis of the whole bZIP family in comparison with non-oomycete organisms is currently lacking.
Phytophthora sojae, one of the most well-studied oomycete plant pathogens, causes stem and root rot of soybean and is responsible for serious crop losses worldwide . The draft sequence of the P. sojae genome has greatly accelerated the study of Phytophthora-host interactions . In this study, we developed a bioinformatics pipeline and identified a comprehensive set of bZIP family members in P. sojae and another eight species of oomycetes, diatoms, and fungi. In addition to the conventional bZIPs, several novel classes of bZIPs were identified, and their expansion contributed to a larger bZIP family in oomycetes, especially Phytophthora. In addition, phylogenies, secondary protein structures, and gene expression patterns were analyzed and compared between conventional and novel classes of bZIP candidates. These results provide important data for further functional studies of bZIPs and other transcription factor families, and increase our understanding of potential transcriptional regulatory mechanisms in oomycetes.
Results and discussion
Large numbers of bZIP candidates in P. sojae
To identify bZIP proteins encoded in the P. sojae genome, hidden Markov models (HMMs) of the bZIP domain were downloaded from the Pfam database  and used to search the 19027 predicted P. sojae protein sequences  using an e-value cutoff of 1e-5. Seventy proteins displayed matches, considerably more than the number of proteins annotated as containing “bZIP” domains in the P. sojae genome database (JGI v1.1, 23 genes)  or in the Fungal Transcription Factor Database (FTFD v1.2, 17 genes) . To validate the bZIP candidates obtained using the Pfam HMMs, the 70 matching proteins were analyzed using two online tools, namely the Batch CD-search tool (CDD, in NCBI)  and the SMART database . Based on these results, 34 proteins were confirmed to be bZIP candidates by at least one of those two tools. To identify the full set of P. sojae bZIP family proteins, the sequences of the bZIP domains from the 34 confirmed candidates were aligned to build a novel HMM, which was used to re-search the P. sojae protein sequences for further candidates. The process of rebuilding the HMM, confirming candidates with CDD and SMART, and then building a new HMM was repeated until no new proteins were identified. By this process, 45 bZIP TF candidates were obtained. A further 62 proteins were identified from the HMM predictions that were not confirmed by CDD or SMART (Additional file 1: Table S1).
Among the 45 candidates, over 30% exhibited substitutions in the conserved asparagine residue within the basic region of the bZIP domain (Additional file 1: Table S1), indicating that the P. sojae genome encoded some non-conventional bZIP or bZIP-like class proteins. We hypothesized that some additional bZIP proteins remained that were not detected using the above method that was originally developed for identifying conventional bZIP domains. Therefore, to search for additional variant bZIPs, the 45 full-length protein sequences were used to individually query the P. sojae protein sequences using the blastp program with a strict e-value (1e-40) cutoff. This search produced 34 new protein matches, 23 of which (68%) were present among the 62 sequences detected by the HMM predictions but not confirmed by CDD or SMART analyses, plus another 11 that were completely novel. bZIP domains within these 34 proteins were non-conventional and thus could not be directly predicted using CD-search or SMART. However, in 26 of the 34 proteins, bZIP domain regions could be manually identified from the HMM or blast alignments based on comparisons with conventional bZIP domains. Thus, these 26 candidates were added to the previous set of 45 proteins to form a set of 71 high quality P. sojae bZIP candidates for the purposes of the next steps in the analysis. The pipeline and a summary of the prediction process are listed in Additional file 1: Table S1. The gene models for the 71 high quality candidates were manually checked and errors in the predicted gene coding regions were corrected by mapping to expressed sequence tags (ESTs)  or according to well-characterized features of P. sojae introns, e.g., most introns are between 60 and 90 bp, and begin with GT and end with AG . Based on the coordinates in genome scaffolds, the 71 genes were arranged and named PsBZPc01 to PsBZPc71 (since the transcription factor functions of the encoded proteins have not been experimentally confirmed, we termed them P. sojae bZIP candidates, PsBZPc). Details of these genes are provided in Additional file 1: Table S2. The lengths of encoded proteins ranged from 167 to 829 amino acids (aa), with an average of 382 aa. Introns were absent from 48 genes (68%), while 11, 7, and 5 genes had 1, 2, and >2 introns, respectively.
Due to the somewhat arbitrary nature of the cutoff used to select the 71 high quality candidates, it is possible that some false positives that lack TF function may be included among these candidates, and furthermore that some highly variant but functional bZIP TF members may have been erroneously excluded.
Many oomycete bZIP candidates contain novel DNA-binding domains
Considering the two most highly conserved sites in the basic region of the bZIP domain, the conventional pattern of invariant residues was N-×7-R/K (termed the “N-R/K” bZIP class in this study), with N-R being the most frequent (N13 and R21 in the Pfam model; Figure 1B). In P. sojae, the two major bZIP classes were N-R/K/Y containing 30 proteins (43%; 23 having N-R) and C-R containing 20 proteins (28%) (Figure 1A, Additional file 1: Table S2). The bZIP previously reported in P. infestans, Pibzp1, is an example of the C-R class. The protein sequence of Pibzp1 was used to search the P. sojae bZIPs using Blastp. Six matches were found at an e-value cutoff of 1e-20 (PsBZPc25 was the best hit, e-value of 3e-53), all of which belonged to the C-R class (Figure 1A, Additional file 1: Table S2).
bZIPs form 2:1 complexes with target DNA , whereby each bZIP monomer makes specific contacts with cognate DNA half-sites via their basic regions, which form continuous long α-helices with C-terminal leucine zipper regions that promote dimerization . We submitted the full-length protein sequences of the 71 P. sojae bZIPs to the NPS@  Web server to predict α-helices. Most (>60) P. sojae bZIP domains were predicted to form a long α-helix without any break through the core basic and leucine regions (Additional file 2: Figure S1). No significant difference was found between the N-R and non-N-R class bZIPs in the prediction results. The presence of the expected secondary structure predictions in both conventional and novel P. sojae bZIPs supports that these are fully functional proteins.
Previous studies have shown that residues in the bZIP basic region are associated with DNA-specific recognition during protein-nucleic acid interactions. For example in mouse, a dominant mutation within the DNA-binding domain of the bZIP TF Maf causes murine cataracts and results in selective alterations to DNA binding . Replacement of invariant bZIP residues within the basic region of the yeast transcriptional activator GCN4 can also affect DNA-binding specificity . Furthermore, an arginine to lysine substitution in the bZIP basic region of the opaque-2 transcription factor in maize abolished its specific DNA binding . Therefore, the novel basic region substitutions in the presumptive DNA-binding domains of the oomycetes bZIPs may indicate that they have novel DNA binding specificities or even may interact with different kinds of molecules.
Selective expansion of novel bZIP classes in oomycetes
To survey the distribution of these novel classes of bZIP TFs in more species, the bZIP prediction pipeline, including the building and updating of species-specific HMMs, was used to predict bZIPs in four other oomycetes (Phytophthora ramorum, 60 candidates; P. infestans, 47; Pythium ultimum, 37; and Hyaloperonospora arabidopsidis, 25), two diatoms (Thalassiosira pseudonana, 20; Phaeodactylum tricornutum, 20), and two fungi (Fusarium graminearum, 25; M. oryzae, 25). Both oomycetes and diatoms belong to the kingdom Stramenopila [14, 29], while fungi are evolutionally distant from this kingdom. Data from the prediction process are provided in Additional file 1: Table S1, and detailed sequence data and comments on all candidates can be found in Additional file 1: Table S3.
Novel classes of putative bZIP TFs are mostly restricted to oomycete species
The above predictions in nine species were based on a pipeline that began with an HMM for conventional bZIP domains. As outlined in the detailed prediction results (Additional file 1: Table S1), few additional bZIPs were identified among the non-Phytophthora species after the first round of HMM searches. Among oomycete species, many non-N-R class candidates were found in the first round of HMM searches, while few non-N-R class candidates were found in non-oomycete species. Thus the novel classes of putative bZIPs appeared to be mostly restricted to oomycete species.
To explore whether non-N-R classes such as C-R were more widely distributed than in the nine species examined using the pipeline, we used an alignment of C-R class bZIP domains collected from P. sojae, P. ramorum, and P. infestans to construct an HMM and used the HMM to query the UniProtKB protein sequence database (http://www.uniprot.org), using the “hmmsearch” tool at the HMMER Web server . UniProtKB includes complete reference proteome sets from many species, though proteins from non-Phytophthora oomycete species hadn’t been included in UniProtKB when the searches were performed. For comparison, an alignment of N-R class domains from P. sojae, P. ramorum, and P. infestans was also used to create an HMM for querying UniProtKB. Based on a relaxed threshold (E-value: 1.0), the N-R HMM found 5976 matches from 512 species. The top six species were Oryza sativa, Zea mays, Homo sapiens, Takifugu rubripes, Mus musculus, and Arabidopsis thaliana, and each species had more than 120 matches (Additional file 1: Table S4). In contrast, the Phytophthora C-R HMM found only 430 matches from 125 species. The top six species were P. sojae, P. ramorum, P. infestans, Botryotinia fuckeliana, Paracoccidioides brasiliensis, and Saccharomyces cerevisiae, with 71, 50, 38, 11, 9, and 8 matches, respectively (Additional file 1: Table S4). Thus, the Phytophthora species had significantly more matches than any other species. As shown in Figure 2B, nearly all matches from Phytophthora species fell into non-N-R classes, e.g., C-R (52%) and R-R (24%). In the non-Phytophthora species, N-R (58%) and V-R (15%) classes accounted for the majority of matches, while the remainder belonged to “other.” Only one C-R class bZIP candidate (Uniprot ID: G9MT11) was found in a non-Phytophthora species, namely the ascomycete fungus Trichoderma virens. This result confirmed that the majority of novel bZIP classes were restricted to oomycete species and that some novel classes contained multiple members.
Since the sequences of oomycete bZIP domains were unusually diverse, to ensure that seeds (alignments) from Phytophthora did not affect the sensitivity to sequences from distant species, we used the HMM of the canonical Pfam bZIP domain (ID: PF00170) to search the UniProtKB database. This HMM identified both conventional and novel classes of bZIP domains during the above predictions (Additional file 1: Table S1). Although more matches (8059) from more species (748) were obtained with the Pfam HMM than using the Phytophthora-N-R HMM, no obvious differences were observed between the top matched species and domain classes. Also, the novel bZIP classes were only found in Phytophthora species (Additional file 1: Table S4, Figure 2B). However, one more gene was found containing a C-R class bZIP domain (Uniprot ID: E0W336, from the arthropod Pediculus humanus corporis). In addition, we used our pipeline to catalog all bZIP members in the genomes of Tr. virens and Pe. humanus corporis. However, only the single C-R class bZIP candidate already identified was found in each case. A phylogenetic tree was constructed based on aligned bZIP domains from bZIP candidates of P. sojae, Py. ultimum, H. arabidopsidis, T. pseudonana, and M. oryzae, as well as the two novel C-R domains from non-Phytophthora species. As shown in Additional file 3: Figure S2, the two non-oomycete C-R class candidates fell within fungal clades that mostly contained N-R bZIPs. On the other hand, all or nearly all C-R, R-R, N-K, and V-R bZIPs fell into distinct sub-clades, respectively, rather than into the N-R class (Additional file 3: Figure S2), suggesting that genes for these novel bZIP classes may have emerged early in oomycete evolution.
The expanded bZIP family may result from widespread gene duplication
Based on all-to-all blastp searches of the 71 candidates using a 1e-50 e-value threshold, 45 (63%) of the P. sojae bZIPs fell into 11 groups likely resulting from recent duplications (Figure 3). After mapping bZIPs onto the genomic scaffolds, many tandem duplications and duplications within/between scaffold(s) were inferred (indicated with asterisks in Figure 3). For example, PsBZPc24 to PsBZPc28 appeared to be duplicated in tandem within scaffold_15, and additional duplicates appear to have been translocated to scaffold_20 (PsBZPc31) and scaffold_8 (PsBZPc10) (assuming the apparent translocations do not result from sequence assembly errors). Most of the genes in group 2 (37/43; 86%) are located within sub-clades that correspond primarily to genomic clusters, supporting the inference that most of the group 2 sequences result from recent gene duplications. Several of these clusters are specific to P. sojae (e.g., PsBZPc55-c59 and PsBZPc15-c19) (Additional file 5: Figure S4; Figure 3) indicating that the duplications occurred subsequent to the radiation of P. sojae. Others however include bZIPs from other Phytophthora species (Additional file 5: Figure S4) (e.g., PsBZPc64 and PsBZPc65; PsBZPc37 and PsBZPc38) indicating that the duplication may have occurred prior to the separation of Phytophthora species. In contrast to group 2, only 8 of the 28 group 1 members (29%) show evidence for gene duplications and even in those cases the presence of orthologs of those genes in other Phytophthora or oomycete species (Figure 3; Additional file 3: Figure S2 and Additional file 5: Figure S4) suggests that the duplications predated the radiation of those species. The majority of the group 1 members show distinctive ortholog relationships with bZIPs from other Phytophthora and oomycete species consistent with a more ancient origin. Since the novel bZIP classes were located in distinct phylogenetic trees and exhibited substantially more frequent recent putative gene duplications, it seems plausible that they may have been recruited for functions distinctive to oomycetes, such as infection or zoospore development.
Zoosporangia, zoospore and cyst stages, and host infection correspond to major transcriptional shifts in bZIPs
Varied stage-specific expression patterns were observed. Based on hierarchical clustering (HCL), 11 gene expression clusters were obtained. As shown in Figure 4, four clusters encompassing 53 genes (75%) represented the major expression patterns of P. sojae bZIP candidates. Cluster-F (13%) exhibited reduced transcript levels in zoospore and cyst stages. Cluster-G (18%) exhibited elevated transcript levels in zoosporangia stage and reduced transcript levels in zoospore and cyst stages. Cluster-H (18%) exhibited highly elevated transcript levels during zoospore and cyst stages. Cluster-J (25%) exhibited elevated transcript levels in germinating cysts that remained high during infection. In addition, Cluster-I with 4 members exhibited high transcript levels during cysts and germinating cysts stages. These clusters indicated that the major transcriptional shifts of bZIPs occurred during the zoosporangia/zoospore/cyst and host infection stages.
When comparing the proportions of genes in groups 1 (28 genes) and 2 (43 genes) belonging to each gene expression cluster (Figure 4), group 1 had higher proportions of genes in cluster-F (18% vs. 9%), cluster-H (21% vs. 16%), and cluster-J (36% vs. 19%); group 2 had a higher proportion in cluster-G (23% vs. 11%). However, none of these differences were statistically significant (chi-square p > 0.10). In P. infestans, Pibzp1 was highly expressed during the mycelia stage, but repressed during the zoospore and cyst stages . In P. sojae, the homologous gene PsBZPc25 had a similar expression pattern (Figure 4).
Several infection-associated bZIPs were also regulated following H2O2treatment
In this study, a comprehensive set of bZIP transcription factor candidates encoded in the P. sojae genome were compared to similarly predicted sets from four other oomycetes, two diatoms, and two fungal species. The analysis revealed an expanded number of bZIP candidates in oomycetes, especially the Phytophthora species, primarily due to the expansion of several novel classes of bZIP candidates. The majority of novel bZIP classes were restricted to the oomycete species. Phylogenetic and genome location analyses indicated that the increased number of genes encoding the novel bZIP classes might have resulted from widespread gene duplications during oomycete evolution.
The novel bZIP classes are characterized by substitutions in residues within the basic region that are typically conserved in other organisms, and may play an important role in DNA-binding specificity. Therefore, the novel basic region substitutions in the presumptive DNA-binding domains of the oomycetes bZIPs may indicate that they have novel DNA binding specificities or even may interact with different kinds of molecules. One novel bZIP TF from P. infestans that has been well characterized, Pibzp1, is a member of the novel bZIP class, C-R, and has been shown to regulate motility and infection .
We examined transcription of the 71 P. sojae candidates to explore their possible functions, including comparison between the conventional and novel bZIP classes. Transcripts were detected from the majority of candidates using DGE and qRT-PCR, suggesting that most genes, including those encoding the novel bZIPs, are not pseudogenes. Consistent with this observation, the predicted secondary structures of both conventional and novel P. sojae bZIPs exhibited the expected alpha helical structures spanning the basic and leucine zipper regions. Given the diverse and important roles that bZIP TFs play in eukaryotic organisms, and the conservation of the novel bZIPs among oomycete species, it would have been surprising if these genes had turned out to be pseudogenes.
The major transcriptional shifts of bZIPs occurred during the zoosporangia/zoospore/cyst and host infection stages. Interestingly, the overall gene expression level of conventional bZIP classes was higher than that of novel classes, but the significance of the differences in gene expression patterns is not clear. Several genes that were induced during pre-infection and/or early infection were also transcriptionally regulated by H2O2 treatment, suggesting that the genes regulated by these TFs might contribute to infection by protecting the pathogen from the host oxidative burst. The expression of some of the novel oomycete-specific bZIP genes in association with developmental structures specific to oomycetes (heterokont zoospores and zoosporangia ) suggests that these bZIPs may directly control the development of these tissues, as has been validated for Pibzp1 .
The P. sojae bZIPs presumably interact with promoter elements to exert regulatory effects on P. sojae physiology. Recently, several promoter elements have been identified from genome-wide analyses of genes relevant to the spore and infection stages of P. infestans[34, 35]. In addition, the absence of a TATA box in the oomycete core promoter and the presence of other core promoter motifs distinct from those of model organisms have been reported . Future studies will be required to determine if the novel motifs identified in oomycete promoters interact with any conventional or novel bZIPs identified in this study.
In conclusion, the identification of large classes of bZIP proteins in oomycetes with novel bZIP motif variants, that are conserved and developmentally regulated and thus presumably functional, extends our knowledge of this important family of eukaryotic transcription factors. It also lays the foundation for detailed studies of the role of these proteins in development and infection in P. sojae and other oomycetes.
Prediction of bZIP candidates
Conventional HMM models of bZIP domains were downloaded from the Pfam database at pfam.sanger.ac.uk/clan/bzip . Sequences of P. sojae (v1.1), P. ramorum (v1.1), T. pseudonana (v3.0), and P. tricornutum (v2.0) protein were obtained from the DOE Joint Genome Institute (JGI) database (genome.jgi.doe.gov), P. infestans (release of 6/15/2009), F. graminearum (FG3), and M. oryzae (MG8) from the Broad institute database (http://www.broadinstitute.org), Py. ultimum (DAOM BR144; pug1) from the Pythium Genome Database (pythium.plantbiology.msu.edu), and H. arabidopsidis (v8.3) from the VBI Microbial Database (eumicrobedb.org) .
The “hmmbuild,” “hmmscan,” and “hmmsearch” tools in the Hmmer v3.0 package and/or its online server  were used to format the HMM models and search the proteomes. We used 1e-5 as a cutoff e-value when searching entire genomes for bZIPs and a cutoff of 1.0 when searching UniProtKB for homologs of oomycete bZIPs. Blastp with an e-value cutoff of 1e-40 was used to search for new proteins that were identical to previous candidates following the HMM prediction. The Online Batch CD-search tool (http://www.ncbi.nlm.nih.gov/Structure/bwrpsb/bwrpsb.cgi)  and SMART database (smart.embl-heidelberg.de)  were used to validate the predicted bZIP domains; we required high quality candidates to be validated by at least one of these two tools.
Analysis of bZIP sequences
Sequence alignments were generated using MUSCLE (http://www.drive5.com/muscle) . The sequence logos of bZIP domains were created using the HMMER online tool (hmmer.janelia.org/search/hmmsearch) . Phylogenetic maximum-likelihood (ML) trees were constructed using PhyML implemented in SEAVIEW (pbil.univ-lyon1.fr/software/seaview.html)  and graphically viewed using MEGA 5 (http://www.megasoftware.net) . The full-length protein sequences of the 71 P. sojae bZIPs were submitted to NPS@ (using the tool “Secondary structure consensus prediction” at npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl?page=/NPSA/npsa_seccons.html)  to predict α-helices.
Analysis of gene expression patterns
P. sojae ESTs were collected from the Oomycete Transcriptomics Database (http://www.eumicrobedb.org/transcripts) , and digital gene expression profiling data were collected from the Phytophthora transcriptional database (PTD v1.1, phy.njau.edu.cn/ptd) and the published tag data (NCBI: GSE29651) . Gene expression pattern clustering (using hierarchical clustering methods) and drawing of the heat maps were performed using MeV software (http://www.tm4.org/mev.html) .
SYBR green real-time RT-PCR
Primers for qRT-PCR assay were designed by Primer3 (http://primer3.wi.mit.edu) and blastn was used to check the specificity of the sequence. The primers used in this study are listed in Additional file 1: Table S5. Total RNAs were extracted using a PureLink RNA Mini Kit (Invitrogen USA), then were transcribed to cDNA using a PrimeScript RT-PCR Kit (Takara). SYBR green real-time RT-PCR reactions used the SYBR Premix ExTaq Kit (TaKaRa Inc., Dalian, China) and the ABI PRISM 7500 fast real-time PCR system (Applied Biosystems, USA). The 7500 System Sequence Detection Software (Version 1.4) was used to analyze the relative expression levels of each sample. The P. sojae actA gene (GeneID: 108986) was used as a reference to standardize the expression levels. Student’s t test (two tails) was performed to test the significance of differences between controls and treatments.
Availability of supporting data
All the supporting data are included as additional files.
We thank Xinyu Yang for providing assistance in preparing RNA. This work was supported in part by grants to Yuanchao Wang from China National Funds for Distinguished Young Scientists (31225022) and Special Fund for Agro-scientific Research in the Public Interest (201303018) of China; and by grants to Brett M. Tyler from the Agriculture and Food Research Initiative of the USDA National Institute of Food and Agriculture, 2007-35600-18530 and 2011-68004-30104 and from the US National Science Foundation MCB-0731969.
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